OBJECTIVETo explore the alterations of after depolarization and triggered activity in myocardiac muscle during progression of mouse aortic stenosis.

METHODSAfter the establishment of mouse aortic stenosis model, the heart was collected and intracellular potential was recorded using standard glass microelectrode. After the recording, the action potential (AP), early after depolarization (EAD), delayed after depolarization (DAD) in papillary muscles were analysed.

RESULTS(1) Compared with that of time-matched control mice, there were no changes of the action potoutial duration at 90% repolarization(APD90) in model mice at 2 and 5 weeks, whereas the APD90 was progressively prolonged at 9 and 13 weeks. (2) During the 30 minites of the experiment, spontaneous EADs and DADs were frequently present in medel mice at 9 and 13 weeks, were not in control and model mice at 2 and 5 weeks. (3) Compared with those of control group, EADs and DADs triggered by hypokalaemia or isoproterenol were increased significantly. The incidence rate of triggering EADs and DADs was progressively increased over time.

CONCLUSIONEAD, DAD and trigger activities increase gradually during the process of aortic stenosis, and it suggests that the myocardium electrical-instability is markedly increased in the insult hearts.

Action Potentials ; physiology ; Animals ; Aortic Valve Stenosis ; physiopathology ; Disease Models, Animal ; Male ; Mice ; Myocytes, Cardiac ; physiology

OBJECTIVETo find the characteristic parameters from electrocardiogram (ECG) which is most related to pathological progress, surface ECG was performed in mice at 2, 5, 9 and 13 week post surgery.

METHODSElectrocardiogram recordings: The mice were anaesthetized with tribromoethanol (250 mg/kg, i.p.). Lead II surface ECG were acquired by using the Biopac System MP150 at a rate of 5 kHz.

RESULTS(1) No arrhythmia was observed in Sham and 2-week Band mice. Spontaneous arrhythmias were observed in ECG recordings, with an incidence of 15%, 28% and 63% in 5-, 9- and 13-week Band mice, respectively. Tachyarrhythmias, such as frequent premature ventricular extrasystole, bigeminy, trigeminy, and paroxysmal tachycardia were found. (2) Analysis of ECG recordings revealed a significant prolongation of QT and QTc intervals. Compared with age-matched Sham mice, the increment in QT and QTc intervals was 20.4%, 32.7%, 49.7%, 61.0% and 27.1%, 32.1%, 43.9%, 59.1% at 2, 5, 9 and 13 week, respectively (P < 0.01). (3) The characteristic change in electrocardiogram was on the J wave. The amplitude of J wave was upward in Sham mice, and it was significantly flattened or inverted in Band mice. (4) Except that RR interval at 2 week showed a slight decrease, there was no significant change in PR interval and RR interval in Band mice.

CONCLUSIONIn the present study, we found that the increase incidence of spontaneous arrhythmias, prologation of QT interval and changes in the amplitude of the J wave in the surface ECG during progression of mouse cardiac hypertrophy and failure, and the progressive pattern indicated that a gradual aggravation of the ventricular repolarization delay in this mouse model.

Animals ; Cardiomegaly ; physiopathology ; Disease Models, Animal ; Electrocardiography ; Male ; Mice

Child ; Hodgkin Disease ; therapy ; Humans

Background Sustained abnormal tear secretion in dry eye patients may lead to ocular surface and lacrimal glands in the state of long-term inflammatory cell infiltration.Lacrimal gland suffers immune attack by lymphoproliferative,and inflammation interferes normal gland secretion,in which chemokines and their receptors on lymphocytes play a key role.Objective This study was to investigate the inflammation mechanism of delayed allergy induced by Th1 cell in the development of dry eye.Methods Sixty eyes of 30 patients with dry eye and matched 30 healthy volunteers were enrolled in Affiliated First Hospital of Xinjiang Medical University from June to December in 2012.Schirmer Ⅰ test (S Ⅰ t),break up time (BUT) of tear and corneal fluorescence stain (FLS) were performed on the subjects,and conjunctiva epithelial cells were obtained using cytological method of conjunctiva imprinting.Positive cell rates of CC chemokine receptor 5 (CCR5) and CXC chemokine receptor 3 (CXCR3) were detected by flow cytology,and the relative expression levels of regulated upon activation of normal T cells exp ressed and secreted (RANTES),macrophage inflammatory protein (MIP)-lα and MIP-1β,monokine induced by interferon-γ (IFN-γ) (MIG),interferon-γ inducible protein 10 (IP10) and interferon-inducible T-cell α chemoattractant (I-TAC) mRNA were quantitatively assayed by real-time PCR.Differences of the positive rates of CCR5,CXCR3 and lignds were compared between dry eye group and normal control group.Relationship between the positive rates of CCR5,CXCR3 and BUT,S Ⅰ t,FLS scores was analyzed.Results The values of BUT,S Ⅰ t were (2.90±1.37) seconds and (4.00±2.49) mm/5 minutes in the dry eye group,which were significantly lower than (8.56±4.69) seconds and (11.31 ±5.23) mm/5 minutes in the normal control group (t =3.172,2.186,both at P＜0.05).FLS scores,positive rates of CCR5 and CXCR3 were0.90±0.57,(3.38±0.66) ％ and (2.64±0.47)％ in the dry eye group,showing significant elevations in comparison with 0.14±0.06,(2.12±0.21) ％ and (1.12±0.11) ％ in the normal control group (t=2.297,3.151,5.454,all at P＜0.05).In the dry eye group,the masculine rate of CCR5 was negatively correlated with BUT and S Ⅰ t (r=-0.473,-0.385,both at P＜0.05).The masculine rate of CXCR3 was negatively correlated with BUT and S Ⅰ t (r =-0.753,-0.684,both at P＜0.05).No considerable correlations between the positive rate of CCR5 with the positive rate of CXCR3 or FLS scores (r =0.231,0.336,both at P＜0.05).The relative expression levels of RANTES,MIP-1α,MIG and IP10 mRNA in the dry eye group were significantly higher than those in the normal control group (t =3.091,2.894,2.688,2.245,all at P＜0.05),but there were no significant differences in the relative expression levels of MIP-1β and I-TAC mRNA between the two groups (t =0.512,1.979,both at P＞0.05).The positive correlations were seen between the masculine rate of CCR5 with the relative expression of RANTES or MIP-1α mRNA (r=0.473,0.285,both at P＜0.05),but there was no obvious correlation between the masculine rate of CCR5 and the MIP-1 β mRNA expression(r=0.214,P＞0.05).In addition,the masculine rate of CXCR3 was positive correlated with the expressions of MIG and IP10 mRNA (r=0.553,0.314,both at P＜0.05),whereas the masculine rate of CXCR3 was not related to the expression of I-TAC mRNA (r=0.364,P＞0.05).Conclusions Dry eye is probably along with the long-term infiltration of inflammatory cells.The delayed allergy induced by Th1 cells and the nature killed cells is probably the primary cause to xerophthalmia.CCR5,CXCR3 and their ligands might be the regulative targets in the inflammation mechanism of dry eye.

