Purpose: To summarize the efficiency and the experience of the puncture aspiration and sclerosing therapy of hepatic and renal cysts. Materials and methods: 27 patients were treated with percutaneous aspiration and alcohol injection of the cystic cavity using 20G Greene needles. Among the 27 patients, 11 were with hepatic cyst, 15 with renal cyst, and 1 with polycystic kidneys. The size of cysts ranged from 3.5 to 12 cm, and the amount of aspirated fluid varied from 21 to 550ml. Pure alcohol (99. 7%) was injected into the cavities after apiration. Results: All the puncture procedures were successful, and no major complications occurred, The clinical symptoms were relieved or disappeared after the treatment. 18 cases were followed for 3 months to 5 years. The efficient indices: Grade I one case (polycystic kidneys), Grade Ⅱ 4 cases and Grade Ⅲ 13 cases. Conclusion: CT guided percutaneous puncture aspiration and sclerosing treatment of hepatic and renal cysts can be considered as an effective alternative to surgical treatment. The simple aspiration of polycystic kidneys is very helpful in relieving symptoms and improving renal functions.

The degradation kinetics of chlorogenic acid (5-CQA), cryptochlorogenic acid (4-CQA), and neochlorogenic acid (3-CQA) in aqueous solution at 37 degrees C and different pH values (7.05, 7.96, 9.25) were investigated in the present work. The results indicated that 3-, 4- and 5-CQA tended to remain stable in acidic pH circumstance, and unstable in neutral and alkaline pH circumstance. With the increase of the alkalinity, the degradation of 3-, 4- and 5-CQA was increased leading to a less amount of total CQA and was satisfactorily described by the Weibull equation. Meanwhile, caffeic acid was not detected after the degradation of CQA. Moreover, the degradation of 3-CQA and 5-CQA tended to be converted to 4-CQA, and the degradation of 4-CQA tended to be converted to 3-CQA rather than 5-CQA. The comparison of the degradation kinetics parameters of 3-, 4- and 5-CQA at neutral and alkaline pH values showed that the orders of the rate constant (k) values were 4-CQA > 3-CQA > 5-CQA, while the orders of the degradation half life (t½) values were 4-CQA < 3-CQA < 5-CQA, indicating the orders of the stabilities of 3-, 4- and 5-CQA at 37 degrees C and neutral and alkaline pH values were 4-CQA < 3-CQA < 5-CQA.
Caffeic Acids ; Chlorogenic Acid ; analogs & derivatives ; chemistry ; Hydrogen-Ion Concentration ; Kinetics

The degradation kinetics of chlorogenic acid (5-CQA), cryptochlorogenic acid (4-CQA), and neochlorogenic acid (3-CQA) in aqueous solution at 37 degrees C and different pH values (7.05, 7.96, 9.25) were investigated in the present work. The results indicated that 3-, 4- and 5-CQA tended to remain stable in acidic pH circumstance, and unstable in neutral and alkaline pH circumstance. With the increase of the alkalinity, the degradation of 3-, 4- and 5-CQA was increased leading to a less amount of total CQA and was satisfactorily described by the Weibull equation. Meanwhile, caffeic acid was not detected after the degradation of CQA. Moreover, the degradation of 3-CQA and 5-CQA tended to be converted to 4-CQA, and the degradation of 4-CQA tended to be converted to 3-CQA rather than 5-CQA. The comparison of the degradation kinetics parameters of 3-, 4- and 5-CQA at neutral and alkaline pH values showed that the orders of the rate constant (k) values were 4-CQA > 3-CQA > 5-CQA, while the orders of the degradation half life (t½) values were 4-CQA < 3-CQA < 5-CQA, indicating the orders of the stabilities of 3-, 4- and 5-CQA at 37 degrees C and neutral and alkaline pH values were 4-CQA < 3-CQA < 5-CQA.

Purpose To explore the value of postoperative stimulated thyroglobulin (ps-Tg) level in predicting functional metastasis in patients with differentiated thyroid carcinoma (DTC) before 131I therapy,in order to guide 131I therapy.Materials and Methods 101 DTC patients who accepted total thyroidectomy and lymphadenectomy in the First People's Hospital of Lianyungang were retrospectively analyzed.The ps-Tg level after DTC surgery was detected 1 day before 131I therapy,and 131I-SPECT/CT scan was performed 5-7 days after 131I therapy.The presence of functional metastasis was confirmed on the basis of 131I-SPECT/CT imaging,and the patients were assigned into non-metastic (M0) group and metastatic (M1) group.The ROC curve was used to evaluate the value of ps-Tg in predicting functional metastasis.Results The ps-Tg level in M 1 group was higher than that in M0 group,and the difference was statistically significant (U=328.00,P＜0.001).The area of ps-Tg value under the ROC curve was 0.870,and the diagnostic cut-off point was 40.60 ng/ml.The sensitivity,specificity and accuracy was 72.34％,100.00％ and 87.13％,respectively.Concltsion The ps-Tg value can be used as an effective indicator to predict functional metastasis,and is able to guide 131I therapy.

