After an analysis of the application of medical video resources in academic conference , training of medical skills and health education of patients , the experiences in development of medical video resources in our li-brary were described.The characteristic medical video resources database was developed by establishing a naviga-tion system for free medical video resources on networks, introducing the mature academic video resources databases and collecting the medical video resources in our hospital .Service for medical video resources was popularized in forms of video reading and catalogue pushing .

BACKGROUND:Mesenchymal stem cel s can be used to treat a variety of injuries, but little is known about their effect on systolic hypertension. OBJECTIVE:To study the therapeutic effect of bone marrow mesenchymal stem cel s on systolic hypertension rats. METHODS:Twenty-four rats were randomized equal y into control, model and cel transplantation groups. A rat model of systolic hypertension was made by injection of vitamin K and warfarin sodium in the model and cel transplantation groups. Rats in the cel transplantation group were given injection of bone marrow mesenchymal stem cel s into the left ventricle, while those in the other two groups given normal saline. RESULTS AND CONCLUSION:Compared with the model group, bone marrow mesenchymal stem cel s could reduce left ventricular mass index, increase cardiac tissue type I col agen volume fraction, col agen volume fraction and perivascular col agen volume fraction, but the ratio of end-diastolic volume/body weight did not change significantly. These findings indicate that bone marrow mesenchymal stem cel transplantation can reduce myocardial hypertrophy and cardiac fibrosis in systolic hypertension rats.

Objective To detect the expression of Sushi Repeat Containing Protein X Linked 2 (SRPX2) in hunan endometrial carcinoma (EC) and its role in cell proliferation and apoptosis Methods Immunohistochemistry was applied to detect the expression of SRPX2 in EC and matched tumor-adjacent tissues (n =50).The correlation between SRPX2 expression and clinical features was analyzed by Pearson chi-square test.siRNA was used to down-regulate the expression of SRPX2 in HEC-1A cells.The effects of SRPX2 on cell proliferation and apoptosis were measured by CCK-8 assay and Flow cytometer,respectively.The FAK and Cyclin D1 expression were detected after silencing SRPX2 expression.Results The expression of SRPX2 was significantly up-regulated in EC tissues,and the high expression of SRPX2 was associated with the large tumor size (＞3 cm) and advanced FIGO stage (Ⅲ+Ⅳ stage).Silencing SRPX2 could inhibit cell proliferation,induce cell apoptosis and repress FAK and Cyclin D1 expression in HEC-1A cells.Conclusion The expression of SRPX2 is significantly higher in EC tissues,which is closely related with cell proliferation.Scilencing SRPX2 could achieve an anti-cancer role through inhibiting cell proliferation and inducing cell apoptosis by the down-regulation of SRPX2.

Objective To study the clinical efficacy of thermotherapy combined chemotherapy in the treatment of the advanced non-small cell lung carcinoma with neuroendocrine differentiation (NSCLC-NED).Methods The clinical data of 16 patients with NSCLC-NED were retrospectively reviewed.To analyzed the efficacy and survival of these patients.All patients were treated by thermotherapy with the combined chemotherapy regiment of platinum agents.Results There were 7 partial responses and 4 stable disease,The overall response rate (RR) was 43.8％.The one year survival rate was 37.5％ (6/16).The improvement rate of quality of life was 62.5％ (10/16).Conclusion Neuroendocrine differentiation is an important indicator of biological behavior of NSCLC.Thermochemotherapy is an active regimen in the advanced NSCLC-NED with an acceptabe toxicity.

The incidence of allergic airway diseases has grown steadily in the past decade.Allergic rhinitis is closely associated with the development of asthma.Genetic and environmental factors are etiologically considered to be the main contributors to the development of allergic airway diseases.So far,overwhelming data from studies on humans and animals have indicated a systemic pathway linking the upper and lower airways.Neurogenic inflammation plays an important role in allergic airway diseases.This paper outlines the latest progress in the related researches.

