Objective To evaluate the effect of interactive case teaching(ICT) method in teaching hematology.Methods Sixty students were randomly selected from 2009 grade clinical medicine majors in the Third Military Medical University and were divided into experimental group and control group,each group with 30 students.Students in experimental group used ICT； while those in control group used traditional teaching method.Closed book exam was conducted for students in experimental group and control group at the end of the semester； question types of the exam included concept questions (nouns explain,choice,short answer questions) for 70 points,cases analysis question for 30 points,a total of 100 points.Data were expressed as mean ± standard deviation ((x) ±s) and analyzed with SPSS 20.0 independent sample t test.Questionnaire was designed and feedback from students was statistically analyzed.Results There was no statistical differences between experimental group and control group in concept questions (P =0.580),however,in terms of cases analysis question,scores in experimental group were significantly higher than those in control group (P =0.000) ； total scores of experimental group were also significantly higher than those in control group (P =0.016).Feedback showed that the students highly valued the form and effect of ICT.Conclusions Using ICT in teaching hematology can arouse students' enthusiasm for study,cultivate their independent thinking and ability of problem-solving.Meanwhile,ICT can combined theoretical teaching with clinical practice well and improve the quality of teaching.

Objective To investigate the safety and superiority of fast track surgery (FTS)in elderly patients during the perioperative peri-od of laparoscopic cholecystectomy (LC).Methods The clinical data of 124 elderly patients who underwent LC at the Department of Gen-eral Surgery in our institution between January 2010 and March 2014 were assessed,with 62 cases assigned to FTS group and 62 cases to conventional method group.Anal exhaust time,feeding time,off-bed activity time,and length of hospital stay were compared between the two groups,and postoperative results and adverse reactions were recorded.Continuous data were analyzed using the independent-samples t test,and categorical data were compared using the chi-square test.Results Compared with the control method group,patients in the FTS group showed earlier postoperative anal exhaust,feeding,and off-bed activity,a shorter length of hospital stay,and a lower incidence of postoperative cardiovascular and cerebrovascular complications (P<0.05).Conclusion FTS is an effective approach to accelerate rehabil-itation in elderly patients after LC.

AIM: To compare the effects of histidine-tryptophan-ketoglurate(HTK)solution, University of Wisconsin(UW)solution and St.Thomas(ST) Cardioplegia solution Ⅱ on hypothermic preservation of isolated rat hearts. METHODS: Isolated hearts from male Wistar rats were mounted on a Longendorff apparatus to estimate left ventricular end-diastolic pressure(LVEDP),left ventricular developed pressure(LVDP), rate of change of left ventricular pressure(d p /d t max ,d p /d t min ) and coronary flow(CF).Twenty-four rats were randomly divided into three groups: HTK group, UW groups and ST group.They were arrest and stored at 4℃ in each preservation solution for 6 hours. Following storage, they were reperfused and postpreservative function was measured to assess cardiac functional recovery. Frozen samples were analyzed for concentration of adenosine triphosphate. RESULTS: The concentration of adenosine triphosphate in three groups was significantly different( P

Objective To investigate the effect of atorvastatin on the curative effect and cardiovascular events in patients with unstable angina pectoris.Methods128 patients with UAP were enrolled in Jinhua Central Hospital from August 2014 to August 2016, and 128 patients with UAP were randomly divided into observation group and control group.All the patients were given low salt and low fat diet.Two groups of patients were anti-platelet aggregation, blood pressure, lipid-lowering and other conventional treatment, observation group on the basis of simultaneous atorvastatin calcium 40mg/d, oral, once every month.The levels of TC, TG and LDL-C, HDL-C and the incidence of cardiovascular events were compared between the two groups before and after treatment.ResultsThere was no case of cardiac death in the observation group and the control group.The incidence of cardiovascular events in the observation group was 9.38%, which was significantly different from that in the control group (P<0.05).The levels of TC, TC and LDL-C in the observation group were significantly higher than those before treatment and control group, respectively.The clinical effect of the observation group was significantly better than that of the control group, the difference was significant (P<0.05).ConclusionAtorvastatin can significantly improve the efficacy of unstable angina treatment, and reduce blood lipid levels, reducing the incidence of cardiovascular events, improve the prognosis.

