Adolescent ; Adult ; Child ; Dexamethasone ; administration & dosage ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Rhinitis, Allergic, Perennial ; therapy ; Volatilization

AIM:To observe the expression of Smad7 and Smad ubiquition regulatory factor-Smurf2 in rat glomerular mesangial cells (GMC) stimulated by the high concentration of glucose,and to investigate the effect of the ubiquition on Smad signaling by adding MG132 as a proteasome differential inhibitor. METHODS:Cultured rat GMC were divided into normal group (the concentration of glucose:5.6 mmol/L),high glucose group (20 mmol/L,30 mmol/L,respectively),therapy group (30 mmol/L glucose with MG132). The expressions of Smurf2 and Smad7 in each group were measured by indirect immunofluorescence and laser scanning confocal microscope. RESULTS:(1) The expression of Smurf2 in GMC in normal group was weak (25.93?3.35) whereas the expression of Smad7 was strong (64.09?7.43). (2) The expression of Smurf2 in high glucose group was stronger than that in normal group (P

Humans ; Lymphohistiocytosis, Hemophagocytic ; diagnosis ; therapy

Objective To observe the oral Simotang plus traditional Chinese medicine enema in treatment of postoperative adhesion intestinal obstruction in patients with colon cancer.Methods The clinical data of 45 colon cancer patients with postoperative adhesive intestinal obstruction was analyzed retrospectively.The patients were randomly divided into control group (22 cases) and treatment group (23 cases).The control group was given conventional treatment,the treatment group was treated with oral Simotang plus traditional Chinese medicine enema on the basis of conventional treatment.The clinical efficacy,traditional Chinese medicine syndrome integral,serum endothelin (ET),tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) and other indicators were compared between the two groups.Results After treatment,the total effective rates in control group and treatment group were 86.4 ％ (19/22) and 91.3 ％ (21/23),the difference was statistically significant (x2 =5.201,P =0.016).The operation rates in control group and treatment group were 13.6 ％ (3/22) and 8.7 ％ (2/23),the difference was statistically significant (x2 =3.622,P =0.027).The treatment group had shorter gastrointestinal recovery time and hospitalization time,the difference was statistically significant (both P ＜ 0.05).Before treatment,the traditional Chinese medicine syndrome integral in two groups had no significant difference (t =0.826,P ＞ 0.05),after treatment,the integral in treatment group was lower than that in control group (t =0.553,P ＜ 0.05).In treatment group,the differences of ET,TNF-α,IL-6 between 3,5,7 d and 1 d were statistically significant (all P ＜ 0.01),and compared with the control group at the same time,there were obvious advantages(all P ＜ 0.01).Conclusion The efficacy of oral Simotang and traditional Chinese medicine enema in treatment of adhesive intestinal obstruction after resection of colorectal cancer is clear,it is worthy of clinical application.

Cells, Cultured ; Coal ; toxicity ; Dust ; Humans ; In Vitro Techniques ; Mutagenicity Tests ; Mutagens ; toxicity ; Mutation ; drug effects ; Salmonella typhimurium ; drug effects ; genetics ; Sister Chromatid Exchange ; drug effects

Objective To sum up experience with surgical excision of isolated local recurrence for renal cell carcinoma. Methods From March 2004 to November 2007, 7 patients (five cases un-derwent radical nephrectomy and two nephron-sparing surgery) with isolated local recurrence of renal cell carcinoma were treated at our department. All patients underwent extensive surgery for local re-currence. Results The mean patient age was 42 years (range 19 to 6). The mean time to local re-currence was 23.3 months (range 12 to 54). The Mean size of the recurrent tumor was 5.2 cm(range 2.5 to 10.5). Peritoneal exploration was performed in 7 patients and 5 had complete en bloc excision of the renal cell carcinoma mass. 2 patients gross disease was excised. The mean blood loss was 1050 (150-3000) ml. Surgical complications occurred in 2 patients, iliohypogastric nerve injure in one and ileus performation in another one. All patients recovered finally. Six patients were followed and one lost follow-up. Mean follow-up time was 13(8-27) months. One patient died of metastatic disease at 22 months after excision of the renal cell carcinoma mass. Conelusion En bloc excision of isolated locally recurrent renal cell carcinoma is possible, and complete surgical resection could lead to pro-longed disease-free survival.

AIM: To study the antipyretic, analgesic and antiinflammatory actions of dexketoprofen.　METHODS: Hot-plate and tail-flick models in mice, fever model in rats and rabbits induced by carrageenin or typhoid vaccine respectively, ear swelling induced by dimethylbenzene in mice; foot pad swellings induced by carrageenin and granuloma induced by cotton in rats were adopted.　RESULTS: Dexketoprofen (7.5,15,30 mg*kg-1) could significantly prolong latency of analgesic in hot-plate and tail-flick models in mice and reduce carrageenin-induced fever in rats, and inhibit dimethylbenzene-induced ear swelling in mice. Dexketoprofen (1.9,3.8,7.5 mg*kg-1) could significantly inhibit typhoid vaccine-induced fever in rabbits; Dexketoprofen (3.8,7.5,15 mgkg-1) could also significantly inhibit the swelling of foot pad induced by carrageenin, and the granuloma induced by cotton in rats.　CONCLUSION: Dexketoprofen has antipyretic, analgetic and antiinflammatory effect.

Clinical laboratory information system is the key to the realization of total laboratory automation,standardization,intelligent and digitization.In recent years,with the establishment,application,popularization and upgrading of clinical laboratory information system,it is very important to establish the construction criterion of clinical laboratory as soon as possible.Now according to the requirements of construction and management of modern clinical laboratory and combining with the practical application of clinical laboratory information system in our hospital at domestic settings,the standardization construction of clinical laboratory information system was discussed in this article.

Objective To investigate the therapeutic effieacy and safety of 18 α-Diammonium glycyrrhizinate phosphatidylcholine complex (DGPC) in patients with chronic hepatitis B and or C with elevated aminotransferase. Methods 55 patients with chronic hepatitis B and or C, with serum alanine aminotransferase (ALT) of 2 to 10 times the upper limit of normal were randomly assigned to receive DGPC or Diammonium glyeyrrhizinate (DG) for 12 weeks. Then they were followed up for an additional 4 weeks. From week 1 to 10, DGPC or DG was given as 150 nag,three times a day (TID). At the 11th week,the drug was given as 100 mg,TID. Then 50 mg,TID for the 12th week. Results ALT was markedly decreased after receiving DGPC 4,8,12 weeks (P=0.00). ALT normalization rate at the end of therapy was similar (38.5% vs 34.5% ,P =0.76). Drug-related adverse events were similar. Conclusion DGPC can rapidly and safely decrease aminotransferase in patients with chronic viurs hepatitis.

Adolescent ; Child ; Child, Preschool ; Clinical Trials as Topic ; Flow Cytometry ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunophenotyping ; Leukemia, Myeloid, Acute ; diagnosis ; pathology ; therapy ; Neoplasm, Residual ; diagnosis ; pathology ; therapy ; Polymerase Chain Reaction ; Prognosis ; Recurrence ; Sensitivity and Specificity