BACKGROUND: Malnutrition and overnutrition are the two problems during growth and development of high-school students.OBJECTIVE: To understand the situation and relevant factors of malnutrition and overnutrition of high-school students.DESIGN: Transverse investigation was designed.SETTING: Health Supervision Institute of Chongqing Health Bureau; Department of Nutrition & Food Hygiene, Chongqing University of Medical Sciences; the Eighth Middle School of ChongqingPARTICIPANTS: Totally 896 students in 15 classes were collected from one full-time middle school in Shapingba district in Chongqing by cluster sampling method from March to May 2004. The students were excluded if they suffered from hereditary metabolic disease and secondary pathological malnutrition or obesity due to trauma or other diseases. All of participants were in the known in the investigation. Of those, 826 students filled up the questionnaire in detail (the effective rate was 92.19%), in which, 414 students were male and 412 students female, aged varied from 13 to 18 years.METHODS: The students chose were in the investigation with questionnaire and the contents of it included basic physical situation, state of sickness, situation of parents, dietary behavior, nutritional knowledge, dietary habits, etc. The students filled up the questionnaire by them own and handed over on the spot. The evaluation of student nutrition state adopted the heights and body masses in recent physical examination scale, in reference to the criteria in "height and body mass of Chinese students aged from 7 to 22 years". The standard body mass was 80% of body masses of people with same height, based on which, the standard body mass was 100%, that less than the standard by 90% was taken as malnutrition and more than it by 120% was taken as obesity. Non-conditional logistic stepwise regression was used to analyze the data so as to probe into the factors of malnutrition and obesity in middle-school students.MAIN OUTCOME MEASURES: [1] Morbidity of malnutrition and obesity in middle-school students. [2] Factors of malnutrition and obesity in middle-school students.RESULTS: Totally 826 students entered result analysis. [1] Morbidity of malnutrition and obesity: it was 9.44% and 24.21% respectively, in which,the morbidity of malnutrition.for female was higher than male [4.84%,14.08% (x2=15.1036, P ＜ 0.05)] and the morbidity of obesity in male was higher than female [30.09%, 18.2% (x2=10.5737, P ＜ 0.05)]. [2] Factors of malnutrition and obesity: significant correlation was present between malnutrition and 5 factors, named sex, indulgence in or being choosy food,sleeping time, breakfast and animal internal organs (P ＜ 0.05-0.01). Significant correlation was present between obesity and 5 factors, named sex,body mass index of father, eating velocity, midnight snack and indulgence in or being choosy food (P ＜ 0.05-0.01).CONCLUSION: It is discovered in the investigation that the morbidity of malnutrition and obesity in middle-school students are very high and that is all closely related to sex and indulgence in or being choosy food. In order to correct malnutrition and obesity in middle-school students, it is necessary to provide correct nutritional knowledge and help them arranging diet rationally and establishing healthy life pattern.

Objective To investigate nursing students and nurses' knowledge, attitude and behaviors of standardized treatment of medical waste, so as to develop more constructive advices in future. Methods The survey was conducted in 80 nursing students and 85 nurses in one of the Beijing' s third level 1 class hospitals.The data was analyzed with SPSS 17.0 software. Results Nursing students' scores of knowledge, attitude and behavior were significantly lower than nurses' ( t =- 6.986, - 5.336, - 3.862 ; P ＜ 0. 05 ) ; Nursing students with higher education levels showed significantly higher scores of knowledge ( F =9. 992, P ＜ 0. 05 ), but there' s no significant difference with scores of attitude or behavior( F =1. 279,1. 595 ; P ＞ 0. 05 ) ; Nurses with different education levels, length of service and professional titles showed no significant difference with scores of knowledge, attitude and behavior(P ＞ 0. 05). Scores of nursing students and nurses in behavior were low, and needs to be raised. Conclusions Adequate knowledge is foundation and good attitude is propulsion, they two will be helpful to increase the quality of treatment of medical waste with systematic management and training of the hospital.

