BACKGROUND:MicroRNA has tissue and cel specificity, and high expression of endothelial cel-specific microRNA-126 (miR-126) plays an important role in angiogenesis.
OBJECTIVE:To explore the effect of miR-126 on transplanted free flap survival and histological activity as wel as its mechanism in angiogenesis.
METHODS:Transient transfection technology was used to enhance the expression of miR-126 in bone marrow mesenchymal stem cel s. Expression levels of macrophage inflammatory protein-1α, tumor necrosis factor-αand vascular cel adhesion molecule-1 mRNA were detected by real-time PCR, and expression levels of ERK1/2, AKT, pERK1/2, pAKT protein were measured by western blot assay. Forty-eight Sprague-Dawley rats were randomly divided into three groups (n=16 per group), fol owed by preparation of abdominal free flap models. Rats in the three groups were given injection of miR-126 mimics-transfected cel s, miR-126 mimics control-transfected cel s and PBS 1 cm and 3 cm distal to the free flap, respectively.
RESULTS AND CONCLUSION:Expression levels of macrophage inflammatory protein-1α, tumor necrosis factor-αand vascular cel adhesion molecule-1 mRNA in the cel s transfected with miR-126 mimics were decreased by 36, 3.5 and 14 times compared with those in the PBS group, respectively. Expressions levels of ERK1/2, AKT, pERK1/2, pAKT protein in the distal free flap increased significantly in the miR-126 mimics group than the other two groups, as did the ratios of pAKT/AKT and pERK1/2/ERK1/2. In addition, the expression levels of macrophage inflammatory protein-1α, tumor necrosis factor-αand vascular cel adhesion molecule-1 protein in the flap tissue fluid were significantly lower in the miR-126 mimics transfection group than the other two group. Al these findings suggest that miR-126 can promote free flap survival by creating favorable conditions for angiogenesis in the free flap tissue.

Asthma ; diagnosis ; Bronchitis ; diagnosis ; Child ; Diagnosis, Differential ; Humans

Emergencies ; Food Services ; organization & administration ; Foodborne Diseases ; prevention & control ; Humans ; Risk Assessment

A LMCWanimal model in cat was reproduced according to Carey′s method. Twelve cats were divided into two groups with 6 in each. In one group low velocity missile craniocerebral wound (LMCW) was produced (C group), and in another dextran 40 was given after the injury(Dex40 group). Pial microcirculation was observed in vivo under transluminal microscope, and pial microvessel caliber was also measured. Microregional cerebral blood flow (CBF) was measured with laser doppler flowmetry. Blood samples were collected at 5 time points for surveying and calculating blood rheological parameters. Brain tissues and brain microvascular corrosion casts were obtained for light and electronic microscope observation. There was vasoconstriction of the pial arterioles and venules immediately after the injury,lasting for about 30min. In C group, they dilated 1h later, and returned to normal at 2h,again vasoconstriction appeared in the arterioles from 5h to 8h, while there was vasodilatation of the venules. All blood rheological parameters rose significantly, and did not recover to normal after 8 hours. Petechial hemmorhage, swelling and damage of the nerve cells were found in the parietal lobe contralateral to the trauma. Microthrombosis and occlusion were observed in some microvessels. When the injured mice were treated with dextran 40 micro regional CBF and blood rheological changes were improved. We conclude that Dextran 40 ameliorates the cerebral microcirculation disturbance through improving the blood rheological changes following low velocity missile craniocerebral wounds, and Dextran 40 may be useful in clinical practice.

Humans ; Nanoparticles ; toxicity ; Skin ; drug effects ; Skin Absorption

Objective　To study the expression of p73 in human non-small cell lung cancer (NSCLC) and the relationship between p73 expression and clinico-pathological parameters. Methods　Expression of p7 3 gene was detected by RT-PCR in 32 human NSCLC tissues, tissues adjacent to ca ncer and non-cancer lung tissues. Results　p73 gene expression up-regulated substantially and detected in 87.5%(28/32) of human NSCLC tissues while expressed at low level in tissues adjacent to cancer and non-cancer lung tissues. Conclusion　Marked up-regulation of p73 gene expres sion is found in human NSCLC.

