1.Effects of Alcohol Extract of White-flower Hogfennel(Peucedanum praeruptorum) on Left Ventricular Hypertrophied Hypertensive Rats
Chinese Traditional and Herbal Drugs 1994;0(07):-
In left ventricular hypertrophied hypertensive rats (LVH), effects of alcohol extract of Peucedanum praeruptorum(QHJG)on left ventricular(LV)compliance and Ca2+content in left ventricular myocardial mito chondria and tail artery were studied.QHJG 0.6g/100g(body weight).d was given for 9 weeks (ig). The LV compliance in LVH group was significantly decreased as compared with that in the sham-operated group. The Ca2+ content in left ventricular myocardial mitochondria and tail artery of LVH group was markedly raised. But QHJG could mardedly im prove the LV compliance and decrease the Ca2+ of left ventricular myocardial mitochondria and tissues of tail artery from LVH rats.These results suggest that QHJG can improve the LV compliance in LVH rats,which maybe related to its reduction of Ca2+ content of LV myocardial mitochondria and tissues of tail artery
2.Detection of high-risk human papillomavirus types-16 and-18 in laryngeal squamous cell carcinoma
Peng WANG ; Xiaosong RAO ; Yong LI ; Tao NING ; Baoguo LIU
Cancer Research and Clinic 2011;23(1):14-17
Objective To investigate the relationship between human papilloma virus(HPV)infection and the development of laryngeal squamous cell carcinoma(LSCC).Methods To elucidate the role of HPV in the development of LSCC,we employed polymerase chain reaction(PCR)based on four pairs of primers an4 in situ hybridization(ISH)to screen the HPV infection in 84 ISCC tissues.Results Using HPV L1 general primer amplification,HPV DNA was detected in 23(27.4%)of the 84 LSCC samples.However,when specific primers for HPV-16 or-18 were used to amplify E6 and E7 in all samples,29 cases(34.5%)were positive for HPV-16,while 6 cases(7.1%)were positive for HPV 18.Coinfeetion of HPV-16 and-18 were found in 4cases (4.8%).Overall,HPV type 16 and 18 infections were present in 36.9% of the LSCC samples.In addition,the positive rate of HPV 16 E6 mRNA was 30.9%(26/84)in LSCC by ISH with digoxin-labeled sense probes of HPV 16 E6.Conclusion High-risk HPV-16may be an etiologic factor in the development of laryngeal squamous cell carcinoma, while the complicated molecular mechanism of HPV16 inducedtumorgenesis needs a further study.
3. Preparation and in vivo and in vitro evaluation of Pulsatilla Saponin D Solid Dispersions
Chinese Traditional and Herbal Drugs 2015;46(21):3179-3184
Objective: To prepare the solid dispersion of Pulsatilla saponin D (PSD-SD) and evalution its in vivo and in vitro drug release behavior. Methods: The PSD-SD was prepared by solvent method. Three carriers were used in the PSD-SD. Infrared spectroscopy (IR), differential thermal analysis (DSC), and X-ray diffraction (XRD) were used to determine the PSD-SD. Dissolution rates and pharmacokinetic parameters were evaluated in vitro and in vivo characteristics of the PSD-SD. Results: When the PEG 6000 was used as carrier, the solubility of PSD was increased from 2.39 to 7.06 mg/mL, and the cumulative release rate of PSD reached 90% in 60 min, and the bioavailability of PSD was increased to 2.24 times. Conclusion: The solid dispersion prepared PSD can increase the solubility, dissolution rate, and bioavailability.
