BACKGROUND: Exercise affects glucose transport 4(Glut4) protein levels in the skeletal muscle, but different intensions of exercise effects are unknown to people. OBJECTIVE: To explore effects of different intensity exercise on Glut4 protein expression in the skeletal muscle of rats. DESIGN, TIME AND SETTING: The randomized control animal experiment was performed at the Central Laboratory of Liaoning Normal University from September to October 2007. MATERIALS: A total of 60 healthy SD rats weighing (200?16) g of both genders were used in the following study. METHODS: Rat models of diabetes mellitus were created and divided into six groups: a normal control group, a diabetes mellitus control group and a diabetes mellitus group including 15 m/min group, 20 m/min group, 25 m/min group and 30 m/min group. Each group consisted of 10 rats. The exercise groups did the running exercise each day for 50 minutes, 6 days in a week, lasting for 6 weeks. MAIN OUTCOME MEASURES: Western bolt analysis was used to determine Glut4 protein on cell membrane of the skeletal muscle. Different intensions of exercise effects on body weight, blood glucose and insulin levels were observed in rats from each group. RESULTS: Compared with the diabetes mellitus control group, Glut4 protein contents enhanced up to 44.2% and 48% (P

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Female ; Humans ; Male ; Middle Aged ; Periarthritis ; therapy ; Shoulder Pain ; therapy

Adult ; Ascorbic Acid ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Humans ; Infusions, Intravenous ; Male ; Myocarditis ; drug therapy ; virology ; Phytotherapy ; Virus Diseases ; drug therapy

Air Pollutants, Occupational ; analysis ; Cyanamide ; analysis ; Spectrophotometry ; Workplace

Brain ; Choristoma ; metabolism ; pathology ; surgery ; Female ; Glial Fibrillary Acidic Protein ; metabolism ; Humans ; Infant ; Neurofilament Proteins ; metabolism ; Orbit ; pathology ; surgery ; Orbital Diseases ; metabolism ; pathology ; surgery

Acupuncture Therapy ; Aged ; Angina Pectoris ; therapy ; Female ; Humans ; Male ; Middle Aged

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Adult ; Coronary Disease ; therapy ; Female ; Humans ; Male ; Middle Aged

Aim To study the expression of caspase-12 mRNA and protein following focal cerebral ischemia-reperfusion in rats,and explore the effect of endoplasmic reticulum pathway on neuronal apoptosis.Methods 60 male Wistar rats were randomly divided into sham-operated group and ischemic group.The middle cerebral artery occlusion(MCAO)models were established by using the intraluminal suture occlusion method,neuronal apoptosis was detected by TUNEL staining,the expression of caspase-12 protein was detected by immunohistochemical staining,the expression of caspase-12 mRNA was detected by RT-PCR method.Results In ischemic group,the number of apoptotic cells and the expression of caspase-12 mRNA and protein were gradually increased following prolonged cerebral reperfusion,reached the peak at 24 h.The number of apoptotic cells and the expression of caspase-12 mRNA and protein in ischemic group were significantly less than those of sham-operated group at all times(P

Objective To explore the clinical feature of acquired pure amegakdryocytic thrombocytopenic purpura(AATP).Methods 18 patients were analyzed retrospectively.And 112 patients with ITP in the same term were to be control group.Each group's megakaryocytic (MK),platelet (PLT) count,mean platelet volume (MPV) and platelet associated immunoglobulin G(PAIgG) were rested.Results The MK total number of AATP group was obvious decreased or even absent,while the ITP group was increased[(0.8?1.5) vs (195.0?47.3),P0.05).About the PAIgG posi- tive rate,AATP group was 55.6 % while ITP group was 83% (P

AIM: To assess the changes of macular thickness of acquire immunodeficiency syndrome ( AIDS) patients.
METHODS:The study based on the data analysis of 38 human immunodeficiency virus ( HIV ) positive patients ( 64 eyes ) . According to CD4 count and whether cytomegalovirus retinitis ( CMVR) happened, the patients were divided into 3 groups. Group A included 16 patients (32 eyes), in which CD4 count was < 50cells/μL and CMVR did not happen. Group B included 10 patients ( 20 eyes), in which CD4 count was>50cells/μL and CMVR did not happen. Group C included 12 patients (12 eyes), in which CD4 count was < 50cells/μL and CMVR just happened. Group D included 10 healthy people ( 20 eyes ) , as normal control group. By using optical coherence tomography ( OCT ) , thickness of retina along with a 1mm diameter circle centered on macular was evaluated.
RESULTS:The mean foveal thicknesses in groups A, B, C and D were 254. 03±15. 63μm, 263. 11±17. 12μm, 304. 50±50. 62μm and 257. 64±8. 54μm in order. Compared with foveal thickness in each group, there were significant differences in general (F=12. 933, P=0. 000). The mean foveal thickness in groups CMVR increased, which was of significant difference (P= 0. 000), compared with other groups.
CONCLUSION: CMVR can impair the structure and function of macular, which then seriously affects the visual function of patients. It's helpful to understand the progress and prognosis of CMVR disease by observing macular structure with OCT in early time.