[Objective]To measure the contents of Nutmeg ether,and its regularity varying with decoction time.[Method]RP-HPLC method was used.The chromatographic conditions were listed below Tigerkin-C18 column(150mm?4.6mm.5?m),amobine phase composed of metranol-0.025mol/L phosphoric acid(70∶30),a flow rate of 1mL ?min-1 and wave lengths was 282nm,column temperature was 25 ℃.[Results]In the range of 0.4?g ~2.0?g,the linear was very well between the content of Nutmeg ether and peak area.[Conclusion] The contents of Nutmeg ether in decoction reached the peak at 2.45min after Nutmeg ether was added in boiling water.

Forty-four mice were used in this experiment. The animal model of septic shock was produced by intraabdomcnal injection of the viable B. coli. The animals were divided into 5 groups. During the course of the development of the septic shock.some of the animals were killed and blood from inferior vena cava was withdrawn for the determination of cAMP, cGMP and cortisol. Tissues from both sides of the adrenal glands, lower pole of the left kidney, left ventricle and right lobe of liver were removed for determination of cAMP and cGMP. The results showed that, during the early stagenof septic shock, the levels of cAMP, cGMP and cortisol were raised, followed by steady lowering of these levels as the shock became more profound.Base on the results of this experiment the author pointed out that the large dose glucocor-ticosteroids may be useful during the early stage of septic shock

Hospital information system (HIS) has already become indispensable for modern hospital. Data from it being important for patients and hospitals, their safe storages have been important missions for HIS. The means of storages introduced in detail, two kinds of methods are put forward according to the characteristics of HIS data, which apply SAN and NAS to HIS.

Objective To study the MR features and diagnostic value of tethered cord syndrome(TCS).Methods MR findings of 30 cases with TCS were reviewed.All cases were confirmed by operation and pathology.Results In 30 cases,the levels of the tips of the conus were below the middle of L 2 vertebral body.Spinal cords were fixed as result of being drawn.MR findings were as follows:Single thickened and tightened filum terminale(4 cases,13%);Lipoid buld(13 cases,43%);Myeloey stocele(3 cases,10%);Meningcele(4 cases,13%);Tumor in spinalcannal(6 cases,20%) Included neurofibroma (2 cases),dermoid cyst (2 cases) and epidermoid (1 cases),teratoma(1 cases).Conclusion MRI with high soft tissue resolution and mutiple sections imaging is superior to any other imaging means,it can clearly demonstrate the position and shape of the spinal cord conus as well as the associated malformation.

The issue of medical morality got more and more attention in the change of modern china society.It is not just an issue among the medical profession,but also bears the multi-meaning of politics,economics and culture.In the context of cruel environment of modern china society,many problems of medical morality existing among the medical profession were disclosed thoroughly.How to solve these problem concerns the health of public,and the development of china medical.In that time,many china medical professionals managed to put forward strategies to promote the development of china modern medical,which we should use for reference nowadays.

Objective To investigate the regulatory effects of resveratrol on proliferation, adhesion, migration, invasion,epithelial-mesenchymal transition(EMT), and Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling pathway of human glioma cells T98G, so as to provide theoretical basis for the related research of resveratrol in the treatment of glioma.Methods Human glioma T98G cells were cultured in vitro and divided into control group(without intervention), experimental groups(12.5, 25, and 50 μmol/L resveratrol groups), positive drug group(50 mg/L 5-fluorouracil), inhibitor group(50 μmol/L resveratrol + 50 μmol/L JAK2/STAT3 signaling inhibitor AG490) and activator group(50 μmol/L resveratrol + 0. 5 μmol/L JAK2/STAT3 signaling activator colivelin), which were intervened for 24 h. The cell viability, proliferation rate, adhesion ability, migration ability, invasion ability, EMT and JAK2/STAT3 signaling pathway related protein expression levels were analyzed by cell count(CCK-8), 5-ethynyl-2′deoxyuridine(EdU), adhesion test,scratch test, Transwell and Western blot.Results Compared with the control group, the cell viability of 12. 5 and 25 μmol/L resveratrol groups had no significant change(t = 1. 501 and 2. 167, respectively, P > 0. 05), while that of 50 μmol/L resveratrol group and the positive drug group decreased significantly(t = 7. 918 and 8. 020, respectively, P < 0. 05). The cell viability of 12. 5 and 25 μmol/L resveratrol groups increased significantly compared with the positive drug group(t = 6. 519and 5. 853, respectively, P < 0. 05), and that of 50 μmol/L resveratrol group was close to 50% and had no significant difference from that of the positive drug group(t = 0. 102, P = 0. 921, P > 0. 05). Compared with the control group, the proliferation rate, adhesion, migration, invasion ability and the protein expression of N-cadherin, vimentin, fibronectin(FN), p-JAK2,and p-STAT3 in 50 μmol/L resveratrol group and positive drug group significantly decreased(t = 4. 090-28. 510, respectively, P < 0. 05), while the expression of E-cadherin significantly increased(t = 16. 782 and 17. 291, respectively, P <0. 05). Compared with 50 μmol/L resveratrol group, the proliferation rate, adhesion, migration, and invasion ability, and the protein expression levels of N-cadherin, vimentin, FN, p-JAK2 and p-STAT3 of T98G cells in the inhibitor group significantly decreased(t = 3. 801-17. 980, respectively, P < 0. 05), while the expression level of E-cadherin protein significantly increased(t = 15. 256, P < 0. 05); the proliferation rate, adhesion, migration, and invasion ability, and the protein expression of N-cadherin, vimentin, FN, p-JAK2 and p-STAT3 of T98G cells in the activator group significantly increased(t =4. 235-27. 951, respectively, P < 0. 05), while the expression of E-cadherin significantly decreased(t = 14. 748, P < 0. 05).Conclusion Resveratrol can significantly inhibit the proliferation, adhesion, migration and invasion of human glioma T98G cells, which may be related to the inhibition of EMT process by inhibiting JAK2/STAT3 pathway signal transduction.

