1.Overexpression of cyclin D1 and cyclin E in 1,2-dimethylhydrazine dihydrochloride-induced rat colon carcinogenesis.
Kwon HUR ; Jung Rae KIM ; Byung Il YOON ; Jung Keun LEE ; Jae Hoon CHOI ; Goo Taeg OH ; Dae Yong KIM
Journal of Veterinary Science 2000;1(2):121-126
Deregulation of G1 cyclins has been reported in several human and rodent tumors including colon cancer. To investigate the expression pattern of G1 cyclins in 1,2- dimethyl-hydrazine dihydrochloride (DMH)-induced rat colon carcinogenesis, we studied the expression of cyclin D1 and cyclin E by quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry (IHC). The mRNA level of cyclin D1 was increased 1.2-fold in adenocarcinomas but not significantly in adenomas, when compared with normal rat colonic mucosa (p<0.05). The cyclin E mRNA level was increased 2.7-fold in adenomas and 3.3-fold in adenocarcinomas (p<0.05). The PCNA mRNA level was also increased 1.9-fold in adenomas and 1.8-fold in adenocarcinomas (p<0.05). Immunohistochemical staining revealed exclusive nuclear staining of the neoplastic cells for cyclin D1, cyclin E and PCNA. Cyclin D1 expression was detected in 56.3% of the adenomas and in 61.5% of the adenocarcinomas examined, whereas cyclin E expression was detected in 87.5% of the adenomas and in 92.3% of the adenocarcinomas. Overall, cyclin D1, cyclin E and PCNA expression was significantly increased at both the mRNA and protein levels in normal colonic mucosa, adenomas and adenocarcinomas, but there was no significant difference in the degree of expression of these genes in adenomas and adenocarcinomas. Our results indicate that the overexpression of cyclin D1 and cyclin E may play an important role during the multistage process of rat colon carcinogenesis, at a relatively early stage, and may disturb cell-cycle control in benign adenomas, and thereafter, participate in tumor progression.
1,2-Dimethylhydrazine/toxicity
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Adenocarcinoma/*chemically induced/metabolism
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Adenoma/*chemically induced/metabolism
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Animals
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Carcinogens/toxicity
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Cell Cycle/drug effects/physiology
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Colon/metabolism
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Colonic Neoplasms/*chemically induced/metabolism
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Cyclin D1/*biosynthesis/genetics
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Cyclin E/*biosynthesis/genetics
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Gene Expression Regulation, Neoplastic
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Immunohistochemistry
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Male
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Proliferating Cell Nuclear Antigen/biosynthesis/genetics
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RNA, Messenger/metabolism
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Rats
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
2.B-Natriuretic Peptide (BNP) Assay for Diagnosis of Congestive Heart Failure.
Won KIM ; Won Young KIM ; Yong Taeg JUNG ; Kyoung Soo LIM ; Yun Kyung PARK
Journal of the Korean Society of Emergency Medicine 2003;14(5):624-629
PURPOSE: Rapid diagnosis of congestive heart failure (CHF) is important for prompt and appropriate treatment, but is often difficult. The B-type natriuretic peptide (BNP) has been described as a powerful diagnostic test for heart failure. The goal of this study was to determine the diagnostic cutoff values of BNP, to evaluate useful factors for the diagnosis of CHF, and to develope a diagnostic algorithm for CHF with acute dyspnea patients. METHODS: BNP levels were obtained in 721 patients presenting to the emergency medical center with acute dyspnea from January 2001 to December 2002. With the use of inclusion criteria, 305 patients were excluded. Blood samples were collected by venipuncture and were analyzed by using Triage BNP assay. Included patients underwent echocardiography to evaluate the left ventricular (LV) dysfunction. RESULTS: The mean BNP concentration was higher in patients with CHF (n=214) than in those with other diagnose (517.31+/-37.70 pg/mL vs 75.53+/-11.25 pg/mL, p<0.001). At a blood concentration of 100 pg/mL, BNP showed a sensitivity of 79 %, a specificity of 83 %, a positive predictive value of 83%, and a negative predictive value of 79%. CONCLUSION: The BNP measurement is a useful tool in the diagnosis of CHF in patients presenting with dyspnea. We propose a diagnostic algorithm for CHF based on the BNP assay.
