Objective To evaluate the clinical efficacy and safety of two glycolic acid-containing liquid preparations for the treatment of facial acne vulgaris.Methods Sixty patients with mild to moderate acne vulgaris were included in a randomized,single-blinded,split-face,controlled study.The left and right sides of each patient's face were randomly assigned to the test group (treated with one hydroxy acid-containing liquid preparation) and control group (treated with the other hydroxy acid-containing liquid preparation).In total,all the patients were given four times of treatment at week 0,2,6 and 10 with the concentration of hydroxy acid in both preparations being 20％,35％,50％ and 70％,respectively.The number of inflammatory and noninflammatory acne lesions was counted,and the improvement in acne mark,scar and pores was evaluated by a 6-point grading scale before every treatment and 4 weeks after the last treatment.Results At 2,6,10,and 14 weeks after initiation of treatment,the clearance rate for inflammatory lesions was 20.4％,43.3％,66.9％ and 80.2％ respectively in the test group,21.6％,44.2％,68.1％ and 79.8％ respectively in the control group,while that for noninflammatory lesions was 18.5％,34.0％,41.3％ and 59.6％ respectively in the test group,17.8％,31.2％,44.9％ and 57.1％ respectively in the control group.The response rate was 80.0％ in the test group and 78.3％ in the control group at the end of the study.Adverse effects were observed in both groups,including temporary pain,erythema and formation of hoar frost,all of which well responded to symptomatic treatment.There were no significant differences in clinical efficacy or adverse effects between the two groups (both P ＞ 0.05).Conclusion Both of the hydroxy acid-containing preparations are effective and safe for mild to moderate acne vulgaris.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; epidemiology ; Young Adult

Objective:To study the effects of Shixinyatong buccal tablets(SBT)in patients after the extraction of mandibular third molar.Methods:150 cases of patients were divided into 3 groups(n =50).After tooth extraction the patients in SBT,cephalosporin and control groups were given SBT at 0.6 g,4 times per day,cepholosporin 0.5 g,2 times per day and no drug respectively.At the 3rd and 5th day the patients were followed up and their local symptoms were scored.The data were statistically analyzed with SAS 9.0 soft ware.Results:Between SBT and cepholosporin or control group there was no statistical difference in demographic data and impac-tion types of the teeth(P >0.05).The total symptom integral,primary symptom integral,minor symptoms,wound pain,redness, swelling degree and oral odor in SBT group were lower than those in control group(P <0.05)at the 5th day after tooth extraction.Be-tween SBT group and cephalosporin group there was no significant difference(P >0.05)in the total symptom integral,primary symp-tom integral,secondary symptom integral at the 3rd and the 5th day.Conclusion:Shixinyatong buccal tablet is effective in the preven-tion of complications after the extraction of mandibular third molar.

Objective To evaluate the effectiveness and safety of a sodium hyaluronate-containing dressing for skin wound healing after Q-switched laser therapy.Methods Sixty-six patients with facial pigmentary disorders were enrolled in this study.After treatment with Q-switched laser,the patients were randomly and equally divided into three groups to apply a sodium hyaluronate-containing dressing,a thermal spring water-containing facial mask and a distilled water-containing facial mask respectively.The dressing or facial masks were externally applied to the face for 15 minutes once immediately after the laser therapy,then,once every night for 14 consecutive days.Inflammatory responses,such as erythema,edema and burning sensation,were measured semi-quantitatively on day 1,3,7 and 14 separately after the laser therapy.The time for skin wound healing and degree of patients' satisfaction were compared among these groups by chi-square test and rank sum test,respectively.Results The sodium hyaluronate-containing dressing and thermal spring watercontaing facial mask were superior to the distilled water-containing facial mask in the improvement of erythema,edema and burning sensation after the laser therapy (all P ＜ 0.05).Compared with the patients applying the thermal spring water-containg facial mask and those applying the distilled water-containing facial mask,those applying the sodium hyaluronate-containing dressing felt better,with significantly increased skin cleanliness,humidity,smoothness,confort degree and overall satisfaction (all P ＜ 0.05).Conclusion The sodium hyaluronate-containing dressing is effective for improving acute inflammatory responses and increasing skin cleanliness,humidity and smoothness after laser therapy.

