ve To study the growth curve of Alexandrium tamarense and the toxicity of paralytic shelfish poisoning(PSP) selected in different growth phases, as well as to quantitatively analyze the toxins and compare the different efficiency of breaking cell wall and their virtues and defects between freezing-thawing method and supersonic method, and to provide theoretical basis for the selection of the methods for extraction of PSP. Methods Cells were collected by suction filter, cell wall was broken by freezing-thawing method and supersonic method, the toxicities of Alexandrium tamarense among different growth phases were detected and compared. Results The growth of Alexandrium tamarense typically showed three phases which included a lag phase(0~9 days), an exponential growth phase(10~18 days), and a stationary phase(19~22 days) . The maximum toxicity of cells in the stationary phase appeared, especially in day 21 when the population of cells reached to 9.43?10-6 MU/cell, although the cells in the exponential phase grew fastest. Toxicity of the cells in the lag phase broken by freezing-thawing method was more stronger than that by supersonic method, that meant the toxicity of cells was lowered after the supersonic treatment. In additional, the filtering method for collecting cells presented a lower recovery rate of average 69.0% . Conclusion As for the comparing of freezing-thawing method and supersonic method, it was found that, when cells were in the same phase, the supersonic method had remarkable advantages, but it also had the disadvantage of lowering the tox-icity of cells. When in the same condition of growth and method of fragmentation, cells in the lag phase were broken most easily, and cells in the stationary phase were more difficult to be broken than those in the other two phases. Toxicity of cells was larger and larger with the time of incubation. The toxicity of cells in the lag phase had reached a higher intensity, although toxicities of cells in the next two phases would increase a little.

A questionnaire is carried out in order to understand the status quo of life view among medical students in our college.The analysis of the data shows there are deviations in treating death,significance of life,life value,etc.Therefore,we conclude that it's imminent to strengthen the life-education for medical students.

ObjectiveTo evaluate the efficacy of the high-flux dialysis on maintaining hemodialysis patients. Methods60 hemodialysis patients were randomly divided into the ordinary PMMA membrane dialyzer group,the ordinary polysulfone membrane dialyzer group and the high-flux dialyzer group with 20 patients in each group.Before and after the single hemodialysis,the removing effect of serum β2- microglobulin (β2-MG)in the 3 kinds of dialyzer,and cholesterol,triglyceride,low density lipoprotein changes after 6 months were compared. ResultsThe ordinary PMMA membrane dialyzer group and high-flux dialyzer group can reduce the concentration of β2-MG,while the high-flux dialyzer group showed more significant effect.On the aspect of lipid metabolism,in the high-flux dialyzer group,serum total cholesterol,triglycerides and LDL levels decreased significantly,while the ordinary dialysis group had no significant change. ConclusionsThe high-flux dialysis can improve the dialysis efficiency,life quality and reduce the complications of patients.

Objective To investigate the incidence of the pusher syndrome in stroke patients, and the relationship between the syndrome and the neuropsychological symptoms and location of brain lesion, and to investigate the mechanism and the physiotherapy intervention of pusher syndrome. Methods Thirty nine patients(25 male, 14 female, 62.5?9.4 years old) with pusher syndrome were examined, 91 patients(57 male,34 female, 58.4?11.6 years old) without ipsilateral pushing served as control. The lesion areas, neuropsychological syndrome and the Barthel Index(BI) were recorded, and the physiotherapy were administered in the patients. Results The incidence of pusher syndrome was 30% in the patients studied, corresponding to 17% of the total number of stroke patients in the study period. The percentage of pusher syndrome occurrence was higher in the patients with right side lesion than those with left side lesion ( P

OBJECTIVETo elucidate the necessary and research of accelerating basic research of Chinese standard pieces as standard materials.

METHODAccording to over 10 years accumulated experience and be keenly aware of the author, the evaluation method of standardized processing technology and Chinese pieces quality, aimed at consummated the standard material of the quality evaluation of Chinese herbal pieces at the current situation, and inaccordance with the need of improving quality standard system of Chinese herbal pieces, illustrate the necessity of accelerating basic research of Chinese standard pieces as standard materials; from the technical specification for collecting and processing of raw materials, and the technical specification, homogenized sample, packaging, storage and etc., for processing of candidate standard pieces, determine the methods and steps of technical specifications for standard pieces as the standard substance, determine the methods and steps of technical specifications for standard pieces as the standard substance.

RESULT AND CONCLUSIONTo speed up the basic research of standard of Chinese medicine pieces as of standard material is very necessary. The research objective is to specificate the processing technical for a number of standard pieces, to identify technical specifications and to ascertain the guiding principle and technical specification of decoction pieces as standard substance. This research will provide basic scientific data relevant national departments to apply for the accreditation of the standard substance.

Objective:To observe therapeutic effect and safety of six‐month atrovastatin sequential treatment on pa‐tients with acute coronary syndrome (ACS) undergoing PCI .Methods :A total of 280 ACS patients were consecu‐tively enrolled ,randomly and equally divided into sequential treatment group (received atorvastatin 80mg 2d before PCI operation ,then 40mg/d after operation ,20mg/d a month later ) and routine treatment group (no taking atorv‐astatin before PCI operation ,then 20mg/d after operation and as a maintenance dose ) .Both groups were observed for six months ,therapeutic effect and safety were compared between two groups .Results:Compared with before PCI ,there were significant rise in levels of creatine kinase isoenzyme (CK‐MB) and cardiac troponin I (cTnI) after PCI 18h~24h in two groups ,and those of sequential treatment group were significantly lower than those in routine treatment group [CK‐MB:(2.72 ± 0.52) μg/L vs .(6.04 ± 0.51) μg/L ,cTnI : (0.28 ± 0.13) μg/L vs .(0.42 ± 0.16) μg/L , P < 0.01 both ];Compared with routine treatment group , the high sensitive C reactive protein (hsCRP) level significantly reduced in sequential treatment group after PCI 2d ,and continued until post‐operation six months [ (2.34 ± 0.51) mg/L vs .(1.49 ± 0.78) mg/L ,P=0.001];the blood lipids levels (except level of high density lipoprotein cholesterol significantly rose ) in sequential treatment group were significant lower than those of routine treatment group , P<0.01 all;there were no significant difference in percentage of transaminase level high‐er than normal three times and incidence rates of major adverse cardiovascular events (MACE) between two groups , P>0.05 all .Conclusion:Preoperative 80mg atrovastatin combined postoperative 40mg atrovastatin treatment can significantly reduce inflammatory reaction ,improve blood lipids ;compared with routine dose ,it doesn't significant‐ly increase adverse drug reactions ,so it's safe and effective .

