Objective　The poststroke depression(PSD) is a common complication in the cerebrovascular diseases.Methods　Having used the monoamine oxidase inhibitor,procaine hydrochloride composite film-coating tablets,we observed the Hamilton rating scale for depression(HAMD) and selfrating depression(SDS) of the patients with poststroke depression.Results　Two weeks late after the procaine hydrochloride composite film-coating tablets administrated,the scales of HAMD and SDS have been improved obviously.Conclusion　This investigation has suggested that the monoamine oxidase inhibitor can improve the depression of the patients with poststroke and be beneficial to the brain stroke.

In order to increase the underachievement students’ score,we investigate their way of learning by questionnaire and interview. We find that learning motivation,learning style,learning time and thinking ability are the most important factors. So the underachievement students can be divided into four classes: learning motivation insufficiency, learning method incorrectness, learning time insufficiency and thinking ability insufficiency. And we raise some education strategies for each class.

Objective To investigate the effects of exercise training intervention on the expressions of TNF-α and adiponectin in circulation and tissues of high-fat/high-sucrose diet-induced insulin resistance rats. Methods 29 S-D rats were random divided into 2 groups: Control group (9 rats) and high-fat/high-sucrose diet group (20 rats). After fed for 6 weeks, 18 rats with insulin resistance were random divided into 2 groups: model group ( n = 9) and exercise group ( n = 9). After 6 weeks intervention, serum TNF-α and adiponectin concentration were measured by radioimmunity assay and ELISA respectively, while TNF-α and adiponectin mRNA expressions in liver and skeletal muscle were measured by RT-PCR. Results Fasting plasma glucose(FPG) and fasting serum insulin(FINS) levels increased significantly and insulin sensitivity index(ISI) decreased significantly in rats of model group than those in control group(7.49 ± 1.13 vs 5.06±0.38, 33.57 ±4.87 vs 13.61±2.94, -5.51±0.16 vs -4.21 ±0.22, all P <0.05). Serum TNF-α concentration was significantly higher than those in control group, while serum adiponectin concentration was significantly lower. (3.03 ± 0. 50 vs 2. 39 ± 0. 44, 0. 77 ± 0. 09 vs 0. 86 ± 0. 08, all P < 0. 05 ).Expressions of TNF-α mRNA in liver and skeletal muscle increased significantly and adiponectin mRNA expression significantly decreased in rats of model group compared to those in control group (0. 66 ± 0. 19 vs 0. 05 ± 0. 03, 1.15 ± 0. 20 vs 0. 25 ± 0. 10, 0. 25 ± 0. 10 vs 0. 85 ± 0. 13, all P < 0. 01 ). Fasting plasma glucose(FPG) and fasting serum insulin(FINS) levels decreased significantly and insulin sensitivity index (ISI) increased significantly in rats of exercise group than those in model group(5.77 ± 1.17 vs 7.49 ±1.13, 25.69 ±4.27 vs 33.57 ±4. 87, -5. 10 ±0.31 vs -5.51 ±0. 16, all P <0.05) ;Serum TNF-α concentration was significantly lower and serum adiponectin concentration was significantly higher in rats of exercise group than those in model group ( 2.40 ± 0. 59 vs 3.03 ± 0. 50, 0. 86 ± 0. 10 vs 0. 77 ± 0. 09, all P < 0. 05); Expressions of TNF-α mRNA in liver and skeletal muscle decreased significantly and adiponectin mRNA expression increased significantly in rats of exercise group compared to those in model group (0. 21±0. 10 vs 0.66±0. 19, 0.49 ±0. 17 vs 1.15 ±0.20, 0.97 ±0.20 vs 0. 25 ±0. 10, all P <0.01). Conclusion Exercise training can significantly improve insulin resistance, which may be through modulating the expressions of TNF-α and adiponectin.

Precise verification is required in the execution of intensity-modulated radiotherapy (IMRT) for its complexity. Thereinto, the verification of dose distributions is the emphases and nodus. Some problems in the verification by using radiotherapy planning dose verification system (DVS) are explored such as how to verify the precision and credibility, how to reduce errors in every verification step, how to provide quantified analysis. A suit of practical methods and techniques are summarized from equipment selection to quantified analysis.

