With the expanding scale of animal cell culture and increasing demand of biopharmaceutical,the development of serum-free medium based on cell lines and products has become a major task in the field of cell engineer.The statistical methods and tools are popular in the processes of design medium and can be used to evaluate the multiple factors and their interaction scientifically and effectively.The novel microarray and proteomic analysis can improve the performance of identifying the role of medium.The aim is to provide some idea for serum-free medium study through systematically summarizing the newly and commonly used serum-free medium study and design approaches and their characteristics.

ObjectiveTo investigate the analgesic effect of TL-Ⅰ prescription.MethodsHealth ICR mouses were randomly divided into 6 groups: negative control group, positive control group, Tongtian group, large doses group,middle doses group and small doses group. The pain threshold of mouses were detected with hot plate and acetic acid writhing.ResultsTL-Ⅰ prescription can raise the threshold of pain induced by hot plate and reduce the numbers of writhing induced by acetic acid in mice, which was more significant in large doses group. ConclusionTL-Ⅰ prescription can be an effective analgesic.

ObjectiveTo investigate the sedative effect of TL-Ⅰ prescription.Methods72 healthy ICR mice were randomly divided into 6 groups: normal group, model group, positive control group, large doses Chinese materia medica group, middle doses Chinese materia medica group and small doses Chinese materia medica group. The active behavior of migraine-model-mouse was detected with tail suspension test and the analytical system of animal behavior-spontaneous movement.ResultsTL-Ⅰ prescription could prolong the quiescent time of migraine-model-mouse and shorten the active journey and time in the autonomic active box of migraine-model-mouse.ConclusionTL-Ⅰ prescription has sedative effect.

OBJECTIVETo prepare zirconia toughened nano-composite alumina ceramic powder for dental application. Physical and chemical property of the prepared material were tested, and the effect of development technology on composite powder was also studied in this study.

METHODSNano-composite alumina powder was prepared by surface-induced precipitation method. The effect of pH value and dispersing agent content on volume of alumina suspension sediment was recorded. The effect of ultrasonic time on agglomeration was measured also. X ray diffraction (XRD) was used to analyze powder phase before and after the stabilizer was added. Scanning electronic microscope (SEM) was applied for characterizing the specimen.

RESULTSThe dispersion was better at pH=9 and wt (dispersing agent) = 0.2% approximately 0.3%. Selecting proper ultrasonic time can decrease the agglomeration of powders and lower the average particle size. XRD analysis indicated that the phase composition of the prepared nano-composite ceramic powder was shown as alpha-Al2O3, t-ZrO2 and a small amount of m-ZrO2 after the addition of stabilizer. Through SEM observation, nanometer-sized ZrO2 particles (80 approximately 100 nm) were uniformly located on the surface of submicrometer alumina grains.

CONCLUSIONSBy choosing appropriate preparation method, weakly agglomerated powders with fine particle size can be obtained. The zirconia part of nano-composite powder was transmitted to partially stabled zirconia after the use of stabilizer.

Aluminum Oxide ; chemistry ; Dental Porcelain ; chemistry ; Hydrogen-Ion Concentration ; Powders ; Zirconium ; chemistry

OBJECTIVETo investigate the effects of the static magnetic field (SMF) generated by dental magnetic attachments on osteoblastic proliferations, cell cycle distribution, and apoptosis ratio.

METHODSBy simulating those of the closed-field, the closure process and the open-field Magnedisc 800 magnetic attachments respectively, the in vitro cultured rat osteoblasts were exposed continuously to 12.5, 125, 250 mT SMF. The effects of the SMF on the proliferation of the cells were examined. MTT colorimetry test was performed to detect the effect of the SMF on the vitalities of cells. Flow cytometry was utilized to analyze the cell cycles and cell apoptosis rates.

RESULTSThe SMF exposure didn't change the vital osteoblasts number, the cell cycle distribution and proliferation activities of osteoblasts. The cell apoptosis situation were not observed statistical differences.

CONCLUSIONNo matter the closed-field, the closure process and the open-field magnetic attachments respectively, continuous simulating SMF-stimulation of magnetic attachments couldn't change osteoblasts proliferation activity, cell cycle distribution, and apoptosis ratio.

Animals ; Apoptosis ; Cell Cycle ; Cell Proliferation ; Electromagnetic Fields ; Magnetic Fields ; Magnetic Phenomena ; Magnetics ; Osteoblasts ; Rats

Lack of proper study models has brought difficulties in the study of the mechanism of viral infection, life cycle and pathogenic mechanism of hepatitis C virus (HCV) and also become the major obstacles in development of efficient vaccine and new drugs for hepatitis C. In recent years, the establishment of robust HCV cell culture infection system and HCV transgenic animal provide powerful tools for the analysis of host virus interactions, which facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.
Animals ; Animals, Genetically Modified ; virology ; Disease Models, Animal ; Genotype ; Hepacivirus ; genetics ; Hepatitis C ; virology ; Mice ; Mice, Transgenic ; virology

