Objective:To investigate the expression of nuclear factor-?B(NF-?B)subunits P50 and c-Rel protein in primary cortical neurons of Wistar rats at different time points of oxygen glucose deprivation/reoxygenation(OGD/R).Methods:The neurons dissociated from the cortex of the neonatal rats were primary cultured and were identified by immunocytochemistry.OGD/R model was established.The study was divided into 6 groups according to different processing methods,including normal group,OGD 4 h treated,OGD 4 h/R 2 h treated,OGD 4 h/R 6 h treated,OGD 4 h/R 12 h treated and OGD 4 h/R 24 h treated groups.The expression of NF-?B P50 and c-Rel protein in neurons was examined by immunocytochemistry method and Western blotting.Results:(1)Immunocytochemistry detection targeting neuron specific enolase(NSE)and beta-Ⅲ tubulin confirmed that the cultured cells were neurons.(2)The expression of NF-?B P50 protein was significantly higher in OGD 4 h group than in control group(P

Objective To study the inhibitory effects of circular dumbbell decoy oligodeoxynucleotides targeting NF-?B on TNF-? expression in cortical neurons under the oxygen glucose deprivation/reoxygenation(OGD/R).Methods The cultured neurons dissociated from the cortex of neonatal rats were observed on the 7th day after culture.To make the ischemia/reperfusion model in vitro,the neurons were exposed to OGD 4 h before cultured in normal culture media.The neurons were divided into 5 groups according to different processing methods 2 h before exposure to OGD,including normal group,OGD4 h/R6 h treated,NF-?B decoy ODNs treated,scrambled decoy ODNs treated and TransfastFastTM Transfection Reagent treated groups.The protein level of NF-?B P65 in primary neurons was detected by Western blotting.The protein and mRNA levels of TNF-? were detected by immunocytochemical method and RT-PCR,respectively.Results NF-?B decoy ODNs could effectively inhibit the expression levels of NF-?B P65 protein,TNF-? mRNA and protein in neurons(P

Objective To investigate the expression of high mobility group A1 (HMGA1) in patients with lung squamous cancer and its clinical significance.Methods The expression of HMGA1 in 76 patients with lung squamous cancer (lung squamous cancer group) and 30 cases of control lung tissue (control group) was detected by SP method of immunohistochemistry and its correlation with clinical-pathological parameter was analyzed statistically.Results The positive expression rate of HMGA1 was 68.4％ (52/76)and 16.7％ (5/30) in lung squamous cancer group and control group respectively,and the difference was significant(P＜ 0.01).HMGA1 expression showed significant correlation with lymph metastasis and degree of differentiation in patients with lung squamous cancer (P ＜ 0.05).Conclusions The progression of lung squamous cancer may correlate with the increase of HMGA1 expression.It's promising for HMGA1 to become a new marker for diagnosis of lung squamous cancer.Clinical detecting of HMGA1 could help with prognosis and treatment-guiding of lung squamous cancer.

BACKROUND: Because the cortical somatosensory evoked potential(CSEP) is easily affected by the anaesthesia drugs, so it needs to be combined with the subcortex somatosensory evoked potential (subcortical SEP)which is less affected by the junk to monitoring the spinal orthomorphia so as to increase the accuracy.OBJECTIVE: To monitor the onset of stretch spinal injury during rhachial orthomorphia with Combined application of subcortical SEP and CSEP.DESIGN: A comparative study of two kinds of evoked potential.SETTING: Department of Orthopaedics of the Second Affiliated Hospital of Sun Yat-sen University.PARTICIPANTS: Totally 104 patients with spinal orthomorphia ,including 40 males and 64 females, aged from 5 to 19 years, were selected from the Department of Orthopaedics of Sun Yi-xian Hospital of Sun Yat-sen University from July 1997 to December 2001.INTERVENTIONS: A Nicolet Viking Ⅳ (NICDLET Company, USA) with intraoperativemonitoring software was used to Record the wave of subcortical SEP and CSEP. The peak and after-following peak of the median N45and tibial P37 cortical responses were tagged manually for peak latency and peak-to-peak amplitude measurements. Reduction of more than 50% in SEP amplitude compared with the baselines measurement was defined as deterioration.MAIN OUTCOME MEASURES: Changes of CSEP and subcortical SEP during the surgery.RESULTS: Data of all 104 patients was entered the final analysis. Among 104 cases, 31 had positive cortex somatosensory evoked potential, 11 had positive infra-cortex somatosensory evoked potential, meanwhile, 3 cases changed into normal after relative treatment, which was related with operation. Accurate of cortex recorded point was 75%, under cortex 92.2%, and combination of cortex and infra-cortex 96.7%.CONCLUSION: The SEP is easily affected by the anaesthesia drugs, but it has good signals to record, however, the subcortical SEP is the contrary condition. The monitoring by combined application of CSEP and subcortical SEP during the spinal surgery can greatly increase the reliability of result.

Objective To investigate the myocardial protective effects of rosuvastatin in patients with unstable angina pectoris undergoing coronary stent implantation.Methods Patients (90 cases) with unstable angina pectoris undergoing coronary stent implantation in No.215 Hospital of Shanxi Nuclear Industry from January 2014 to May 2016 were divided into observation group and control group,45 cases in each group.Patients in the observation group were treated with rosuvastatin,and patients in the control group were treated with atorvastatin.The levels of CK-MB,cTnⅠ,h-FABP,hs-CRP,TNF-α,INF-γ,IL-6,IL-10,and IL-18 were compared between two groups.Adverse drug reaction and major adverse cardiac events of two groups were recorded.Results After 24 h operation,the levels of CK-MB,cTnⅠ,hs-CRP,TNF-α,INF-γ,IL-10,and IL-18 of two group were significantly higher than those before operation (P ＜ 0.05),and those in observation group were significantly lower than control group (P ＜ 0.05).There was no difference in h-FABP between two groups before and after operation (P ＜ 0.05).The level of IL-6 in the observation group after operation was higher than that before operation,but the difference was not significant (P ＜ 0.05);The level of IL-6 in the control group was significantly higher than that before operation and that of observation group (P ＜0.05);The rates of drug adverse reactions and major adverse cardiac events were similar in two groups.Conclusion Compared with same doseatorvastatin,the effects of rosuvastatin in muscle damage and inflammatory response are better,and do not increase adverse reactions.

