Objective To explore clinical and neuroimagine features, as well as the management of rare ectopic pituitary adenoma in clivus. Methods We reported two cases of ectopic pituitary adenoma verified by histology in the clivus region, reviewed the relevant literature briefly, summarized the clinical and neuroimaging features, along with the management of ectopic pituitary adenoma in clivus. Results Ectopic pituitary adenoma in clivus caused symptoms of endocrinopathy and/or neurological dysfunction. Imaging studies showed an occupying lesion in the clivus. Pituitary adenoma was diagnosed in one patient preoperatively, the other was diagnosed as chordoma. The tumors were microsurgically removed through the trans-sphenoidal route. Tumor was radically removed in one patient, and during one-year follow-up, there was no recurrence. The other was partially resected, and suffered postoperative cerebrospinal fluid rhinorrhea and intracranial infection, follow-up of this patient lost finally. Conclusions The patients with ectopic pituitary adenoma in clivus have endocrinopathy or/and neurological dysfunction, and MRI plays a valuable role in the diagnosis. Although extremly rare, ectopic pituitary tumour needs to be considered in case of an occupying lesion in the clivus. Radical resection of tumor can cure the patient.

Objective To set up an antibody-capture ELISA method to detect the Japanese encephalitis virus(JEV)antibody.Methods ELISA plate was coated with the monoclonal antibody which was specific to the envelope protein epitope E39 of JEV,JEV SA14-14-2 strain as the source of antigen was used to absorb the monoclonal antibody,the absorbed virus used to capture the JEV'S antibody.The antibody that captured ELISA was established.The indirect ELISA method using the virus particles from cell culture was compared with coating ELISA plate,105 clinical serum were checked.Results The background in indirect ELISA assay could not be abscised,positive and negative serum diluted in a ratio of 1:10,1:100,1:1000,the relative value of A posative/A negative were 1.02,0.99,1.13,all<2.1.But the antibody-captured ELISA method when the serum dilution was 1:10,1:100,the A posative/A negative were 3.57,2.94,all>2.1;when the dilution was 1:1000,the A posative/A negative was 1.42,<2.1,it meant the method could distinguish the positive and negative serum efficiently when the dilution Was 1:100,the background problem in indirect ELISA assay could be solved.Antibody-capture method was used to check 105 serum samples,the A posative/A negative over a range of 0.257～0.321(0.262±0.050),all<2.1,no positive sample found.Conclusion The antibody-capture ELISA method has been preliminary set up with a high specificity,capable of quickly identifying JEV from other virus.

1 mm); A, the screw tip was adjacent to the aorta (≤1 mm); C, the screw tip was felt to be against the aorta and creating contour deformity. The angle composed of the central line of the screw and the central line of vertebral body on posterior X-ray film was also measured. Results The average correction of the main thoracic curve was 77.7% postoperatively. 155 screws were inserted, of which 134 screws(86.5%) had a bicortical purchase. 123 screws(79.4%) were distant from the aorta. Two screws encroached into the spinal canal. There were no significant difference between the proximal screws, the periapical screws and the distal screws. There were no vascular or neurologic complications or instrumentation failure during operation or follow-up. Conclusion Mini-open anterior correction for type Lenke 1 scoliosis enable a satisfy bicortical screw placement and curve correction. Sequential CT scan before surgery may improve the accuracy of screw placement, thus avoid the aortic or neurologic complication.

Objective To find out the state and characteristics of college students' mental health, and analyse the main factor affecting the mental health of college students. Methods This research investigated 1 350 college students sampled by random with the scale of Symptom Checklist 90(SCL-90), and compared the difference of mental health level between groups with the method of classifying comparison. Results The college students' total mean and factor score of SCL-90 were higher than those of youth norm in China, but 13.6% of college students had psychological problems worse than middle degree. After classifying comparison, the groups with higher score in SCL-90 total mean and factors were as follows: low grade, coming from single-parent family, having low degree of satisfaction with their specialty, feeling heavier stress in study, worse classmate relationship, not asking for help when having mental problem. Conclusion The factors which may affect the mental health level of college students are low grade, coming from single-parent family, having low degree of satisfaction with their specialty, feeling heavier stress in study, bad classmate relationship, and not asking for help when having mental problem.

RAPD analysis of eight soy sauce strains isolated from commercial soy sauce koji was done with random primers,using Aspergillus oryzae AS3.951 and Aspergillus sojae AS3.495 as controls.Six appropriate primers(Primer1,Primer5,Primer6,Primer7,Primer8,Primer9)for RAPD-PCR were screened from nine random primers,and repetitive experiments demonstrated that their RAPD-PCR polymorphic patterns were stable.There were usually 4～8 bands in their RADP-PCR patterns,where the number of the main bands was 1～4 and the secondary bands were abundant.The phylogenetic tree of these strains was reconstructed according to their RAPD-PCR patterns,and it basically corresponded to traditional morphological taxology,demonstrating that the application of RAPD molecular marker in the phylogenetic analysis of soy sauce strains was feasible.

