Objective To investigate the relationship between the expression of Fas or FasL and the apoptosis of peripheral blood T and B lymphocytes in systemic lupus erythematosus (SLE) patients. Methods The expression rates of Fas and FasL and apoptosis rates of peripheral blood T and B lymphocytes were detected by flow cytometry (FCM) in 30 active SLE patients and 30 inactive SLE patients and 30 healthy controls. Results There were significant difference in the expression rates of Fas of T and B lymphocytes among three groups (P0.05). In addition, the activity of SLE had no correlation with the expression rates of Fas or FasL on T or B lymphocytes but has positive correlation with the apoptosis rates of T or B lymphocytes. And no correlation was found between the expression rate of Fas or FasL and the apoptosis rate of neither T nor B lymphocytes. Conclusion The apoptosis of peripheral blood lymphocytes is influenced by many factors in SLE patients, but is abnormally increased at last, especially in active SLE. It is inferred that the increase of apoptosis rate of peripheral blood lymphocytes plays an important role in the pathogenesis of SLE.

Objective To evaluate the effect of interactive case teaching(ICT) method in teaching hematology.Methods Sixty students were randomly selected from 2009 grade clinical medicine majors in the Third Military Medical University and were divided into experimental group and control group,each group with 30 students.Students in experimental group used ICT； while those in control group used traditional teaching method.Closed book exam was conducted for students in experimental group and control group at the end of the semester； question types of the exam included concept questions (nouns explain,choice,short answer questions) for 70 points,cases analysis question for 30 points,a total of 100 points.Data were expressed as mean ± standard deviation ((x) ±s) and analyzed with SPSS 20.0 independent sample t test.Questionnaire was designed and feedback from students was statistically analyzed.Results There was no statistical differences between experimental group and control group in concept questions (P =0.580),however,in terms of cases analysis question,scores in experimental group were significantly higher than those in control group (P =0.000) ； total scores of experimental group were also significantly higher than those in control group (P =0.016).Feedback showed that the students highly valued the form and effect of ICT.Conclusions Using ICT in teaching hematology can arouse students' enthusiasm for study,cultivate their independent thinking and ability of problem-solving.Meanwhile,ICT can combined theoretical teaching with clinical practice well and improve the quality of teaching.

Objective To investigate the effect of atorvastatin on the curative effect and cardiovascular events in patients with unstable angina pectoris.Methods128 patients with UAP were enrolled in Jinhua Central Hospital from August 2014 to August 2016, and 128 patients with UAP were randomly divided into observation group and control group.All the patients were given low salt and low fat diet.Two groups of patients were anti-platelet aggregation, blood pressure, lipid-lowering and other conventional treatment, observation group on the basis of simultaneous atorvastatin calcium 40mg/d, oral, once every month.The levels of TC, TG and LDL-C, HDL-C and the incidence of cardiovascular events were compared between the two groups before and after treatment.ResultsThere was no case of cardiac death in the observation group and the control group.The incidence of cardiovascular events in the observation group was 9.38%, which was significantly different from that in the control group (P<0.05).The levels of TC, TC and LDL-C in the observation group were significantly higher than those before treatment and control group, respectively.The clinical effect of the observation group was significantly better than that of the control group, the difference was significant (P<0.05).ConclusionAtorvastatin can significantly improve the efficacy of unstable angina treatment, and reduce blood lipid levels, reducing the incidence of cardiovascular events, improve the prognosis.

Objective To investigate the safety and superiority of fast track surgery (FTS)in elderly patients during the perioperative peri-od of laparoscopic cholecystectomy (LC).Methods The clinical data of 124 elderly patients who underwent LC at the Department of Gen-eral Surgery in our institution between January 2010 and March 2014 were assessed,with 62 cases assigned to FTS group and 62 cases to conventional method group.Anal exhaust time,feeding time,off-bed activity time,and length of hospital stay were compared between the two groups,and postoperative results and adverse reactions were recorded.Continuous data were analyzed using the independent-samples t test,and categorical data were compared using the chi-square test.Results Compared with the control method group,patients in the FTS group showed earlier postoperative anal exhaust,feeding,and off-bed activity,a shorter length of hospital stay,and a lower incidence of postoperative cardiovascular and cerebrovascular complications (P<0.05).Conclusion FTS is an effective approach to accelerate rehabil-itation in elderly patients after LC.

