Objective To evaluate an ideal way to prepare the extracellular matrix of urethra. Methods An orthogonal design [L9(34)] was used in the experiment.Urethras were obtained from 37 rabbits,among which 27 segments were randomly selected and were decellularized following the orthogonal design in 9 groups.The whole experiments were repeated for 3 times.After the decellularization process,the acellularity of the ECM was examined by haematoxylin-eosin staining.The optimum way was found out through comparing the numbers of the remained cellular elements by computer image analysis.An ideal way was found by statistic analysis.Then the ECM was obtained from 10 pieces of urethras by the optimum methods.The scanning electronic microscopy was used to confirm the decellulary matrix.Subsequently,the ECM was used as a graft for replacement. In 10 rabbits,the urethral defect were replaced with the urethral ECMs. At sacrifice,10 days,3 weeks,6 weeks and 24 weeks,the grafts was taken out,and the regeneration was confirmed by the haematoxylin-eosin staining. Results ECM resulting from different dedellularization process in the urethras are different in the numbers of remaining cellular elements.There are no cellular elements in the 7th and the 9th group of the tissues.The cellular elements was not found by the scanning electronic microscopy in the ECM getting from the optimum methods.In the animals with replacement,histologic examination showed complete regeneration 24 weeks post operation. Conclusions The best way to prepare the ECM of urethra is A 3B 2C 3.

Objective To investigate arterial blood gas(ABG)changes induced by different intra- abdominal pressures during CO_2 pneumoperitoneum in rabbits with lung and liver impact injury under controlled hemorrhage.Methods In this study 75 rabbits were randomized into nine groups(6 rabbits in each group)according to the amount of blood loss(6 ml/kg、12 ml/kg、22 ml/kg)and intra-abdominal pressures(5 mm Hg,10 mm Hg,15 mm Hg).After model was established successsfully,respiratory rates (RR),ABG and death rates were observed at pre-pneumoperitoneum,after 0.5 hour and 2 hours under pneumoperitoneum and 0.5 hour after desufflation.Results Without pneumoperitoneum,different blood loss exerted different effect on arterial blood gas:RR,PaCO_2 increased(P

Sixteen compounds were isolated from the aerial parts of Euphorbia sikkimensis by means of various chromatographic techniques such as silica gel, Sephades LH-20 and RP-18, and their structures were elucidated as naringenin (1), kaempferol (2), quercetin (3), kaempferol-3-O-alpha-L-arabinopyranoside (4), quercetin-3-O-alpha-L-arabinopyranoside (5), quercetin-3-O-(2"-galloyl)-alpha-L-arabinopyranoside (6), 5alpha, 8alpha-epidioxy-(22E, 24R)-ergosta-6,22-dien-3beta-ol (7), stigmast-5-ene-7-one-3beta-ol (8), 3beta-hydroxy4a, 14alpha-dimethyl-5alpha-ergosta-8, 24(28)-dien-7-one(9), beta-sitosterol (10) , 10-cucurbitadienol( 1) , scopoletin(12) , ethyl gallate(13), p-hydroxybenzaldehyde (14), 3 betahydroxybenzeneethanol( 15) ,and 2,4-dihydroxy-6-methoxy-acetophenone (16) on the basis of spectroscopic data analysis. All the compounds are isolated from this plant for the first time, and compounds 1, 4-8, 15 are obtained from Euphorbia species for the first time.
Chromatography ; Euphorbia ; chemistry ; Organic Chemicals ; analysis ; isolation & purification

No abstract available.

Adolescent ; Female ; Humans ; Lung Neoplasms ; congenital ; Lymphoma, Large-Cell, Anaplastic ; physiopathology

Acute Disease ; Adolescent ; Adult ; Aged ; Cyclophosphamide ; administration & dosage ; therapeutic use ; Female ; Hemoperfusion ; Humans ; Male ; Methylprednisolone ; administration & dosage ; therapeutic use ; Middle Aged ; Paraquat ; poisoning ; Renal Dialysis ; Retrospective Studies ; Young Adult

As an exposing part of the human body,facial surface is easy to be injured in our daily lives.With the increase of living standards,the patients have an ever more urgent require for aesthetic treatment for the medical and plastic surgical treatment for the exposing parts of body,especially for the facial surface.But we found in the clinical work some aesthetic treatment were not satisfying,and some patients even needed a second restitution.We have analyzed the reasons for this phenomenon and proposed some corresponding solving methods.

