Characterizations of the VP4 (P type) and VP7 (G type) genes of Korean isolates of bovine rotavirus were performed using RT-PCR/RFLP and nucleotide sequencing analysis. After RT-PCR amplification of partial length (1094bp) of the VP4 and full length (1062bp) of the VP7 genes, amplified PCR products were digested with restriction endonucleases and digestion patterns were compared with those of reference rotaviruses. With the VP4 genes, four RFLP (AD) profiles were observed; three (A, B and C) were the same as those of bovine rotavirus NCDV (P[1]), IND (P[5]) and B223 (P[11]), respectively, Profile D was the same as that of porcine rotavirus OSU (p[7]). With the VP7 genes, five RFLP profiles (I-V) were observed; three of them (1, II and III) were the same as those of bovine rotavirus NCDV (G6), Cody I-801 (G8), and B223 (G10), respectively, Profile IV and V were atypical to those of reference bovine rotaviruses used in this study. These two profiles were identified as G6 and G5, respectively, after analyzing and comparing the nucleotide sequences. The G typing analysis revealed that 61.9% (26/42) were G6, which included G6 subtype; 28.6% (12/42) were G5; 7.1% (3/42) were G10; 2.4% (1/42) were G8. The P typing analysis revealed that 54.8% (23/42) were P(5); 28.6% (12/42) were P(7); 11.8% (5/42) were P(11); 4.8% (2/42) were P(1). Our results showed that G6/P(5) were the most prevalent rotaviruses in diarrheic calves in Korea. Also, this is the first report that G5P(7) rotaviruses were identified from cattle with diarrhea.
Animals ; Base Sequence ; Cattle ; Diarrhea ; Digestion ; DNA Restriction Enzymes ; Korea ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length* ; Rotavirus*

Using atomic absorption spectrometry Pb, Cd, Cu, Zn and Fe were measured in sweat and Pb, Cd, Cu and Zn, in blood. The subjects were 22 healthy males, aged between 23-44. Sweating was induced with an environmental room (1 hour at 46C dry bulb and 36C wet bulb). Sweat samples were obtained from the left arm by the armbag technique. The sweat volume was 23.3+7.3ml(n= 22). The concentrations, in ug/ml, of trace elements in sweat were : lead, 0.18+0.17(n=22); cadmium, 0.006+0.006(n=22); copper, 0.37+0.66(n=10); zinc, 0.63+0.5(n=10); and iron, 0.22+0.12(n=10). The concentrations, in ug/ml, of trace elements in blood were : lead, 0.28+0.15(n=20); cadmium, 0.005+0.007 (n=20); copper,0.69+0.22(n=20); and zinc,5.61+0.86(n=20). These results clearly showed the significance of sweating as a route of excretion of trace element.
Absorption ; Arm ; Cadmium ; Copper ; Humans ; Iron ; Male ; Spectrum Analysis ; Sweat* ; Sweating ; Trace Elements ; Zinc

No abstract available.
Ossification of Posterior Longitudinal Ligament* ; Spine*

Neonatal meningitis caused by the Group B beta-hemolytic streptococcus was first reported in 1958. In recent years this organism has assumed major importance in the etiology of neonatal infection. In Korea, this organism is likely to play an increasing role in neonatal infection. So this investigation was designed to report the 9 cases of Group Bbeta-hemolytic streptococcus infected neonates admitted to the NICU, department of Pediatrics, Hanil Hospital from January 1990 to June 1993. The results were as followings. 1) Onset of disease was between 12days and 32 days of age and male to female ratio is 1:2. 2) Two cases were delivered by c-section, seven cases were by NFSD and all of the infants had normal birth weight with full term at delivery. Maternal obstetric and neonatal complications were not detected in all cases. 3) The common presenting symptoms were fever in all cases, seizure in 6 cases, feeding difficulty, lethargy, vomiting, etc. 4) Group Bbeta-hemolytic streptococcus was isolated in CSF of all cases and in blood of 6 cases. 5) There were abnormal brain CT findings in 4 cases of group Bbeta-hemolytic streptococcal meningitis. 6) The sensitive antibiotics were Cefotaxime, Cefazoline, Erythromycin, Ampicillin, Chloramphenicol, Clindamyucin and Penicillin. 7) All cases were reconvered except one case that expired on the 1st day of admission and 6 cases were followed up for the period of 2months to 33months without neurologic sequelae, of which 1 case revealed decreased wave on Lt. hemisphere in ABR.
Ampicillin ; Anti-Bacterial Agents ; Birth Weight ; Brain ; Cefazolin ; Cefotaxime ; Chloramphenicol ; Erythromycin ; Female ; Fever ; Humans ; Infant ; Infant, Newborn ; Korea ; Lethargy ; Male ; Meningitis* ; Pediatrics ; Penicillins ; Seizures ; Streptococcus ; Vomiting

