The measurement of renaI volume by ultrasound was carried out in 488 women with sigleton pregnahey.Sixty-eight women had infants with the intrauterine Browth retardation(IUGR).The average of renal volume in nofmal pregnant females and females with IUGR increased 57.2% and 8.4% respectively during trimester.Hence,the neonatal weight outcomes were cortelated with the renal volume in their mothers.The specficity of measurement was 96%,and the sensitivity was 65%.The sensitivety was influenced by type of IUGR and individual difference of renal vohme.The positive predictive vaIue was 74%.

To study the effects of major components of Maijunan tablets, puerarin (Pue) and rhynchophylline (Rhy) on the transport of hydrochlorothiazide (Hct) Caco-2 cell monolayer model, the transport parameters of Hct, such as apparent permeability coefficient (P(app) (B --> A) and P(app) (A --> B)) and the ratio of P(app) (B --> A) versus P(app) (A --> B), were studied and compared when Hct was used solely and co-used with Pue and/or Rhy. The effects of drug concentrations, conveying times, P-glyprotein (P-gp) inhibitor verapamil and conveying Liq pH values on the transport of Hct in the above conditions were also investigated. The results indicated that the absorption of Hct in Caco-2 cell monolayer model could be a carrier-mediated active transport, along with the excretion action mediated by P-gp. Pue can decrease the excretion action of Hct mediated by P-gp, and Rhy had no significant effect on the transport of Hct. The co-use of Hct, Pue and Rhy enhanced the absorption of Hct. Meanwhile, conveying Liq pH value had significant influence on the transport of Hct. The absorption of Hct at pH 6.0 was higher than that at pH 7.4.

Objective To understand the characteristics of complications occurred after endoscopic thyroidectomy and its prevention and treatment.Methods Retrospective analysis on the clinical effects and complications of 105 cases of endoscopic thyroidectomy.ResultsThe average operative time was 85 minutes in the 105 cases.Five cases were transferred to open operation because of bleeding and 11 cases transferred for the intraoperative pathology of papillary carcinoma.After operation,subcutaneous ecchymosis occurred in 5 patients,subcutaneous emphysema in 10 cases,neck and anterior chest skin tautness and discomfort in 7 cases,parathyroid injury in 2 cases.No superior laryngeal nerve or recurrent laryngeal nerve injury was found in the 105 cases.ConclusionUnder strict control on operation indications,proficient and meticulous endoscope operative skills,familiarization with the anatomical structure of thyroid,and timely and proper treatment on postoperative complications can reduce and prevent the occurrence of complications.

Nucleolin is implicated in many pathological processes,particularly in inflammatory diseases,viral infection and neoplasms.Nucleolin levels are increased in several neoplasms and its localization changed,which play important roles in cell proliferation,apoptosis and metastasis of neoplasms.

Objective: To investigate the efficacy of acupuncture in cooperation with moxibustion for treating anxiety. Methods: A treatment group of 42 anxiety patients was treated by acupuncture in cooperation with moxibustion and a control group of 45 anxiety patients, with Western medicine. Results: The total efficacy rate was 92.9% in the treatment group and 71.1% in the control group. There was a significant difference between the two groups (P＜0.01). Conclusion: The effect was significantly better in the treatment group than in the control group. Acupuncture and moxibustion has no side effects and dependence.

Objective To investigate the expression pattern of STIL in gastric cancer and effect of silencing STIL on biological behaviors of SGC-7901 cell line.Methods The expression of STIL at mRNA and protein level were detected by qPCR and Western Blot,respectively.SiRNA against STIL was constructed and transfected into SGC-7901 cell line.The effect of Si-STIL on cell proliferation,cloning ability,cycle distribution and apoptosis was detected by MTT,colony-forming assay and flow cytometry,respectively.Results STIL was aberrantly expressed in gastric cancer compared with that in paratumor tissues.Downregulation of STIL level,inhibition of proliferation and cloning ability,increasing apoptosis and blocked cell cycle at S phase were observed after SGC-7901 cell line was treated with Si-STIL.Conclusion STIL was overexpressed in gastric cancer.STIL gene silencing effectively inhibited cell growth,transition of metaphase and promoted apoptosis of SGC-7901 cell line.

Objective To probe the CT findings of menisci in degenerative osteoarthrosis of knees joint.Methods CT features of meniscus damage in 151 cases with degenerative osteoarthrosis of knees joint collected randomly from 2003~2005 were retrospectively analysed.Results CT features included:abnormal outline of meniscus or its edge to be coarse in 16 cases(10.5%),local hypodense in the meniscus in 107 cases(70.8%),gap sign in 9 cases(5.9%) and vacuum sign in 19 cases(12.5%).Conclusion CT scan is of benefit for evaluating the meniscus damage in degenerative osteoarthrosis of knees joint,which can be used to plan the mode of treatment.

Objective To investigate the clonal origin of malignant cells in multifocal urothelial carcinoma. Methods p53 protein expressions were detected by immunohistochemistry in 22 pieces of tumor tissues from 10 patients with urothelial neoplasms.Mutation in p53 was inferred on the basis of immuno staining . Results The p53 protein expression of various tumors in the same patient completely matched, reflecting that the gene status of the multifocal tumor being the same. Conclusions The results strongly support the view that multifocal urothelial tumors are derived from the monoclonal cell.

Objective To discuss the complications of the bilateral uterine artery superselective embolization for uterine fibroids. Methods Fourty one cases(mean 42 years old) with uterine fibroids were approached by right femoral artery puncture; including thirty six cases of single hysteromyoma(33 cases with diameter 10 cm), and 5 cases of multi hysteromyoma. Bilateral uterine artery super selective 5F catheterization embolization were done along uterine descending artery(neck of uterus to vagina)with PVA particles. Rdsults Embolization succeeded with 100% and effective ratio was 92.3%. Sixteen of these cases showed post embolization syndrome, with something outflowed from vagina and 10 cases showed changes of emmenia but with no serious complications in all 41 cases. Conclusion Using bilateral uterine artery superselective embolization for uterine fibroids with PVP grains can decrease the incidence of complications. (J Intervent Radiol, 2006, 15: 280-282)

Objective:To construct pIRES-I-Ad?? bicistronic eukaryotic expression vector. Methods: Total RNA was acquired by TRIZOL method, the genes of I-Ad ?? chains were amplified through RT-PCR, respectively. The target genes were connected to pGEM-T vector and sequenced. Then the target genes were subcloned into pIRES bicistronic eukaiyotic expression vector and NIH3T3 cell line was transfected. Transfectant was screened by G418 antibiotics. Total RNA of transfectant was obtained by TRIZOL method, mRNA of foreign gene was examined by RT-PCR. Flow cytometry( FCM) was used to detennine foreign gene expression in protein level and surface expression in NIH3T3 cell line. Results: Bicistronic eukaryotic expression vector pIRES-I-AdaB was established. Foreign gene in mRNA level in transfectants was examined. Verified Ⅰ-Ad ?? chain wa3 expressed in high level expressed on transfected NIH3T3 cell line surface by FCM. Conclusion:Bicistronic eukaryotic expression vector pIRES-I-Ad ?? was constructed successfully. It is useful for studying antigen presentation and interaction between epitopes and MHC- Ⅱ molecule of BALB/c mouse.