OBJECTIVE: To identify and analyze the potential impurities of methocarbamol on the basis of its synthetic route and starting materials.

An analytical procedure for the determination of water-and methanol-extractable pentachlorophenol ( PCP) in soils using vortex assisted liquid-liquid extraction ( VALLE ) and gas chromatography ( GC ) was developed. Significant extraction parameters, such as liquid-liquid ratio and vortex speed were optimized. The recovery of PCP was the highest ( 97. 4%) at the vortex rotation speed of 2000 r/min, with good reproducbility and a small relative sdandard deviation ( RSD, 0. 5%), with regard to the volume ratio of derivatization solution to n-hexane, the recovery of PCP was 103% with an RSD of 0. 7% at 10:4. The linearity of the calibration ranged from 1. 25 μg/L to 4000 μg/L, with a correlation coefficient ( R2 ) of 0. 9999. The detection limit of the PCP in water was below 0. 2 μg/L. Compared with traditional liquid-liquid extraction ( LLE) and solid phase extraction ( SPE) , the VALLE method was more simple and more economic in terms of chemical consumption, also with a recovery of 96. 8% and a RSD of 3. 7%. Four different soils were used to check the reliability of this method. The soils were respectively extracted with water once and methanol three times. The total recoveries were 89. 5%-98. 9% and 88. 7%-98. 4% at the PCP spiking level of 1 and 10 mg/kg, respectively. It is concluded that the VALLE method satisfied the extraction and determination of water and methanol extractable PCP in soils with varying PCP polluting rates.

Objective To investigate the expressions of KLF2 mRNA and KLF4 mRNA in the acute lung injury (ALl) rats induced by lipopolysaccharide (LPS),and to analyze the correlation between KLF2,KLF4 and ALI.Methods A total of 100 SD rats were randomly divided into 2 groups:normal control group and LPS treated group,then the latter group was randomly further divided into 3 subgroups as per the serum and lung tissue samples taken separately at 2,4 and 24h after modeling.The ALI model was made by injecting 5mg/kg LPS into tail vein.The pathological changes of lung tissue were observed in each group,and the expressions of KLF2,KLF4 mRNA in serum and lung tissue were detected by RT-PCR.The data of laboratory findings were analyzed with SPSS 17.0 software for statistical analysis.Results The histopathological changes showed the most obvious damage of lung tissue occurred at 4 hours after modeling.The expressions of KLF2 mRNA and KLF4 mRNA in the lung tissue and serum of control group were significantly higher compared to LPS treated subgroups (P ＜0.01).The expression of KLF2 mRNA in LPS treated subgroup at 2 hours was lower than that in LPS subgroups at 4 hours and 24 hours (P ＜ 0.01),while the expression of KLF4 mRNA in LPS treated subgroup at 4 hours was lower than that in LPS treated subgroups at 2 hours and 24 hours (P ＜ 0.01).Conclusions The expression of KLF2 mRNA was occurred earlier than the pathological changes in acute lung injury,while the expression of KLF4 was emerged synchronously,and both KLF2 and KLF4 could be used as candidates of predictive and diagnostics molecular markers of ALI.

Aim To explore the effects of the Melittin on growth and cell cycle of SGC-7901 cells.Methods Growth inhibition effect of Melittin was evaluated using SRB in SGC-7901 cells in vitro;Melittin induced cell cycle arrest was investigated using flow cytometry assay;reverse transcription PCR(RT-PCR)was used to detect the associated protein mRNA of cell cycle.Results Proliferation activity of SGC-7901 cells was inhibited after treatment with Melittin(1,2,4,8,16,32×10~(-3) μg·L~(-1))(P<0.05 or P<0.01)for 24 h;Flowcytometry analysis revealed that SGC-7901 cells accumulated in the G_2/M phase after treatment with Melittin(4,8×10~(-3) μg·L~(-1))for 24 h;the expression of CylinB1,CDK1 and Cdc25c mRNA were decreased.Conclutions Proliferation activity of SGC-7901 cells was inhibited by Melittin,which may be related to the inhibitory effect of Melittin on associated protein transcription in the G_2/M stage of SGC-7901 cells.

Aim To evaluate the anti-tumor activity of the extract from the root of Actinidia chinensis planch in vitro and in vivo. Methods Active components from the root of Actinidia chinensis planch were isolated by traditional phytochemical techniques. The in vitro anti-tumor activity was determined by sulforhodamine B assay and the in vivo anti-tumor activity was evaluated using experimental mouse tumor models and human tumor xenografts in nude mice. Results Powdered air-dried roots of Actinidia chinensis planch were percolated with methanol at room temperature thrice. The filtrate was concentrated to dryness in vacuo and then was further extracted with ethyl acetate, n-butanol , and chloroform. The fraction extracted by chloroform displayed the most potent activity against several tumor cell lines including hepatocellular carcinoma Bel-7402 cells, non-small cell lung cancer A549 cells, lymphoma Ramos cells, and breast cancer MCF-7 cells. Further more, the anti-tumor efficacy of the chloroform fraction was confirmed in Bel-7402 xenografts in nude mice with the percentage inhibition of 38.0 %. Conclusion The extract of the root of Actinidia chinensis planch has anti-tumor activity, and the active components are mainly in the fraction extracted by chloroform.

OBJECTIVETo analyze the infection of human parvovirus B19, human bocavirus (HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.

METHODSNested PCR assays were designed and validated to detect B19, HBoV and PARV4, respectively. The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing. The parvovirus infection was analyzed statistically.

