1.Effects of total flavonoid of chrysanthemum on Fas and Fasl expression in lacrimal gland cells of male castrated rabbits with dry eye
Xiao-Lei, YAO ; Qing-Hua, PENG ; Qi-Lei, CHEN ; Yong-Hua, TANG ; Qian, ZHONG
International Eye Science 2014;(10):1749-1754
AIM: To evaluate the effects of total flavonoid of chrysanthemum on the expressions of Fas and FasL in male rabbits with dry eye, and to investigate the therapeutic effects of the total flavonoid of chrysanthemum on dry eye.
METHODS: Totally 150 male Japanses white rabbits were divided into blank group ( group A ) , sham -operated group ( group B ) , model group ( group C ) , androgen control treatment group (group D), and total flavonoid of chrysanthemum treatment group ( group E ) . The dry eye model was established with orchiectomy on group C, D and E. Rabbits in group E were treated with total flavonoid of chrysanthemum. Rabbits in group D were treated with androgen intramuscular injection. Rabbits in the group A, group B, group C was treated with normal saline. All rabbits were detected with Schirmer's Ⅰ test and tear break-up time (BUT). Fas, FasL were checked on immunohistochemistry.
RESULTS:The Schirmer's I test values of group E was significantly higher than that of group C ( P<0. 01 ) and the BUT value of group E was significantly longer than that of group C ( P<0. 01 ). The quantity of positive expression of Fas in glandular tube cell and acinar epithelial celland apoptosis cells of group E after treatment at 1, 3, 5mo were significantly lower than that of group C, cell population of the positive expression of FasL was obviously higher than that of group C (P<0. 01).
CONCLUSION:The main component of chrysanthemum is flavonoid, which could significantly inhibit happening of dry eye in rabbit after androgen level lowered and lacrimal gland apoptosis and keep basic tears secretory volume and tear film stability.
2.Hepatic VX2 tumor after portal vein occlusion in rabbits:evaluation with DSA
Yue-Yong QI ; Li-Guang ZOU ; Shu-Hua DAI ; Xiao-Bing HUANG ; Ke-Qiang HAN ; Qi-Chuan ZHANG ; Lin CHEN ;
Journal of Interventional Radiology 2006;0(11):-
Objective To study the value of DSA for hepatic vascular anatomy,and to evaluate the efficacy of portal vein occlusion in rabbits with hepatic VX2 tumor.Methods Twenty New Zealand white rabbits were randomly divided into two groups with 10 in each group,including test group A and positive control group B of ham operation.For the test group A,portal branch ligation(PBL)was performed for the left external branch after 3 weeks of the tumor implantation to the left external lobe.Two weeks later,the DSA of hepatic artery and portal vein were performed in all of the rabbits.Results The total displaying effectiveness of the branches of hepatic artery by DSA was better than that by vascular perfusion.There was hypovascular blood supply to hepatic artery implantation of the tumor in the test group A,comparing with that of the group B.Conclusion DSA can clearly display spacial details of the hepatic vascular anatomy in rabbits,and play an important role in post-procedual evaluation of the portal vein occlusion in rabbits.
3.Phenolic glycosides from the stems and twigs of Strychnos cathayensis and their biological activities
Qi-ming PAN ; Shuang-gang MA ; Yong LI ; Dan ZHANG ; Hua SUN ; Qi HOU ; Xiao-guang CHEN ; Shi-shan YU
Acta Pharmaceutica Sinica 2023;57(1):170-179
Fourteen compounds were isolated from the
4.Research about effect of spray drying conditions on hygroscopicity of spray dry powder of gubi compound's water extract and its mechanism.
Jie ZONG ; Qi SHAO ; Hong-Qing ZHANG ; Yong-Lan PAN ; Hua-Xu ZHU ; Li-Wei GUO
China Journal of Chinese Materia Medica 2014;39(4):663-668
OBJECTIVETo investigate moisture content and hygroscopicity of spray dry powder of Gubi compound's water extract obtained at different spray drying conditions and laying a foundation for spray drying process of Chinese herbal compound preparation.
METHODIn the paper, on the basis of single-factor experiments, the author choose inlet temperature, liquid density, feed rate, air flow rate as investigated factors.
RESULTThe experimental absorption rate-time curve and scanning electron microscopy results showed that under different spray drying conditions the spray-dried powders have different morphology and different adsorption process.
CONCLUSIONAt different spray-dried conditions, the morphology and water content of the powder is different, these differences lead to differences in the adsorption process, at the appropriate inlet temperature and feed rate with a higher sample density and lower air flow rate, in the experimental system the optimum conditions is inlet temperature of 150 degrees C, feed density of 1.05 g x mL(-1), feed rate of 20 mL x min(-1) air flow rate of 30 m3 x h(-1).