Objective To explore the optimal semi-quantitative uptake ratio for differentiation between benign and malignant lung lesions with 18F-FDG coincidence imaging.Methods One hundred and thirty-seven patients (89 males,48 females,age range:33-78 years) with lung diseases underwent 18 FFDG coincidence imaging.The maximum radioactivity counts of the lung lesions (T),normal chest wall soft tissues (NT1) and the contralateral lung tissue (NT2) were measured.The ratios of R1 (T/NT1) and R2(T/NT2) were calculated.The optimal threshold values of R1(cutoff)and R2(cutoff) were identified by ROC curve analysis.Using the optimal threshold,the sensitivity,specificity and accuracy were calculated.Results The optimal threshold values R1(cutoff)and R2(cutoff) were identified as 3.58 and 4.40.The sensitivity,specificity and accuracy were 90.0％(90/100),89.2％(33/37),89.8％(123/137) according to R1(cutoff) and 90.0％ (90/100),78.4％(29/37),86.9％(119/137) according to R2(cutoff) Conclusion Based on which the chest wall soft tissue is taken as a reference point,the optimal threshold value of T/NT1 is 3.58 in differentiation between benign and malignant lung lesions with 18F-FDG coincidence imaging.

Objective To explore the use of marginal kidneys from DBD (donation after brain death) donors and the indication for adult dual kidney transplantation.Methods Two pairs of graft kidneys were procured from two marginal adult donors.Dual kidney transplants were performed in two recipients.In each recipient,two kidneys were implanted in unilateral site of right lower quadrant and placed extraperitoneally,two separate extravesical ureterneocysto-anastomoses were performed.Results Delayed graft function (DGF) combined with acute rejection occurred in two cases,and all two cases recovered after treatments.Lymphocele and hematoma occurred in one case.No graft embolism and no urinary leak happened.Conclusions Adult dual kidney transplant offers an important use of kidneys from marginal donors to increase the number of organs available for transplantation.

Objective To compare the immediate effects of extracorporeal shock wave therapy with that of joint mobilization plus short wave therapy in treating scapulohumeral periarthritis, and to elucidate the mechanism of extracorporeal shock wave therapy and how it should be used. Methods Forty-seven patients diagnosed with scapulohumeral periarthritis were selected and randomized into two groups, a treatment group (n = 26) treated with extracorporeal shock wave therapy, and a control group ( n = 21 ) treated with joint mobilization plus short wave therapy. The short form of the McGill pain questionnaire (SF-MPQ) and range of motion (ROM) measurements were used to evaluate shoulder function. Results Compared with baseline, both groups improved significantly af-ter treatment, but the immediate effect in the treatment group was superior to that in the control group. Concln-sions As a safe, time-saving and effective therapy, extracorpnreal shock waves could be used to treat scapulo-humeral periarthritis.

BACKGROUND: There are some adverse reactions when bone morphogenetic protein-2 is applied independently in clinic; therefore, it is urgent to develop an ideal carrier material to reduce the incidence of adverse reactions induced by bone morphogenetic protein-2. OBJECTIVE:To comprehensively understand the performance of different kinds of bone morphogenetic protein-2 carrier materials, and to summarize their research progress in the field of spinal fusion. METHODS:The literatures about bone morphogenetic protein-2 scaffolds in spinal fusion were retrieved by the first author from PubMed and Wanfang datebase durings July 1, 1995 to July 1, 2015 with the key words of“tissue engineering, bone morphogenetic protein-2, spinal fusion, scaffold” in English and Chinese, respectively. Repetitive studies were excluded. RESULTS AND CONCLUSION:Bone morphogenetic protein-2 carrier materials mainly include four categories: natural biological materials, synthetic organic materials, synthetic inorganic materials and composite materials, each of which has several representative scaffold materials. Each category has their own shortcomings when applied in animal spinal fusion, for instance, the mechanical properties of natural biological materials are generaly poor; synthetic organic materials may cause inflammatory reactions; the mechanical strength of synthetic inorganic materials is poor and the production process of composite materials is relatively complicated. Therefore, the selection of bone morphogenetic protein-2 carrier materials stil needs further experimental study.

Objective To investigate the effects of the sense and antisense testes-signal transduction and activator of RNA (T-STAR) gene on the colon cancer cell line HCT-116. Methods The sense and antisense T-STAR gene was stably transfected into HCT-116 cells with lipofectamine. The expression level of T-STAR in those cells was detected by Western blotting and the growth velocity and proliferation of those cells by cytokinetics. Results The growth velocity and proliferation decreased after transfection of the sense T-STAR gene, but increased after transfection of the antisense T-STAR gene. Conclusion T-STAR gene can inhibit the growth velocity and proliferation of HCT-116 cells.

Objective To investigate the changes of telomerase activity after knocking down endogenous expression of T-STAR (testes-signal transduction and activator of RNA) gene in human lung adenocarcinoma cell line A549 by antisense strategy. Methods The mRNA and protein expression of T-STAR gene were determined by RT-PCR and Western blotting, and the telomerase activity was measured by PCR-ELISA, after transfection of T-STAR antisense gene into A549 cells with lipofectamine. Sense pcDNA-STAR and blank pcDNA3.1 transfection served as control. Results The expression of T-STAR gene was significantly inhibited at mRNA and protein level, and the telomerase activity was significantly decreased. Conclusion The down-regulation of telomerase activity may result from inhibition of T-STAR gene expression in lung adenocarcinoma A549 cells.