Epigenetics is the study of genomic structural modifications that affects gene expression without DNA sequence change.Epigenetic mechanisms for the regulation of gene expression include DNA methylation,histone modifications,and microRNAs.DNA methylation may contribute to silencing gene expression which is a major mechanism of epigenetic gene regulation.DNA methylation regulatory mechanisms in lens development and pathogenesis of cataract represent exciting areas of research that have opened new avenues for association with aging and environment.This review concludes current understanding of the major mechanisms and function of DNA methylation in lens development,pathogenesis of age-related cataract,secondary cataract and complicated cataract.By understanding the role of DNA methylation in the lens disease and development,it may help to develop a new therapeutic approach to clinical treatment of cataract.

Aged ; Angiofibroma ; metabolism ; pathology ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Inflammation ; Leiomyosarcoma ; pathology ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; Vimentin ; metabolism ; Vulvar Neoplasms ; metabolism ; pathology

Objective The androgen signaling pathway is involved in the regulation of early follicular growth and follicular atresia, and sex hormone binding globulin (SHBG) is an important factor in regulating the level of local ovarian androgen.This study was to investigate the effects of androgen on the expression of SHBG in ovarian granulosa cells.Methods Human ovarian granule cancer cells were cultured with 0 nmol/L dihydrotestosterone (DHT), 500 nmol/L DHT, or 500 nmol/L DHT + 60 μmol/L Flutamide.The expression of SHBG was detected by Western blot and immunofluorescence staining.Hyperandrogenism (HA) was induced in 6 SD rats by subcutaneous injection of dehydroepiandrosterone (DHEA) and another 6 rats were included in the vehicle control group.At 35 days after modeling, all the rats were sacrificed for measurement of the level of SHBG in the blood from the inferior caval vein by ELISA, the expression of SHBG in the ovarian granulosa cells by immunohistochemistry, and expressions of androgen receptor (AR) and SHBG in the liver by Western blot.Results At 24 hours after modeling, the expression of AR was significantly upregulated in the 300, 400, and 500 nmol/L DHT groups as compared with the 0 nmol/L DHT group (1.06±0.02, 1.61±0.11, and 2.38±0.14 vs 1.06±0.03, P<0.05), and so was that of SHBG, increasing in a concentration-dependent manner, most significantly at 500 nmol/L (P<0.01).Both the expressions of AR and SHBG proteins remarkably elevated in the 500 nmol/L DHT group in comparison with the 0 nmol/L DHT group (P<0.01), but markedly downregulated as compared with the 500 nmol/L DHT + 60 μmol/L Flutamide group (P<0.05).Immunofluorescence staining showed that DHT promoted while the addition of Flutamide inhibited the expressions of AR and SHBG.Immunohistochemical staining of the ovarian tissue revealed a high level of SHBG in the HA rats.Compared with the control group, the HA animals exhibited a significantly decreased expression of serum SHBG (4.80±0.35 vs 2.41±0.14, P<0.01) and that in the liver, but a markedly increased level of the AR protein (P<0.05).Conclusion Activation of the androgen signaling pathway by DHT can promote the expression of SHBG in rat ovarian granulosa cells.

Epidermal growth factor receptor (EGFR) is necessary in normal epithelial cell growth. Ab-errant EGFR expression is linked with increased cell proliferation and decreased apoptosis. Mutations and over-expression of EGFR are common features of many cancers. Therefore, Therapeutic agents that target the EGFR signal pathway, such as small-molecule tyrosine kinase inhibiiors and monoclonal antibodies are now advanced in clinical development. EGFR-targeted therapies of non-small-cell lung cancer (NSCLC) have been studied as a new way.