Desiccation ; methods ; Drugs, Chinese Herbal ; chemistry ; Hydrophobic and Hydrophilic Interactions ; Particle Size ; Powders ; chemistry ; Temperature ; Water ; analysis ; Wettability
5.Balance of Treg/Th17 cells and the expression of related transcription factors in peripheral blood of patients with condyloma acuminatum
Hua CHEN ; Qi XIONG ; Yong-Yan CHEN
Chinese Journal of Infection Control 2017;16(12):1120-1125
Objective To evaluate the role and significance of Treg/Th17 cells imbalance in pathogenesis and recurrence mechanism of condyloma acuminatum (CA).Methods 52 patients with CA were selected as study group (CA group,27 with initial occurrence of CA,25 with recurrence of CA),30 healthy persons were as control group,flow cytometry was used to detect the proportion of Treg cells and Th17 cells in the peripheral blood,the expression level of Foxp3 mRNA and RORyt mRNA in peripheral blood mononuclear cells was determined by real-time quantitative polymerase chain reaction.Results The proportion of Treg cells and the expression level of Foxp3 mRNA in peripheral blood in CA group was higher than that in control group,recurrence CA group was higher than initial occurrence CA group,difference was significant(both P<0.05);the proportion of Th17 cells and expression level of RORyt mRNA in CA group was significantly lower than that in control group,proportion of Th17 cells in recurrence CA group was lower than initial occurrence CA group,there was significant difference (both P<0.05).The proporation of Treg/Th17 in CA group was higher than that in healthy controls(4.60[3.20,8.68] vs 1.39[1.05,2.05],P<0.05),recurrence CA group was higher than initial occurrence CA group (8.19[4.21,10.81] vs 3.52 [2.47,4.85],P<0.05).Conclusion There is an imbalance between Treg cells and Th17 cells in patients with CA,especially in patients with reccurrence of CA,the imbalance of Treg/Th17 cells may play an important role in the pathogenesis and recurrence mechanism of CA.
6.Separation and Preliminary Identification of Spoilage Organisms in Transmutative Soy Milk
Li-Ping WANG ; Qin-Hua ZHANG ; Yong ZHAO ; You-Rong CHEN ; Feng-Lan QI ; Wen ZHANG ;
Microbiology 1992;0(04):-
In this paper, three spoilage organisms were separated from five transmutative soy milks, and all the three spoilage bacteria could survive condition of both 1?105Pa,30min and 300mg/kg Nisin. Morpha character, physiological and biochemical characteristics, and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis, Bacillus pumilus and Brevibacillus borstelensis respectively. GenBank accessions for these three strains are EF439666-EF439668。
7.Etiological analysis of spontaneous sub-cortical hemorrhage
Xue-Min LI ; Qi-Zhong LUO ; Zhi-Min FEI ; Guo-Wei SHU ; Hua CUI ; Yong WANG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(09):-
Objective To discuss the frequent etiology of spontaneous sub-cortical hemorrhage and its diag- nosis.Methods The clinical materials of 79 cases of spontaneous sub-cortical hemorrhage were analyzed.Results 56% of the hemorrhage was caused by arterial-venous malformation.48% of the hemorrhage was caused by occult AVM.Conclusion AVM is the most frequent etiology of spontaneous sub-cortical hemorrhage.CTA plays a special role in its diagnosis.
8.Analysis and follow up research of 74 cases without psychosis in forensic psychiatric expertise.
Journal of Forensic Medicine 2006;22(2):135-136
OBJECTIVE:
To explore the characteristics and return of cases without psychosis in forensic psychiatry.
METHODS:
Seventy four cases without psychosis were follow up researched,and statistics relevant data.
RESULTS:
Five point seven percent cases were diagnosed without psychosis. They had explicit motive and purpose, protect by one's own was good, "the psychotic symptom" disappears after authenticate.
CONCLUSION
Analysis of many factor contribute to authenticate cases without psychosis. That schizophrenia symptom in early days should be identified to prevent from the misdiagnosis.
Adolescent
;
Adult
;
Crime
;
Expert Testimony
;
Female
;
Follow-Up Studies
;
Forensic Psychiatry
;
Humans
;
Male
;
Middle Aged
;
Psychotic Disorders/psychology*
;
Schizophrenic Psychology
;
Young Adult
9.An experimental study of the regional load deflection rate of multiloop edgewise arch wire in two dimension brackets.
Qi-shun LI ; Zhi-hua LI ; Jian-yong WU
West China Journal of Stomatology 2008;26(5):486-489
OBJECTIVETo compare the regional load deflection rate (LDR) of multiloop edgewise arch wire (MEAW) of three dimensions with coupled use of two dimension brackets in the individual interbracket span, to understand the mechanical properties of MEAW.
METHODSThe MEAW arch wires of stainless steel of three dimensions, 0.41 mm x 0.56 mm, 0.43 mm x 0.64 mm and 0.46 mm x 0.64 mm, were bent into single L-loop. The study was performed with the coupled use of 0.41 mm x 0.56 mm wires with 0.46 mm x 0.64 mm bracket (A bracket) and 0.43 mm x 0.64 mm, 0.46 mm x 0.64 mm wires with 0.56 mm x 0.71 mm bracket (B bracket). The LDR of each L-loop at the individual interbracket span when loading and unloading was measured. The data were analysed by SPSS 11.0.
RESULTSCompared the regional LDR for the couple of 0.41 mm x 0.56 mm L-loop with A bracket with those for the couple of 0.43 mm x 0.64 mm L-loop with B bracket, the former showed lower value than the latter at regions between the upper central and lateral incisor, the lower central and lateral incisor, and between the lower lateral incisor and canine (P < 0.05). For the rest regions, the two couples exhibited similar value to the regional LDR (P > 0.05). The regional LDR for the couple of 0.41 mm x 0.56 mm L-loop with A bracket were lower at all regions than those for the couple of 0.46 mm x 0.64 mm L-loop with B bracket (P < 0.05) except that at the region between the lower first and second molars which showed similar value between the two couples.
CONCLUSIONThe coupled use of B bracket with 0.43 mm x 0.64 mm MEAW arch wire and A bracket with 0.41 mm x 0.56 mm MEAW arch wire exhibited similar mechanical properties.
Humans ; Molar ; Orthodontic Brackets ; Orthodontic Wires ; Stainless Steel