Benzoates ; pharmacology ; Cells, Cultured ; Cellular Senescence ; drug effects ; radiation effects ; Gene Expression Regulation, Enzymologic ; drug effects ; radiation effects ; Humans ; Infant, Newborn ; Male ; Matrix Metalloproteinase 1 ; genetics ; Matrix Metalloproteinase 3 ; genetics ; Methoxsalen ; pharmacology ; RNA, Messenger ; genetics ; metabolism ; Retinoids ; pharmacology ; Skin ; cytology ; metabolism ; Time Factors ; Tissue Inhibitor of Metalloproteinases ; metabolism ; Ultraviolet Rays

OBJECTIVETo investigate effects of the variation of immune function in high humidity environment in different time, and lay a foundation for further study of the related mechanism.

METHODThirty SD rats were divided into 3 groups (n = 10): 20 day group, 40 day group in 90% relative humidity chamber and control group in normal relative humidity. Peripheral blood and spleens were collected to detect the levels of T lymphocyte subsets by Flow Cytometery.

RESULTSIn peripheral blood of the 20 day group rats, the CD3+ %, CD4+ %, CD8+ % and CD4+/CD8+ were 52.91 +/- 6.27, 37.80 +/- 4.11, 14.85 +/- 3.73 and 2.72 +/- 0.82 separately. Expect CD3+ %, they all had significant differences (P < 0.05). In addition, the data of the 40 day group rats showed no diversity in statistics. In spleen, CD8+ % of the 20 day group rats was 6.23 +/- 2.87 with significant differences (P < 0.05) and IgG, IgA and IgM did not change a lot in blood serum of the high humidity groups except C3 of the 20 days group (P < 0.05).

CONCLUSIONIn high humidity environment, the immune function of the rats increased in the initial stage. As time went on, the immune function gradually went to normal level through the self adjustment.

Acclimatization ; Animals ; Humidity ; Rats ; Rats, Sprague-Dawley ; Spleen ; immunology ; T-Lymphocyte Subsets ; immunology

Cholesterol-degrading strains was isolated from traditional koumiss. The effects of Lb. casei Zhang on the total serum cholesterol (TC) , triglycerid (TG), high density liporotein-cholesterol (HDL-C) low density liporotein-cholesterol (LDL-C) were investigated in artificially-induced hyperlipemial rats. The results showed that only heat-killed cells of Lb. casei Zhang significantly reduced serum TC (P

Objective To explore the influence of inhaled glucocorticosteroid on ?-glutamylcysteine synthetase(?-GCS) in inflammatory cell of sputum in children with asthma.Methods Twenty-two asthmatic children were divided into 2 groups according to treatment.The children who were treated by inhaled budesonide combined with salbutamol were due to group A and the others inhaling salbutamol only were due to group B,the healthy children were acted as healthy control group(group C).The glutation(GSH),total GSH and the activity of ?-GCS in sputum were measured respectively;Expression of ?-GCS in inflammatory cell of sputum were detected by immunohistochemistry;the expression of ?-GCS heavy chain(?-GCS-h) mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results 1.The total GSH[(1.08?0.14) ?mol/L] and oxidized glutathione(GSSG)[(0.37?0.09) ?mol/L] were decreased in sputum of group A of post-treatment compared with pre-treatment(Pa

This paper aims to investigate the regulatory mechanism of blood-activating and stasis-dissipating drugs on fecal metabolic characteristics of rhubarb-peach kernel in mice with adenomyosis (AM) using fecal metabolome method. Adenomyosis was modeled by pituitary transplantation, and after the end of modeling administration, fecal samples were collected from mice. Non-targeted metabolomics studies were performed using liquid chromatography-mass spectrometry (LC-MS) to compare the metabolic characteristics of the feces of mice in each group and to find intestinal differential metabolites and potential differential metabolic pathways. The results showed that compared with the normal group, 5-hydroxy-L-tryptophan, histidine, L-acetylcarnitine, 16-hydroxy hexadecanoic acid, thromboxane B₂, etc. were significantly up-regulated, L-urobilin and prostaglandin D₃ were down-regulated in the feces of the model group, and were reversed after treatment with the rhubarb-peach kernel. The results of metabolic pathway enrichment analysis showed that tryptophan metabolism and histidine metabolism were the main intervention pathways of the rhubarb-peach kernel on AM intestinal metabolism. This study found that the underlying mechanism of the rhubarb-peach kernel in the treatment of AM is related to the intervention of intestinal metabolism of tryptophan, histidine, bile acid, choline and arachidonic acid, and the regulation of pro-inflammatory microenvironment and fatty acid metabolic homeostasis. This study has been approved by the Experimental Animal Ethics Committee of China Three Gorges University (No. 20190801).

OBJECTIVEThis study simulated clinical implant surgery using squeeze technique by establishing a rational animal model. To measure the morphologic parameters in order to observe the agglutination of the cancellous bone after being compressed. The study wanted to get some favourable support of implant squeeze technique in bone healing and obtaining primary stabilities of implants, so as to provide some reasonable and valid guidance in the application and improvement of implant squeeze technique.

METHODSThe cancellous bone in condyles of femur of dog had been chosen as the experimental position, prepared the implant holes using squeeze technique according to different compressing extent (0, 0.6, 1.2, 2.0 mm), and then inserted the corresponding implants into them. After different healing periods (1, 2, 4, 12 weeks), the mongrel dogs were sacrificed and the hard tissue slices were made and then the morphologic parameters were measured.