Objective To assess the in vitro effect of high intensity narrow-band blue light on bacteria isolated from patients with acne vulgaris (AV). Methods Bacteria were isolated by culture from the acne lesions of 67 patients with mild to moderate AV. Selected bacterial strains were chosen for irradiation with the blue light. The morphology and number of colony formation units (CFUs) as well as the ultrastructure of the bacteria were observed before and after the irradiation. Results Propionibacterium acnes (P. acnes) (32.4%), P. granulosum (6.9%), Staphylococcus epidermidis (S. epidermidis) (35.3%), S. aureus (4.9%), S. heamolyticus (7.8%), S.cohnii (1.0%), Corynebacterium spp. (9.8%), Klebsiella pneumoniae (1.0%) and B group Streptococci (1.0%) were isolated from the lesions. In vitro investigation revealed that the irradiation significantly reduced the number of CFUs of P. acne and S. epidermidis. Conclusion High intensity narrow-band blue light is effective against bacteria isolated from AV lesions.

Objective Obtaining chemical constituents of Angelica apaensis collected in Qiaojia County of Yunnan Province and detecting their anti-tumor activities in vitro. Methods The MeOH extract of was repeatedly subjected to column chromatography over silica gel, Sephadex LH-20 and/or RP-18 and semipreparative HPLC. Their anti-tumor activities in vitro were measured by MTT colorimetric assay. Results Eight compounds were isolated from the roots of this plant. Conclusion The major type of chemical constituents of in Yunnan is furocoumarin. These separated compounds showed weak anti-tumor activity in vitro.

Since the polyjuice potion ingredient is complex, we need to develop an analysis method with well separation and high stability to perform qualitative analysis. After dividing chemical components of Mahuang Decoction into fat-soluble and water-soluble constituents by gradient extraction, GC-MS was used to analyze the chemical components of the ethyl acetate extraction. The results showed that forty compounds had been identified by NIST MS search 2.0 standard mass spectrometry Library and literatures. Next, UPLC-Q-TOF-MS was applied to idendify the chemical components of the water extraction. The results showed that thirty-nine compounds had been identified by MZmine-2.9.1, Isotope Pattern, fragmentation regularity of mass spectrometry and literatures. This experiment will provide evidences for elucidation of the effective substance in Mahuang decoction and can be used as a simple, shortcut method for analysis and identification for the polyjuice potion.
Chromatography, High Pressure Liquid ; methods ; Ephedra ; chemistry ; Ephedra sinica ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods

Objective To investigate the effects of heart displacement on hemodynamics during off-pump coronary artery bypass grafting (OP-CABG) while the sites for anastomosis were being exposed. Methods Forty-seven patients of both sexes (36 male, 11 female) aged 50-82 years undergoing OP-CABG were enrolled in the study. Preoperative cardiac function was assessed : class Ⅱ in 22 patients; Ⅲ in 23 and Ⅳ in 2 according to NYHA classification.The mean ejection fraction was 0.55?0.14 before surgery.They received on average 3.2 grafts. Premedication consisted of intramuscular morphine 10 mg, midazolam 3-5 mg and scopolamine 0.3 mg.Before induction of anesthesia ECG and SpO2 were monitored and radial artery was cannulated for continuous direct BP monitoring. Anesthesia was induced with midazolam 0.1 nig?kg-1 , fentanyl 4?g?kg-1 and pancuronium 0.1 mg?g-1 iv.The patients were mechanically ventilated after tracheal intubation and PETCO2 was maintained at about 40 mm Hg. Anesthesia was maintained with isoflurane and 50%-60% N2O in O2 and intermittent intravenous boluses of fentanyl and pancuronium. Swan-Ganz catheter which can continuously monitor mixed venous blood O2 saturation (SvO2) was placed in pulmonary artery via right internal jugular vein. SvO2, cardiac output (CO), BP, pulmonary arterial pressure (PAP) and HR were continuously monitored. Right atrial pressure (RAP) and PAWP were measured intermittently. Cardiac index (CI),stroke index (SI),systemic vascular resistance index (SVRI),PVRI, left and right ventricular work index (LVWI,RVWI) and left and right ventricular stroke work index (LVSWI,RVSWI) were calculated. The hemodynamic parameters were recorded after induction of anesthesia before surgery (T1,baseline),before heart displacement (T2), while anastomosis to anterior descending branch was being made (T3), while anastonosis to right coronary artery or posterior descending branch (T4) and to left circumflex artery or diagonal branch (T5) was being made, after normal heart position was resumed (T6) and at the end of operation (T7). Results While anastomosis to the anterior descending branch was being made (T3) SI and LVSWI significantly decreased as compared with the baseline (P

OBJECTIVETo study the pathogen and characteristics of viral diarrhea in children in Changchun area.