Diagnosis*
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Diagnostic Tests, Routine
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Dyspnea
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Echocardiography
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Emergencies
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Estrogens, Conjugated (USP)*
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Heart Failure*
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Humans
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Natriuretic Peptide, Brain
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Phlebotomy
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Sensitivity and Specificity
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Triage
3.A Case of Gastric Adenoma Incidentally Found on PET-CT.
Kyoung Taeg KONG ; Ho Jung KIM ; Yong Whee BAHK ; Kwang Seok KIM ; Sang Chul JEE ; Jeong Wook KIM ; Dae Han CHOI ; Sung Hwan KANG ; Hwoon Yong JUNG
Nuclear Medicine and Molecular Imaging 2007;41(5):373-376
We report a case of gastric adenoma which was found incidentally on 18F-FDG PET/CT study for cancer screening in asymptomatic patient. It showed focal and intensely increased FDG uptake in the antrum of stomach. On the gastroduodenoscopy, it showed flat elevated lesion with irregular margin. Histologically, the lesion was confirmed gastric adenoma with high grade dysplasia and removed by endoscopic mucosal resection.
Adenoma*
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Early Detection of Cancer
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Fluorodeoxyglucose F18
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Humans
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Positron-Emission Tomography and Computed Tomography
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Stomach
4.Osteochondromas of the Bilateral Tali.
Chan Ha HWANG ; Shin Taeg KANG ; Bo Hyun KIM ; Jae Yong BYUN ; In Ho JUNG ; Hyang Mi SHIN ; Dae Joong KIM
Journal of Korean Foot and Ankle Society 2006;10(1):113-116
Benign and malignant tumors are found in the foot, although the incidence is low. The most common bone tumor in the foot is osteochondroma, which is thought to develop in bones that form through the process of enchondral ossification. In particularly, osteochondromas in the foot mostly occur in metatarsal bones and phalanges. It is seldom found in talus. It is usually confused with osteophyte or enthesis. We report an osteochondroma case confirmed by roentgenographical and pathological investigation, after removing the masses form bilateral tali of a 19-year-old male patient presented with bilateral ankle joint pain for three years.
Ankle Joint
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Foot
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Humans
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Incidence
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Male
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Metatarsal Bones
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Osteochondroma*
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Osteophyte
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Talus
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Young Adult
5.Nonleukemic Granulocytic Sarcoma in the Bile Duct: A Case Report.
Hyun Woo KIM ; Seong Jun CHOI ; Je Hwan LEE ; Jung Hee LEE ; Taeg Soo KIM ; Yong Gil KIM ; Jeong Min KANG ; Jooryng HUH ; Kwang Min PARK ; Kyoo Hyung LEE
Journal of Korean Medical Science 2006;21(4):745-748
Granulocytic sarcoma (GS) is an extramedullary tumor composed of immature myeloid cells, typically occurring during the course of acute myelogenous leukemia. Non-leukemic GS, that is, GS with no evidence of overt leukemia and no previous history of leukemia, is very rare, and even more unusual is nonleukemic GS of the bile duct. We report a case of nonleukemic GS of the bile duct. The patient was initially misdiagnosed as a bile duct carcinoma arising in the hilum of the liver (so-called Klatskin tumor), and received a right lobectomy of the liver. Histological examination of the tumor yielded the diagnosis of GS, and the bone marrow biopsy did not show any evidence of leukemia. Considering the risk of subsequent development of overt leukemia, the patient was treated with two cycles of combination chemotherapy as used in the cases of acute myelogenous leukemia. To date, he has remained free of disease 15 months after treatment.
Tomography, X-Ray Computed/methods
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Sarcoma, Granulocytic/*diagnosis/metabolism/radiography
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Radiography, Abdominal
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Peroxidase/analysis
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Male
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Immunohistochemistry
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Humans
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Diagnosis, Differential
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Bile Ducts/chemistry/pathology
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Bile Duct Neoplasms/*chemically induced/metabolism/radiography
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Antigens, CD45/analysis
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Adult
6.A case of recurred gastric dysplasia associated with eosinophilic gastroenteritis.
