2.Investigation of ethnic medicinal plants Orobanche, Cistanche and Boschniakia.
Zhen-Fang BAI ; Yong LIU ; Xiao-Qin WANG
China Journal of Chinese Materia Medica 2014;39(23):4548-4552
In this paper the species of ethnic medicinal plants Orobanche, Cistanche and Boschniakia, and their ethnopharmaceutical uses were comprehensively summarized by field investigation, systematical data analysis and comparison of relevant specimen and references. The results showed that six plants belonging to Orobanche were used as seven kinds of ethnic medicinal plants, two plants attributing Boschniakia were used as ten kinds of ethnic medicinal plants, two plants of Cistanche were used as three ethnic medicinal plants. The same plant was often used as different ethnic medicine in varied ethnic minorities. The effects of the ethnic medicines included yang-tonifying, hemostasis and analgesic activities. Hence, it is necessary to develop the rich plant resource of Orobanche for alleviation of Cistanche resources shortage.
Cistanche
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Orobanchaceae
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chemistry
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Orobanche
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chemistry
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Phytotherapy
4.A case with Coffin-Lowry syndrome.
Yong-ku LI ; Yu-jie LIU ; Zhen WANG
Chinese Journal of Pediatrics 2006;44(2):148-148
5.A convenient and stereoselective synthesis of (Z)-allyl selenides via Sm/TMSCl system-promoted coupling of Baylis-Hillman adducts with diselenides.
Yun-kui LIU ; Dan-qian XU ; Zhen-yuan XU ; Yong-min ZHANG
Journal of Zhejiang University. Science. B 2006;7(5):393-396
A simple and convenient procedure for stereoselective synthesis of (Z)-allyl selenides has been developed by a one-pot reaction of diselenides with Baylis-Hillman adducts in the presence of samarium metal-trimethylsilyl chloride under mild conditions. Presumably, the diselenides are cleaved by Sm/TMSCl system to form selenide anions, which then undergo S(N)2' substitution of Baylis-Hillman adducts to produce the (Z)-allyl selenides.
Magnetic Resonance Spectroscopy
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Molecular Structure
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Organoselenium Compounds
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chemistry
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Selenium Compounds
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chemical synthesis
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chemistry
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Stereoisomerism
6.Clinical characteristics and surgical treatment of ganglioneuroma in spine.
Yong HUANG ; Zhen-shan LYU ; Li-di LIU ; Di WU ; Li QIAO ; Shao-kun ZHANG
China Journal of Orthopaedics and Traumatology 2015;28(11):1013-1016
OBJECTIVETo summarize the clinical manifestation and diagnosis of ganglioneuroma in spine and investigate the clinical effect of surgical treatment.
METHODSThe clinical data of 6 patients underwent a surgery for ganglioneuroma in spine from January 2008 to January 2015 were retrospectively analyzed. There were 4 males and 2 females, aged from 2 to 63 years old with an average of 34.6 years. The courses of disease were from 3 days to 17 years. Five patients complicated with superficial hypesthesia in correlative level of tumor, and the muscle strength under tumor plane had decreased at different levels, with the strength of grade II-IV. Two cases complicated with hypermyotonia and positive bilateral Hoffmann's and Babinski sign. Five cases were sporadic lesion in correlative spinal canal and one case complicated with the giant occupying lesion in thoracic cavity.
RESULTSSix operations had been performed including 5 en bloc and 1 subtotal resection. Postoperative pathological results showed tumor cells scattered or fasciculated inserted into Schwann cells in the stroma. In 2 patients complicated with radiculalgia before operation, 1 case was relieved and 1 was invariant after operation. All 4 patients with preoperative dyscinesia in the limbs obtained improvement after operation. All the patients were followed up from 0.3 to 6.8 years with an average of 2.5 years. At the final follow-up, according to ASIA grade, 5 cases were good and 1 case was invariant. During the follow-up, only 1 patient experienced chemoradiation because of merging ganglioneuroblastoma and receiving subtotal resection. No recurrence in other 5 cases.
CONCLUSIONGanglioneuroma is a benign and rare tumors in spine. Clinically, radicular pain and sensory-motor disorders are the main manifestations. Its diagnosis depends on pathological examination. Prognosis of surgical treatment is good.
Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Ganglioneuroma ; diagnosis ; surgery ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Spinal Neoplasms ; diagnosis ; surgery
7.Studies on macroscopic and microscopic characteristics of Ophiocordyceps xuefengensis.
Hao LIU ; Lan-ping ZHEN ; Ru-cai ZHU ; Shui-han ZHANG ; Hui-yong HUANG
China Journal of Chinese Materia Medica 2015;40(14):2820-2824
The macroscopic characteristics, tissue, caterpillar body wall and powder of Ophiocordyceps xuefengensis in different batch numbers were observed and researched by the macroscopic and microscopic identification methods. The result shows that the morphology, size, abdominal annulations of caterpillar, etc. of 0. xuefengensis are the macroscopic identification characteristics, the caterpillar body surface mycelium, body wall sculpture and crochets on abdominal legs are the microscopic identification characteristics. These characters are stable and regular discriminant features, which are proved to be the identification basis of O. xuefengensis. In addition, The characters such as crochets on abdominal legs arrange in two parallel ellipse rings, the inner crochets are long strip, and the external toes are unciform, are specific.