Objective To investigate the role of Wnt/β-catenin signaling pathway in migration ability of asthmatic rat airway smooth muscle cells (ASMCs).Methods The asthmatic rat airway smooth muscle cells were cultured in vitro.The expression of Wnt/β-catenin in ASMCs was detected by RT-PCR and Western blot.Migration ability of ASMCs was detected by wound healing assay and transwell migration assay.Results Compared with normal rats,the expression level of Wnt/β-catenin in chronic asthmatic rats ASMCs was obvious higher.The β-catenin signaling pathway by specific siRNA could reduce beta-catenin mRNA and protein expression level of ASMCs.The knockdown of Wnt/β-catenin was able to decreases the migration ability of ASMCs.Conclusion Abnormal activation of Wnt/β-catenin signaling pathway may participants in the migration ability of ASMCs of chronic asthma rats.

Objective:To investigate the effect of early postoperative cognitive dysfunction and postoperative analgesia situation after intercostal nerve block was applied on elderly patient received thoracic surgery.Methods: 105 elderly patients underwent thoracic surgeries were divided into observation group (35 patients received intercostal nerve block combined with general anesthesia), control A group (35 patients received epidural anesthesia combined with general anesthesia) and control B group (35 patients received routine general anesthesia). The cognitive function, postoperative pain, intraoperative mean artery pressure (MAP) and heart rate of the patients among different groups were respectively compared.Results: The cognitive function scores in postoperative 12h, 24h, 72h of observation group were significantly higher than that of control A group (t=20.917,t=27.780, t=74.081,P<0.05), respectively. And all of these data also were significantly higher than that of control B group (t=37.922,t=48.969,t=62.653,P<0.05), respectively. The differences of MAP, HR value between observation group and control A group were statistically significant (t=18.927,t=22.380,P<0.05), respectively. And the differences of them between observation group and control B group also were statistically significant (t=31.051, t=19.932, P<0.05), respectively. Besides, the differences of pain scores in postoperative 6h, 12h, 24h, 48h between observation group and control A group were not statistically significant, while all of pain scores of observation group were significantly lower than that of control B group (t=18.731,t=19.035, t=21.093,t=17.036;P<0.05).Conclusion:Intercostal nerve block combined with general anesthesia can ensure more stable intraoperative vital signs for elderly patients underwent thoracic surgeries, and it contributes to improve early cognitive function and possesses better postoperative analgesic effect for elderly patients.

Objective: To research the effect of dexmedetomidine on regional cerebral oxygen saturation(rSO2)and postoperative cognitive function in elder patients received prostatectomy by using laparoscope. Methods: 60 elderly patients received prostatectomy by using laparoscope were divided into observation group (30cases) and control group (30cases) as random number table. In observation group, the dexmedetomidine was injected by venous pump after endotracheal intubation of general anesthesia was implemented, while the equivalent normal saline (NS) was injected by venous pump in control group. The rSO2, PaCO2 and PaO2 of different time point between the two groups were observed and compared. The usages of propofol and remifentanil, the situation of postoperative restlessness, and situation of nausea and vomiting between the two groups were compared. Besides, the scores of MoCA of different time point between the two groups also were compared. Results: The usages of propofol and remifentanil of observation group were significantly lower than that of control group (t=3.449, t=9.466, P<0.05), respectively. The occurrence rate of postoperative restlessness and occurrence rate of nausea and vomiting were 10.0% and 6.7%, respectively, while they were 13.3% and 3.3%in control group, and the differences of them between the two groups were no significant (x2=0.162, x2=0.351, P>0.05). The score of MoCA of observation group was significant higher than that of control group (t=8.938, P<0.05) at postoperative 1d. Conclusion: In the elder patients received prostatectomy by using laparoscope, the dexmedetomidine doesn't affect the rSO2 level of patients, while it contributes to fast recover the postoperative cognitive function of patients.

The hyperamplification in centrosomes give rise to a series of biochemical events including formation of multipolar spindle, abnormal segregation of chromosome and aneuploid, promoting the performance of chromosome instability, which is the main pathogenesis of multiple human malignancies.STIL participates in the formation of centrioles, activates CDK1/CyclinB1 complex and promotes mitotic entry.Moreover, it regulated expression of related downstream genes through Sonic Hedgehog signal pathway.Thus, STIL correlates with gastric and pancreatic carcinogenesis and progression.We aim to review the structure and function of STIL, association with malignancies and its potential mechanism.