20-year-old patient presented with the episodes of generalized hyperkeratotic lesions with bullae since her early life, without family history. Histopathological examination by light and electron microscopes showed the characteristic features of epidermolytic hyperkeratosis. Primarily, she failed to respond to the treatment with propylene glycol. Vitamine A palmitate(A-Mulsin) per os appears to be a beneficial remedy for epidermolytic hyperkeratosis, although its availability is limited due to the side effects on a long term therapy. Repeated biopsies in the normal appearing lesions 2 months after treatment of vitarnin .A palmitate showed a substantial reduction of the horny layer on the light microscope and orderly arrangement of the tonofilaments, and properly formed keratohyaline granules on EM, but the underlying disorder of keratinization remained unchanged. Treattnent of 2 months with vitamin A was interrupted by side effects of nasal bleealing, chelitis and xerosis.
Biopsy ; Humans ; Hyperkeratosis, Epidermolytic* ; Intermediate Filaments ; Propylene Glycol ; Vitamin A* ; Vitamins* ; Young Adult

No abstract available.
Glomerulonephritis, IGA* ; Immunity, Cellular* ; Immunoglobulin A*

No abstract available.
Glomerulonephritis, IGA* ; Immunity, Cellular* ; Immunoglobulin A*

Since the recognition that certain conditions such as allergic rhinitis, asthma and urticaria are due to the substances which are innocuous to the individuals, it has been a common practice to establish a diagnosis by reexposure of the individual to the suspected subsutance. Their pathogenesis was classified into immunologic and nonimmunologic mechanism. The immunologic mechanism in such conditions is mainly due to interaction between antigen and IgE skin-sensitizing antibody bound to tissue mast cells. There are two approaching methods in evaluating allergic disease. One is in vitro test which includes R.A.S.T. and leukocukocyte histamine release test and the other method is in vivo test which includes provocation test and skin tests. Prick test, a kind of skin test, is most frequently used in clinical procedure because of its simplicity and safety of appliation and better correlation with clinical history as well as specificity and highly reproducibility. It is well accepted that the therapeutic principle in allergic disease is to find out the causative allergens and get rid of them from patient's environment. If the causative allergens are inavoidable, the patient should be treated by means of specific hyposensitization. In evaluating allergic disease, the patient's clinical history is most important. (countinued..)
Allergens ; Asthma* ; Diagnosis ; Histamine Release ; Humans ; Immunoglobulin E ; Mast Cells ; Rhinitis* ; Sensitivity and Specificity ; Skin Tests ; Urticaria

It had heen emphasized the necessity for upper gastrointestinal endoscopic examinations in patients. Who has had gastrectomy and presents persisting gastrointestinal symptoms. This study was conducted to evaluate endoscopic findings and clinical symptoms in patients following gastrectomy. (continue...)
Gastrectomy* ; Humans

BACKGROUND: Allergic diseases are characterized by immediate - and late - phase reactions to various allergens by the selective activation of a subset of CD4 + T cells. In response to allergen, T cells isolated from atopic donors are biased to low levels of IFN - y and high levels of IL - 4, and vice versa by T cells from non - atopic donor. Objective : The aim of this study was to evaluate the patterns of IL - 4 and IFN - y production after Der p I stimulation and the effect on the cytokine production from T cells by budesonide, disodium cromoglycate and cyclosporin A in atopic and non - atopic individuals. MATERIAL AND METHOD: Seven Der p I specific atopic and 7 non - atopic individuals were selected. We decided the 50% inhibiting concentration of each immunomodulator by lymphocyte proliferation assay, and measured their effects on the cytokine production in vitro by intracellular IL - 4, IFN - y staining and flow cytometry. Results and CONCLUSION: There was significant difference on stimulation index ( SI ) of production of IFN - y as well as IL-4 after Der p I stimulation between atopic and non-atopic individuals ( IL - 4 ; 1.57 +/- 0.7 : 0.98 +/- 0.2, p = 0.026, IFN - r : 1.45 +/- 0.5 : 0.95 +/- 0.2, p = 0.048 ). The synthesis of IL - 4, and IFN - r were significantly inhibited after the stimulation of every immunomodulators in atopic individuals, DSCG couldnt inhibit IL - 4 and IFN - r in nonatopic individuals. There was no significant difference in the inhibiting effect of these immunomodulators in both of them.
Allergens ; Bias (Epidemiology) ; Budesonide ; Cromolyn Sodium ; Cyclosporine ; Dust* ; Flow Cytometry ; Humans ; Immunologic Factors ; Interferons* ; Interleukin-4 ; Lymphocytes ; Pyroglyphidae* ; T-Lymphocytes* ; Tissue Donors