OBJECTIVE:To evaluate the utilization of antimicrobials for breast malignant tumor in perioperative period. METHODS:Medical records of patients with breast malignant tumor in our hospital were analyzed statistically and retrospectively. RESULTS:100% of patients received antibacterial drugs preventively in perioperative period. Positive rate of administration time was 100%. 363 patients underwent operations over 3 hours were not given antimicrobials;the average time of application of antimicrobials in perioperative period was 90 h. The utilization ratio of first generation cephalosporins and penicillins was 55.1% and that of four categories was 41.1%,such as penicillin spiked with enzyme inhibitors,quinolones,third generation cephalosporins and second generation cephalosporins. 6 patients replaced antimicrobials unreasonably and 3 patients were given Azithromycin sodium chloride injection. CONCLUSION:The utilization of antibacterials in patients with breast malignant tumor in perioperative period is appropriate on the whole while there still are some problems.

OBJECTIVETo investigate the effect of allicin in inhibiting insulin-induced vascular smooth muscle cell (VSMC) proliferation and migration.

METHODThe tissue explant method was adopted to culture rat's aVSMCs, and the immunofluorescence method was used to identify α-SMA. Cells from the third to fifth generations were selected in the experiment The insulin-induced VSMC model was established. The experiment was carried out in five groups: the control group, the insulin group, the allicin group, the ERK inhibitors PD98059 group (20 μmol · L(-1)) and the PD98059 + allicin group. VSMCs' proliferation was determined by CCK8 colorimetric method, while its migration was detected by cell counting; The western blotting was used to detect total ERK, Phospho-ERK, PCNA protein's expression.

RESULTPrimary cultured VSMCs grew well in the spindle shape under the lightmicroscope, with peak and valley. α-SMA immunofluorescence results showed that the cultured cells had typical VSMCs' features. Insulin could stimulate VSMCs' proliferation and migration, with the best effect at the concentration of 100 nmol · L(-1). The pretreatment with allicin could significantly inhibit VSMCs' proliferation and migration induced by insulin in a dose-dependent manner. The pretreatment with PD98059 and allicin + PD98059 could inhibit VSMCs' proliferation and migration induced by insulin remarkably as well. Insulin could significantly accelerate VSMCs' expression of such proteins as p-ERK, PCNA. Contrarily, allicin could notably inhibit VSMCs' expression of such proteins as p-ERK, PCNA in a dose-dependent manner.

CONCLUSIONAllicin could significantly inhibit VSMCs' proliferation and migration induced by insulin, which may be related to the inhibition of the activation of ERK signal path.

Animals ; Cell Movement ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Insulin ; metabolism ; Male ; Muscle, Smooth, Vascular ; cytology ; drug effects ; metabolism ; Myocytes, Smooth Muscle ; cytology ; drug effects ; Plant Extracts ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Sulfinic Acids ; pharmacology

Objective To observe effect of Rho kinase inhibitor combined with hyperbaric oxygen on the recovery of neurological function after spinal cord injury in patients with thoracolumbar fractures.Methods A total of 80 cases of thoracolumbar fractures combined with spinal cord injury patients as the research object, the packet by the standards of admission of single and double serial number, odd numbers were divided into control group, even number were divided into treatment group (40 cases in each group); two groups underwent surgical treatment, control group was given single usage fasudil treatment, treatment group was given hyperbaric oxygen therapy for auxiliary therapy on the basis of control group, Continuous treatment for two months.Compared neurological function scoring, grading, blood superoxide dismutase (SOD) , epithelial growth factor (EGF) , lipid peroxide (LPO) and adverse reactions pre-and post-treatment between two groups of patients.Results After treatment,total effective rate of treatment group (92.50%), ASIA sensory, movement scores were significantly higher than control group (75.00%)(P<0.05);Content of SOD and EGF of treatment group were significantly higher than control group(P<0.05);the adverse reaction rate (12.50%) was significantly lower than that of control group (32.50%)(P<0.05).Conclusion Rho kinase inhibitor combined with hyperbaric oxygen can significantly improve the degree of injury, neurological function and blood SOD, EGF, LPO content in patients with lumbar vertebral fractures combined with spinal cord injury, and the adverse reactions are few, has definite clinical effect.

Objective　To examine the soluble protein patterns of three ordinary species of cockroaches in Anhui province by SDS-PAGE technique, and to find the method of scientific classification for cockroaches. Methods　The soluble protein liquid was made by the way of freezing and melting homogenate. SDS-PAGE was carried out in the liquid. After using Coomassie brilliant blue G250, the molecular weight of all of protein strips were measured and calculated by standard curve. Results　The numbers of female and male of Periplaneta fuliginosa were 31 strips and 29 strips; the numbers of female and male of Periplaneta Americana were 30 strips and 28strips; the numbers of female and male of Blattella germarica were 28 strips and 25 strips. The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect. Conclusion SDS-PAGE can be used as one of classification methods for cockroaches.

Objective To observe the clinical efficacy of esomeprazole combined with trimebutine maleate in the treatment of reflux esophagitis patients without helicobacter pylori infection.Methods 78 patients with reflux esophagitis were randomly divided into two groups,46 patients in study group and 32 patients in control group.30 minutes before the breakfast,all the patients took Nexium 20mg,twice a day.The study group was given trimebutine maleate 200mg,thrice a day additionally.The treatment course was 4 weeks.The effect and the result of gastroendoscopy were observed.Results The average time of clinical symptoms disappeared in study group was (10.51 ± 2.43) days,which was significantly shorter than (12.31 ± 3.17) days of the control group (t-2.84,P ＜ 0.01).The total effective rate of the study group was 97.83％,which was significantly higher than 81.25％ of the control group (x2 =4.48,P ＜ 0.05).Conclusion Esomeprazole combined with trimebutine maleate in the treatment of reflux esophagitis without helicobacter pylori infection can promote gastric emptying,prevent reflux,control esophageal inflammation and promote ulcer healing effectively.

Hemoglobinopathy is a kind of hereditary hematonosis caused by the disproportion among normal hemoglobins or formation of abnormal hemoglobins.Hemoglobin analysis plays an important role in screening and diagnosing hemoglobinopathy.Emerging methods have been applied continuously to hemoglobinometry since the early 20th century.Nowadays,agarose gel electrophoresis,high performance liquid chromatography and capillary electrophoresis are widely used with mass spectrometry as the latest applied method.All these methods have their own features.Appropriate combination of these methods will lead to more scientific and reliable results.

Objective To explore the incidence of the carotid artery atherosclerotic plaque in patients with cerebral infarction(CI).Methods 138 CI patients on their carotid artery were examed with color doppler ultrasonography.The incidence,stability and location of the carotid artery atherosclerotic plaque in CI patients were observed and compared with those of the normal controls.Results 120 cases(87.7%) of the 138 CI patients were turned out 175 atherosclerotic plaques.36 cases of the 140 normal controls were turned out to be with atherosclerotic plaque(25.7%).There was significant difference between the two groups(P

Biomarkers ; analysis ; Environmental Monitoring ; methods ; Humans ; Occupational Exposure ; analysis ; Xylenes ; analysis

In order to figure out the present situation of personnel training to young scientific talents in basic research fields in China,we managed to know national natural science fund in each province from management systems,focusing on policy-making trends and new methods of cultivation to young scientific talents,for the purpose of analyzing present situation and summarizing the characteristics and developmental trends of personnel training to young scientific talents in each province.