Objective:To study the effect of dressing with insulin on the expression of HIF-1?and VEGF in deepⅡ degree burn wound healing of diabetic rats and explore the possible mechanisms. Methods:42 rats,either male or female,were established models of deepⅡ burn wounds with diabetes,which was induced by streptozotocin (STZ). They were completely randomly divided into two groups,that is the insulin group and the control group. Dressing changes with insulin and saline dressings twice daily,respectively. Full-thickness tissues of the wounds were collected respectively in the 1st,2nd and 3rd weeks after the treatment. The expression of hypoxia inducible factor-1?mRNA (HIF-1?mRNA)was detected with RT-PCR;and the positive staining of HIF-1?and VEGF and microvessel density in the wound healing were determined with IHC. Results:Compared with the control,the healing rate was obviously increased, the expression of HIF-1?mRNA and the positive expression of HIF-1?were significantly decreased (P

In this paper we report the results of 1434 differert minority college students in Yunnan in response to SCL-90 test. Results showed that the mean of every factor was from 1.56 to 2.12. The factor mean was different between male and female except the hostility, psychotism, PSDI; and factor mean of female was higher than that of male. There was no difference be- tween minority students and Han students. The factor mean of the obsession, compulsion and interpersonal sensitivity was higher in different minority students.

Dr. Rachel Yudkowsky et. al. have published the paper in Academic Medicine [2008,83 (10 Suppl):S13–S16] demonstrating a reformed Angoff method in which items were assigned to "Yes/No/Maybe" three-level categories for judges to estimate whether a borderline students would or would not accomplish each item,compared with "Yes/No" two-level categories. Five judges were provided three-level Angoff ratings for seven checklists used in a clini-cal skills exam for fourth-year students. 45% of 121 items had at least one "Maybe" rating. About 10% of all ratings were in the "Maybe" category. Case failure rates varied considerably depending on the simulated severity of ratings. Overall failure rates were not substantially impacted. The three-level Angoff retains the cognitive simplicity of the Yes/No Angoff while addressing the challenge of items midrange for the borderline candidate and avoids the potential bias.

Objective To investigate the causes of intra-abdominal hernia and improve the level of early (diagnosis) and therapy of intra-abdominal hernia. Methods A retrospective analysis of the clinical data of 68 cases of intra-abdominal hernia was made. Results The postoperative confirmed diagnosis was para-occal hernia in 1 patient;herniation of efferent jejunal loop through the space between the afferent jejunal loop and the mesocolon after Billroth II gastrectomy in 6 patients,one of which had bowel necrosis,all of the 6 patients were cured after reduction of the hernia or enterectomy after and enteroanastomsis;internal henia though (ruptured) pelvic (peritoneum) after Miles operation in 4 patients;internal hernia though the space between the (descending) colon and the lateral abdominal wall after colostomy of descending colon in 6 patients;internal (hernia) caused by (adhesion) of omentum to the intestinal wall of sigmoidostomy in 1 patient;internal hernia through hiatuses caused by postoperative or post-peritonitis adhesions to the peritoneum,ovaries,urinary (bladder),uterus,(intestinal) wall or between loops of intestine in 50 patients(73.5%),including 3 cases of bowel (necrosis).All of those patients were cured after reduction of the hernias,repair of the hiatuses,release of (adhesions) or enterectomy and enteroanastomosis. Conclusions Preoperative diagnosis of internal abdominal hernia is difficult.The possibility of internal abdominal hernia should be cansidered in patients with a history of operation,and who complain of frequent abdominal pain or bowel obstruction.Early diagnosis and prompt (operation) is necessary to prevent the occurrence of bowel strangulation and bowel necrosis.

Percutaneous transluminal coronary angioplasty (PTCA) is a very effective approach to treat coronary artery disease.However,restenosis after PTCA affects 40% to 60% of patients in the months after an initially successful intervention.Although a number of new techniques and pharmacological approaches have been tried to reduce the rate of restenosis,only a few have shown even preliminary efficacy.Radiation therapy seems to provide an interesting,nonpharmacological approach to prevent the restenosis after PTCA during recent years.The experiments and clinical data of this new approach are reviewed in this paper.

