Objective:To observe the clinical effects of collateral-pricking and cupping therapy plus moxibustion for localized neurodermatitis.
Methods:A total of 94 cases with localized neurodermatitis were randomly divided into two groups by their visit order, 47 cases in each group. The cases in the treatment group were treated by bleeding method with a plum blossom needle, cupping method and moxibustion. The cases in the control group were treated by subcutaneous injection of Triamcinolone Acetonide Acetate Injection.
Results:After treatment, the curative rate and total effective rate were respectively 80.9% and 100% in the treatment group and were 48.9% and 87.2% in the control group. The differences in the curative rate and total effective rate between the two groups were statistically significant (P<0.01,P<0.05).
Conclusion:Bleeding method by a plum blossom needle, in combination with cupping method and moxibustion, is an effective therapy for localized neurodermatitis.

Objective: To investigate fetal rats abnormal electrocardiograms in intrahepatic cholestasis of pregnancy(ICP).Methods: The animal model of intrahepatic cholestasis of pregnancy was induced by 17-?-Ethinylestradiol and Progesterone.The electrocardiograms of fetal rats by conceptual day 20th(Group1),day 21st(Group2) were evaluated with Powerlab biologic signal extracting and analyzing system Chart 5 Software.Results: ①The serum total bile acids(TBA) levels of pregnant rats of ICP were 64.3?5.2(?mol/L) in group 1,78.5?4.5(?mol/L) in group 2,respectively,which showed significant differences(P

Objective:To investigate the effect to fetal growth in intrahepatic cholestasis of pregnancy(ICP).Method:The fetal birthweight of 332 cases ICP from January 1999 to December 2003 were analyzed carefully.Results:(1) In all 332 cases of ICP,the occurrence of preterm was 29.2% which was significantly higher than the normal control group,and the occurrence of Small for Gestational Age(SGA)was 5.1% which was no significant difference when compared to the normal control group.(2) There were 3.4%,76.5% and 20.7% cases of SGA,AGA and LGA in mild ICP group,respectively.No significant differences were found when compared to the constituent ratio of SGA,AGA and LGA between the mild and severe ICP group(3)There were no significant differences about the odds ratio of SGA,AGA and LGA in different courses of ICP.Conclusion:It demonstrated that no chronic placental dysfunction and no obvious effect to fetal growth in ICP.

Objective To investigate the effect of Interleukin(IL)-18,IL-12 and tumor necrosis factor-α(TNF-α)in hepatic injury in intrahepatic cholestasis of pregnancy(ICP).Methods Sixty-two cases of ICP patients(ICP group),30 cases of normal pregnant women(control group)and 30 cases of hepatitis B(HBV) women (hepatitis group) were recruited. Serum IL-18, IL-12 and TNF-α were examined by ELISA. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were examined by automatic biochemical analysis instrument. Results ( 1 ) In hepatitis group, serum concentrations of IL-18,IL-12 and TNF-α were (256±51 ) ng/L, ( 122±96) ng/L and (207±3) ng/L; serum levels of ALT and AST were(363±174) U/L and (359 ±237) U/L, respectively. In ICP group, serum concentrations of IL18, IL-12 and TNF-α were (72±32) ng/L, (42 ±28) ng/L and (48±14) ng/L; serum levels of ALT and AST were (201 ±128) U/L and ( 132±87) U/L, respectively. While in control group, serum concentrations of IL-18, IL-12 and TNF-α were (43 ± 13) ng/L, ( 10±3) ng/L and (33±9) ng/L; serum levels of ALT and AST were (13 ~ 4) U/L and (15 ± 3) U/L, respectively. Serum IL-18, IL-12, TNF-α, ALT and AST levels in hepatitis group were significantly higher than those in ICP group and control group ( P ＜0. 05 ).Serum IL-18, IL-12, TNF-α, ALT and AST levels in ICP group were significantly higher than those in control group(P ＜ 0. 05 ). (2) In severe ICP subgroup, serum concentrations of IL-18, IL-12 and TNF-α were (81 ±32) ng/L, (50 ±25) ng/L and(50 ± 14) ng/L; serum levels of ALT and AST were (269 ± 111 ) U/L and (181±73) U/L In mild ICP subgroup, serum concentrations of IL-18, IL-12 and TNF-α were (48 ±18 ) ng/L, (17 ± 4 ) ng/L and (40 ± 10 ) ng/L; serum levels of ALT and AST were (87±46) U/L and (50 ±21 ) U/L, respectively. Serum IL-18, IL-12, TNF-α, ALT and AST levels in severe ICP subgroup were significantly higher than those in mild ICP subgroup and control group (P ＜ 0. 05). And serum ALT and AST levels in mild ICP subgroup were significantly higher than those in control group(P ＜0. 05). (3) There were 16 cases with preterm birth (50%, 16/32 ) and 10 cases with meconium-stained amniotic fluid( 31%, 10/32 ) in severe ICP subgroup, significantly higher than those in mild ICP subgroup ( P＜ 0. 05 ), which contained 2 preterm births ( 7%, 2/30) and 1 meconium-stained amniotic fluid (3%, 1/30). While in control group, the numbers were 1(3%, 1/30)and 1(3%, 1/30),respectively. As for the cases of neonates whose 1 minute Apgar score were not more than 7, there were 2 cases, 1 case and 1 case in severe ICP subgroup, mild ICP subgroup and control group, respectively,which showed no significant difference(P＞ 0. 05). Conclusion Serum IL-18, IL-12 and TNF-α may be involved in the process of hepatic injury of ICP.