Sang Chul JEE ; Hae Kyung KIM ; Kyoung Taeg KONG ; Yong Ju LEE ; Kee Myoung JUNG ; Jeong Ouk KIM ; Tae Hyun KIM
Korean Journal of Medicine 2007;73(5):530-534
The association of malignant tumors and peripheral eosinophilia or tissue eosinophilic infiltration has been uncommonly described. Moreover, a recent study has demonstrated some gastric cancers can express eosinophilic chemotactic factor. Interestingly, we recently experienced a case of recurrent eosinophilic gastroenteritis with relapsing gastric dysplasia. It is suggested that gastric dysplasia can also produce eosinophilic chemotactic factors and eosinophilic gastroenteritis can develop with recurred dysplasia. In this patient, eosinophilia served as an indicator of disease activity or as a marker. Eosinophilia may represent an important disease marker with prognostic significance and may rarely cause disease on its own.
Chemotactic Factors
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Eosinophilia
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Eosinophils*
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Gastroenteritis*
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Humans
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Stomach Neoplasms
7.Clinical characteristics of constipation associated with diabetes mellitus.
Taeg Soo KIM ; Jeong Sik BYEON ; Seung Jae MYUNG ; Suk Kyun YANG ; Jung Eun KO ; Oh Ryoun KWON ; In Ja YOON ; Jeong Min KANG ; Hyun Woo KIM ; Jung Min AHN ; Soon Joo KIM ; Ji Yun JO ; Kee Don CHOI ; Gin Hyug LEE ; Hwoon Yong JUNG ; Weon Seon HONG ; Jin Ho KIM
Korean Journal of Medicine 2006;70(5):527-534
BACKGROUND: Constipation occurs frequently in diabetes mellitus (DM). However, there are few reports that investigated the characteristics of constipation associated with DM. The purpose of this study was to evaluate the clinical features of constipation associated with DM. METHODS: Among constipated patients who visited Asan Medical Center from January 2000 to December 2004, 45 patients with DM (DM group) and 104 patients without DM (non-DM group) were included in this study. We reviewed the clinical presentation, results of anorectal manometry, colon transit time study, and defecogram. We also analyzed the response to biofeedback therapy. RESULTS: The severity of constipation symptoms before treatment was not different between DM and non-DM group. Patients with colon transit time over 56 hours were more frequent in DM group than in non-DM group (21/45, 46.7% vs. 31/104, 29.8% ; p=0.047). Among DM group, colon transit time and the duration of DM showed positive correlation (r=0.431, p=0.003). The resting anal sphincter pressure was significantly lower in DM group than in non-DM group (43.5+/-21.5 mmHg vs. 51.7+/-22.6 mmHg ; p=0.048). The results of defecography were similar between DM and non-DM group. Successful responses to biofeedback therapy were not different between DM and non-DM group (19/34, 55.9% vs. 43/79, 54.4% ; p=0.887). CONCLUSIONS: Slow transit constipation was more frequent in DM group than in non-DM group. The successful responses to biofeedback therapy appear to be similar between DM and non-DM group.
Anal Canal
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Biofeedback, Psychology
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Chungcheongnam-do
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Colon
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Constipation*
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Defecography
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Diabetes Mellitus*
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Humans
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Manometry
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Time and Motion Studies
8.Laparoscopic total extraperitoneal hernia repair of fallopian tube indirect inguinal hernia in reproductive aged woman: a case report.
Yong Hee PARK ; Eun Jung JUNG ; Jung Mi BYUN ; Min Sung AN ; Young Nam KIM ; Kyung Bok LEE ; Moon Su SUNG ; Ki Tae KIM ; Eun Taeg KIM ; Chul Hoi JEONG ; Dae Hoon JEONG
Obstetrics & Gynecology Science 2017;60(6):608-611
An indirect inguinal hernia containing the fallopian tube alone is extremely rare in reproductive-aged women without any genital tract anomalies. Despite this rarity, early diagnosis and adequate management is important to prevent strangulation and recurrence. We present a case of an indirect inguinal hernia containing only the fallopian tube in the hernia sac, which was successfully reduced by using a laparoscopic total extraperitoneal approach and repaired with a polypropylene mesh.
Early Diagnosis
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Fallopian Tubes*
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Female
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Hernia*
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Hernia, Inguinal*
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Herniorrhaphy*
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Humans
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Laparoscopy
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Polypropylenes
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Recurrence
9.Ginkgo biloba extract (GbE) enhances the anti-atherogenic effect of cilostazol by inhibiting ROS generation.