Objective To investigate the relationship between the expression of Fas or FasL and the apoptosis of peripheral blood T and B lymphocytes in systemic lupus erythematosus (SLE) patients. Methods The expression rates of Fas and FasL and apoptosis rates of peripheral blood T and B lymphocytes were detected by flow cytometry (FCM) in 30 active SLE patients and 30 inactive SLE patients and 30 healthy controls. Results There were significant difference in the expression rates of Fas of T and B lymphocytes among three groups (P0.05). In addition, the activity of SLE had no correlation with the expression rates of Fas or FasL on T or B lymphocytes but has positive correlation with the apoptosis rates of T or B lymphocytes. And no correlation was found between the expression rate of Fas or FasL and the apoptosis rate of neither T nor B lymphocytes. Conclusion The apoptosis of peripheral blood lymphocytes is influenced by many factors in SLE patients, but is abnormally increased at last, especially in active SLE. It is inferred that the increase of apoptosis rate of peripheral blood lymphocytes plays an important role in the pathogenesis of SLE.

Acupuncture Therapy ; Diarrhea ; therapy ; Female ; Humans ; Young Adult

Objective To compare the clinical features of space infections in the oral maxillofacial region between patients with diabetes mellitus and patients without diabetes.Methods A retrospective study was conducted on 61 patients diagnosed with space infections in the oral maxillofacial region and receiving treatment at the department of oral and The 4nd Affiliated Hospital of Kunming Medical University between 2013 and 2016.The patients were divided into 2 groups:diabetic group (n =28) and non-diabetic group (n =33).The study variables were demography,etiology,clinical and laboratory examinations (bacteriology,admission blood glucose level,admission white blood cell count and percent of neutrophil on admission).The outcome was compared.The length of hospital stay and complications were also compared.Statistical analyses of the results between groups were performed using the t test and chi square test.Results For oral and maxillofacial space infection,there was no statistically significant difference in gender between the diabetic group and (28 cases) and non diabetes group (33 cases) (x 2 test,P=0.5929);while there were statistically significant differences between two groups in age (t test,P＜0.001) and in the number of infection space (t test,P＜0.001);there was no significant difference between the two groups in the sources of infection (x 2,P＞0.05);The main source of infection was odontogenic,followed by adenogenous.There were statistically significant differences between two groups in blood glucose concentration at admission (t test,P＜0.001) and in the hospitalization time and complications (t test and x 2 test P＜0.05),while no statistically significant differences in the white blood cells count and neutrophil count (t test,P＞0.05).Compared with the non diabetic group,for patients in the diabetic group,the age of onset was older,the involvd gaps were more,the blood glucose concentration was higher on admission,the length of stay was longer,complications were more likely to occur,and the consequences were more serious.Conclusion Compared with non diabetes group,space infection in oral and maxillofacial region is more serious and the hospital stay is longer and has more complications in diabetic patients which should be paid higher attention to.

Background Sustained abnormal tear secretion in dry eye patients may lead to ocular surface and lacrimal glands in the state of long-term inflammatory cell infiltration.Lacrimal gland suffers immune attack by lymphoproliferative,and inflammation interferes normal gland secretion,in which chemokines and their receptors on lymphocytes play a key role.Objective This study was to investigate the inflammation mechanism of delayed allergy induced by Th1 cell in the development of dry eye.Methods Sixty eyes of 30 patients with dry eye and matched 30 healthy volunteers were enrolled in Affiliated First Hospital of Xinjiang Medical University from June to December in 2012.Schirmer Ⅰ test (S Ⅰ t),break up time (BUT) of tear and corneal fluorescence stain (FLS) were performed on the subjects,and conjunctiva epithelial cells were obtained using cytological method of conjunctiva imprinting.Positive cell rates of CC chemokine receptor 5 (CCR5) and CXC chemokine receptor 3 (CXCR3) were detected by flow cytology,and the relative expression levels of regulated upon activation of normal T cells exp ressed and secreted (RANTES),macrophage inflammatory protein (MIP)-lα and MIP-1β,monokine induced by interferon-γ (IFN-γ) (MIG),interferon-γ inducible protein 10 (IP10) and interferon-inducible T-cell α chemoattractant (I-TAC) mRNA were quantitatively assayed by real-time PCR.Differences of the positive rates of CCR5,CXCR3 and lignds were compared between dry eye group and normal control group.Relationship between the positive rates of CCR5,CXCR3 and BUT,S Ⅰ t,FLS scores was analyzed.Results The values of BUT,S Ⅰ t were (2.90±1.37) seconds and (4.00±2.49) mm/5 minutes in the dry eye group,which were significantly lower than (8.56±4.69) seconds and (11.31 ±5.23) mm/5 minutes in the normal control group (t =3.172,2.186,both at P＜0.05).FLS scores,positive rates of CCR5 and CXCR3 were0.90±0.57,(3.38±0.66) ％ and (2.64±0.47)％ in the dry eye group,showing significant elevations in comparison with 0.14±0.06,(2.12±0.21) ％ and (1.12±0.11) ％ in the normal control group (t=2.297,3.151,5.454,all at P＜0.05).In the dry eye group,the masculine rate of CCR5 was negatively correlated with BUT and S Ⅰ t (r=-0.473,-0.385,both at P＜0.05).The masculine rate of CXCR3 was negatively correlated with BUT and S Ⅰ t (r =-0.753,-0.684,both at P＜0.05).No considerable correlations between the positive rate of CCR5 with the positive rate of CXCR3 or FLS scores (r =0.231,0.336,both at P＜0.05).The relative expression levels of RANTES,MIP-1α,MIG and IP10 mRNA in the dry eye group were significantly higher than those in the normal control group (t =3.091,2.894,2.688,2.245,all at P＜0.05),but there were no significant differences in the relative expression levels of MIP-1β and I-TAC mRNA between the two groups (t =0.512,1.979,both at P＞0.05).The positive correlations were seen between the masculine rate of CCR5 with the relative expression of RANTES or MIP-1α mRNA (r=0.473,0.285,both at P＜0.05),but there was no obvious correlation between the masculine rate of CCR5 and the MIP-1 β mRNA expression(r=0.214,P＞0.05).In addition,the masculine rate of CXCR3 was positive correlated with the expressions of MIG and IP10 mRNA (r=0.553,0.314,both at P＜0.05),whereas the masculine rate of CXCR3 was not related to the expression of I-TAC mRNA (r=0.364,P＞0.05).Conclusions Dry eye is probably along with the long-term infiltration of inflammatory cells.The delayed allergy induced by Th1 cells and the nature killed cells is probably the primary cause to xerophthalmia.CCR5,CXCR3 and their ligands might be the regulative targets in the inflammation mechanism of dry eye.

