OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

Purpose:To study the efficacy and the side effect of morphine sulphate controlled release tablets by rectal administration in the treatment of cancer pain. Methods:40 patients with cancer pain were randomly divided into two groups. The rectal administration of morphine sulphate controlled release tablet (30 mg,q12h) was given in group Ⅰ, oral in group Ⅱ. The effect were compared. Results:The analgesic effect was good and no significant difference was found in the 2 groups. Conclusions:The rectal adminstration of morphine sulphate controlled release tablets may be used successfully to provided relief of cancer pain.

Objective To investigate the diagnostic value of volume imaging for breast assessment (VIBRANT) dynamic enhanced MR for benign and malignant breast mass.Methods Ninety-eight patients with breast mass underwent both bilateral molybdenum target radiography and VIBRANT dynamic enhanced MR examination.The sensitivity,specificity,positive predictive value,negative predictive value and diagnosis accuracy between two methods were compared.Results There were 113 lesions including 65 lesions in 57 malignant cases and 48 lesions in 41 benign cases.The sensitivity,specificity,positive predictive value and negative predictive value of molybdenum target radiography were 67.69％ (44/65),68.75％ (33/48),74.58％ (44/59) and 61.11％ (33/54),respectively.The values of VIBRANT dynamic enhanced MR were 84.62％ ( 55/65 ),89.58％ (43/48),91.67％ (55/60) and 81.13％ (43/53),respectively.The difference of the diagnosis accuracy between two methods had statistical significance (P ＜0.05).Conclusion The diagnostic value of VIBRANT dynamic enhanced MR for benign and malignant breast mass is higher than molybdenum target radiography.

Objective: To investigate whether the signaling of PI3K/Akt is involved in epithelial-mesenchymal transdifferentiation (EMT) of human kidney tubular epithelial cell (HKC) promoted by transforming growth factor (TGF)-?_1. Methods: the HKC was cultured on plastic plates in the presence or absence of recombinant human TGF-?_1 and PI3K inhibitor wortmannin. The expression of total- and phosphor-Akt and ?-SMA was assessed at different time point by Western blot. Results: In HKC, basic level of p-Akt, ?-SMA expression was found in the normal condition. Profound increase of p-Akt was seen at 0.5 h after induction of 10 ng/ml TGF-?_1 in HKC.?-SMA increased markedly at 48 h after the treatment of TGF-?_1. Adding PI3K inhibitor wortmannin (10 nmol/L) largely abrogated the effect of TGF-?_1. Wortmannin was shown to down-regulate ?-SMA and p-Akt expression in response to TGF-?_1. Conlusion: PI3K/Akt is a downstream mediator in EMT of HKC promoted by TGF-?_1, and PI3K inhibitor wortmannin can significantly inhibit EMT induced by TGF-?_1.

The authors first expound the connotations and origin of the legal person management structure and then discuss the following issues: the dual relation of agency by agreement characteristic of medical institutions, the need to take management structure as the priority of hospital reform in delegating powers to lower levels, the primary strategies and major means of the legal person management structure of corporations, and questions related to the management structure of the medical industry abroad: who should become the agent of the patient, how to unify the interests of the agent and the consignor by means of reasonable remuneration designs, etc. Finally the authors give an account of the inspiration that can be drawn from the legal person management structure to the public hospital property right system reform in China.

Objective To investigate the effect of activated PI3-K on epithelial-mesenchymal transdifferentiation of HKCs induced by transforming growth factor?1 (TGF-?1). Methods The human kidney proximal tubular epithelial cells (HKCs) cultured on plastic plates were divided into following groups: cultured with free serum medium (FSM); culture in the different concentrations of TGF-?1; cultured in the presence of recombinant human TGF-?1 and PI3-K inhibitor Wortmannin. The expression of total and phosphor-Akt (t-Akt and p-Akt) was assessed at different time points by Western blot,? smooth muscle actin(?-SMA) and E-cadherin were detected by Western blot. Results A marked increase in p-Akt was seen in HKC at 1h after being induced by TGF-?1, and its protein level was enhanced in a TGF-?1 concentration-dependent manner. Protein level of ?-SMA was increased markedly at 48 hours after the treatment of TGF-?1, but protein level of E-cadherin was decreased 48 hours after treatment of TGF-?1. Addition of PI3-K inhibitor Wortmannin (10nmol/L) largely abrogated the effect of TGF-?1. Wortmannin was showed to down-regulate ?-SMA and p-Akt expression and in response to TGF-?1 and up-regulate E-cadherin expression. Conclusion PI3-K was activated in epithelial-mesenchymal trans-differentiation of HKCs promoted by TGF-?1, and PI3-K inhibitor Wortmannin can significantly inhibit TGF-?1 induction of EMT.

