In this report,five mouse hybridoma cell lines secreting monoclonal antibodies to recombi-nant human interferon—?2a(rHu IFN—?2a)were raised.All the monoclonal antibodies with IgGl subclass react with rHu IFN—?2a detected by indirect ELISA with the titers ranging from 1:320,000 to 1:10,240,000 but have no cross reactivity with rHu IFN?l,? and proteins from recombinant E.coli.Two of monoclonal antibodies,designated 3F1 and 5G10,have the ability to neutralize antiviral activity of rHu IFN—?2a.By a competitive ELISA the five monoclonal anti-bodies can be divided into three categories based on their epitope specificities.A sandwich ELISA established by employing 3F1 and 5G10 monoclonal antibodies could detect rHuIFN-?2a or rHuIFN-?2b as few as 60pg/ml?Moreover,3F1 monoclonal antibody displayed a satisfactoryproperty in affinity chromatography for rHuIFN?2a,which is able to match a monoclonal anti-body NK2 from CellTech Corporation.

Objective To study on the correlation of hepatic fibrosis with angiotensin Ⅱ (Ang Ⅱ ) in the hepatitis B patients . Methods The electrochemical luminescence and the rate method were adopted to detect hyaluronidase (HA) and Ang Ⅱ levels in 92 cases of acute hepatitis B (acute group) ,108 cases of chronic hepatitis B(chronic group) and 50 healthy people(control group) , and the detected results were performed the correlation analysis .Results Compared with the acute group and the control group ,the HA and Ang Ⅱ levels in the chronic group were increased significantly (P<0 .05) .The HA and Ang Ⅱ levels had statistical differ-ence between the acute group and the control group (P<0 .05) .Further analysis showed that there was a positive correlation be-tween HA and Ang Ⅱ levels(r=0 .629 ,P<0 .05) .Conclusion There the HA level is closely related with the Ang Ⅱ level ,and both of them participate in the process of liver fibrosis ,their levels have the guiding significance in evaluation of severity of liver fi-brosis in the patients with hepatitis B .

Objective To explore the application of ultrasonography (US) 5-grade system in the diagnosis of nasopharyngeal carcinoma(NPC).Methods A total of 150 patients suspected of having NPC underwent ukrasonography and MR imaging.All the lesions were scored according to the two different methods.Pathological results were made the golden criteria.The diagnosis accuracy of ultrasonography and MR imaging for NPC was evaluated using receiver-operating curve (ROC) analysis by Medcalc software.Results Both ultrasonography and MR imaging exhibited a good diagnostic performance for NPC with area under the curve (AUC) values of 0.958 and 0.987,respectively.The AUC difference of the two methods were statistically significant(Z =2.827,P =0.005).Conclusions Ultrasonography 5-grade system was an initial quantitative criterion for nasopharyngeal carcinoma diagnosis.The diagnosis accuracy was high although lower than MRI.But it could provide an overall economical and convenient complementary technique for clinical diagnosis.

?AIM: To observe the efficacy of 25G vitrectomy for traumatic cataract.?METHODS:Retrospective analysis of 70 cases (70 eyes) of traumatic cataract treated by 25G vitrectomy with lensectomy from February 2013 to February 2015 in our hospital was made for the study.In the patients, there were 43 males ( 43 eyes ) , 27 females ( 27 eyes ) , aged from 22-51 years old with an average of 35.23±2.54 years, mean visual acuity was 0.10±0.03.They were all followed up for 6 -12mo postoperatively. Anterior segment, fundus, complications, best corrected visual acuity ( BCVA ) and intraocular pressure were observed during the follow - up. BCVA preoperatively, 1wk postoperatively, 1 mo postoperatively, 6mo postoperatively were compared.? RESULTS: All the patients underwent the surgery successfully, and visual acuity improved at different degrees. The differences of BCVA preoperatively compared with 1 and 6m postoperatively were all statistically significant ( P <0. 05 ). Postoperative complications:5 cases with corneal edema, 10 cases with ocular hypertension, 4 cases with ocular hypotension, 5 cases with retinal spotting.The symptoms improved after symptomatic and supportive treatment.? CONCLUSION: 25G vitrectomy with lensectomy is effective on treating traumatic cataract, with decreased operation risk, complications and improved visual acuity.

As a secreted glycoprotein,Dickkopf-1 (DKK1) is closely related to tumor metastasis,and it is highly or lowly expressed in tumor.The high expression of DKK1 promotes the metastasis of a variety of malignant tumors including liver cancer,gastric cancer,non-small cell lung cancer and breast cancer.Recent studies have found that DKK1 is inextricably bound up with the latent metastasis of tumor.

