Objective To explore the dynamic changes of abdominal and peripheral arteries in the patients with familial hypercholesterolemia(FH). Methods The intima-media thickness(IMT), stenotic degree and hemodynamics change of arteries were measured by ultrasonography in three children in 3 FH pedigrees. Results The plaques number and IMT of carotid and femoral arteries in the patients with FH significantly increased. The IMT of vertebral artery, subclavein artery, abdominal aorta, renal artery, illac artery and popliteal artery did not obviously changed. Conclusion Noninvasive vascular ultrasound was a preferred imaging technique to follow up the vascular change in the patients with FH.

OBJECTIVETo study effects of autogenous femoral head bone grafting combined with total hip replacement for the treatment of old center dislocation of hip joint, and to evaluate the superiority of acetabular defect reconstruction with autogenous bone.

METHODSFrom March 2008 to December 2013, 16 patients (16 hips) with old center dislocation of hip joint were treated with autologous bone graft combined with total hip replacement. There were 11 males and 5 females, ranging in age from 41 to 72 years old, with an average of 56.3 years old. The duration of the disease ranged from 3.6 to 37.2 years. Twelve patients had injuries caused by traffic accidents and 4 patients were caused by falling down. The hip joint pain at the first month after operation, Harris score and hip joint movement of all patients were observed by using electronic case follow-up system.

RESULTSAll the patients were followed up, and the duration ranged from 11 to 78 months, with an average of 27.3 months. The postoperative hip joint movement of (56.2±23.4)° VAS 86.3±7.2 and Harris score 32.6±12.6 were all better than preoperative (181.8±17.6)°, 11.1±2.6 and 86.3±7.2. The joint pain was reduced and the function limitation was improved after operation, and the prosthesis position was good.

CONCLUSIONWhen the autologous femoral head bone grafting combined with total hip arthroplasty is used to treat old hip central dislocation, the initial and long-term stability of acetabular bone grafting is good, and the technique can avoid allogeneic bone complications, bone source rationally and reduce the economic burden of patients.

Adult ; Aged ; Arthroplasty, Replacement, Hip ; methods ; Female ; Femur Head ; transplantation ; Humans ; Joint Dislocations ; surgery ; Male ; Middle Aged ; Transplantation, Autologous

Fibrosis is a pathological process characterized by tissue scars and can occur in many organs of the human body. Organ fibrosis is manifested by increased fibrous connective tissue and reduced parenchymal cells in organ tissues, which can lead to destruction of organ structures and reduced function, which seriously endangers human health. Current strategies for treating organ fibrosis include: blocking the transforming growth factor-β1 (TGF-β1)/Smad signaling pathway, anti-inflammatory, regulating the sphingosine kinase 1/sphingosine-1-phosphate (SK1/S1P) signaling pathway, antagonizing vasoactive peptide receptors, enzyme inhibitors, kinase inhibitors, inhibitors of cellular signaling pathway, regulation of metabolic pathways, and mesenchymal stem cell therapy. In the review, the treatment strategies for organ fibrosis and the latest developments in the research of anti-organ fibrosis drugs are summarized to provide a reference for the development of anti-organ fibrosis drugs.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; epidemiology ; Young Adult

BACKGROUND:It remains controversial in term of efficacy for the lightweight mesh and heavyweight mesh in inguinal hernia repair.
OBJECTIVE:To compare the clinical therapeutic effects of lightweight mesh and heavyweight mesh in open methods of inguinal hernia repair with Meta-analysis.
METHODS:Comprehensive electronic search strategies were developed using the fol owing electronic databases:PubMed, Cochrane Library, EMBASE, Medline, Ovid, CNKI, VIP, Wanfang and FMJS. The Literature published before February 2013 was searched. The randomized control ed trials about comparing lightweight mesh and heavyweight mesh in open methods of inguinal hernia repair were included. A data-extraction sheet was developed based on the preset standards. The data from eligible studies were pooled using RevMan5.1 software through Meta-analysis.
RESULTS AND CONCLUSION:Eighteen trials with a total of 4 450 hernias met the inclusion criteria. The meta-analysis showed that there was a statistical difference between lightweight mesh group and the heavyweight mesh group on short-term pain [odd ratio (OR)=0.57, 95%confidence interval (CI) (0.43, 0.74), P<0.05] and a reduced risk of developing foreign body sensations [OR=0.49, 95%CI (0.35, 0.69), P<0.05]. No significant differences were found between the two groups in recurrence rate, testicular atrophy, seroma, hematoma, wound infection, urine retention (P>0.5). According to limited evidence, there are some findings as fol ows:the lightweight mesh is of feasibility, safety and effectiveness for inguinal hernia repair. Because of the limits of sample and quality, more large-sample and high-quality trials are required to make a definite clinical evidence to use lightweight mesh for inguinal hernia repair.

