1.A Case of Craniofacial Dysostosis(Crouzon's Disease).
Song Tae KIM ; Yong Sang YOO ; Yong Youun CHOI
Journal of the Korean Pediatric Society 1985;28(10):1027-1031
No abstract available.
2.A-V fistula after Palma-Dale crossover venous graft operation in iliac vein occlusion
Sang Seob YOON ; Seung Jin YOO ; Yong Bok KOH
Journal of the Korean Society for Vascular Surgery 1991;7(1):102-107
No abstract available.
Fistula
;
Iliac Vein
;
Transplants
3.Typical Skin Manifestations of Reiter's Syndrome.
Sang Il LEE ; Yong Bum JANG ; Chang Woo YOO ; Wan Hee YOO
The Journal of the Korean Rheumatism Association 2003;10(4):462-463
No abstract available.
Skin Manifestations*
;
Skin*
4.Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application.
Deog Yong LEE ; Young Wook CHO ; Sang Gyun KANG ; Sung Jae SHIN ; Han Sang YOO
Journal of Veterinary Science 2004;5(4):337-343
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6), and combining these with a sensitive and specific capture-ELISA for a diagnostic purpose. cDNA encoding the mature protein coding region of porcine IL-6 was cloned and expressed with pQE-30UA expression vector. rpIL-6 was then expressed and purified by using Ni-NTA resin. Protein mass of 24 kDa was found with SDS-PAGE and the identity of the protein was confirmed by Western-blot. Production of polyclonal antibodies against rpIL-6 was performed using the purified rpIL-6 in mice and hens. An antigen capture-ELISA was developed with the antibodies after their extraction. To compare the IL-6 level in the different sanitary state of farms, pig sera were randomly collected and concentration of IL-6 in the sera was measured with the antigen capture-ELISA. The capture-ELISA with the optimal concentration of antibodies, in this study, was able to detect about 10 ng/ml of rpIL-6. IL-6 levels determined with the capture-ELISA in pig sera showed positive correlation with the sanitary states of the farms. These results suggested that the developed antigen capture-ELISA could be a good tool for the screening of microbial infection in pig farms.
Animals
;
Biological Markers/blood
;
Blotting, Western/veterinary
;
Chickens
;
Cloning, Molecular
;
DNA, Complementary/genetics/isolation&purification
;
Electrophoresis, Polyacrylamide Gel/veterinary
;
Enzyme-Linked Immunosorbent Assay/methods/*veterinary
;
Female
;
Immunoglobulins/*blood
;
Interleukin-6/*immunology
;
Mice
;
Mice, Inbred ICR
;
Recombinant Proteins/immunology
;
Swine/*immunology
5.The Risk Factors Associated with Hip Dislocation after Total Hip Replacement.
Won Yong SON ; Joon Kyu MOON ; Sang Wwhan HAN ; Jea Hyuk YANG ; Soon Yong YOO
Journal of the Korean Hip Society 2006;18(4):167-172
Purpose: Dislocation is the second most common cause of failure, after implant loosening, in revisional THA (Total hip arthroplasty), and its evaluation and treatment still remain controversial issue. This study was undertaken to evaluate the risk factors after THA using the posterolateral approach and posterior soft tissue repair. Materials and Methods: Between January 1998 and May 2003, 211 consecutive primary total hip replacement arthroplasties using the posterolateral approach and posterior soft tissue repair were performed by the same surgeon. To compare the dislocation groups (6 cases) with the non-dislocation groups (205 cases), we randomized 120 of the non-dislocation cases. The risk factors for hip dislocation were categorized into patient factors and surgical factors. Patient factors included gender, age, preoperative diagnosis, underlying systemic disease, and alcoholic history. Surgical factors included position of the component (acetabular version and inclination, femoral anteversion), leg length discrepancy, and sum of anteversions of the cup and stem. Their parameters were measured on postoperative radiographs. Statistics were performed with Fishe`s Exact test and T test. Results: Dislocations occurred at an overall incidence rate of 2.8% (6/211cases). There were 4 (1.9%) cases of anterior dislocations and 2 (0.9%) cases of posterior dislocations. Dislocations occurred on average at postoperative day 6.1 and all dislocated hips were reduced with the closed method, except for one case that was treated surgically. There was no statistical significance in patient factors between the two groups, except for neurologic disease and alcoholic history. However, the sum of the acetabular and femoral anteversions in the anterior dislocation group was larger than that of the non-dislocation group by approximately 19 degrees. Conclusion: Our results demonstrated that by using the posterior approach and repair of soft tissue, the posterior dislocation rate after total hip replacement arthroplasty can be reduced and the sum of the acetabular and femoral anteversions had more influence on dislocations after THAs than did either anteversion alone.