Objective To explore the role of new emergency mode "Green track" in treatment on traffic injuries. Methods The data of 2 947 patients with traffic injuries rescued by prehospital emergency "green track" and inhospital emergency treatment from March 1998 to January 2009 were retrospectively studied in aspects of prehospital and inhospital treatment methods and their results. Results Of all patients, 2 089 patients were treated surgically and 858 patients treated conservatively, in which 2 736 patients (92.8%) were cured but 211 (7.2%) died, with mortality rate of 7.2%. Head, face and limbs were the main injury parts. Of all, 1 874 patients (63.6% ) were admitted into the hospital within one hour after injury. Conclusions The new emergency mode "Green track" , either prehospital or inhospital, can markedly improve success rate of treatment for traffic injuries, increase survival rate and is of importance to be clinically applied.

Objective To explore the role of JNK signaling pathway in epithelial mesenchymal transition (EMT)process of human alveolar epithelial cells A549 induced by TGF‐β1 in vitro.Methods Human alveolar epithelial cells (A549)cultured in vitro were divided randomly into three groups :normal group ,TGF‐β1 group ( treated by TGF‐β1 with 10 ng/mL)and inhibitor group (treated by 10 ng/mL TGF‐β1 and 20 μmol/L Sp600125).Morphological observation on the cells was performed under light microscope after culturing in 3% serum medium for 72 h. The expression of E‐cadherin (E‐cad ,a epithelial cell marker) ,α‐smooth muscle actin (α‐SMA ,a mesenchymal cell marker)and Collagen fibers Ⅰ(ColⅠ ,a mesenchymal cell marker)were tested by RT‐PCR.The level of JNK phosphorylation (p‐JNK)was detected through Western blot.All experiments were repeated three times at least.Results The normal human alveolar epithelial cells (A549)cultured invitro were arranged closely like peb‐bles.E‐cad expressed at a certain level ,while the expression ofα‐SMA ,ColⅠand p‐JNK was weakly detected.In TGF‐β1 group , the cells were spindle‐shaped ,the expression of E‐cad was reduced ,while the expression ofα‐SMA ,ColⅠand p‐JNK was signifi‐cantly increased 72 h after treatment of TGF‐β1. However ,compared with TGF‐β1 group ,spindle‐shaped cells in the inhibitor group were recovered after 72 h being treated by TGF‐β1 and Sp600125 ,the expression of E‐cad was increased and the expres‐sion levels of α‐SMA ,ColⅠand p‐JNK were significantly decreased 72 h after treatment with TGF‐β1 and Sp600125 in inhibitor group. As compared with normal group ,the shape of the cells in inhibitor group was prolate ,and the expression of E‐cad ,α‐SMA ,ColⅠand p‐JNK was not significantly different.Conclusion JNK signaling pathway is related to the process of EMT of human alveolar epithelial cells induced by TGF‐β1. Sp600125 ,a special inhibitor of JNK ,could validly restrain the process.

Objective To analyse the current situation and advance in perioperative therapy of liver transplantation for primary hepatocellular carcinoma(HCC).Methods The published papers on current situation and advance in the perioperative therapy of liver transplantation for HCC were reviewed.Results The survival rate of liver transplantation for HCC in early stage has been the same as that for benign liver diseases up to now. However, it is still a difficult problem to improve the survival rate of liver transplantation for advanced HCC. The ideal perioperative therapies of liver transplantation for HCC should be helpful to suppress the growth of tumor while the HCC patients are waiting for donated livers, to diminish or eliminate the intraoperative spread or implantation of tumor cells and to repress the micrometastasis postoperatively. The current perioperative therapies of liver transplantation for HCC include hepatic arterial chemoembolization, systemic chemotherapy, radiotherapy, percutaneous ethanol injection into HCC and radiofrequency ablation etc. Conclusion The perioperative assistant therapy of HCC can not only save time for patients before liver transplantation but also improve the survival rate after operation.

At present the concept of clinical thinking is reviewed at the narrow senses and there are many limitations.So it is very necessary to understand the concept of clinical thinking at the broad senses.

Antiplatelet therapy and stent implantation have been the dominant treatment to reduce the mortality of patients with coronary artery disease.Recently,studies have showed that adverse cardiac events still occur in part of patients with coronary artery disease after the antiplatelet treatment with aspirin and/or clopidogrel.Thus,resistance to aspirin and clopidogrel has attracted increasing attention.It will be great benefit to these patients who were identified resistance and made tailoring antiplatelet therapy So far many platelet function tests has been used in clinical to monitor the reaction of the antiplatelet drugs for prevention and treatment of thrombosis in patients with coronary artery disease.These monitoring tests may be chosen based on different antiplatelet drugs including aspirin,clopidogrel and GP Ⅱ b/Ⅲ a antagonist.The results of antiplatelet drug resistance may be different due to different platelet function methods,thus the related clinical adverse events needs further verification.