Adolescent ; Adult ; Child ; Dexamethasone ; administration & dosage ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Rhinitis, Allergic, Perennial ; therapy ; Volatilization

AIM:To observe the expression of Smad7 and Smad ubiquition regulatory factor-Smurf2 in rat glomerular mesangial cells (GMC) stimulated by the high concentration of glucose,and to investigate the effect of the ubiquition on Smad signaling by adding MG132 as a proteasome differential inhibitor. METHODS:Cultured rat GMC were divided into normal group (the concentration of glucose:5.6 mmol/L),high glucose group (20 mmol/L,30 mmol/L,respectively),therapy group (30 mmol/L glucose with MG132). The expressions of Smurf2 and Smad7 in each group were measured by indirect immunofluorescence and laser scanning confocal microscope. RESULTS:(1) The expression of Smurf2 in GMC in normal group was weak (25.93?3.35) whereas the expression of Smad7 was strong (64.09?7.43). (2) The expression of Smurf2 in high glucose group was stronger than that in normal group (P

Humans ; Lymphohistiocytosis, Hemophagocytic ; diagnosis ; therapy

Objective To observe the oral Simotang plus traditional Chinese medicine enema in treatment of postoperative adhesion intestinal obstruction in patients with colon cancer.Methods The clinical data of 45 colon cancer patients with postoperative adhesive intestinal obstruction was analyzed retrospectively.The patients were randomly divided into control group (22 cases) and treatment group (23 cases).The control group was given conventional treatment,the treatment group was treated with oral Simotang plus traditional Chinese medicine enema on the basis of conventional treatment.The clinical efficacy,traditional Chinese medicine syndrome integral,serum endothelin (ET),tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) and other indicators were compared between the two groups.Results After treatment,the total effective rates in control group and treatment group were 86.4 ％ (19/22) and 91.3 ％ (21/23),the difference was statistically significant (x2 =5.201,P =0.016).The operation rates in control group and treatment group were 13.6 ％ (3/22) and 8.7 ％ (2/23),the difference was statistically significant (x2 =3.622,P =0.027).The treatment group had shorter gastrointestinal recovery time and hospitalization time,the difference was statistically significant (both P ＜ 0.05).Before treatment,the traditional Chinese medicine syndrome integral in two groups had no significant difference (t =0.826,P ＞ 0.05),after treatment,the integral in treatment group was lower than that in control group (t =0.553,P ＜ 0.05).In treatment group,the differences of ET,TNF-α,IL-6 between 3,5,7 d and 1 d were statistically significant (all P ＜ 0.01),and compared with the control group at the same time,there were obvious advantages(all P ＜ 0.01).Conclusion The efficacy of oral Simotang and traditional Chinese medicine enema in treatment of adhesive intestinal obstruction after resection of colorectal cancer is clear,it is worthy of clinical application.

Cells, Cultured ; Coal ; toxicity ; Dust ; Humans ; In Vitro Techniques ; Mutagenicity Tests ; Mutagens ; toxicity ; Mutation ; drug effects ; Salmonella typhimurium ; drug effects ; genetics ; Sister Chromatid Exchange ; drug effects

Objective To sum up experience with surgical excision of isolated local recurrence for renal cell carcinoma. Methods From March 2004 to November 2007, 7 patients (five cases un-derwent radical nephrectomy and two nephron-sparing surgery) with isolated local recurrence of renal cell carcinoma were treated at our department. All patients underwent extensive surgery for local re-currence. Results The mean patient age was 42 years (range 19 to 6). The mean time to local re-currence was 23.3 months (range 12 to 54). The Mean size of the recurrent tumor was 5.2 cm(range 2.5 to 10.5). Peritoneal exploration was performed in 7 patients and 5 had complete en bloc excision of the renal cell carcinoma mass. 2 patients gross disease was excised. The mean blood loss was 1050 (150-3000) ml. Surgical complications occurred in 2 patients, iliohypogastric nerve injure in one and ileus performation in another one. All patients recovered finally. Six patients were followed and one lost follow-up. Mean follow-up time was 13(8-27) months. One patient died of metastatic disease at 22 months after excision of the renal cell carcinoma mass. Conelusion En bloc excision of isolated locally recurrent renal cell carcinoma is possible, and complete surgical resection could lead to pro-longed disease-free survival.