Objective To evaluate the effectiveness and safety of one stage posterior vertebral osteotomy for correction of severe and rigid congenital scoliosis associated with Ⅰ, Ⅱ type of diastematomyelia.Methods According to the diastematomyelia packet,52 patients were divided into type Ⅰ group performed with mediastinum resection combined with spinal osteotomy, group Ⅱ without treatment of diastematomyelia direct spinal osteotomy.Group Ⅲ spinal osteotomy directly without diastematomyelia.Results The mean operation time was (548.6±113.2) min,the average amount of bleeding was (3 728.6±1 436.5) ml.In group Ⅰ,the mean operation time was (608.6± 123.2) min, significantly longer than those of group Ⅱ ((521.3 ±102.4) min,t=2.787,P＜0.01).In group Ⅰ the average amount of bleeding was (5 018.3 ±2 174.2) ml, significant more than that of group Ⅱ((2 615.3± 1 132.8) ml,t=5.182,P＜0.01).Patients with preoperative Cobb angle measurement for (95.2± 14.3) degrees, postoperative for (35.2± 14.8) degrees, follow-up of 2 years for (37.6± 16.1) degrees, group Ⅰ included preoperative (92.3 ± 12.8) degrees, postoperative (32.6 ± 15.8)degrees, 2 years later (35.8 ± 17.2) degrees;group Ⅱ before operation (99.2 ± 17.3) degrees, postoperative (37.3±14.3)degrees, 2 years later (40.2± 15.3) degrees.The postoperative Cobb angle correction rate and correction loss rate showed no significant difference between two groups (P ＞0.05), a posterior spinal osteotomy for the treatment of type Ⅰ and type Ⅱ with diastematomyelia severe rigid congenital scoliosis has good correction effect.This group of patients, the complication rate was 21.2％ (11/52);where in Ⅰ group the incidence rate of 36.4％ (8/22) was significantly higher than that of Ⅱ group 10.0％ (3/30) (P =0.021).Conclusion One stage posterior vertebral osteotomy for severe rigid with diastematomyelia of congenital scoliosis with the feasibility, effectiveness and safety, patients with type Ⅰ diastematomyelia should first bony mediastinum resection, Ⅱ type of diastematomyelia there is no need for treatment of diastematomyelia.

Objective To evaluate the effect of phosphatidylinositol-3 kinase inhibitor LY294002 combined with dichloroacetate on apoptosis in human pulmonary arterial smooth muscle cells (SMCs) and AKT/GSK-3β/HK-2 signaling pathway.Methods Human pulmonary arterial SMCs were seeded into culture plates at a density of 2 x 104 cells/ml after 3-5 passages.After being incubated for 72 h,the SMCs were cultured in the medium supplemented with 0.2％ fetal bovine serum for 24 h to induce starvation prior to experiments.The cells were then randomly divided into 6 groups (n =6 each) using a random number table:control group (group C),positive control group (group F),LY294002 group (group L),different concentrations of dichloroacetate groups (D1 and D2 groups),and LY294002 combined with dichloroacetate group (group LD1).In group C,the cells were cultured in the medium supplemented with 0.2％ fetal bovine serum.In F,L,D1,D2 and LD1 groups,the cells were cultured in the medium supplemented with 10％ fetal bovine serum.LY294002 2 μmol/L was added to the medium in group L.Dichloroacetate l0 and 20 mmol/L were added to the medium in D1 and D2 groups,respectively.In group LD1,LY294002 (2 μmol/L) was added,and 30 min later dichloroacetate 10 mmol/L was added to the medium in LD1 group.The cells were incubated for 48 h.Flow cytometry was used to measure the cell apoptosis and mitochondrial membrane potential.The expression of phosphorylated AKT (p-Akt),phosphorylated glycogen synthase kinase 3β (p-GSK-3β),and hexokinase-2 (HK-2) was detected using Western blot.Apoptosis rate was calculated.Results Compared with group C,apoptosis rate was significantly increased,and mitochondrial membrane potential was decreased in D2 and LD1 groups,the expression of p-Akt,p-GSK-3β and HK-2 was up-regulated in group F,and no significant changes were found in apoptosis rate and mitochondrial membrane potential in F,L and D1 groups.Compared with group D2,apoptosis rate was significantly increased,mitochondrial membrane potential was decreased,and the expression of p-Akt,p-GSK-3β and HK-2 was down-regulated in LD1 group.The expression of p-Akt,p-GSK-3β and HK-2 was significantly lower in D2 and LD1 groups than in group F.Conclusion LY294002 combined with dichloroacetate can promote apoptosis in human pulmonary arterial SMCs possibly through blocking AKT/GSK-3β/HK-2 signaling pathway.

