Bacterial disease is still the important disease hazardous to health of human being and animals.The bacterial mutation technique is an important bacterial research technique,including traditional physical and chemical and biological method,and modern genetic mutation technique.The bacterial genetic mutation technique is one of the research focus in bacteriology nowadays,the mutation strategy varies with different kinds of genetic:mutation technique.The development and application of bacterial mutation technique offers new tools for research on new bacterial vaccine,bacterial genetic function and genetic therapy etc.

An innovative educational model characterized as interesting-guiding is created in the acupuncture educational practice for foreign students. According to the innovative educational theories, teaching orders are rearranged, new teaching methods are used, and passive indoctrination is changed into creative studies and discussion, thus the educational quality is improved.

The concept and characteristics of omnimedia were outlined, the effects of omnimedia on traditional in-formation service model, storage types of information resources, and personal service in medical library were analyzed, and a number of new information service models in the omnimedia era were put forward.

Objective To compare the early efficacy of two different ways of rehabilitation exercises in total knee arthroplasty (TKA) patients. Method From January 2011 to January 2014,136 patients having undergone TKA were randomly divided into two groups. In group A , patients exercised under the guidance with doctor. In group B, patients exercised by using continuous passive movement (CPM) machine. Compared the data from the two groups including range of motion (ROM) of the knee joint, knee function score (KFS) and circumference of the knee joint, t test of independent sample was utilized in the research. Results All patientswere followed up in 4 weeks. The ROM and KFS of Group A was significantly better than Group B (P < 0.05). The knee circumference had no statistically significant difference between two groups (P> 0.05).Conclusion Group A and B are effective methods in TKA patients in early rehabilitation training. But training under the guidance of doctor has greater advantages than CPM.

As the efficacies of chemotherapy in the treatment of advanced non-small cell lung cancer(NSCLC) have reached a plateau, the molecularly targeted therapy becomes a new method to improve curative effects.The molecular targeted drugs include epidermal growth factor receptor inhibitors, anti-angiogenesis drugs, etc.As one of the representative drugs of EGFR inhibitors, Gefitinib, has a low clinical benefit index.Erlotinib, the first EGFRTK inhibitor, can enhance the survival of patients, and may serve as a potential choice for the first-line treatment of advanced NSCLC.Bevacizumab combined with chemotherapy has shown a favorable clinical effect in the first-line treatment, and it is also the first anti-angiogenesis drug approved by the FDA that can be used in cancer treatment.These drugs, together with clinical and experimental progress in many other targeted drugs, have shown a favorable prospect for targeted drugs in the treatment of advanced non-small cell lung cancer.

The formation of carcinomas of liver, lung and kidney in rats by feeding aminopyrine (AP) plus sodium nitrite (Na NO2) in diet was investigated. 72 young Wister rats were divided into A,B,C,D group. Group A was set as control receiving basal diet and tap water. Another 3 groups were given basal diet containing AP plus Na NO2 (2g/kg each). However, group B was given tap water, group C ascorbic acid solution, group D sea buckthorn (Hippophae rhamnoides L.) juice for drinking. After 38 week's feeding, group B developed tumors in the livers (17/17), lungs (6/17) and kidneys (4/17) and the average life-span was 195 days, group D delayed the development of tumors and the average life-span was 270 days, significantly longer than that of group B (195 days, p