Animals
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Hypocreales
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cytology
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Moths
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anatomy & histology
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cytology
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microbiology
8.Phenylethanoid glycosides distribution in medicinal plants of Gesneriaceae.
Zhen-Fang BAI ; Xiao-Qin WANG ; Pei-Gen XIAO ; Yong LIU
China Journal of Chinese Materia Medica 2013;38(24):4267-4270
To investigate the role of distribution and phylogeny of phenylethanoid glycoside in medicinal plants of Gesneriaceae, five phenylpropanoid glycosides, acteoside, paraboside B, isonuomioside A, paraboside II, and paraboside III were quantitatively determined in 12 species of Gesneriaceae by HPLC. The existence and content of these compounds were analyzed. The results showed that phenylethanoid glycosides were found in the most of those plants, but the kind of phenylethanoid glycosides varied in different species. Acteoside distribute in most of this plant group, paraboside B, isonuomioside A, paraboside II, and paraboside III were rare in those plants. The results of this study support morphological viewpoint that Trib. Trichosporeae is more developmental than Trib. Didymocarpeae.
Glucosides
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chemistry
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metabolism
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Magnoliopsida
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metabolism
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Phenylethyl Alcohol
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chemistry
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Plants, Medicinal
;
metabolism
9.Anticancer effect of 17-(6-cinnamamido-hexylamino-)-17-demethoxygeldanamycin: in vitro and in vivo.
Liang LI ; Hong LIU ; Sheng-Hua ZHANG ; Lei HU ; Yong-Su ZHEN
Acta Pharmaceutica Sinica 2013;48(12):1771-1777
In the present study, a new compound named 17-(6-cinnamamido-hexylamino-)-17-demethoxygeldanamycin (CDG) was obtained by introducing the cinnamic acid (CA) group into the 17-site of geldanamycin (GDM). The anti-cancer effects of CDG in vitro and in vivo were evaluated. MTT assay was used to examine the inhibitory effect of CDG on the proliferation of MCF-7, HepG2, H460 and SW1990 cells. Immunofluorescent staining flow cytometry combined with Annexin V-FITC/PI staining were used to detect apoptotic cells. Transwell assay was used to analyze the effect of CDG on cell invasion and migration ability. Western blotting was used to detect the expression levels of RAF-1, EGFR, AKT, CDK4 and HER-2 of MCF-7, HepG2 and H460 cells. The toxicities of CDG and GDM were evaluated in mice. Using the subcutaneously transplanted MCF-7 xenograft in nude mice, inhibitory effect was evaluated in vivo. The results showed that CDG inhibited the proliferation of cancer cells (IC50: 13.6-67.4 microg.mL-1). After exposure to CDG for 48 h, most cells presented typical morphologic changes of apoptosis such as chromatin condensation or shrunken nucleus. The rates of apoptosis of MCF-7, HepG2, H460 and SW1990 cells incubated with 10 microg.mL-1 CDG were 23.16%, 27.55%, 22.21%, 20.47%, respectively. A dose-dependent reduction of migration of four cell lines was found after exposure to CDG. The decreased levels of RAF-1, EGFR, AKT, CDK4 and HER-2 showed that CDG possessed HSP90 inhibitory effect. The result of animal toxicity test on the mice suggested that CDG had lower toxicity than GDM. Meanwhile, CDG inhibited the growth of MCF-7 xenografts of athymic mice.
Animals
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Antineoplastic Agents
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chemical synthesis
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chemistry
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pharmacology
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Apoptosis
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drug effects
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Benzoquinones
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chemical synthesis
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chemistry
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pharmacology
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Cell Line, Tumor
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Cell Movement
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drug effects
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Cell Proliferation
;
drug effects
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Cyclin-Dependent Kinase 4
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metabolism
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Female
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HSP90 Heat-Shock Proteins
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antagonists & inhibitors
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Humans
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Lactams, Macrocyclic
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chemical synthesis
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chemistry
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pharmacology
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Invasiveness
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Neoplasm Transplantation
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Proto-Oncogene Proteins A-raf
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metabolism
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Proto-Oncogene Proteins c-akt
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metabolism
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Random Allocation
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Receptor, Epidermal Growth Factor
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metabolism
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Receptor, ErbB-2
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metabolism
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Tumor Burden
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drug effects
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Xenograft Model Antitumor Assays
10.Lymphoepithelioma-like carcinoma of skin: report of a case.
Zhi-Yong WEI ; Song-Lin LIAO ; Xue-Mei ZHAN ; Feng-Zhen ZHANG ; Li-Li LIU
Chinese Journal of Pathology 2010;39(12):849-850