Acetabulum
;
Alcoholics
;
Arthroplasty
;
Arthroplasty, Replacement, Hip*
;
Diagnosis
;
Dislocations
;
Hip Dislocation*
;
Hip*
;
Humans
;
Incidence
;
Leg
;
Risk Factors*
6.Closed Intramedullary Nailing of Diaphyseal Forearm Fracture in Adolescence
Yung Khee CHUNG ; Jung Han YOO ; Baek Yong SONG ; Yong Wook PARK ; Sang Cheol BAIK
The Journal of the Korean Orthopaedic Association 1995;30(5):1416-1422
Fractures of the shaft of the radius and ulna occur commonly in children and are usually treated by closed reduction and plaster cast immobilization. Anatomic reduction is seldom necessary because of the remodelling potential in the child under 10 years of age, whereas the bones of children older than 10 years of age have less capacity to remodel and the diaphyseal fracture is unstable. In case of either unacceptable reduction or unstable fractures in adolescent patients, an operative treatment is required. In five children older than 12 years of age for whom conservative treatment had hailed, we treated a closed intramedullary nailing using a distal radial and proximal ulnar approach, and followed up for 1 year or more. All fractures healed within 6 weeks. No nonunion, cross-union or refrature occured. Another advantages of this method are negliable cosmestic defect and easy removal of the internal fixation device under local anesthesia. We think that closed intramedullary nailing with rush pin is a safe and reliable method to treat unstable forearm fracture in children older than 12 years of age.
Adolescent
;
Anesthesia, Local
;
Casts, Surgical
;
Child
;
Forearm
;
Fracture Fixation, Intramedullary
;
Humans
;
Immobilization
;
Internal Fixators
;
Methods
;
Radius
;
Ulna
7.Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR.
Yong Hwan KIM ; Kyu Woan CHO ; Hwa Young YOUN ; Han Sang YOO ; Hong Ryul HAN
Journal of Veterinary Science 2001;2(1):59-63
A one step reverse transcription PCR (RT-PCR) combined nested PCR was set up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement of nested PCR. Two PCR primer sets were designed based on the sequence of nucleocapsid gene of CDV Onderstepoort strain. One-step RT-PCR with the outer primer pair was revealed to detect 10(2) PFU/ml. The sensitivity was increased hundredfold using the one-step RT-PCR combined with the nested PCR. Specificity of the PCR was also confirmed using other related canine virus and peripheral blood mononuclear cells (PBMC) and body secretes of healthy dogs. Of the 51 blood samples from dogs clinically suspected of CD, 45 samples were revealed as positive by one-step RT-PCR combined with nested PCR. However, only 15 samples were identified as positive with a single one step RT-PCR. Therefore approximately 60% increase in the efficiency of the diagnosis was observed by the combined method. These results suggested that one step RT-PCR combined with nested PCR could be a sensitive, specific, and practical method for diagnosis of CDV infection.
Animals
;
Distemper Virus, Canine/genetics/*isolation & purification
;
Dogs
;
Polymerase Chain Reaction/*methods/*veterinary
;
RNA, Viral/genetics/isolation & purification
;
Reverse Transcriptase Polymerase Chain Reaction
;
Vaccines, Attenuated
;
Viral Vaccines
8.Efficacy and Safety of Nifedipine Gastrointestinal Therapeutic System(Adalat OROS) in Patients with Mild to Moderate Essential Hypertension.
Dae Hyun KIM ; Se Ick OH ; Yong Kyun KIM ; Suck Koo CHOI ; Won Sang YOO
Korean Circulation Journal 1992;22(3):488-493
BACKGROUND: This study was designed to evaluate the antihypertensive efficacy and safety of nifedipine gastrointestinal system push-pull osmotic pump formulation in patients with mild to moderate essential hypertension. METHODS: After 2 weeks placebo run-in period, nifedipine 60 mg was administered once daily for 8 weeks in thirty-two patients with mild to moderate essential hypertension. RESULTS: At the end point of treatment, sitting blood pressure decreased as much as 33/16mmHg in average and rewarded 88% of efficacy and 69% of normalization. The ambulatoy blood pressure monitoring in 10 patients also revealed 11% decrease in 24 hr mean diastolic blood pressure and 32% decrease in % elevated blood pressure. The most frequent side reactions were constipation in 7 patients and polyuria in 6 patients which were tolerated during entire trial period. There were no significant changes in biochemical parameters and hematologic data, thus making the rate of safety 91% and overall rating of usefulness was 84%. CONCLUSION: Nifedipine GITS 60 mg once daily regimen is well tolerated and effective in the treatment of mild to moderate essential hypertension.
Blood Pressure
;
Blood Pressure Monitors
;
Constipation
;
Humans
;
Hypertension*
;
Nifedipine*
;
Polyuria
;
Reward
9.A Case of Fatal Intracranial Hemmrhage due to Vitamin K Deficiency.
Yong Sang YOO ; Moon Tae JEONG ; Jai Sook MA ; Tai Ju HWANG
Journal of the Korean Pediatric Society 1986;29(1):108-112
No abstract available.
Vitamin K Deficiency*
;
Vitamin K*
;
Vitamins*
10.Binding of fibronectin to staphylococcus aureus.
Jung Wan KIM ; Sang Hwa LEE ; Yoo Chul LEE ; Sung Yong SEOL ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1993;28(6):431-441
No abstract available.
Fibronectins*
;
Staphylococcus aureus*
;
Staphylococcus*