Hepatitis, Viral, Human ; drug therapy ; Humans ; Prostaglandins E ; therapeutic use ; Randomized Controlled Trials as Topic ; Research Design

Alternative Splicing ; Apoptosis ; Cell Cycle ; Cell Proliferation ; Cyclin-Dependent Kinase Inhibitor p21 ; metabolism ; Hep G2 Cells ; Humans ; Inhibitor of Growth Protein 1 ; Intracellular Signaling Peptides and Proteins ; genetics ; metabolism ; physiology ; Nuclear Proteins ; genetics ; metabolism ; physiology ; Plasmids ; Protein Isoforms ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Recombinant Proteins ; genetics ; metabolism ; Transfection ; Tumor Suppressor Protein p53 ; metabolism ; Tumor Suppressor Proteins ; genetics ; metabolism ; physiology ; bcl-2-Associated X Protein ; metabolism

Objective To evaluate the outcome of combination of intensive preconditioning regimen allo-HSCT with imatinib for treatment of Ph chromosome positive acute lymphocyte leukemia (ALL). Methods Between 2009 and 2010, 8 patients diagnosed as Ph+ ALL received allo-HSCT from HLA identical sibling during complete remission. Imatinib was added into the therapies of 5 patients.Seven patients received the intensive preconditioning regimen based on BuCy2, one patient received the regimen of TBI-Cy. A median of 6. 02 × 108/kg mononuclear cells and 3. 14 × 106/kg CD34+ cells were transfused. GVHD prophylaxis included cyclosporine A and methotrexate. Results All patients were well tolerant to the regimen without serious regimen-related toxicity. The median time of ANC≥0. 5 × 109/L was 15. 5 days, and that of PLT≥20 × 109/L was 19 days. Thirty days after allo-HSCT, all patients got donor engraftment successfully. Among 8 cases, 4 cases presented acute GVHD, 2 developed degree Ⅰ , one developed degree Ⅱ , and one developed degree Ⅳ. Seven patients were alive 100 days after allo-HSCT, 3 of whom presented chronic GVHD. At the end of following-up period, 6 patients were alive, among them, 3 patients were alive without relapse; 3 patients relapsed; Two patients died, one from acute GVHD, and one from leukemia relapse. Conclusion Combined intensive preconditioning regimen allo-HSCT with Imatinib was an effective treatment for Ph+ ALL, but the effect of anti-chronic GVHD of imatinib should arouse certain attention.

Objective To study exogenous expression and subcellular localization of wild type (WT ) and mu-tant PAX3 proteins in vitro by generating their expression plasmids for further study of pathogenesis of Waarden-burg syndrome (WS) .Methods The plasmids pECE-PAX3 and pcDNA3 .0-HA were ligased after they were cut by double enzyme digestion using molecular cloning technique to generate recombinant eukaryotic expression plasmid pcDNA3 .0-PAX3-HA ,which was as a template to generate expression plasmids pcDNA 3 .0 -H80D -HA and pcDNA3 .0-H186fs-HA of novel mutations H80D and H186fs of PAX3 gene .All constructs were verified by di-rect nucleotide sequencing .NIH3T3 cells were transfected transiently with the expression plasmids of PAX3 ,H80D and H186fs respectively .The exogenous expression of WT PAX3 protein and mutant H80D ,H186fs proteins were analysed using Western blot assay ,while their subcellular distribution were observed using immunofluorescence as-say .Results The DNA sequences of expression plasmids of PAX3 and its mutant H80D ,H186fs were correct . Both WT and mutant PAX3 proteins were detected at the expected size .WT PAX3 and H80D proteins were only lo-calized in the nucleus ,whereas H186fs protein showed aberrant localization in both cytoplasm and nucleus .Conclu-sion We successfully generated the recombinant eukaryotic expression plasmids of PAX 3 gene and its mutants and drew preliminary conlusion of gene mutation having effect on subcellular distribution of WT PAX 3 proteins in vitro , which lays experimental basis for further study of the moceluar mechanism of WS caused by PAX3 gene mutations in China .