Hepatic cancer is currently the fifth most common malignant neoplasm in the world.Surgical resection is considered as radical treatment.Patients with hepatic cancer in middle or advanced stage according to the Barcelona clinic liver cancer staging system (BCLC) are usually with huge and (or) multinodular lesions and vascular invasion,which are not generally recommended for surgical resection because of high operative mortality,recurrence rate and dismal survival benefit.However,many centers have proved an opposite and encouraging result against the opinions above.With the development of surgical techniques and intensive medical care,the concern of high postoperative mortality for middle or advanced stage hepatic cancer patients is no longer unsolvable.Precise preoperative assessment is essential.The estimation of the liver functional reserve has developed from simple Child-Pugh score to an integrated system including computed tomography evaluation,indocyanine green clearance test,hepatic venous pressure gradient,etc.The estimation of the remnant liver volume after hepatectomy is especially important for surgical treatment for the middle or advanced stage hepatic cancer.Insufficient liver remnant was absolute contraindication for major hepatectomy because of high incidence of postoperative liver failure.In-situ liver transection with one branch of the portal vein ligation has been invented as a novel method to stimulate fast liver regeneration; by this way,a second-stepped radical resection can be performed with a plenty of liver remnant one week later.However,the reliability for hepatic cancer patients with liver cirrhosis is still unknown.Meticulous surgical procedure is another key factor for a safe major hepatectomy.Radical resection is most expected to provide better survival.The development of the technique of liver blood flow occlusion has a markedly influence on partial hepatectomy.Highly selective occlusion and even occlusion-free hepatectomy can reduce warm ischemia injury and improve postoperative survival.Anterior approach is a reasonable maneuver for huge hepatic cancer resection.Cancer thrombosis usually indicates poor prognosis,however,if en-bloc resection or separate thrombectomy can be achieved,surgical resection for the middle and advanced stage hepatic cancer still can provide a better survival benefit to this category of patient than palliative treatment as TACE.

The incidence of cervical neoplasms complicated with pregnancy is rising,with delaying of reproouctive age.Because of the prevalence of cytology in pregnancy.eervical neoplasnis complicated with pregnancy are mostly detected early. All patients with cytological abnormalities should undergo colposcopy, and when necessary,they should undergo cervical biopsy.Conization is reserved for patients with suspected invasion.In cases of invasive carcinoma detected up to the 12th week of pregnancy,immediate treatment is prioritized.Regarding diagnoses made during the second trimester,in early-stage invasive cancers,delay of therapy seems to be safe.fetal pulmonary maturity can be awaited.And the use of neoadjuvant chemotherapy to stabilize the disease until the time of delivery appears to be viable.Doctors should draw up personalized program for the patients with cervical neoplasms complicated with pregnancy according to stage of disease, gestational weeks and the patient's desire for continued pregnancy.Cervical neoplasms complicated with pregnancy are mostly early-stageinvasive cancers, so prognosis is good.

Epithelial-mesenchymal transition (EMT) not only can endow cells migration and invasion characteristics,but also can make tumor cells obtain self-renewal ability and have the characteristics of stem cells,which might result in cancer stem cell (CSCs).There are the same molecular mechanism and microenvironment between EMT and CSCs,which have great clinic significances for the diagnosis and treatment of the aggressive cancers.Moreover,many studies show that miR-200 could regulate EMT and CSC,participate in the tumor invasion and metastasi,and promote the research of targeted cancer therapy.

Objective To explore the clinical effect of uterine artery embolization on cesarean scar pregnancy (CSP) and curettage opportunity.Methods Sixty-three patients with CSP received curettage after uterine artery embolization with gelfoam were selected.Among of them,21 cases received curettage within 24 h after uterine artery embolization (group A); 20 cases within 1-3 d (group B);22 cases within 4-7 d (group C).The intraoperative haemorrhage,serum β-human chorionic gonadotropin (β-hCG)level resolution time,duration of hospital stay,hospitalization cost,postoperative complications were observed after the termination of the pregnancy.Results The duration of hospital stay and hospitalization cost in group A and group B were (10.5 ± 3.9),(10.2 ± 5.2) d and (7353 ± 962),(7594 ± 1032) yuan,respectively,and there was no significant difference (P ＞ 0.05).Which was lower than that in group C [(13.9 ± 5.4) d,(10 980 ± 1534) yuan],and there was significant difference(P ＜ 0.01).The intraoperative haemorrhage,serum β-hCG level resolution time and postoperative complications in group B and group C were(20.2 ± 8.8),(20.7 ± 9.3) ml and (25.9 ± 9.3),(25.7 ± 8.1) d and 40.0％(8/20),40.9％(9/22),and there was no significant difference(P＞ 0.05).But which was better than those in group A[(35.0 ± 10.7) ml,(20.5 ± 7.7) d,66.7％ (14/21)],and there was significant difference (P ＜0.01).Conclusions The treatment of curettage after the uterine artery embolization is a safe and effective method for CSP.There are fewer complications,shorter hospital stays and fewer hospitalization costs if receiving curettage within 1-3 d after uterine artery embolization.