Eukaryotic DNA element called Matrix Attachment Regions (MARs) can function on regulating the structure and activity of chromosome. Traditional quantitation in vitro and indirect functional analysis can not always reflect MAR-involved physiological state. In order to study transcription regulation and make a try in methodism,? 1-antitrypsin MAR (?1-AT MAR) is cloned and incorporated into pEGFP-C1 vector. Non-MAR-containing and MAR-containing plasmids were then transfected into HEK-293 cells with LipofectamineTM 2000 respectively. Positive cell clones were assayed after 20 days of selection by G418. Semiquantitative RT-PCR and fluorescence microscope analysis show that this MAR has a positive effect on modulating nearby gene expression. Further, co-localization with newly CMV promoter and RNA polymeraseⅡ(RNAPⅡ) was detected by chromatin immunoprecipitation (ChIP), The PCR result demonstrates that more RNAPⅡwas recruited to the CMV promoter to initiate transcription in presence of MAR. ChIP can be used to confirm the MAR-mediated transcriptional activation and provide more reliable information than RT-PCR in real time. The technology is also providing a platform for our research in gene expression regulation.

Child ; Hodgkin Disease ; therapy ; Humans

Objective To investigate the circadian blood pressure(BP)rhythm in hypertensive patients with chronic renal insufficiency.Methods Circadian blood pressure(BP)rhythm was studied by ambulatory 24-h monitoring among hypertensive(n=25,group B)patients with stable progression of chronic renal insufficiency,and among patients with essential hypertension(n=28,group A)without renal disease.Results ①The 24-h diastolic BP and nocturnal systolic and diastolic BP of group B were significantly higher than those of group A[(80.9±13.4)mmHg vs[70.3±15.6)mm Hg,(160.2±17.8)mm Hg vs(140.3+25.9)mm Hg,(82.6±16.1)mm Hg vs(68.8±20.2)mm Hg,P＜0.05)].②Blooa pressure variability(BPV)of 24 hour systolic and diastolic BP of group B was higher than group A[(13.5±3.9)mm Hg vs(11.3±2.1)mm Hg and(9.2±1.2)mm Hg vs(8.3±1.8)mm Hg,P＜0.05).BPV of nocturnal systolic and diastolic BP in group B was signitleantly higher than group A [(14.9±3.3)mm Hg vs(9.3±2.1)mm Hg and (9.7±2.4)mm Hg vs(8.0±2.2)mm Hg,P＜0.01)].③The 24-h profile of BP of group A showed more non-dipper according for 64.3%(18/28),reverse-dipper according for 10.7%(3/28),but which was 40.O%(10/25)and 48.0%(12/25).Conclusion The renal patients show a disturbance in the 24-h profile of BP,higher indexes of noetttrnal BP and a greater nocturnal variability,which may be important factor for event of cardiovascular disease and renal insufficiency.

Gaucher Disease ; Humans

Child ; Humans ; Lymphoma, Follicular ; diagnosis ; therapy

Child ; Humans ; Tuberculosis, Renal

Objective To study the clinical effect of homemade rapamycin-eluting stent on acute myocardial infarction(AMI).Methods A total of 116 AMI patients who received emergency PCI were divided into two groups,the Cypher group(n=52)and the Partner group(n=64).There was no obvious difference in ages,female ratio,smoking,hypertension,hyperlipidema and diabetes between the two groups.The MACE and the restenosis were the targets we wanted to observe.Results The emergency PCI were all successful.Fifty-five Cypher stents and sixty-four Partner stents were planted.The MACE was 0 during in-hospital and the follow-up.Eleven patients in Cypher group(21.15%)and eighteen patients in Partner group(28.13%)underwent coronary angiography re-examination at the 6 months' post-stenting,the restenosis both being 0.Conclusion The homemade rapamycin-eluting stent is safe and effective in AMI.

Objective To investigate the clinical features of the childhood chronic myelogenous leukemia (CCML), including the pathogenesis, incidence, clinical characteristics, diagnostic criterion, prognostic significance and the treatment strategies, etc. Method The data of 148 cases of CCML were comprehensively reviewed and analyzed, and international and domestic literature in the last two decades was reviewed. Results The CCML was found to be rare with unknown etiology, and was an acquired malignant disease of clonal proliferation of hematopoietic stem cells in children. The disease included two clinical types: adult CCML and juvenile CCML. 72.3% of CCML patients were diagnosed as the adult CCML. The clinical feature of CCML consisted of fatigue, low fever, anemia, hepatomegaly, splenomegaly, and lymphadenopathy. The laboratory findings of a typical CCML patient comprised of peripheral blood leukocytosis, basophilia and eosinophilia, myeloid differentiation in different stages, and increased megakaryocytes. The immunohistochemical features of the CCML consisted of highly positive MPO and CD68, significant lowering of neutrophil alkaline phosphatase (NAP), positive for Philadelphia chromosome (Ph) or chimeric BCR/ABL gene, etc. But in most cases of juvenile CCML, the Philadelphia chromosome could not be detected. The Gleevec therapy and hematopoietic stem cell transplantation (HSCT) might give better treatment result for CCML than the traditional therapy. Conclusions CCML has its characteristic clinical feature. The key of good therapeutic result is early diagnosis and treatment. The optimal therapy for CCML is Gleevec regime and HSCT.

Objective To explore the clinical effect of hydrochloride phenazopyridine on relieving the pain after cystoscopy. Methods 60 male patients undergoing cystoscopy were divided into observation group and control group according to the block randomization method with 30 patients in each group. 1 h after cystoscopy, the observation group was given orally hydrochloride phenazopyridine 0.2 g, 3 times a day for 0.6 g totally. The control group was not treated with oral medication for pain. The pain degree was evaluated with Numerical Rating Scale (NRS), and the side effects were recorded. Results There was no significant difference in NRS score immediately after cystoscopy between 2 groups (P=0.725). The NRS score was lower in the observation group than in the control group 24 h after cystoscopy (P=0.002).Conclusion Hydrochloride phenazopyridine can effectively relieve the postoperative pain of cystoscopy.

A method for the analysis of five types of triphenylmethane dye residues in water was developed by using solid phase extraction ( SPE) combined with ultra performance liquid chromatography coupled to triple quadrupole linear ion trap mass spectrometry ( UPLC-MS/MS/MS ) . The water samples were extracted and cleaned with mixed-mode cation exchange solid-phase extraction cartridges ( MCX) . The UPLC separation was performed on a C18 column with a linear gradient elution program of acetonitrile and 5 mmol/L aqueous ammonium acetate ( containing 0. 02% formic acid) as mobile phase. Triphenylmethane dye residues were analyzed by mass spectrometry under an electrospray ionization interface ( ESI ) in positive ion mode with MRM-IDA-EPI mode. The calibration curves of five types of triphenylmethane dyes were linear in the range of 0. 02-20 μg/L, and the correlation coefficients were not less than 0. 998. The recoveries at spiked levels of 5 , 10 and 20 μg/L were in the range of 70 . 9%-101%, and the RSDs were 3 . 4%-11 . 9% ( n=6 ) . The limit of detection and quantification were 0. 42-2. 18 ng/L and 1. 68-8. 73 ng/L, respectively. The method was performed as sensitive and accurate, and also suitable for simultaneous determination of five types of triphenylmethane dye residues in water.