Gaucher Disease ; Humans

Child ; Humans ; Lymphoma, Follicular ; diagnosis ; therapy

BACKGROUND: It is reported that the first line medical staff have experienced posttraumatic stress disorder(PTSD) symptoms after severe acute respiratory syndrome (SARS) events. However, there are few reports regarding the PTSD symptoms among SARS patients and the public in epidemic area. OBJECTIVE: To investigate the occurrence and main influence factors of PTSD among SARS patients and the public in epidemic area.DESIGN: Sampling investigation.SETTING: First Hospital of Shanxi Medical University. PARTICIPANTS: Totally 114 SARS patients and 93 people were selected from the epidemic area of Taiyuan city from August to September in 2003.METHODS: Self-made stressor-cognition questionnaire, edited impact of event scale-revised (IES-R), perceived social support scale (PSSS), simple cope scale questionnaire(SCSQ), self-esteem scale(SES) and eysenck personality questionaire(EPQ) were conducted to 114 SARS patients and 93 common people in epidemic area. Correlative analysis was performed as well.MAIN OUTCOME MEASURES: ① Detection rate of PTSD among SARS patients and the public in epidemic area. ② The score of intrusion factor,avoidance factor and hyperarousal factor of two populations. ③ The correlative analysis between IES-R score and influence factor among SARS patients and the public in epidemic area.RESULTS: The detection rate of suspect of PTSD among SARS patients was higher than that in public of epidemic area(55.1% , 31%, P = 0.000)The score of intrusion, avoidance and hyperarousal factor of SARS patients ( 11.37±9.54, 10.28±7.67, 6.55±6.20) was much higher than those of the public in epidemic area(5.64±4.68,5.58±4.74, 4.43 ±4.63,P = 0. 000) . The correlative analysis showed that the difference on PTSD incidence between SARS patients and the public of epidemic area might be due to the different stressor intensity and features. The negative response mode was the risk factor of both SARS patients and the public of epidemic area while self-esteem was the protective factor of SARS patients. CONCLUSION: ① PTSD symptoms had appeared among all the correlative populations although the exposure intensity of SARS event stress was different. The detection rate of PTSD and symptoms of intrusion, avoidance and hyperarousal were much common among SARS patients; ② The difference between groupswas due to the different stressor intensity as well as the different influence factors of different population.

Objective To compare the analgesic effects between methylene blue and adriamycin magnetic gelatin microballoons on blocking the cornu dorsale medullae spinalis of rabbit,and to investigate its mechanism.Methods 40 rabbits were randomly divided into 7 groups including sham operation group,gelatin microballoon controlled group(5mg and 15mg),methylene blue microballoon group(5mg and 15mg)and adriamycin microballoon group(5mg and 15mg).Under the guide of magnet,microballoons were infused into subarachnoid space of rabbits.The pain threshold of electronic stimulation and motor function of rabbits' hind limbs were observed continuously.The somatosensory evoked potential was recorded.Results The pain threshold was raised obviously(P

Adolescent ; Adult ; Aged ; Calcaneus ; injuries ; surgery ; Female ; Follow-Up Studies ; Fracture Fixation, Internal ; adverse effects ; Fractures, Bone ; physiopathology ; surgery ; Humans ; Male ; Middle Aged ; Postoperative Complications ; pathology ; physiopathology ; Recovery of Function ; Retrospective Studies ; Young Adult

Objective To study the changes in somatosensory evoked potential caused by injection of adriamycin magnetic gelatin microspheres into subarachnoid space of rabbits. Methods Thirty rabbits were randomly divided into 5 groups: sham operation group (group S), gelatin microspheres 5mg (group C1), 15mg of gelatin microsphere (group C2) controlled groups, and adriamycin microspheres 5mg (group A1) and 15mg (group A2) groups. Under the effect of magnet, microspheres were injected into the subarachnoid space of rabbits. The pain threshold of electronic stimulation, motor function of rabbits′ hinder limbs and somatosensory evoked potential (SEP) were observed continuously. Results Obvious rise of pain threshold was found in group A2 (P