BACKGROUND:Previous studies demonstrated that ischemia can be compensated by establishing collateral circulation and microangiogenesis,and exercise training can ameliorate blood supply of ischemic lower limb.However,whether exercise training accelerate establishment of collateral circulation remains poorly understood.OBJECTIVE:To explore the mechanism of exercise training on accelerating microangiogenesis of rat ischemic lower limb.METHODS:Sprague-Dawley rats were randomly divided into the exercise training,model and sham-surgery groups.All animals were established left lower limb ischemia models except those in the sham-surgery group.Rats in the exercise training group were forced running 30 minutes per day at 1 week after model preparation and those in other groups were performed daily activities.The adductor of ischemic lower limb was obtained for the examination of microvessel density and the expressions of vascular endothelial growth factor and basic fibroblast growth factor.Simultaneously,bone marrow-derived endothelial progenitor cells were harvested for detection of microangiogenesis.RESULTS AND CONCLUSION:Number of endothelial progenitor calls,expressions of vascular endothelial growth factor and basic fibroblast growth factor,as well as microvessel density of the exercise training group was obviously greater than those in the model and sham-surgery groups(P<0.01).Compared with the model and sham-surgery groups,in vitro vasculogenesisof bone marrow-derived endothelial progenitor cells in the exercise training group was increased(P<0.05).These findings demonstrated that lower limb ischemia increases microangiogenesis,and exercise training enhances this effect.

Objective: To observe the effect of acupuncture on serum peptide YY (PYY) and nesfatin-1 in obese patients with insulin resistance. Methods: Ninety-eight obese patients with insulin resistance were divided into a control group and an observation group by the random number table method, with 49 cases in each group. The control group received exercise and dietary interventions, and the observation group received additional acupuncture treatment to the exercise and dietary interventions. The body mass index (BMI), body fat percentage, fasting insulin (FINS), fasting plasma glucose (FPG), homeostasis model assessment for insulin resistance (HOMA-IR), triglyceride (TG), total cholesterol (TC), and serum PYY and nesfatin-1 levels were compared before and after treatment. The efficacy was evaluated after treatment. Results: The total effective rate was significantly higher in the observation group than in the control group (P<0.05). After treatment, the BMI, body fat percentage, and serum TG and TC levels decreased significantly in both groups (P<0.05), and were significantly lower in the observation group than those in the control group (P<0.05). The FINS, FPG, HOMA-IR, and serum PYY and nesfatin-1 levels of the control group were not significantly changed after treatment (P>0.05). The FINS, FPG and HOMA-IR of the observation group decreased significantly after treatment (P<0.05), and were lower than those in the control group (P<0.05). The serum PYY and nesfatin-1 levels of the observation group increased significantly after treatment (P<0.05), and were higher than those in the control group (P<0.05). Conclusion: Based on exercise and dietary interventions, acupuncture is effective for obese patients with insulin resistance. It can reduce the BMI, body fat percentage, blood lipids, blood glucose, and serum insulin levels and improve insulin resistance. The action may be associated with the up-regulation of serum PYY and nesfatin-1.

Geographic distribution of Polyporus umbellatus was predicted by using distribution records. Based on 42 distribution records from 12 provinces and bioclimatic data (1950-2000), georaphic distribution of P. umbellatus was modeled using Maxent. The results showed thatthe Receiver Operating Characteristic (ROC) curve analysis method was used to assess the accuracy of MAXENT model and the area under ROC curve (AUC) value of MAXENT was 0. 960 which suggested that the result of assessment was dependable. The geographic distribution pattern of were divided into three distribution block based on distribution values of 0.5-0.8: small area of Heilongjiang, Jilin, Liaoning and Hebei province, the board area of Yunnan, Guizhou and Sichuan, the southeast area of Tibet and the most area of Shanxi and Shannxi, the southeast board area of Shannxi, Gansu and Ningxia. Jackknife Test showed that average precipitation in warm seasons had the greatest contribution to the distribution gain of P. umbellatus, followed by mean temperature of driest quarter and annual mean temperature. The object suggests the potential distribution areasof P. umbellatus which is useful for the habitat conservation and introduction of P. umbellatus.
China ; Ecosystem ; Entropy ; Polyporus ; growth & development