Objective To explore transthyretin (TTR) effect on retinal vascular endothelial cells (hREC) under high glucose and hypoxia environment.Methods hREC and human retinal pigment epithelial cell (hRPEC) were cultured at low-glucose (LG),high glucose (HG) and hypoxia.The glucose concentration was increased from 5.5 mmol/L up to 25 mmol/L,and hypoxia was induced by 200 μmol/L CoCl2.The cells were divided into LG group,LG-hypoxia group,HG group,HG-hypoxia group according to the different cell culture environment.The growth index was detected at 0,4,8,16,24,36,48,60,72 hours after cultured.Furthermore,hREC and hRPEC were also cultured with additional TTR (4 μmol/L),respectively.Then transwell co-culture system was employed to reveal the effects of hRPEC on the growth of hREC.Results At 72 hours after cultured,the growth index of hREC and hRPEC in LG group were increased as compared with LG-hypoxia group and HG group (hREC:F=17.098,22.970;P＜0.05.hRPEC:F=45.442,9.011;P＜0.05);the growth index of hREC and hRPEC were decreased in HG group and HG-hypoxia group (hREC:F=146.184,P＜0.05;hRPEC:F=27.907,P＜0.05).Additionally,hREC could be significantly repressed by added TTR during culture with high concentration of glucose (F=161.430,24.106;P＜0.05).hREC could be significantly increased by added TTR during culture with low concentration of glucose (F =200.486,48.662;P ＜ 0.05).In co-culture process,hRPEC revealed inhibition activity against hREC under both natural and abnormal environment (LG group:F=15.711,P＜ 0.05;LG-hypoxia group:F =45.659,P＜0.05;HG group:F =7.857,P ＜0.05;HG-hypoxia group:F=6.348,P＜0.05).Conclusion Under high glucose and hypoxia environment,the growth of hREC from neovascular could be inhibited by TTR.