Objective To treat cerebrovascular stenosis with percutaneous transluminal angioplasty and stenting and analyze the problems of this method and its therapeutic effects.Methods Twenty-three patients with brain watershed infarction proven by CT or MRI because of severe cerebrovascular stenosis were investigated for their clinic features,cerebrovascular digital subtraction angiography(DSA) and neurologic status before operation and after stent placement.Results The offending arteries concerning carotid sinus(C1 segment) in 14 cases,internal carotid artery(C2 segment) in 2 cases,internal carotid artery(C5,6 segment) in 4 cases;The offending arteries involving left artery in 8 cases,right in 15 cases.The percutaneous transluminal angioplasty and stenting for all patients was successful.All patients did not suffer from TIA and stroke in 6-to 12-month follow-up.Conclusion The brain watershed infarction caused by atherosclerotic cerebrovascular stenosis was not an infrequent ischemic cerebrovascular disease.The percutaneous transluminal angioplasty and stenting is an effective treatment for carotid artery or internal carotid artery stenosis.With proper selection of the patients and meticulous technique,percutaneous transluminal angioplasty and stenting should be of safety and efficacy for stroke prevention and treatment.

Objective:To detect the gene mutation,protein expression of IL-2R? of TIL-T and the pr oliferation of TIL-T with the present of rIL-2 in B-NHL. Methods:The gene mutation of IL-2R? was performed on 19 TIL-T by PCR-SSCP;proliferat ion assay of 17 TIL-T with the rIL-2 was tests by MTT;IL-2R? protein express ion in cryostat section of 29 B-NHL were determined by immunohistochemical stai n. Results:SSCP showed there is no mutation happened in the cDNA of IL-2R? of TIL-T.Pro liferation test showed the intensity of response of TIL-T was decreased in 76. 5% TIL-T(13 of 17 cases).The expression of CD25 protein in 86.2%(25 of 29 c ases) of B-NHL cases were (+) or (+/-). Conclusion:No genetic mutation had been found in IL-2R? of TIL-T,but IL-2R? protein i s weakly expressed in B-NHL;It indicated that there may be abnormal in the mech anism of activation of TIL-T by cell-cell contact. [

AIM: To observe the effects of valsartan on the cardiac hypertrophy and the expression of PTEN in spontaneously hypertensive rats (SHR). METHODS: Twenty 12-week-old male SHR were randomly divided into 2 groups:SHR positive control group and valsartan treating group ( 30 mg?kg -1?d -1). 10 homogenous Wistar-kyoto (WKY) rats were served as normal control group. Blood pressure and the ratio of left ventricular weight to body weight(LVW/BW)of rats were monitored periodically during the 8-weeks studies. Expression of PTEN in cardiac myocytes was determined by immunohistochemistry. RESULTS: Blood pressure and LVW/BW increased in the valsartan group more than those in the SHR control group, and the expression of PTEN in cardiac myocytes in valsartan group increased more than that in the SHR control group, but the indexes were lower than those in the WKY group. CONCLUSION: Valsartan can not only inhibit the progression of cardiac hypertrophy in spontaneously hypertensive rats, but also increase the expression of PTEN. PTEN may play a role in cardiac hypertrophy.

Carcinoma, Non-Small-Cell Lung ; genetics ; metabolism ; pathology ; DNA Mutational Analysis ; DNA, Neoplasm ; genetics ; isolation & purification ; Genes, erbB-1 ; Humans ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Mutation ; Paraffin Embedding ; methods ; Polymerase Chain Reaction ; methods ; Real-Time Polymerase Chain Reaction ; methods ; Receptor, Epidermal Growth Factor ; genetics