In Hyuk JUNG ; You Han LEE ; Ji Young YOO ; Se Jin JEONG ; Seong Keun SONN ; Jong Gil PARK ; Keun Ho RYU ; Bong Yong LEE ; Hye Young HAN ; So Young LEE ; Dae Yong KIM ; Hang LEE ; Goo Taeg OH
Experimental & Molecular Medicine 2012;44(5):311-318
In this study, the synergistic effect of 6-[4-(1-cyclohexyl-1H-tetrazol-5-yl) butoxy]-3,4-dihydro-2(1H)-quinolinone (cilostazol) and Ginkgo biloba extract (GbE) was examined in apolipoprotein E (ApoE) null mice. Co-treatment with GbE and cilostazol synergistically decreased reactive oxygen species (ROS) production in ApoE null mice fed a high-fat diet. Co-treatment resulted in a significantly decreased atherosclerotic lesion area compared to untreated ApoE mice. The inflammatory cytokines and adhesion molecules such as monocyte chemoattractant-1 (MCP-1), soluble vascular cell adhesion molecule-1 (sVCAM-1), and VCAM-1 which can initiate atherosclerosis were significantly reduced by the co-treatment of cilostazol with GbE. Further, the infiltration of macrophages into the intima was decreased by co-treatment. These results suggest that co-treatment of GbE with cilostazol has a more potent anti-atherosclerotic effect than treatment with cilostazol alone in hyperlipidemic ApoE null mice and could be a valuable therapeutic strategy for the treatment of atherosclerosis.
Animals
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Apolipoproteins E/genetics/physiology
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Atherosclerosis/*drug therapy
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Cytokines/metabolism
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Disease Models, Animal
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Drug Synergism
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Ginkgo biloba/*chemistry
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Humans
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Macrophages/cytology/drug effects
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Male
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Mice
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Mice, Nude
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Plant Extracts/*administration & dosage/chemistry
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Reactive Oxygen Species/*metabolism
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Tetrazoles/*administration & dosage
10.Soluble factor from tumor cells induces heme oxygenase-1 by a nitric oxide-independent mechanism in murine peritoneal macrophages.
Sang Wook KIM ; Hyun Mee OH ; Beom Su KIM ; Hun Taeg CHUNG ; Weon Cheol HAN ; Eun Cheol KIM ; Tae Hyeon KIM ; Geom Seog SEO ; June Hyung LYOU ; Yong Ho NAH ; Jae Chang JUNG ; Suck Chei CHOI ; Chang Duk JUN
Experimental & Molecular Medicine 2003;35(1):53-59
Tumor target-derived soluble secretary factor has been known to influence macrophage activation to induce nitric oxide (NO) production. Since heme oxigenase-1 (HO-1) is induced by a variety of conditions associated with oxidative stress, we questioned whether soluble factor from tumor cells induces HO-1 through NO-dependent mechanism in macrophages. We designated this factor as a tumor-derived macrophage-activating factor (TMAF), because of its ability to activate macrophages to induce iNOS. Although TMAF alone showed modest activity, TMAF in combination with IFN-gamma significantly induced iNOS expression and NO synthesis. Simultaneously, TMAF induced HO-1 and this induction was slightly augmented by IFN-gamma. Surprisingly, however, induction of HO-1 by TMAF was not inhibited by the treatment with the highly selective iNOS inhibitor, 1400 W, indicating that TMAF induces the HO-1 enzyme by a NO-independent mechanism. While rIFN-gamma alone induced iNOS, it had no effect on HO-1 induction by itself. Collectively, the current study reveals that soluble factor from tumor target cells induces HO-1 enzyme in macrophages. However, overall biological significance of this phenomenon remains to be determined.
Animals
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Antineoplastic Agents/pharmacology
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Bladder Neoplasms/metabolism/pathology
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Cell Line
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Drug Interactions
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Gene Expression Regulation, Enzymologic/drug effects
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Heme Oxygenase (Decyclizing)/analysis/*genetics
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Human
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Interferon Type II/pharmacology
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Macrophage Activation/drug effects
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Macrophages, Peritoneal/*metabolism
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Mice
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Mice, Inbred C57BL
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Nitric Oxide/biosynthesis/*metabolism
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Nitric-Oxide Synthase/genetics/metabolism
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Nitrites/analysis
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Tumor Cells, Cultured