AIM: To observe and compare efficacy of the lower eyelid twitch shift joint outer canthal ligament shortening surgery and orbicularis muscle resection surgery to treat degenerative entropion.
METHODS: Patients with degenerative entropion in our hospital were selected. The test group was 40 cases ( 70 eyes), of which double eyes with attack (30 cases), and lower eyelid twitch shift joint outer canthal ligament shortening surgery was applied to the test group. Control group was 20 cases (26 eyes), of which double eyes with attack ( 6 cases ) , and the control group was used by orbicularis muscle resection surgery. The correction rate, double eyelid symmetry and overcorrection rate were observed in two groups at postoperative 1wk. The long-term recurrence rate, double eyelid symmetry and overcorrection rate with follow-up 6mo were observed.
RESULTS: After 1wk, the correction rate of experimental group 98. 6%, undercorrection rate of that was 1. 4%, all the eyelid was symmetry, only one eye with a slight overcorrection. Correction rate of control group was 92. 3%; all the eyelid was symmetry, and the poor rate of this group was 7. 7%. After 6mo, correction rate of experimental group was 95. 2%; undercorrection rate of experimental group was 3. 2%, and overcorrection rate was 1. 6%. Correction rate of control group was 87%, and 2 eyes of recurrence, 1 eye with a poor overcorrection. Double eyelid was symmetry, overcorrection rate difference was not statistically significant ( P > 0. 05 ), and the correction rate were significantly different (P<0. 05).
CONCLUSION: Compared toorbicularis muscle resection surgery, postoperative recurrence rate of lower eyelid twitch shift joint outer canthal ligament shortening surgery is significantly lowered.

Consumer Product Safety ; legislation & jurisprudence ; Legislation, Food ; Toxicology

Objective The genetic diversity of the natural populations of Gardenia jasminoides were investigated to provide scientific basis for its resources protection and rational utilization. Methods Fourteen pairs of EST-SSR primers were screened in 19 natural populations of 573 individuals to calculate the genetic parameters of G. jasminoides, and further cluster analysis was then carried out. Results Fourteen pairs of EST-SSR primers generated 75 loci, which showed high genetic diversity maintained in natural populations of G. jasminoides (He = 0.703). Mean population gene diversity (Nei) within populations was 0.603, the Shannon’s diversity index (I) was 1.10. Moderate genetic differentiation (Fst= 0.141) and high gene flow (Nm = 1.523) among populations have been showed too. AMOVA analysis revealed that genetic variation within populations was the main sources of total variation. The Mantle test showed there was no significant correlation between genetic distances and geographic distances. Moreover, significant bottlenecks effects in two-phased model of mutation (TPM) test in 73.7% populations were detected in recent history. Conclusion The results in this study indicated that high level genetic diversity were existed in the natural G. jasminoides populations.

Objective: In order to obtain SSR primers which has good universality among the main tree species of Clerodendrum L., and then molecular screening of DNA fingerprints was used in closely related species and genetic research of these ethnical medicinal resources. Methods: An experiment was conducted to study the transferability of 19 pairs of SSR primers developed from C. izuinsulare and C. trichotomum and amplified in nine samples from nine species of Clerodendrum L. Results: Seventeen pairs of 19 primers had the amplification products in C. cyrtophyllum, a cosmopolitan species, the transferability ratio was the highest (89.5%). Seven pairs of primers were successfully amplified in all samples of Clerodendrum L. species, and the transferability ratio was 36.8%. Six pairs of polymorphism SSR primers were used to construct the DNA fingerprints of nine Clerodendrum L. species. In the primers, the CI140 identified seven Clerodendrum L. species except C. fortunatum and C. lindleyi. Coupled with CI132, CT042, CI107, or CI143, they identified all of the nine Clerodendrum L. species. Then, the genetic distances were used to generate a UPGMA tree. The results showed that C. fortunatum, belonging to Ser. Axilliflorae schauer, formed a cluster, and C. mandarinorum and C. cyrtophyllum formed another cluster, while the clusters of the others were difference from the shape classifications. Conclusion: The experiment proves a good way to develop Clerodendrum L. SSR primers from other related species. This study provides the important reference for the use of molecular markers of Clerodendrum L.

Calcineurin dephosphorylates multiple serine residues near the N terminus of NFAT proteins enabling them to translocate from cytoplasm to nucleus, where they activate a subset of hypertrophic response genes. Transgenic mice over-expressing a constitutively active form of calcineurin or NFAT3, developed obviously hypertrophy and heart failure or sudden death proving its pathogenic role. Here we used literatures on MEDLINE (2000-2011), systematically reviewed the new development of calcineurin signaling pathway in myocardial hypertrophy.