A research combined trickling filter system and active sludge aeration system was applied in the treatment of industrial wastewater from gas-generating with heavy oil. The wastewater contained both high contents of NH+4-N and mixed hydrocarbons including various PAHs. Its BOD5/COD ratio was less than 0.3 and belongs to recalcitrant, toxic wastewater. The results showed a touch-growth biofilms system was formed on the porous packing material and it played a key role in the decrease of toxicity of the influent. It could also improve the biodegradability of the wastewater.

The article entitled "Differential expression of exosomal miRNAs in osteoblasts in osteoarthritis" published on Journal of Central South University (Medical Science), in Volume 43, Issue 12, 2018 (DOI: 10.11817/j.issn.1672-7347.2018.12.003) may have an unclear risk of bias due to insufficient understanding for some results. Further experimental studies are needed. We all agree to retract this article, and apologize to the Journal and readers for the possible negative impact.

ObjectiveTo investigate the protective effect of hepatocyte growth-promoting factor (PHGF) against liver ischemia-reperfusion injury in rats and its mechanism of its action. MethodsA total of 80 healthy male Sprague-Dawley rats were randomly divided into experimental group (PHGF group) and control group (NS group), with 40 rats in each group. A rat model of liver ischemia-reperfusion injury was established by 70% liver ischemia caused by the occlusion of blood flow in the middle and left lobes of the liver, with an ischemia time of 21 minutes. The rats in the PHGF group were given intraperitoneal injection of PHGF for intervention before surgery, and those in the NS group were given an equal volume of normal saline. Serum and liver tissue samples were collected before surgery and on days 1, 3, 5, and 7 after surgery, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBil) were measured; HE staining was used to observe pathological changes; real-time PCR was used to measure the mRNA expression of mitochondrial transcription factor A (TFAM) in the liver. The independent samples t-test was used for comparison of continuous data between two groups. ResultsHE staining showed that compared with the NS group, the PHGF group had significantly lower degrees of hepatocyte swelling, inflammatory cell infiltration, and hepatocyte necrosis under a light microscope. Liver biochemistry showed that on days 1, 3, 5, and 7 after surgery, the PHGF group had significantly lower serum levels of ALT, AST, and TBil than the NS group (t=11.879, 16.019, 22168, 10.235, 9.041, 12.936, 18.759, 8.142, 10.108, 11.014, 13.245, and 9.968, all P＜0.001). Real-time PCR showed that on days 1, 3, and 5 after surgery, the PHGF group had a significantly higher mRNA level of TFAM in the liver than the NS group (t=7998, 14.764, and 13.861, all P＜0.001). ConclusionPHGF preconditioning exerts a protective effect against liver ischemia-reperfusion injury in rats, possibly by upregulating the expression of TFAM to alleviate liver ischemia-reperfusion injury.

Objective To investigate the status of social support, anxiety and depression of the Amyotrophic lateral sclerosis ( ALS) and its related influencing factors, to provide basis for the treatment and nursing. Methods To investigate 56 amyotrophic lateral sclerosis patients with the Social Support Rating Scale (SSRS), Self-evaluating Measurements of HAMD and HAM A, to statistically analyze social support, anxiety and depression of 56 patients with SPSS 16.0 Statistical Package. Results The total score of social support of the Amyotrophic lateral sclerosis patients was 24 to 53. The average score was (39. 36 ±6. 15) , which was higher than the value of domestic norm (P <0. 05). The incidence rates of anxiety and depression among ALS patients were 26. 79% and 33. 93%. Conclusions The overall status of social support for the Amyotrophic lateral sclerosis patients is good, patients who get more social support get less anxiety and depression. More social support is need for the amyotrophic lateral sclerosis patients who are women, elderly, farmer, ordinary worker and get lower educational level,