Objective To investigate features and surgical management of anal fistula and perianal abscess in infants. Methods 41 pediatric patients (less than 2 years of age) were reviewed retrospectively.Results All patients were male with mean age of 9.5 months. 40 cases were previous anorectal abscesses. The all fistulas were single. Their origin in the crypts was clearly identified in 35 cases. When anoscopy was routinely performed, abnormal development of crypts was found in 19 cases(46 4%). All sick infants received surgical management. No recurrences were observed in the postoperation.Conclusion There are distinctly different between in infants and in adults for perianal abscess and /or fistula-in-ano. The eradication of the anal fistula and perianal infection is most important in reducing recurrence of postoperation.

OBJECTIVE:To establish a method for determination of the principle components in streptomycin sulfate in Streptomycin sulfate/cod liver oil nasal drop.METHODS:UV spectrophotometry was use to determine the absorption of streptomycin sulfate at detection wavelength of 521 nm.The content of streptomycin sulfate was also calculated.RESULTS:The detection concentration of streptomycin sulfate has favorable linear correlation within 250～700 ?g?mL-1 (r=0.999 8).The average recovery was 99.75% (RSD=0.98%).CONCLUSION:The method is convenient,accurate and suitable for quality control of the preparation.

Objective To investigate the effect of electrical stimulation to cerebellar fastigial nucleus on expression of nuclear factor-kappa B (NF-кB) P50, tumor necrosis factor-α (TNF-α) and Bcl-xL mRNA in rats brain after cerebral ischemia-reperfusion. Methods Sprague-Dawley rats were randomly divided into normal control group (NC group), cerebral ischemia-reperfusion group (I/R group), fastigial nucleus stimulation (FNS) group, and fastigial nucleus lesion (FNL) group. A focal cerebral ischemia-reperfusion model was established with middle cerebral artery occlusion (MCAO). 7 and 14 days after operation, the infarct volume was measured, and the protein of NF-кB P50 in rats brain was detected with Western blotting; the expression of TNF-α and Bcl-xL mRNA was detected with RT-PCR. Results Compared with I/R group, the expression of NF-кB P50 protein increased in FNS group (P<0.05), with the decrease of expression of TNF-α mRNA (P<0.01) and increase of Bcl-xL mRNA (P<0.05), while the infarct size decreased (P<0.01). There was no significant difference between FNL group and I/R group for all the measurements (P>0.05). Conclusion FNS could induce the expression of P50 protein and Bcl-xL mRNA, and inhibit the expression of TNF-α mRNA, and reduce infarct size, which may associated with the neuroprotection of central nervous system from injury.

Many studies have been conducted at home and abroad about carotid artery stenting to treat carotid artery stenosis. However, it is limited by the complications such as perioperative ischemia. Carotid artery stenting with cerebral protection devices can omit the procedure to block blood flow and can know the treatment condition through angiography. The results of comprehensive data show that carotid artery stenting is an efficient treatment technique, and can reduce the biocompatible responses of the host. In addition, carotid artery stenting with cerebral protection devices is a safe and effective therapy for high-riskcarotid artery atherosclerotic stenosis in the elderly.

OBJECTIVETo observe the expression of peripheral blood CD4+ T lymphocyte apoptosis gene in rheumatoid arthritis (RA) patients with cold dampness type (CDT), and to explore its correlation with clinical indicators of RA.

METHODSSixteen RA patients with CDT (as the RA group) and 16 healthy subjects (as the normal control group) were recruited. CD4 T cell apoptosis rate was detected in the RA group and the normal control group using FCM. mRNA expressions of fas, fasL, caspase-3, caspase-8, bcl-2, and bax were detected using RT-PCR. Correlations between the expression of apoptosis gene and clinical activity indicators of RA (ESR, CRP, RF, CCP, integrals for Chinese medial symptoms, morning stiffness time, joint tenderness number, joint swelling number, DAS28-3) were analyzed.

RESULTSThe apoptosis rate of CD4+ T was significantly lower in the RA group than in the control group [(2. 6 +/- 0.9) % vs. (7.7 +/- 1.3) %, P < 0.01]. mRNA expression levels of fas, fasL, caspase-8, caspase-3, and bax mRNA of CD4+ T significantly decreased, but bcl-2 mRNA expression increased in the RA group (P < 0.01). The apoptosis rate of CD4+ T was negatively correlated with ESR (P < 0.05). The mRNA expression of caspase-8 was negatively correlated with joint swelling number (P < 0.05). The mRNA expression of bcl-2 was negatively correlated with integrals for Chinese medial-symptoms and joint function classification (P < 0.01, P < 0.05).

CONCLUSIONApoptosis obstacle exists in peripheral blood CD4 +T lymphocyte of RA patients, and is closely related to disease activity.

Apoptosis ; Arthritis, Rheumatoid ; diagnosis ; metabolism ; pathology ; CD4-Positive T-Lymphocytes ; Caspase 3 ; metabolism ; Caspase 8 ; metabolism ; Fas Ligand Protein ; Humans ; RNA, Messenger