RESULTSThe morphologic parameter of the bone tissue shows that after being compressed, the trabecular bone thickness was almost constant, while the trabecular bone number increased, and the trabecular bone spacing decreased, obviously in the 1-week and 2-week groups. The differences went down gradually along with the extend of healing time.

CONCLUSIONThe clinical implant surgery using squeeze technique can improve peri-implant bone density so it is good for obtaining primary stabilities of implants.

Agglutination ; Animals ; Bone Density ; Bone and Bones ; Dental Implants ; Dogs ; Osseointegration ; Prostheses and Implants ; Wound Healing

OBJECTIVETo evaluate the antimutagenicity of propolis in vivo and in vitro.

METHODSSalmonella typhimurium strains TA98 and TA100 were used as a test model in vitro against a direct mutagen DMC and an indirect mutagen 2AF with or without S9 mix, and MN formation of mice bone marrow cell and CAs induction of mice testicle cell were applied as a test model in vivo against two mutagens CP and MMC.

RESULTSThe present study clearly demonstrated that propolis could inhibit mutagenicity of both DMC and 2AF directly in a dose-dependent manner, and significant antimutagenic effects (P < 0.05) were obtained in TA98 strain at 2000 and 3000 microg/plate. It also could inhibit mutagenicity of both DMC and 2AF to TA98 strain in a dose-dependent manner, with significant antimutagenic effects (P < 0.05) appeared at 1000, 2000, and 3000 microg/plate. The results of antimutagenicity test in vivo revealed that propolis could inhibit MN formation significantly (P < 0.05) at the doses of 45.0 and 135.0 mg/kg b. w., and decrease the frequency of chromosome aberrants and chromosome aberrant cells significantly (P < 0.05) only at the dose of 135.0 mg/kg b. w.

CONCLUSIONThe propolis is a good inhibitor for mutagencity of DMC and 2AF in vitro, as well as for CP and MMC in vivo.

Air Pollutants ; toxicity ; Animals ; Antimutagenic Agents ; pharmacology ; Bone Marrow Cells ; drug effects ; Chromosome Aberrations ; drug effects ; Dose-Response Relationship, Drug ; Male ; Mice ; Mutagenicity Tests ; Mutagens ; toxicity ; Propolis ; pharmacology ; Salmonella typhimurium ; drug effects ; genetics ; Testis ; cytology ; drug effects

OBJECTIVETo study the strengthening of fluorophlogopite-based dental machinable ceramic through developing a new type of calcium-mica-based ceramic.

METHODSBased on the analysis of the crystal structure of the fluorophlogopite ceramic of Dicor MGC, the structure of a new type of calcium-mica-based ceramic was designed and the corresponding composition of the new material was experimented. And the new glass-ceramic was obtained through the treatment of glass preparation and nucleation. Then crystal content of the glass ceramic was analyzed by X-ray diffraction analysis (XRD) and the 3-point bending strength of the new ceramic was recorded.

RESULTSA new type of calcium-mica-based glass ceramic was developed and had the 3-point bending strength of (210.2 +/- 14.7) MPa. Compared with the strength of fluorophlogopite-based dental machinable ceramic, such as Dicor MGC, which was reported in the range of 150 approximately 180 MPa within inclusive studies, the higher strength of the new ceramic was recorded.

CONCLUSIONSThe fluorophlogopite-based dental machinable ceramic could be reinforced through internal strengthening.

Aluminum Silicates ; chemistry ; Dental Materials ; Dental Porcelain ; chemistry ; Materials Testing ; Tensile Strength

Objective To know the effect of nanometer titanium dioxide (nano -TiO2 )on the oxidative stress in zebrafish. Methods A total of 64 zebrafishes were randomly divided into 4 groups with different concentrations of nano -TiO2 exposure,which including control group,low dose group (2 mg/L),moderate dose group (8 mg/L)and high dose group (32 mg/L).The content of MDA,the activities of SOD and ATPase in gill,liver and muscle were measured in 1 0 zebrafishes in each group after 30 d exposure.Histopathological changes of gill,liver and muscle in the remainder fishes were observed.Results The content of Malondialdehyde (MDA)in the fish gill of the moderate and high dose group were significantly higher than that of the control group (P <0.05).The activity of Superoxide dismutase (SOD)in the fish gill of the high dose group was significantly increased compared with the control group (P <0.05).The activities of Adenosine triphosphatase(ATPase)in the fish gill of the moderate and high dose group were significantly decreased compared with the control group (P <0.05).The changes on the content of MDA and the activities of SOD in the fish liver were similar with that in the fish gill after nano -TiO2 exposure,while there was no significant change in the activity of ATPase of the fish liver.And no significant changes were observed in the content of MDA,the activities of SOD and ATPase in the fish muscle (P >0.05 ). Histopathological observation showed infiltration by inflammatory cells, swelling and degeneration, vacuolation and necrosis in zebrafish gill and liver with different degrees.Conclusion Nano -TiO2 exposure could induce the oxidative stress in zebrafish,causing pathological changes in the fish gill and liver.

Objective To explore the influence of 30 day feeding test of health food containing caffeine on the physiological state of SD rat.Methods SD rats were randomly divided into five groups according weight :three health food containing caffeine(1.64,3.28,6.57 g/kg),one health food containing reduced caffeine (6.48 g/kg)and the control group. Haematological and biochemical parameters were measured at the end of experiment,and main organ tissue analysis was also performed.Results Weight at the end of 4 week,weight after absolute diet,total weight increased,and total food consumption and food consumption at the end of 4 week in 6.57 g/kg groups of male rats were decreased compared with the control group (P <0.05 ).Conclusion In comparison with 6.48 g/kg group,all those changes may be caused by overhigh caffeine in 6.48 g/kg group.