Objective To summarize the experience on treatment of esophageal leiomyoma and mesenchymoma with video-assisted thoracoscope under the assistance of a fiber gastroscope.Methods The clinical data of 14 cases of esophageal leiomyoma and 2 cases of esophageal mesenchymoma treated with video-assisted thoracoscope under the assistance of fiber gastroscope from October 2004 to December 2009 was retrospectively analyzed.Selected different surgical path and operation hole position according to the lesion site.Fiber gastroscope was conductive to accurately find lesion positions,and detected esophageal mucosa was breaked or not.Extra-mucosal excision was conducted in 16 cases.Results Ihere was no death and severe complications during perioperative period.Patients were diagnosed by pathological examination.Thirteen cases were followed up for 6-48(18.5 ±9.5)months,all patients ate unobstructed,and without relapse.Conclusion Treatment of esophageal leiomyoma and mesenchymoma with video-assisted thoracoscope under the assistance of fiber gastroscope has the advantages of intraoperative accurate positioning,minimally invasive,thoroughly,fewer complications,and it is the first choice of esophageal benign tumor treatment.

[Objective]To evaluate diagnostic and the therapeutic options of osteoid osteoma of the spine associated with scoliosis. [Methods]Nine patients with osteoid osteoma of the spine were treated from 1997 to 2006.All patients presented with spine deformity as their main complaint at the first consultation.The mean age was 15.2 years (range 8-21 years).Two patients had their osteoid osteoma in the thoracic area,2 in the thorcolumbar area ,and 5 in the lumbar area.Three were well controlled after long term administration of nonsteroidal anti-inflammatory drugs,and the other 6 were treated with en bloc excision.[Results]All the 6 patients with en bloc excision of the tumor were pain-free post-operatively.The scoliotic curve disappeared or was less than 15 degrees in 5,but the structural curve persisted in 4.[Conclusion]The spinal osteoid osteoma should be suspected when back pain or painful scoliosis occurs in adolescents.Computed tomography is necessary to evaluate the location and features of the lesion.En bloc excision of the tumor can be suggested if the symptoms are not well relieved with nonsteroidal anti-inflammatory drugs.

0.05). Conclusion Thoracoscopic anterior spinal release have comparable clinical results as conventional thoracotomic release for AIS patients.

Objective To determine the effectiveness and clinical result of intraoperative monitoring of compound muscle action potential (CMAP) in avoiding nerve root impingement during lumbar spine intrapedical instrumentation. Methods Twenty-five cases were given intraoperative stimulus-evoked electromyographic monitoring. After anode of stimulating electrode was placed at the Kirschner wire inside the passage of pedicle screws, the integrity of the pedicle cortex was tested by stimulating each screw head and recording compound action potentials of relevant muscles. Results Stimulation at an intensity of 16.2 mA evoked a compound muscle action potential in one case and that at over 35 mA did in four cases. No neurologic deficits were found in any case intraoperatively or postoperatively. Conclusions Stimulating intensity below 10 mA needs rearranging screw site. A stimulation intensity of 10-20 mA indicates X-ray inspection and visual inspection to exclude any pedicle breaches. Stimulation thresholds above 20 mA can be regarded as safe threshold to monitor the nerve root impingement. Intraoperative electromyographic monitoring provides a real-time watch-dog for monitoring spinal nerve root injury during posterior lumbar instrumentation.

Objective To investigate the clinical outcome of intermittent distracting rod for the correction of juvenile scoliosis, and analyze its complications. Methods Between July 1997 through June 2002, 21 patients with scoliosis underwent posterior growing-rod instrumentation, with their age ranged from 6 to 11 years. Risser sign was 0 in all patients. Average curve magnitude was 78 degrees (62 to 94 degrees) before initial instrumentation. A pair of claw-type hooks were inserted at the upper and lower levels respectively, one rod was attached to the upper hooks and the other to the lower hooks. These two rods were allowed to overlap and were connected with a growth connector. Distraction was achieved by means of lengthening the two rods at the connector site. Repetition of lengthening was indicated by the curve deterioration larger than 15 degrees or the interval of 10 to 12 months after the previous operation. After each operation, a Milwaukee brace was worn for external support. Results The initial intraoperative correction averaged 40 degrees, but this decreased to 23 degrees of correction on average at every subsequent lengthening. The total distraction procedure of 4 times only achieved in 5 patients. The reasons for distraction ceasing were as follows: hook dislodgment in 3 patients, looseness of pedicle screw in 2, looseness of dominos connector in 2, wound infection in 1, spontaneous fusion in 2, and insufficient financial support in 6. Conclusion Curve response to correction tends to decline with consecutive distractions. A high rate of complications has been noted during the distraction procedure. The single distracting rod technique is not suitable for the correction of severe scoliosis in Chinese children.