Biotrickling filter often offers a cost effective and environmentally friendly alternative to conventional air pollutant control technologies,but major problems with clogging of the filters due to a high biomass accumulation will provent it from the industried uses.In this paper,the effect of the high biomass accumulation in an air pollution treatment with a biotrickling filter is discussed.Two parameters with specific surface area with biofilm growth(?_ f ) and the bed porosity with biofilm(?_ f )are used to analyse its principle of accumulation.Finally,some control measures including chemical methods,physical machine-made methods and other control methods are overviewed.

OBJECTIVE To understand the status of the hospital infection and risk factors,as well as the existing problems of infection management,to improve the surveillance method of incidence rate of hospital infection and strengthen the infectious precaution and control measures.METHODS By efforts of the professional staff of hospital,with cooperateion of the clinic personnel infection management group,all the patients in hospital were investigated when sampled from zero hour of the day before investigation to the zero hour of the day of investigation,according to the requirement of nosocomial infection rate to design the questionnaire.The data were analyzed.RESULTS The patients investigated in hospital were 638,from them 38 cases with hospital infections were found,the incidence and case-time rates of hospital infection were 6.0% and 7.4%,respectively.Respiratory infection was the most common.CONCLUSIONS Compared with the investigation method used previously,the current approach is easy and standardized.It also provides the credible reference to improve infection surveillance and management.

Objective To compare the clinical outcomes of total laparoscopic pancreatoduodenectomy (TLPD) and open pancreaticoduodenectomy (OPD).Methods From August 2013 to December 2014,137 pancreaticoduodenectomies (PDs) were performed at the Department of General Surgery of our hospital,of which 19 TLPDs were performed at the Department of Minimally Invasive Surgery (the Experimental group).At the same time,another cohort of 19 OPDs were matched for age,gender,body mass index (BMI),ASA score and tumor site and they formed the control group.The clinical data in the perioperative period were evaluated,and the short-term clinical outcomes were compared.Results Operation time in the experimental group was significantly longer than the control group [(407.8 ± 146.5)min vs (263.3 ± 65.3) rmin,P ＜ 0.05].The mean intraoperative blood loss [(309.7 ± 151.2)ml vs (509.4 ± 309.9)ml],mean intensive care time after surgery [(47.5 ±16.8)h vs (68.1 ± 19.1)h],mean postoperative time to pass flatus [(3.5 ± 1.1) d vs (4.3 ± 1.1) d],mean postoperative hospitalization stay [(8.8 ± 2.1) d vs (10.8 ± 2.3)d] and mean incision length [(5.1 ± 0.9)cm vs (14.4 ± 1.3)cm] in the experimental group were significantly different from the control group (P ＜ 0.05).There were no significant differences on the intraoperative R0 resection rates,intraoperative pathology,tumor size [(20.6 ± 9.6) mm vs (25.9 ± 10.2)mm],number of lymph node dissected [(17.7 ± 6.5) vs (19.4 ± 5.6)],complication rates,recurrence rates and mortality between the two groups (P ＞ 0.05).Conclusions TLPD had comparable safety and therapeutic outcome when compared with OPD.Moreover,TLPD has the advantages of less bleeding,smaller wounds and faster postoperative recovery.TLPD requires specialized appliances and equipments,better surgeon experience and patient selection to achieve a high success rate.

Objective To study the relationship between vascular obstruction,inflammation and the pseudolobuli formation in liver tissues with chronic hepatitis B.Methods The liver biopsies from 1256 patients with chronic hepatitis B was observated by HE,histochemical,immunohistochemical and the electricity mirror techniqe.Results (1)The intrahepatic vascular inflammation and obstruction was a histopathological characteristics of liver cirrhosis of chronic hepatitis B;(2)The lesions of vascular inflammation caused the vascular obstruction and induced the proliferations of venous and fiber;(3)The blood vessel of hyperplasia revolved round the liver cell into the irregular cell circular(tiny node).The tiny node settled the composition base in the interest of pseudolobuli formation;(4)The blood vessel of hyperplasia once more inflammation,blocking,fiber deposit constituted the fiber brings,pseudolobuli formation.Conclusion The vascular obstruction,inflammation has a closely relationship with the pseudolobuli formation in liver tissues with chronic hepatitis.