Objective To study the expressions of MCM5 and Ki67 in cervical intraepithelial neoplasia (CIN) and cervical cancer and their clinical significance. Methods Immunohistochemistry (S-P method) was used to detect the expressions of MCM5 and Ki67 in 50 CIN and 49 cervical cancer. Results The positive expression rate of MCM5 and Ki67 in normal cervical epithelium, CIN Ⅰ-Ⅱ, CIN Ⅲ and cervical cancer were 26.7%, 72.0%, 80.0%, 97.9% and 10.0%, 32.4%, 84.0%, 97.9% respectively. The positive expression rate of MCM5 was significantly different between CIN and cervical cancer (P

Objective:To observe the clinical treatment effects of Tangkang Xifang in the patients with type 0 diabetes foot ( DF) . Methods:Totally 73 patients with type 0 DF were randomly divided into the treatment group (n=37) and the control group (n=36). All the patients discontinued other medications for 7d before the treatment. Base on the blood glucose control with the traditional treat-ment by metformin hydrochloride (1. 5 g·d-1), the treatment group was additionally given warm water foot bath with Tangkang Xi-fang, while the control group was additionally given warm water foot bath, 30 min per time and twice a day for 60 days. The changes in the clinical symptoms, ankle brachial index ( ABI) , motor nerve conduction velocity ( MCV) and some other indices were measured before and after the treatment. Results:In comparison with the control group (58. 3%), the treatment group had higher total effective rate (86. 5%), and the usage of Tangkang Xifang also significantly improved the physiopathologic syndromes of peripheral artery dis-ease and neuropathy (P<0. 01). The ABI and MCV in the treatment group were significantly better than those in the control group (P<0. 05 or P<0. 01). Conclusion:Tangkang Xifang can effectively improve the clinical symptoms of type 0 DF, which is suitable for the clinical application.

Objective To investigate the imaging characteristics and to explore the clinical management of pancreatic duct stones. Methods The imaging characteristics and outcomes of different clinical management of 43 cases of pancreatic duct stones were retrospectively analyzed. Results In this group of patients with pancreatic duct stones, 42 cases were diagnosed by imaging examination, and 1 case was diagnosed during surgery. Among the 42 cases diagnosed by imaging, 20 cases were identified by B mode ultrasonography (BUS), 10 cases by CT, and 10 cases by ERCP. The pancreatic duct stones were located at the head of pancreas in 10 cases, at the body and tail of pancreas in 29 cases, and from the head to the tail of pancreas in 4 cases. Twenty patients with single pancreatic duct stones received conservative medical therapy, 6 patients with pancreatic duct stones located at the head of pancreas received endoscopic intervention therapy and 17 patients with multi pancreatic duct stones were successfully treated by surgery. Conclusions BUS, CT and ERCP are efficient means for diagnosing pancreatic duct stones. Choosing an appropriate management strategy based on the number, location and size of the stones is important for the successful treatment of pancreatic duct stones.

Objective To observe the expressions of apoptosis-related proteins Bcl-2 and Bax in cerebral ischemic preconditioning-perfusion models of rats. Methods Ischemic preconditioning was induced by 20 minutes of monolateral middle cerebral artery occlusion using an intraluminal filament method in rats. The animals were perfused 6 h, 12 h, 1d, 3 d, 5 d and 7 d respectively before they were sacrificed. The expressions of Bcl-2 and Bax were analyzed by immunohistochemistry staining. Results Bcl-2 positive cells increased significantly at 1 d after perfusion, reached their peak at 3 d, and maintained high level until 7 d. They were all higher than that of control group (all P 0.05). Conclusion Upregulation of Bcl-2 but not Bax may play a role in ischemic preconditioning protection .

Objective To investigate the role of apoptosis and apoptotic proteins p53,p21 and Bax in mechanisms of brain ischemic tolerance in rats.Methods In our study the models of focal-focal type brain ischemic preconditioning were maded by occlusion middle cerebral artery using an intraluminal filament method. Infarct sizes were measured by image analysis system.Neuronal apoptosis was identified by TUNEL staining and expressions of p53, p21 and Bax were analyzed by immunohistochemistry.Results Compared with the lethal ischemic group, the volume of infarct was greatly reduced when MCAO 20 minutes was performed as ischemic preconditioning( P

Objective:Understanding arthritis condition of rural residents of five countries in Liaoning province,analyzing their epidemiological factors,to further improve the prevention and treatment of arthritis and provide the basis for the promotion of Chinese medicine appropriate technologies.Methods:The cluster sampling method was used,at each of five counties in Liaoning we had surveyed 40 villages,and each village,we had spot-checked 50 families,the total amount was 2000.Each family member was a survey subject.Results:Among the five counties of Liaoning province,the incidence of arthritis in rural population was that women was higher than men,and 50-59 year-old age group had a higher proportion in arthritis,followed by 40-49 age group. Conclusion:Age and sex were also important factors for arthritic incidence of the five counties in Liaoning province.

Objective To study multi-slice spiral CT(MSCT) features of cardiogenic hepatohemia.Methods MSCT features of cardiogenic hepatohemia were retrospectively analysed.Results All cases were showed having reflux of contrast into the inferior vena cava(IVC)during arterial phase,of them,10 cases were accompanied with abnormal hepatic perfusion and 7 cases with intrahepatic lymphostasis sign.Conclusion The features including the reflux of IVC and abnormal hepatic perfusion during arterial phase in contrast-enhanced CT scan may suggest hepatohemia caused by higher pressure level of right heart from the cardiovascular diseases.

Objective To investigate the changes of T cells and disease development in the mice with Smad3 deficiency in the bone marrow cells.Methods Bone marrow cells obtained from Smad3 null(Smad3~(-/-))mice and wild type(Smad3~(+/+))mice were injected to Co~(60)-irradiated GFP mice,respectively.The general states of the bone marrow recipients were observed.The mice were sacrificed at the sixth week and the histopathological changes in the intestines were examined.The changes of T cells from lymph nodes were detected by flow cytometry.Results Smad3~(-/-) bone marrow recipient mice appeared a wasting syndrome and intestinal inflammation.The amount of CD4~+ CD62L~(lo) T cells in lymph nodes was significantly increased.Conclusion These results indicate that the mice with Smad3 deficiency in the bone marrow cells present an inflammatory disorder and their T cells are activated.

With the expanding scale of animal cell culture and increasing demand of biopharmaceutical,the development of serum-free medium based on cell lines and products has become a major task in the field of cell engineer.The statistical methods and tools are popular in the processes of design medium and can be used to evaluate the multiple factors and their interaction scientifically and effectively.The novel microarray and proteomic analysis can improve the performance of identifying the role of medium.The aim is to provide some idea for serum-free medium study through systematically summarizing the newly and commonly used serum-free medium study and design approaches and their characteristics.

Parkinson's disease(PD) is one of the most common neurological degenerative diseases with unknown causes and pathogenesis,and may be associated with genetic and environmental factors.Recent studies showed that PD had genetic basis.Genetics research of PD has been a hot topic nowadays,which provides hope for revealing the pathogenesis of PD.