Objective To evaluate the timing,feasibility and necessity of early laparoscopic cholecystectomy (LC) in management of patients with mild to moderate acute pancreatitis with gallbladder stones.Methods The clinical data of 75 patients with mild to moderate acute pancreatitis and gallbladder stones treated from September 2010 to August 2014 in our hospital were analyzed retrospectively.32 patients underwent LC within 48 hours of the pancreatic attack.The results were compared with those from 43 patients with delayed LC.Results All the patients were operated by experienced surgeons.There were no significant differences between the two groups in operation time,postoperative complications,intraoperative blood loss,conversion rates,white blood cell count,percentage of neutrophils and blood amylase before and after the operation (P ＞0.05).Patients who received delayed LC had longer hospital stay [(10.6 ± 1.3) vs (17.1 ± 1.8),P ＜ 0.05].The readmission rate in patients with delayed LC was 30.2％ (13/43),and most admissions occurred within 2 ～4 weeks of hospital discharge.In patients with early LC,one patient developed necrotic pancreatitis and died of septic shock.All the remaining patients were cured.Conclusions It is safe,feasible and necessary to perform LC within 48 h in patients with mild to moderate acute pancreatitis and gallbladder stones.Such patients have a high readmission rate and the best timing of delayed LC is within 2 ～ 4 weeks after discharge from hospital.

OBJECTIVE:To investigate the characteristics and regularity of ADR in our hospital,and to provide reference for clinical rational drug use. METHODS:262 ADR reports of the hospital during 2013-2014 were summarized and analyzed. RE-SULTS:Among 262 cases of ADR,most of ADR occurred in elderly patients aged above 60 years old,accounting for 46.56%. 239 cases were induced by intravenous drip,accounting for 91.22%;anti-infective drugs induced 97 cases of ADR,accounting for 37.02%,among them there were 48 cases of cephalosporin-induced ADR,accounting for 49.48%(total 97 cases);most of them were lesion of skin and its appendants,being 96 cases and accounting for 36.64%. Among 262 cases,132 cases were improved af-ter drug withdrawal(50.38%);no drug sequelae or death cases was found. CONCLUSIONS:To master the characteritics and reg-ularity of ADR contribnte to redcue and aroid the repeat of ADR.

Objective: To study bacterial and lactobacilli communities in stomach of neonatal piglets from 7 to 35(two weeks after weaning) days of age.Methods: The semi-quantitative Dilution-PCR,PCR-DGGE and sequencing were used to analyze the shifts of stomach total bacterial and lactobacilli community.Results: Stomach pH value was around 3.0 before weaning and increased apparently after weaning.Dilution-PCR showed that bacterial concentration in stomach of piglet was very low before weaning and increased significantly after weaning.The DGGE profiles from total bacteria in stomach showed that the bands of samples before weaning were very simple and the predominant band belonged to high GC bacterium.After weaning,some new predominant bands appeared and the previous high GC bacteria disappeared simultaneously.Sequence analysis indicated that these new bands were Escherichia fergusonii,Escherichia coli,Streptococcus gordonii and Streptococcus suis,all of which were potential pathogens for piglets.The similarity of DGGE profiles from stomach lactobacilli showed weaning stress had no obvious effect on lactobacilli community.Conclusion: Weaning stress could cause stomach pH increase apparently and induce potential pathogens proliferation in stomach of piglets.

Atherosclerosis ; Dyslipidemias ; Humans

Objective To investigate the effects of different ventilations during the induction of anesthesia on postoperative nausea and vomiting in patients undergoning laparoscopic cholecystectomy. Methods One hundred and twenty patients undergoing laparoscopic cholecystectomy were randomly divided into M group and V group according to random number table, 60 patients each group. The patients in M group performed manual ventilation and the patients in V group performed mechanical ventilation. According to different tidal volume, V group were randomly divided into three groups (20 patients each group:V1,V2 and V3 group, respectively recieved body weight × 4, 6 and 8 ml/kg. After tracheal intubation,the operations were completed by maintaining the end of CO2 partial pressure (PETCO2)from 35 to 45 mmHg, 1 mmHg = 0.133 kPa. Tidal volume, peak airway pressure (Ppeak) and PETCO2 in anesthesia induction,and intraoperative gastric insufflation and postoperative nausea and vomiting were recorded and compared. Results The incidence rate of gastric insufflation, postoperative nausea and vomiting in V group were significantly higher than those in M group:50.0%(30/60) vs. 21.7%(13/60), 45.0%(27/60) vs. 18.3%(11/60), 26.7%(16/60) vs. 10.0%(6/60), P<0.05. The level of Ppeak in M group, V2 group and V3 group were signicantly higher than that in V1 group: (13.55 ± 1.95), (12.05 ± 1.23), (18.35 ± 3.71) cmH2O vs. (13.55 ± 1.95) cmH2O, 1 cmH2O = 0.098 kPa, P<0.05. The level of PETCO2 in M group, V2 group and V3 group were signicantly lower than that in V1 group:(19.60 ± 4.31), (27.75 ± 1.94), (23.60 ± 4.45) mmHg vs. (29.90 ± 2.40) mmHg, P<0.05. Conclusions Manual ventilation during the induction of anesthesia in patients undergoning laparoscopic cholecystectomy is better than mechanical ventilation . Proper manual positive pressure ventilation and volume mechanical ventilation with low tidal (4 ml/kg) may allow for decreasing intraoperative gastric insufflation and postoperative nausea and vomiting.