AIM: To compare the effects of histidine-tryptophan-ketoglurate(HTK)solution, University of Wisconsin(UW)solution and St.Thomas(ST) Cardioplegia solution Ⅱ on hypothermic preservation of isolated rat hearts. METHODS: Isolated hearts from male Wistar rats were mounted on a Longendorff apparatus to estimate left ventricular end-diastolic pressure(LVEDP),left ventricular developed pressure(LVDP), rate of change of left ventricular pressure(d p /d t max ,d p /d t min ) and coronary flow(CF).Twenty-four rats were randomly divided into three groups: HTK group, UW groups and ST group.They were arrest and stored at 4℃ in each preservation solution for 6 hours. Following storage, they were reperfused and postpreservative function was measured to assess cardiac functional recovery. Frozen samples were analyzed for concentration of adenosine triphosphate. RESULTS: The concentration of adenosine triphosphate in three groups was significantly different( P

Acupuncture Therapy ; Diarrhea ; therapy ; Female ; Humans ; Young Adult

Objective To compare the clinical features of space infections in the oral maxillofacial region between patients with diabetes mellitus and patients without diabetes.Methods A retrospective study was conducted on 61 patients diagnosed with space infections in the oral maxillofacial region and receiving treatment at the department of oral and The 4nd Affiliated Hospital of Kunming Medical University between 2013 and 2016.The patients were divided into 2 groups:diabetic group (n =28) and non-diabetic group (n =33).The study variables were demography,etiology,clinical and laboratory examinations (bacteriology,admission blood glucose level,admission white blood cell count and percent of neutrophil on admission).The outcome was compared.The length of hospital stay and complications were also compared.Statistical analyses of the results between groups were performed using the t test and chi square test.Results For oral and maxillofacial space infection,there was no statistically significant difference in gender between the diabetic group and (28 cases) and non diabetes group (33 cases) (x 2 test,P=0.5929);while there were statistically significant differences between two groups in age (t test,P＜0.001) and in the number of infection space (t test,P＜0.001);there was no significant difference between the two groups in the sources of infection (x 2,P＞0.05);The main source of infection was odontogenic,followed by adenogenous.There were statistically significant differences between two groups in blood glucose concentration at admission (t test,P＜0.001) and in the hospitalization time and complications (t test and x 2 test P＜0.05),while no statistically significant differences in the white blood cells count and neutrophil count (t test,P＞0.05).Compared with the non diabetic group,for patients in the diabetic group,the age of onset was older,the involvd gaps were more,the blood glucose concentration was higher on admission,the length of stay was longer,complications were more likely to occur,and the consequences were more serious.Conclusion Compared with non diabetes group,space infection in oral and maxillofacial region is more serious and the hospital stay is longer and has more complications in diabetic patients which should be paid higher attention to.

Objective To investigate the effect of Huoxuetongluo Decoction on expression of CD54 and polymorphonuclear adhesion during human umbilical vein endothelial cells(HUVEC) anoxia/reoxygenation injury,and explore its possible mechanisms.Methods Rabbits serum containing Huoxuetongluo Decoction was prepared.Resuscitate and culture HUVEC,then establish the anoxia/reoxygenation injury model of HUVEC.The model cells were divided into five groups:normal control,model group and group of high,medium,low dose of Huoxuetongluo Decoction.After dealt seperately,the morphologic change of cells were observed through the microscope,the expression of CD54 and the polymorphonuclear adhesion were determined.Results The group of low,medium and high dose of Huoxuetongluo Decoction inhibited the expression of CD54 and decreased the adhesion between polymorphonuclear and HUVEC,the effects were strengthened with increasing the dose of Huoxuetongluo Decoction.The difference between group of medium,high dose of Huoxuetongluo Decoction and model group had statistical significance(P

Objective To assess the feasibility of endoscopic endoluminal surgery. Methods Six pigs were used in the experiments. Percutaneous channels entering the gastric cavities were established and endo-gastric suturing was conducted under laparoscopy. Results All the six pigs had tolerated the surgery smoothly. Postoperative examinations by laparotomy found no leakage on suture or injuries of organs. The time for establishing endo-gastric channels, completing the suturing and total surgery were 60.5?12.2 min, 26.5?5.78 min and 92.1?15.0 min, respectively. Conclusions Endoscopic endoluminal surgery is technically feasible.

Objective To explore the feasibility of minimally invasive video-assisted thyroidectomy (M IVAT). Methods Clinical data of 8 consecutive patients with ben ign thyroid nodules treated by MIVAT in this hospital from September 2002 to Sep tember 2004 were retrospectively reviewed. Results All the pro cedures were successfully completed. The operative time was 90~150 min (mean, 12 0 min), and the hemorrhage volume, 10~30 ml (mean, 20 ml). No complications happ ened and no conversions to open surgery were needed. All the patients were compl etely recovered and discharged from hospital 3~4 days after the surgery. Follow- up checkups for 6~12 months (mean, 9 months) in the 8 patients found normally he aled wounds and no paraesthesia. Conclusions MIVAT is safe and effective, with excellent cosmetic outcomes.