Objective To evaluate contrast-enhanced ultrasonography (CEUS) in diagnosis of small hepatocellular carcinoma (SHCC) ( ≤ 2. 0 cm) with liver cirrhosis after contrast-enhanced CT (CECT) examination. Methods Forty five patients with liver cirrhosis received CECT and CEUS examinations before operation or needle biopsy and the diagnosis was confirmed by pathological examination.CEUS and CECT findings of 51 liver space-occupying lesions from 45 patients were retrospectively analyzed.Results Among all 51 lesions detected CEUS and CECT found 49 and 35, respectively. The typical characteristics of SHCC were "fast-in and fast-out" and "fast-in and slow-out". The sensitivity of CEUS and CECT in diagnosis of SHCC was 88. 9% (32/36) and 69. 4 % (25/36) respectively ( x2 = 3. 02, P =0. 08);the diagnostic accuracy was 84. 3 % (43/51 ) and 56. 9% (29/51 ) respectively ( x2 = 1.46, P =0. 22). Among 16 lesions missed by CECT, 12 were detected by CEUS. Conclusions CEUS and CECT show the similar diagnostic rate for typical SHCC ,however, CEUS is more sensitive for atypical lesions. With high time resolution, CEUS have advantages for follow-up study of benign liver lesions.

Objective With a newly developed ultrasoundbased technique named as echo tracking,to determine the carotid stiffness and compliance in familial hypercholesterolemia(FH) patients.Methods The study population included 8 FH patients with 3 male and 4 female at a mean age of((17.22)?(5.69)) years and 7 normal controls. With the echo tracking technique,the quantitative parameters of Ep,? and the arterial compliance(AC) were detected.Results Ep,? and AC of the subtotal FH patients were higher than that of controls,the statistical significance was found in ? of both common carotid arteries,Ep of right common artery and AC of left artery in two groups.Conclusions The echo tracking technique may be a promising modality for noninvasive estimation of the atherosclerotic proceeding in FH patients.

Objective To observe the external morphology of Lepidoglyphus destructor hypopus under an optical microscope. Methods The samples were collected in a store of Chinese medicinal herbs in Huainan City in September,2016,the L. de-structor and the hypopus were isolated,and then made of slide specimens. The slide samples were prepared and observed under an optical microscope. Results The L. destructor hypopus and protonymph were found. The inactive hypopus was oval in shape, the feet were not well-developed,there was a distinct transverse seam on its back,and there were 2 pairs of genital sensory or-gans. Conclusion The optical microscopy shows the morphological characteristics of L. destructor hypopus,which can provide the basis for the biological classification and the prevention.

Objective To investigate the species of Limacodidae and the biological habits on Prunus cerasifera and sycamore in the urban area of Wuhu City,so as to provide the reference for the prevention and control of the diseases caused by Limacodi?dae. Methods Two observation places were selected in the urban area of Wuhu City,and the survey period of the first observa?tion place(A)was from 2014?5?4 to 2014?11?2,and eucleid caterpillar was observed and collected every Sunday(a total of 27 times);the survey period of the second observation place(B)was from 2014?1?5 to 2014?12?28,and eucleid caterpillar was ob?served and collected every Sunday(a total of 52 times). The eucleid caterpillars were collected by the visual inspection and arti?ficial direct collecting method,and then they were identified and classified by the naked eye direct observation and the direct ob?servation with the help of the anatomical lens in the laboratory. Results There were seven kinds of identified Limacodidae from the two observation places and their occurrence regularity,the species composition ratio,and natural enemies were understood. Conclusions Limacodidae larvae not only are the defoliator of the ornamental forests,but also harm the health of residents, that is they can cause eucleid larva dermatitis. Therefore,we should strengthen the prevention and control of Limacodidae,re?ducing its impact on the lives of the residents.

Objective:To generate rabbit polyclonal antibody against human Argonaute2 (Ago2) protein and to identify its functional characterization for determination of differential expression and cellular localization of Ago2 protein in various cell lines.Methods:DNAstar software was applied for searching the high antigenicity region of Ago2 gene sequence termed k-Ago2.Prokaryotic expressing plasmid was constructed and transformed to E.coli BL21 (DE3) to induce expression by IPTG.The fusion protein was injected into rabbits subcutaneously to produce polyclonal antibodies after purification by gel regaining.ELISA was operated to detect antibody titer.Western blot was used to identify the specificity and sensitivity of the antibodies and detect the differential expression of Ago2 protein in various cell lines.Meanwhile,immunofluorescence experiments were arranged to show cellular localization of Ago2 protein.Results:The prokaryotic expressing plasmid was constructed correctly.K-Ago2 protein was expressed and purified,and then rabbit polyclonal antibodies against Ago2 were generated after immunization with k-Ago2 protein.The titer detected by ELISA was 1∶19 000.Western blot results demonstrated the high specificity of the antibodies.Finally,we successfully observed the differential expression and cellular localization of Ago2 protein in various cell lines.Conclusion:The polyclonal antibody against Ago2 protein has been achieved successfully.It will be propitious for the intensive study of the RNAi mechanism and even profound clinical application.