Objective To investigate effects of the stoic magnetic field(SMF)generated by dental magnetic attachment on osteoblastic morphology and surface ultrastructure. Methods The in vitro cultured rat osteoblasts were exposed continuously to 12.5 mT,125 mT,and 250 mT static magnetic fields for 1,3,5,and 7 days. After exposed in SMF,osteoblasts were observed under a pliase contrast microscope,and then HE stained and observed under a light microscope. In addition, the cells were observed under a scanning electron microscope(SEM). ResulIs By continuous exposure,the different intensities of SMF exposure did not change the vital osteoblast growth pattern or distribution. The SEM photos showed that there were certain changes in cellular microstructures for osteoblasts after exposed to 12.5 mT for 5 to 7 days,as well as 125 mT and 250 mT for 3 to 7 days. The more exposure time increased,the more microvesicles on the surfaces of cells were observed. Conclusions Continuous SMF-stimulation could not affect the shape,distribution, and growth pattern of osteoblasts. The SMF of magnetic attachments could lead to certain changes in surface uhrastructures of osteoblasts in this study.

OBJECTIVETo investigate the expression of keratin 8 (K8) in carbon tetrachloride (CCl(4))-induced liver injury of mice.

METHODSForty ICR mice were divided into four groups. CCl(4) 300 microl/kg body weight in olive oil was injected intraperitoneally for 0, 2, 4, 6 weeks in group A, B, C and D, respectively. Mice were sacrificed 3 d after the last injection and then the vital organs were collected and weighed. RT-PCR and immunohistochemistry methods were used to analyze the expression of keratin 8 in the liver.

RESULTSThe ratios of liver and body weight were increased significantly after administration of CCl(4), which were 5.60 %, 6.87%, 7.83 % and 7.76% at 0, 2, 4 and 6 weeks after injection, respectively. The expression of K8 was increased at the 2 w, 4 w and 6 w after CL(4) administration.

CONCLUSIONThe expression of K8 is positively correlated with the liver injury induced by CCl(4). The accumulation of K8 may be involved in the mechanism of liver injury.

Animals ; Carbon Tetrachloride ; Chemical and Drug Induced Liver Injury ; metabolism ; Female ; Keratin-8 ; genetics ; metabolism ; Male ; Mice ; Mice, Inbred ICR ; RNA, Messenger ; genetics ; metabolism

OBJECTIVETo compare the different restorations of endodontically treated premolars, and to choose the best method.

METHODS10 pairs of symmetry maxillary first premolars were collected from 10 patients, and randomly divided into 2 groups. Every pair received different treatment accordingly. The test group was set up the medial-occlusal cavities model under the same standard. The 10 pairs teeth were test by instron 4302 universal test machine in speed of 1.00 mm/min until broken up and the top load was recorded. The load angle was 45 degree to the long axis of the teeth, as the load was pointed to the middle of the lingual surface on the buccal cusp. The records were analyzed by paired samples t test.

RESULTSBased on the medial-occlusal cavities, the fracture strength of the full crowned teeth was lower than the full natural teeth, and the fracture strength of the post-crowned teeth was higher than the full natural teeth.

CONCLUSIONFrom the point of biomechanics, the different trauma of the endodontically treated teeth fits to different restorations. The medial-occlusion cavities fit the post-crown restoration mostly.

Bicuspid ; Crowns ; Dental Caries ; Dental Stress Analysis ; Humans ; Tooth Fractures ; Tooth, Nonvital

OBJECTIVETo perform a Meta-analysis on peginterferon with interferon in treatment of HIV patients coinfected with refractory genotype HCV.

METHODSA literature search of Medline was conducted to identify eligible randomized controlled trials. Meta analysis was conducted to evaluate peginterferon and interferon in treatment of coinfected HCV genotype 1 or 4 in HIV patients.

RESULTSix trials of 88 matched the selection criteria. Total 1,131 patients with coinfection of HCV genotype 1 or 4 and HIV were included. Sustain viral response was higher in patients treated with peginterferon plus ribavirin compared with that of interferon plus ribavirin (26 % compared with 8 %) or peginterferon alone (26 % compared with 13 %). Severe adverse effects and withdrawal rates were similar for patients treated with peginterferon and patients treated with interferon.

CONCLUSIONPeginterferon plus ribavirin in treatment of patients with coinfection of genotype 1 or 4 HCV and HIV can achieve higher sustain viral response and the likelihoods of serious adverse effects and withdrawal rates are similar to other therapies.

Adult ; Antiviral Agents ; administration & dosage ; Drug Therapy, Combination ; Female ; Genotype ; HIV Infections ; complications ; drug therapy ; immunology ; Hepacivirus ; classification ; genetics ; Hepatitis C, Chronic ; complications ; drug therapy ; virology ; Humans ; Interferon-alpha ; administration & dosage ; Male ; Polyethylene Glycols ; administration & dosage ; Randomized Controlled Trials as Topic ; Recombinant Proteins ; Ribavirin ; administration & dosage