Objective: To intvestigate the effects of processing on contents of matrine and oxymatrine of Radix Sophorae Flavescentis. Methods: The determinations of matrine and oxymatrine were carried out by dual wavelength TLC scanning. Results: There were obvious differences between the crude and the different processd products of Radix Sophorae Flavescentis in matrine and oxymatrineConclusion: The processing temperature, material, wine and vinegar can make the contents of matrine and oxymatrine of Radix Sophorae Flarescentis change.

Objective To investigate the effect of miR-141 up-regulation through miR-141 mimic transfection on proliferation,apoptosis and cell cycle of hepatocellular carcinoma (HCC) cells.Methods Real-time polymerase chain reaction (qPCR) was employed to detect differential expression of miR-141 in normal hepatic epithelial cells (L02) and hepatocellular carcinoma cells (HepG2).Furthermore,miR-141 mimics were used to transfect into HepG2 to up-regulate the expression of niR-141 (experimental group).Blank group (CON) and negative control group (miR-NC) were used as control group.qPCR was used to detect expression of miR-141.CCK8 assay was used to detect the proliferation of HepG2 cells.Flow cytometry was used to detect the apoptosis and cell cycle of HepG2 cells.Results The results of qPCR showed that miR-141 was significantly down-regulated in HepG2 cells (0.64 ± 0.13) compared to L02 cells (1.00 ± 0.18),and the difference was significant (P ＜ 0.05).Relative expression of miR-141 was significantly increased after transfection (3.33 ± 0.66),compared to CON group (1.00 ± 0.17,P ＜ 0.05) and miR-NC group (1.08 ±0.14,P ＜0.05).CCK8 assay showed that the absorbance values of HepG2 of miR141 group at 48,72,96 h (0.67 ± 0.07,1.17 ± 0.05,1.36 ± 0.03) were all decreased significantly compared with those in CON group (0.81 ±0.02;1.42±0.03;1.73 ±0.05,all P＜0.05) and miR-NC group (0.78 ±0.01;1.38 ±0.02;1.69 ±0.01,all P ＜0.05).The results showed that miR-141 group [(11.81 ± 0.23)％] had significantly increased apoptosis rate compared to CON group [(4.18 ± 0.18) ％] and miR-NC group [(4.04 ± 0.08) ％],and the difference was statistically significant (P ＜ 0.05).miR-141 group had decreased percentage of S phase cells [(19.89 ± 2.78) ％] compared to CON group [(31.87 ± 1.00) ％,P ＜ 0.05] and miR-NC group [(30.49 ± 1.73) ％,P ＜ 0.05],while miR-141 group had significantly increased percentage of G1 phase cells [(74.74 ±2.03)％] compared to CON group (60.85 ± 1.69) ％ and miR-NC group (60.93 ± 1.95) ％,and the differences were all statistically significant (all P ＜ 0.05).Conclusions miR-141 was in low expression in HepG2 cells.Upregulation of miR-141 could specifically inhibit proliferation and promote apoptosis of HepG2 and change the distribution of cell cycle.