Child, Preschool ; Diagnostic Errors ; Female ; Humans ; Lung Diseases, Parasitic ; diagnosis ; Pericardial Effusion ; diagnosis ; parasitology ; Pleural Effusion ; diagnosis ; parasitology ; Trematode Infections ; diagnosis

Bilingual teaching is the important teaching method of medical colleges to keep up with the international progress. This article summarizes the experience of bilingual teaching practice in medical imaging, makes a deep analysis on the problems that need to be solved promptly, and suggests some new ideas about the selection of textbook, preparation of teaching slides and teaching mode.

Objective To compare dynamic hip screw (DHS),InterTan and proximal femoral nail antirotation-Ⅱ (PFNA-Ⅱ) in the treatment of femoral intertrochanteric fracture in terms of their effects on postoperative hidden blood loss (HBL) and deep venous thrombosis (DVT).Methods We retrospectively analyzed the 133 patients with femoral intertrochanteric fracture who had been treated in our hospital from November 2011 to November 2015.Of them,42 received DHS treatment,including 22 males and 20 females;43 underwent InterTan fixation,including 21 males and 22 females;48 had PFNA-]Ⅱtreatment,including 25 males and 23 females.At preoperation,1,3 and 7 days postoperation,all of them had tests of hemoglobin (Hb),hematocrit value (Hct),blood platelet (PLT),thrombin time (TT),activated partial thromboplastin time (APTT),prothrombin time (PT),human fibrinogen (FIB) and D-dimer.DVT was detected using color Doppler ultrasound at 7 days postoperation.Results At 1,3 and 7 days postoperation,the DHS patients had the smallest values of Hb,Hct,TT,PT and APTT while the PFNA-Ⅱ patients the largest.The DHS patients had the largest values of intraoperative bleeding,PLT,FIB and D-dimer while the PFNA-Ⅱ patients the smallest.There were significant differences between the 3 groups in all the indexes (P ＜ 0.05) except in TT at 7 days postoperation and APTT at 1 and 7 days postoperation between the InterTan and PFNA-Ⅱ groups (P ＞ 0.05).The incidence of DVT in the InterTan group (2.3％,1/43) and in the PFNA-Ⅱ group (0) was significantly lower than in the DHS group(7.1％,3/42) (P ＜ 0.05).Conclusion In the treatment of femoral intertrochanteric fracture,PFNA-Ⅱ may be superior to InterTan and DHS in reducing postoperative HBL and control of DVT.

Objective To compare the efficacy of intra?articular dexmedetomidine or morphine mixed with ropivacaine for postoperative analgesia after arthroscopic knee surgery?Methods Sixty ASA physical status I or II patients scheduled for elective arthroscopic knee surgery were randomly assigned into 3 equal groups using a random number table?The group R received 0?25% ropivacaine, and the group RD received 1 μg/kg dexmedetomidine and 0?25% ropivacaine,and the group RM received 2 mg morphine and 0?25% ropivacaine intraarticularly in a total volume of 20 ml?Visual analogue scales( VAS) scores when the patients actively flexed the operated knee to 90° were recorded at 2, 4, 8, 12, 18 and 24 h after surgery?The analgesic duration and consumption of fentanyl at 24 h after surgery were recorded?The complications such as bradycardia,hypotension, nausea,vomit,cutaneous pruritus and uroschesis were followed up?Results At 8 and 12 h after surgery,VAS scores were significantly lower in group RD ( ( 3?23 ± 0?45 ) points, ( 3?18 ± 0?47 ) points ) and RM ( ( 3?20 ±0?46) points,(3?13±0?45) points) than in group R((4?01±0?74) points,(3?93±0?71) points),and at 18 and 24 h after surgery,they were significantly higher in group R((3?85±0?62) points,(3?72±0?57) points) and RD((3?83±0?57) points,(3?71±0?55) points) than in group RM((3?07±0?43) points,(3?02±0?41) points),and there was significant difference(F between groups=124?65,P=0?021,F inner grouP=11?65,P=0?004,F across groups=67?65,P=0?024)?The analgesic duration was significantly different in three groups (F=45?78,P<0?001),and in group RD((668?3±133?4) min) and RM((1 412?8±275?67) min) was significantly longer than in group R(402?3±81?5) min,P<0?05),and the group RM was longer than the group RD( P<0?05)?The consumption of fentanyl at 24 h after surgery was significantly different in three groups ( F=34?47,P<0?001),and it was significantly lower in group RD((32?6±7?3) μg) and RM((12?8±3?7) μg) than in group R((151?3±28?5) μg,P<0?05),and the group RM was lower than the group RD(P<0?05)?No significant side effects were found?Conclusion Intra?articular dexmedetomidine or morphine all can improve the efficacy of ropivacaine for postoperative analgesia after arthroscopic knee surgery,while morphine is superior to dexmedetomidine.