MicroRNAs (miRNA) plays an important role in biological development and disease occurrence and development, and acts as a "main switch" in biology. Among patients of essential hypertension, around 1/3 would suffer left ventricular hypertrophy (LVH). Hence, essential hypertension becomes an independent risk factor for cardiovascular diseases. And miRNAs plays an important role in the occurrence and development of LVH. This paper reviewed the role of miRNA in regulating the stress signaling pathway, defined its impact on the occurrence of LVH, and further emphasized the opportunities and challenges of miRNA as a biomarker and therapeutic target.
Essential Hypertension ; Humans ; Hypertension ; complications ; genetics ; Hypertrophy, Left Ventricular ; complications ; genetics ; MicroRNAs ; genetics ; Risk Factors ; Signal Transduction ; genetics

Objective To study the clinical application of the complete audiologic evaluation battery consisting of ABR,ASSR and behavioral audiometry in sound field for the identification of sensorineural losses in children.Methods In the study 48 children were divided as group PTA of the children assessed by pure tone audiometry and ABR and ASSR test,and group BA of the children unable to perform on behavioral evaluations.Results The analysis of the audiometric results of group PTA showed significant correlation between the ASSR thresholds and pure tone thresholds(P＜0.01) at various frequencies.The correlation coefficients were 0.75,0.76,0.76,and 0.83 at different frequencies.The ASSR-PTA linear regression was utilized.The comparison of the ABR and ASSR results of group BA indicated that 23 ears responded in ASSR but without responses in ABR.Those without ASSR responses also had no ABR There were 116 tests in total four frequencies for the 29 ears.83 responses were obtained from ASSR,89 responses from behavior audiometry and 96 from the use of the both tests.Conclusion The comprehensive audiologic evaluation battery could be used to test younger children with severe heating loss children as compared to the pure tone audiometry alone.More detailed data can be obtained thus from children to assist in rehabilitation in a more effective mariner.

BACKGROUND: Malnutrition and overnutrition are the two problems during growth and development of high-school students.OBJECTIVE: To understand the situation and relevant factors of malnutrition and overnutrition of high-school students.DESIGN: Transverse investigation was designed.SETTING: Health Supervision Institute of Chongqing Health Bureau; Department of Nutrition & Food Hygiene, Chongqing University of Medical Sciences; the Eighth Middle School of ChongqingPARTICIPANTS: Totally 896 students in 15 classes were collected from one full-time middle school in Shapingba district in Chongqing by cluster sampling method from March to May 2004. The students were excluded if they suffered from hereditary metabolic disease and secondary pathological malnutrition or obesity due to trauma or other diseases. All of participants were in the known in the investigation. Of those, 826 students filled up the questionnaire in detail (the effective rate was 92.19%), in which, 414 students were male and 412 students female, aged varied from 13 to 18 years.METHODS: The students chose were in the investigation with questionnaire and the contents of it included basic physical situation, state of sickness, situation of parents, dietary behavior, nutritional knowledge, dietary habits, etc. The students filled up the questionnaire by them own and handed over on the spot. The evaluation of student nutrition state adopted the heights and body masses in recent physical examination scale, in reference to the criteria in "height and body mass of Chinese students aged from 7 to 22 years". The standard body mass was 80% of body masses of people with same height, based on which, the standard body mass was 100%, that less than the standard by 90% was taken as malnutrition and more than it by 120% was taken as obesity. Non-conditional logistic stepwise regression was used to analyze the data so as to probe into the factors of malnutrition and obesity in middle-school students.MAIN OUTCOME MEASURES: [1] Morbidity of malnutrition and obesity in middle-school students. [2] Factors of malnutrition and obesity in middle-school students.RESULTS: Totally 826 students entered result analysis. [1] Morbidity of malnutrition and obesity: it was 9.44% and 24.21% respectively, in which,the morbidity of malnutrition.for female was higher than male [4.84%,14.08% (x2=15.1036, P ＜ 0.05)] and the morbidity of obesity in male was higher than female [30.09%, 18.2% (x2=10.5737, P ＜ 0.05)]. [2] Factors of malnutrition and obesity: significant correlation was present between malnutrition and 5 factors, named sex, indulgence in or being choosy food,sleeping time, breakfast and animal internal organs (P ＜ 0.05-0.01). Significant correlation was present between obesity and 5 factors, named sex,body mass index of father, eating velocity, midnight snack and indulgence in or being choosy food (P ＜ 0.05-0.01).CONCLUSION: It is discovered in the investigation that the morbidity of malnutrition and obesity in middle-school students are very high and that is all closely related to sex and indulgence in or being choosy food. In order to correct malnutrition and obesity in middle-school students, it is necessary to provide correct nutritional knowledge and help them arranging diet rationally and establishing healthy life pattern.