METHODS460 stools specimens were collected from children with acute diarrhea cured in the childrens, hospital of Changchun in 2010. Rotavirus were detected by ELISA, caliceverus and astrovirus were detected by reverse transcription-polymerase chain reactions (RT-PCR), adenovirus were detected by polymerase chain reactions (PCR).

RESULTSA total of 460 specimens were detected. The detection rate of rotavirus, caliceverus, astrovious, adenovious respectively is 35.22%, 20.43%, 9.78%, 3.70%, the detectablerate of mixed infection is 7.61%, children under 2 years old were the major patient. The main genotypes of the virus: rotavirus (G3P[8]), caliceverus (GII-4), astrovious (type I), adenovious (Ad41).

CONCLUSIONRotavirus is the main pathogen in Changchun. Followed by caliceverus, astrovious, adenovious.

Adenoviruses, Human ; isolation & purification ; Caliciviridae ; isolation & purification ; Child, Preschool ; China ; epidemiology ; Diarrhea ; epidemiology ; etiology ; Female ; Humans ; Infant ; Male ; Mamastrovirus ; isolation & purification ; Rotavirus ; isolation & purification ; Virus Diseases ; epidemiology ; etiology

Background Cellular autophagy is a non-apoptosis death form of tumor tissue.Research determined that arsenie trioxide (As2O3) leads to apoptosis of tumor cells.But whether As2O3 induce autophagy of SO-Rb50 cells or not is unclear.Objective This study was to assess the effects of As2O3 on autophagy of SO-Rb50 cells.Methods As2O3 with the concentration of 0,0.5,1.0,2.0,4.0 μmol/L was used to treat the SO-Rb50 cell line for 48 hours,and the growth and proliferation of SO-Rb50 cells were detected using MTT assay (A570).pGFP-LC3,a marker of autophagy,was constructed to transfer SO-Rb50 cells,and the cells were then divided into RPMI-1640 culture group (untreated group),As2O3 + RPMI-1640 culture group (As2O3 treated group) and rapamycin culture group (positive control group).Autophagy of SO-Rb50 cells was examined by laser confocal microscope and monodansylcadaverine (MDC) influorescence staining,respectively,48 hours following cell culture.Ultrastructural features of autophagy were examined with transmission electron microscope (TEM).The percentage of autophagy positive cells in different concentrations of As2O3 treated groups was calculated with flow cytometer.Results The A570 values of SO-Rb50 cells were 2.194±0.066,1.841 ±0.213,1.035±0.046,0.374±0.042 and 0.167±0.019 in 0,0.5,1.0,2.0,4.0 μmol/L As2O3 treated groups,with a significant difference among these 5 groups(F=547.636,P＜0.05),and those of 0.5,1.0,2.0,4.0 μmol/L As2O3 treated groups were significantly reduced in comparison with untreated group (P =0.000).The positive granular spots for GFP-LC3 chimeric protein were seen to aggregate in autophagic vacuoles in the As2O3 treated group and positive control group,but diffuse cytoplasmic signal for GFP-LC3 was found in the untreated group.Normal ultrastructure of SO-Rb50 cells was exhibited in the untreated group,and many double-membrane-like bound vesicles and autlysosomes were documented in the As2O3 treated group and positive control group under the TEM.A lots of MDC fluorescence granule were found in the As2O3 treated group and positive control group rather than the untreated group.Flow cytometry showed that the percentages of SO-Rb50 cells were 0,15.6％,42.7％,57.9％,79.5％ and 89.0％ in the 0,0.5,1.0,2.0,4.0 μmol/L As2O3 groups and positive control group,respectively,showing a As2O3 concentration-dependent increase.Conclusions As2O3 can induce the autophagy of SO-Rb50 cells and inhibit the proliferation of SO-Rb50 cells.Autophagic response of SO-Rb50 cells appears prior to the nuclear change after exposed to As2O3.The degree of autophagy of SO-Rb50 cells is associated with As2O3 dose.