BACKGROUND: The renal transplanted recipients were in poor immunosuppressive state. Compared to common person, the bladder transitional carcinoma in recipients was aggressive and easy to recurrence. Looking for a more effective therapy method to decrease the recurrence of recipients' bladder transitional carcinoma is the hot and difficult problem in clinical study.OBJECTIVE: To analyze the efficacy and safety of submucosal injection epirubicin following transurethral resection of bladder tumor (TUR-Bt) to treat the recurrence of bladder transitional cell carcinoma in renal transplantation recipients.METHODS: Totally 9 renal transplantation recipients with transitional cell carcinoma of bladder were retrospectively studied. The patients' periods without cancer, the frequency of recurrence within one year, the rates of side effect, the changes of tumor grading following recurrence and allograff function were recorded when the routine method and submucosal injection epirubicin following TUR-Bt were used in different period respectively.RESULTS AND CONCLUSION: Submucosal injection epirubicin following transurethral resection of bladder tumor was safe and effective to treat bladder transitional cell carcinoma recurrence in renal transplantation recipients. Compared to the routine perfusion, periods without cancer and the frequency of recurrence within 1 year were significantly decreased, which can elevate recipients life quality and long-term survival rates.

As a secreted glycoprotein,Dickkopf-1 (DKK1) is closely related to tumor metastasis,and it is highly or lowly expressed in tumor.The high expression of DKK1 promotes the metastasis of a variety of malignant tumors including liver cancer,gastric cancer,non-small cell lung cancer and breast cancer.Recent studies have found that DKK1 is inextricably bound up with the latent metastasis of tumor.

Objective To investigate the effect of atorvastatin on the curative effect and cardiovascular events in patients with unstable angina pectoris.Methods128 patients with UAP were enrolled in Jinhua Central Hospital from August 2014 to August 2016, and 128 patients with UAP were randomly divided into observation group and control group.All the patients were given low salt and low fat diet.Two groups of patients were anti-platelet aggregation, blood pressure, lipid-lowering and other conventional treatment, observation group on the basis of simultaneous atorvastatin calcium 40mg/d, oral, once every month.The levels of TC, TG and LDL-C, HDL-C and the incidence of cardiovascular events were compared between the two groups before and after treatment.ResultsThere was no case of cardiac death in the observation group and the control group.The incidence of cardiovascular events in the observation group was 9.38%, which was significantly different from that in the control group (P<0.05).The levels of TC, TC and LDL-C in the observation group were significantly higher than those before treatment and control group, respectively.The clinical effect of the observation group was significantly better than that of the control group, the difference was significant (P<0.05).ConclusionAtorvastatin can significantly improve the efficacy of unstable angina treatment, and reduce blood lipid levels, reducing the incidence of cardiovascular events, improve the prognosis.

Objective To investigate the expression of thyrotrophin-releasing hormone receptor type-2(TRH-R2) in rat testis during postnatal development,and to discuss its function in the regulation of reproduction development. Methods Western blotting and the immunohistochemical ABC method were used to detect the expression and location of TRH-R2 in testes of 8,15,20,35,60 and 90-day-old rats. And the immunohistochemical results were analyzed with the image analysis system and statistical method. Results Western blot showed that TRH-R2 was expressed in 15- to 90-day-old rat testis. As for the immunohistochemical ABC method,we found that TRH-R2 firstly appeared in 8-day-old rat testes. In all the following stages we also detected the immunochemical positive cells in testes. TRH-R2 was distributed in the interstitial cells between seminiferous tubules and positive substance was expressed in the cell membrane and cytoplasm of Leydig cells.The results of image analyses quantitation revealed that the intensity of TRH-R2 increased progressively with the postnatal development of rats.The peak was in 35-day-old.Then at the following stages the expression of TRH-R2 maintained stable.Conclusion TRH-R2 is expressed in rat testis during its postnatal development and locates in the Leydig cells,and the TRH-R2 shows development-dependent expression in testicular tissue.