Objective:To investigate the effects of nitric oxide(NO) and inducible nitric oxide synthase (iNOS) in the experimental abdominal aortic aneurysm (AAA) rat model.Methods:An intra-aortic elastase infusion model was used.Control rats received intra-aortic saline infusion.In the remaining groups,intra-aortic elastase infusion was used to induce aneurysm formation.These rats were treated with intraperitoneal injections of saline postoperatively(experimental group),aminoguanidine postoperatively(medicine group).Serum NO and aortic diameter were measured,Changes of histology,iNOS and MMP-9 were observed in the aortic wall.Results:Experimental group produced AAAs with significant production of iNOS,MMPs and serum NO compared with controls.In medicine group reduced aneurysm size and displayed suppression of MMPs expression,inflammatory infiltrates and serum NO production were detected.Conclusion:Expression of iNOS and MMP-9 are induced and serum NO levels are increased in experimental AAA,iNOS and NO production by iNOS play an important role with detrimental effects during experimental aneurysm development.

Objective To evaluate the changes of immunologic function in children with thalassanemia major after partial splenic embolization(PSE).Methods Immunoglobulins,T cell subsets were detected by immunologic turbidimetry and APAAP with monoclonal antibody respectively in 40 children with thalassanemia major and also in 20 healthy persons before and after PSE.These immunologic indexes were compared before and after PSE.Results The levels of IgG in serum were significantly lower one week after PSE than that before PSE.It turned to normal three weeks after PSE.The IgM,IgA levels remained unchanged during PSE.The levels of CD_3、CD_4、CD_4/CD_8 ratio in children with thalassanemia major were decreased(P

Objective To study the clinical characteristics,diagnosis and treatment of 8 children with primary ileum cecum malignant lymphoma.Methods Retrospective analysis on the clinical and pathology materials of 8 children with primary ileum cecum malignant lymphoma was performed.Results Primary ileum cecum malignant lymphom′s clinical experience had no characteristic,and radical operation was main treatment method.Chemothereapy got well to some extents.Pathology and immunity tissue chemical were B cell malignant lymphoma.Conclusions Child primary ileum cecum malignant lymphoma lack specific manifestations,and histopathology is main diagnosis methods.They have good response to binding treatment of early operation and chemothereapy.

Objective To explore the pathological basis and clinical characteristics of children with congenital choledochal cyst(CCC) combined with liver damage.Methods According to the age,38 patients with CCC were divided into 2 groups:group A had 24 cases(ranged 6 months to 3 years old);group B had 14 cases(ranged 4 to 14 years old).A comparative analysis of them was conducted retrospectively in the clinical characteristics,hepatic pathological changes,perioperatively liver function and operation.Results Group A was obvious higher than group B in jaundice,white bole stool and abdominal mass,but group B was more in stomachache.Group A was obvious higher than group B in liver function lesion,alkaline phosphatase,bilirubin increase and blood coagulation disfunction(Pa