Child ; Child, Preschool ; Craniocerebral Trauma ; etiology ; surgery ; Female ; Humans ; Male

AIM: To investigate the molecular mechanism by which the SARS-CoV S protein induces chemokine IP-10 in airway epithelial cells.METHODS: cDNA microarrays were used to screen the gene expression profiles of human bronchial epithelial cells(16HBEs) stimulated by SARS-CoV S protein.In addition,RT-PCR,EMSA,and Western blotting were performed to analyze the phosphorylation of JAK/STAT signal pathway.The changes of IRF-1 and IP10 gene expression and the influence by the corresponding inhibitors were analyzed.RESULTS: IRF-1,a key transcription factor of the JAK/STAT signal pathway,was activated in human bronchial epithelial cells after stimulation by the S protein of SARS-CoV.The IP-10 gene expression was detected 2 h following the phosphorylation of STAT1 after 15 min,which was blocked by STAT1or JAK2 inhibitors.Electrophoretic mobility shift assay(EMSA) demonstrated that the nuclear proteins bound to ISRE and GAS but not NF-?B DNA motif.CONCLUSION: The SARS-CoV S protein induces IP-10 gene expression in human bronchial epithelial cells through activation of the JAK/STAT signal pathway,suggesting that the JAK/STAT signal pathway activated by virus plays key roles in virus infection related acute lung injury.

AIM: To study the antipyretic, analgesic and antiinflammatory actions of dexketoprofen.　METHODS: Hot-plate and tail-flick models in mice, fever model in rats and rabbits induced by carrageenin or typhoid vaccine respectively, ear swelling induced by dimethylbenzene in mice; foot pad swellings induced by carrageenin and granuloma induced by cotton in rats were adopted.　RESULTS: Dexketoprofen (7.5,15,30 mg*kg-1) could significantly prolong latency of analgesic in hot-plate and tail-flick models in mice and reduce carrageenin-induced fever in rats, and inhibit dimethylbenzene-induced ear swelling in mice. Dexketoprofen (1.9,3.8,7.5 mg*kg-1) could significantly inhibit typhoid vaccine-induced fever in rabbits; Dexketoprofen (3.8,7.5,15 mgkg-1) could also significantly inhibit the swelling of foot pad induced by carrageenin, and the granuloma induced by cotton in rats.　CONCLUSION: Dexketoprofen has antipyretic, analgetic and antiinflammatory effect.

Objective To study and compare the protective effects of pmtease inhibitor and corticosteroid on endotoxin-indueed acute lung injury in order to guide the choice of appropriate drugs. Method Thirty-two New Zealand rabbits were randomly divided(random number) into four groups with 8 rabbits in each, namely normal controls(C) ; lipopolysaecharide(LPS) group(L) ; ulinastatin(UTI) group(U) and dexamcthasone(DEX) group (D) .Except group C, all rabbits were injected with a dose of LPS 600 μg/kg iv. Meanwhile the rabbits in group U,group D received UTI(100 000 μ/kg), DEX(5 mg/kg), respectively. The specimens were collected 4 hours later for detecting the levels of TNF-α and NO in serum, and blood gas analysis, histological manifestations, the lung wet/dry weight ratio, lung tissue MPO and SOD activity, lung tissue MDA. Data were analyzed by ANOVA (SNK- q test), and P < 0.05 was considered as significantly different. Results Compared with group C, the lungs of the rabbits in group L had inflammatory granulocyte infdtration, diffused alveolar septum thickening and hemorrhagic spots were observed in pathological examinations. The histological changes of group U and group D were much lessened than those in group L. As groups U and D were compared with group L, there were significant differences inmany biomarkers including lung wet/dry weight ratio[(5.02±0.11),(4.93±0.13) vs.(5.37 ±0.29)],lung tissue MPO activity[(0.51 ± 0.05),(0.54±0.07) vs.(0.82 ± 0.09)] and MDA[(0.82 ±0.05),(0.81 ±0.04) vs.(0.96±0.05)], NO[(296.2± 11.7),(291.7 ± 15.8) vs.(351.8±19.6)] and TNF-α[group D(2.021 ± 0.122) vs. group L(4.999 ± 0.139)],lung tissue SOD activity[(120.3 ± 6.1),(122.6±3.5) vs.(105.1 ± 8.5)] and blood gas analysis[pH(7.30±0.23),(7.30±0.17) vs.(7.22±0.45) and PaO_2( 101.9 ± 6.8).( 102.5 ± 4.7) vs.(80.3 ± 3.3)] ; but there were no differences of above mentioned biomarkers between group U and D( P > 0.05). And there were no significant differences in PaCO_2 betweeu group U and D and group L[(37.0 ± 3.3),(37.6 ± 3.0) vs.(34.8 ± 2.3)]( P > 0.05). Conclusions The protective effects of ulinastatin on endotoxin-induced acute lung injury is comparable to those of dexamethasone, thus the former may be a clinical substitute for the latter with less side effects.