Objective To construct the vector that expresses the fusion protein of HIV-Tat protein and red fluorescent protein(mCherry) in mammalian cells,and observe by fluorescence microscopy the intracellular transduction and localization of recombinant protein in cells,in order to obtain a useful tool for the study of the uptake mechanism and intracellular localization of HIV-TAT.Methods With the designed primer coding mCherry sequence,the mCherry gene was amplified by PCR with the vector pmCherry-C2 as template,and inserted into vector pET14b-His-TAT to construct the expression vector pET14b-His-TAT-mCherry.The constructed vector was then transformed into E.coli BL21(DE3),which had been identified by PCR and double digested with restriction endonuclease,followed by sequencing.After IPTG induction,the recombinant protein of His-TAT-mCherry was lyzed and analyzed with SDS-PAGE.Purified His-TAT-mCherry recombinant protein was added to Hela cells and the fluorescence was observed to evaluate the transduction efficiency.Results The results of identification by PCR,digestion with restriction endonuclease and sequencing indicated that the vector His-TAT-mCherry was correctly constructed.His-TAT-mCherry fusion protein was expressed in mammalian Hela cell line and purified successfully,and the fusion protein showed cellular transduction activity.It was found by fluorescence microscopy that the red fluorescence protein located mainly over the cytoplasm,and also the membrane to some extent.Conclusion The expression vector is successfully constructed for HIV-TAT labeled with mCherry sequence.Effective expression and purification of this fusion protein is achieved.It has been observed that the constructed vector may be expressed in mammalian Hela cell under active condition.Thus,it might be useful in the study of uptake mechanism and intracellular localization of HIV-TAT.

Objective To observe the effect and safety of low dose milrinone used in patients suffering from refractory heart failure and renal dysfunction. Methods Forty-two patients with refractory heart failure and renal dysfunction were divided into treatment group(21 cases ) and control group(21 cases )by random digits table. All the patients accepted a therapy of cardiac booster, diuretics and vasodilators, and treatment group also accepted the therapy of milrinone [0.375 μ g/( kg· min), 10 mg/d, for 7 days]. And then the symptom, signs, blood pressure, heart rate, heart function and renal function before and after the treatment were observed. Results The total effective rate in treatment group was 85.7%( 18/21 ) ,significantly higher than that in control group [57.1% (12/21)] (P ＜0.05＝. After treatment,the heart rate,systolic blood pressure,diastolic blood pressure,stroke volume,cardiac output and left ventricular ejection fraction in treatment group and control group improved significantly than those before treatment, and these index improved better in treatment group [(79.3 ± 12.4) beats/min vs. (85.4 ± 10.2) beats/min, ( 107.6 ± 15.4)mm Hg ( 1 mm Hg = 0.133 kPa) vs.( 119.1 ± 13.5 ) mm Hg, (60.8 ± 9.4) mm Hg vs. (65.8 ± 8.5 ) mm Hg,(66.3 ± 10.2 ) ml vs. (61.2 ± 9.3 ) ml, (5.3 ± 0.6 ) L/min vs. (4.8 ± 0.9) L/min, (56.6 ± 8.4 )% vs. (48.9 ±7.3)% ,P ＜ 0.05＝. In two groups,there were no statistical difference in renal function. Conclusions Low dose of milrinone can improve the heart function of the patients with refractory heart failure and renal dysfunction and has good renal safety.

Objective To study the effect of magnesium citrate combined with compound polyethylene glycol electrolyte powder in bowel preparation before colonoscopy.Methods One hundred and six colonoscopy patients with lower digestive tract symptom were divided into study group (54 cases) and control group (52 cases) by random digits table method,the bowel preparation in study group was magnesium citrate combined with compound polyethylene glycol electrolyte powder,in control group was compound polyethylene glycol electrolyte powder.The lesions detection rate,bowel preparation quality,time of first defecation,number of defecation,time of colonoscopy,results of the electrolyte level and liver and kidney function after medication and adverse reaction were compared between two groups.Results There were no statistical differences in lesions detection rate,time of colonoscopy,results of the electrolyte level and liver and kidney function after medication between two groups (P ＞ 0.05).The total effective rate of bowel preparation quality in study group was significantly higher than that in control group [96.3％(52/54) vs.82.7％ (43/52)],the time of first defecation was significantly shorter than that in control group [(32.5 ± 26.1) rmin vs.(47.2 ± 22.4) min],the number of defecation was significantly much than that in control group [(8.2 ± 2.9) times vs.(6.2 ± 2.6) times],there were statistical differences (P ＜ 0.05).The incidence of nausea in study group was significantly lower than that in control group [22.2％ (12/54) vs.48.1％ (25/52)],there was statistical difference (P ＜ 0.05).There were no statistical differences in the incidences of vomiting,abdominal distention and abdominal pain between two groups (P ＞ 0.05).Conclusion Magnesium citrate combined with compound polyethylene glycol electrolyte powder used in preoperative bowel preparation for colonoscopy has better clinical application value.