Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Female ; Humans ; Male ; Middle Aged ; Periarthritis ; therapy ; Shoulder Pain ; therapy

BACKGROUND: Exercise affects glucose transport 4(Glut4) protein levels in the skeletal muscle, but different intensions of exercise effects are unknown to people. OBJECTIVE: To explore effects of different intensity exercise on Glut4 protein expression in the skeletal muscle of rats. DESIGN, TIME AND SETTING: The randomized control animal experiment was performed at the Central Laboratory of Liaoning Normal University from September to October 2007. MATERIALS: A total of 60 healthy SD rats weighing (200?16) g of both genders were used in the following study. METHODS: Rat models of diabetes mellitus were created and divided into six groups: a normal control group, a diabetes mellitus control group and a diabetes mellitus group including 15 m/min group, 20 m/min group, 25 m/min group and 30 m/min group. Each group consisted of 10 rats. The exercise groups did the running exercise each day for 50 minutes, 6 days in a week, lasting for 6 weeks. MAIN OUTCOME MEASURES: Western bolt analysis was used to determine Glut4 protein on cell membrane of the skeletal muscle. Different intensions of exercise effects on body weight, blood glucose and insulin levels were observed in rats from each group. RESULTS: Compared with the diabetes mellitus control group, Glut4 protein contents enhanced up to 44.2% and 48% (P

Adult ; Ascorbic Acid ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Humans ; Infusions, Intravenous ; Male ; Myocarditis ; drug therapy ; virology ; Phytotherapy ; Virus Diseases ; drug therapy

Air Pollutants, Occupational ; analysis ; Cyanamide ; analysis ; Spectrophotometry ; Workplace

Brain ; Choristoma ; metabolism ; pathology ; surgery ; Female ; Glial Fibrillary Acidic Protein ; metabolism ; Humans ; Infant ; Neurofilament Proteins ; metabolism ; Orbit ; pathology ; surgery ; Orbital Diseases ; metabolism ; pathology ; surgery

Acupuncture Therapy ; Aged ; Angina Pectoris ; therapy ; Female ; Humans ; Male ; Middle Aged

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Adult ; Coronary Disease ; therapy ; Female ; Humans ; Male ; Middle Aged

Aim To study the expression of caspase-12 mRNA and protein following focal cerebral ischemia-reperfusion in rats,and explore the effect of endoplasmic reticulum pathway on neuronal apoptosis.Methods 60 male Wistar rats were randomly divided into sham-operated group and ischemic group.The middle cerebral artery occlusion(MCAO)models were established by using the intraluminal suture occlusion method,neuronal apoptosis was detected by TUNEL staining,the expression of caspase-12 protein was detected by immunohistochemical staining,the expression of caspase-12 mRNA was detected by RT-PCR method.Results In ischemic group,the number of apoptotic cells and the expression of caspase-12 mRNA and protein were gradually increased following prolonged cerebral reperfusion,reached the peak at 24 h.The number of apoptotic cells and the expression of caspase-12 mRNA and protein in ischemic group were significantly less than those of sham-operated group at all times(P

Guided by the antioxidant activity, the EtOAc-soluble and n-butanol-soluble fractions of the 50% methanol extract of Lonicera japonica leaves were isolated and purified by various chromatorgraphic methods, and the structures were identified by spectral analysis and comparison to the data reported in literature. As a result, nine compounds were obtained and identified as 5-O-caffeoylquinicacid (1), chlorogenicacid (2), 4-O-caffeoylquinicacid (3), luteolin-7-O-[α-L-arabinopyranosyl-(1 --> 6)] -β-D-glucopyranoside (4), luteoloside (5), 3,4-di-O-caffeoylquinic acid (6), 3,5-di-O-caffeoylquinic acid (7), 4, 5-di-O-caffeoylquinic acid (8) and luteolin (9). The antioxidant activity of the nine compounds were determined by using DPPH free radical scavenging method, and ascorbic acid was used as a positive control. Their antioxidant activities from high to low were 5 > 9 > 2 > 8 > 7 > 6 > 1 > 3 > 4. Among them, luteoloside (5) had the strongest antioxidant activity with an IC50 of 0.018 18 g x L(-1), and luteolin (IC50 0.023 6 g x L(-1)) and chlorogenicacid (IC50 0.035 17 g x L(-1)) ranks No. 2 and 3. Furthermore, the antioxidant activity of luteoloside and luteolin were stronger than that of ascorbic acid (IC50 0.027 54 g x L(-1)). These results gave a basis for the further study and utilization of L. japonica leaves.
Antioxidants ; chemistry ; isolation & purification ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Lonicera ; chemistry ; Mass Spectrometry ; Molecular Structure ; Plant Leaves ; chemistry

Bronchi ; pathology ; Bronchoscopy ; Female ; Foreign Bodies ; diagnosis ; Humans ; Middle Aged