RESULTSThe detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed. The frequency of B19 and HBoV were 2/95 (2.1%) and 9/95 (9.5%) in blood samples respectively. No PARV4 was detected. HBoV was detected in 3/5 patients with drug-induced hepatitis.

CONCLUSIONBoth B19 and HBoV infection were detected in blood from patients with liver disease.

Adolescent ; Adult ; Aged ; Child ; Coinfection ; virology ; Female ; Human bocavirus ; isolation & purification ; Humans ; Liver Diseases ; virology ; Male ; Middle Aged ; Parvovirus ; isolation & purification ; Parvovirus B19, Human ; isolation & purification ; Polymerase Chain Reaction ; Viremia ; virology

OBJECTIVETo study the color changes on brightness and hue in dental ceramic prothesis materials.

METHODSThe different color mark of VINTAGE series was sintered on 15 pieces Ni-Cr alloy denture materials. The sample group and control group was given the sintering of color blending with equivalent volume. The order of color mark was of A1 and A2, A2 and A3, A3 and A4, B1 and B2, B2 and B3, B3 and B4 in sample group, and A1, A2, A3, A3.5, A4, B1, B2, B3 and B4 orderly in control group. The chromatic analysis on samples of VITA had been taken through applying PR 650 spectrum detector and adopted the statistic software of Photo Research and SPSS 12.0 for analysis.

RESULTSThe statistical difference (P<0.05) of L*a*b* value was found in the different color mark of the same color serials. A non-linearity relation had been found in the chromatic parameters a*b*. It means the change of color was visible.

CONCLUSIONSet A has a bigger grading change of brightness than Set B. The change of red-green is obviously in Set A, as well as yellow-blue in Set B.

Ceramics ; Color ; Dental Materials ; Humans

OBJECTIVETo study the effect existed in the course of adopting different thickness and chromatics of various opaques so that a good methods can be provided for clinical care in prosthodontics.

METHODSA model of using the thin plastic paper to control the ceramic thickness was applied in this test. The data of chromaticity was measured by TC-PIIG auto color difference meter and its results was processed by computer.

RESULTSThe value of brightness had an uptrend increase with augment of opaques thickness. Metal base color was opaqued and the basic hue was set. A lower and unstable value, besides a relative stable Y, was measured with the uncompleted opaquing. A stability of hue had been gradually concentrated within a certain range accompanying with the increase of opaquing ceramic thickness. A concentrated hue in the second thickness was found in CARAT ceramic which had high ability of opaque and had a stable hue of ceramic in comparison with other opaques. Among those changes, CARAT's brightness showed a minimal change; a gradual stability of chromatics was presented and was limited with-in a certain range though the augment of opaques.

CONCLUSIONThe metal color in base could be completely opaqued while opaques thickness reaches a certain range. CARAT is the materials of minimal thickness and has relatively strong capacity for opaques.

Ceramics ; Color ; Dental Alloys ; Dental Porcelain ; Materials Testing ; Metal Ceramic Alloys

A phytochemical investigation on the stems of Garcinia bracteata collected from Xishuangbanna resulted in the isolation of a new flavone. By analysis of the HRESIMS, IR, UV, 1D and 2D NMR spectra, the structure of the new compound was determined as 7-methoxy-4',6-dihydroxy-8-isobutyryl-flavone(1). Compound 1 was also tested for its anti-tobacco mosaic virus(TMV) activity. Results suggested the 1 possessed remarkable anti-TMV activity, with an inhibition rate of 28.2%.
Antiviral Agents ; chemistry ; isolation & purification ; pharmacology ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; pharmacology ; Flavones ; chemistry ; isolation & purification ; pharmacology ; Garcinia ; chemistry ; Magnetic Resonance Spectroscopy ; Plant Leaves ; chemistry ; Tobacco Mosaic Virus ; drug effects ; growth & development

OBJECTIVETo observe the therapeutic efficacy of IFN or oxymatrine in combination with lamivudine in patients with lamivudine-resistant chronic hepatitis B.

METHODSForty patients ongoing treatment with lamivudine were randomized to three groups: group A, 14 patients with addition of IFN alpha-2b 3MU to ongoing lamivudine, daily, one month, followed by the same dose given every other day, five months; group B, 15 patients with addition of injectable oxymatrine 60 mg daily, three months, followed by oral oxymatrine every day, three months, and group C, 11 patients ongoing treatment with lamivudine alone. The HBV DNA level in serum, HBeAg seroconversion, and ALT level were detected at the end of the treatment.

RESULTSAfter 6 months of treatment, HBV DNA became negative in 35.73% patients treated with combination with IFN, and in 13.3% patients treated with combination with oxymatrine. ALT level was normal in 85.71% or 86.66% of patients, respectively. In none of the patients under ongoing treatment with lamivudine alone HBV DNA or HBeAg became negative, and ALT level was normal in 36.36% of patients.

CONCLUSIONThese data indicated that IFN or oxymatrine in combination with ongoing lamivudine therapy provided effective antiviral therapy in patients with lamivudine-resistant HBV. The addition of IFN or oxymatrine to ongoing lamivudine therapy in lamivudine-resistant patients led to significant inhibition of viral replication and improvement in liver function after 6 months of therapy.

Adult ; Alkaloids ; administration & dosage ; Antiviral Agents ; administration & dosage ; Drug Resistance, Viral ; Drug Therapy, Combination ; Female ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; drug therapy ; pathology ; Humans ; Interferon-alpha ; administration & dosage ; Lamivudine ; pharmacology ; Male ; Middle Aged ; Quinolizines ; Recombinant Proteins ; Treatment Outcome