4. Study on in situ intestinal absorption of Pulsatilla saponin D in rats
Chinese Traditional and Herbal Drugs 2013;44(24):3515-3520
Objective: To investigate the intestinal absorption characteristics of Pulsatilla saponin D in rats. Methods: In situ single-pass intestinal perfusion model was used to inspect the absorption of Pulsatilla saponin D in the intestinal tract of rats. HPLC was used to determine the concentration of Pulsatilla saponin D in intestinal perfusion fluid samples. Absorption rate constant (Ka) and apparent permeability coefficient (Papp) were used as indexes to investigate the effects of absorption sites, drug concentration, different pH values, and P-glycoprotein (P-gp) inhibitor on Pulsatilla saponin D absorption. Results: There was the significant difference (P<0.05) of intestinal perfusion fluid samples in each intestinal segment and the order of the Ka and Papp values of Pulsatilla saponin D in each intestinal segment was colon>ileum>jejunum>duodenum. With the pH value increasing, the Ka and Papp values also increased and both of them had significant differences (P<0.05). The colon absorption of perfusion fluid at different concentration (0.30, 0.15, and 0.08 mg/mL) had no significant difference (P>0.05); There was significant difference (P<0.05) in Ka and Papp values with and without P-gp inhibitor. Conclusion: Pulsatilla saponin D could be well absorbed in whole intestinal segments of rats, and the best intestinal absorption site is colon; The drug concentration in a certain range has no effect on Ka and Papp values, which preliminarily comfirms that the obsorption mechanism of Pulsatilla saponin D could be passive diffusion; Pulsatilla saponin D may be a substrate of P-gp and possess the saturation phenomenon of transporters.
5.Radiosensitization of microRNA-17-92 on human mantle cell lymphoma cells in vitro
Ping JIANG ; Junjie WANG ; Yong ZHAO ; Enyu RAO ; Na MENG ; Zhenyou YOU
Chinese Journal of Radiological Medicine and Protection 2010;30(4):403-406
Objective To investigate the effects of microRNA-17-92 on radiosensitivity of human mantle cell lymphoma cells. Methods Tetracycline-regulated pRevTet-On expression system was established to generate cell line Z138c-miR-17-92 with over-expressed miR-17-92 and cell line Z138c-TMP2. Cell proliferation was measured by 3 H-TdR incorporation and viable cell counting stained with typan blue. Cell cycle distribution was analysed by flow cytometry(FCM). Results More viable and proliferous cells were counted in group miR-17-92,when exposure dose was greater than 2 Gy and incubation time was longer than 48 h under the same condition (t = -3. 12 and -3.28,P <0. 05). The percentage of G2/M cells in group TMP2 was increased while no obvious cell cycle arrests were found in group miR-17-92 at 2 and 4 Gy (t = 2. 885, P < 0.05 ). When cells were incubated for 96 h, higher percentage of propidium iodide (PI) positively stained cells were found in group TMP2 (24. 02% vs. 36. 16% )compared with group miR-17-92 (6.49% vs. 11.39% ) at 2 and 4 Gy, respectively( t = - 17.59, - 4. 972, P < 0.05 ).Conclusions Overexpression of microRNA-17-92 decreased the radiosensitivity of human mantle cell lymphoma cells by inhibition of cell cycle changes and cell apoptosis.
6.Isolation,Culture,Identification and Induced Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord
Na XIE ; Ming LOU ; Guozhou RAO ; Yong ZHU ; Chengfang TANG ; Lin WANG
Journal of Modern Laboratory Medicine 2015;(3):67-70
Objective To establish the method of isolation,culture and identify biological characterization of mesenchymal stem cells from human umbilical cord (hUCMSCs);and study their multiple differentiation potency.Methods Stem cells from human umbilical cord were cultured by enzyme Wharton jelly method in vitro.The surface markers were identified by flow cytometry.Multi-differentiation capacity was identified by osteogenic and adipogenic differentiation.ALP was detected with Calcium cobalt staining.The mineralized ability in vitro was measured with Alizarin red staining.Theadipocyte differen-tiation ability was measured with oil red-O staining.Results Flow cytometry analysis revealed that CD73 (92.45%),CD90 (95.45%)and CD105 (96.45%)were highly expressed on these cells’surface,while CD34 (1.07%)were negative ex-pressed.Cells were cultured with induced-osteogenic medium after 3 weeks,ALP staining in the cytoplasm of black parti-cles,and a large amount of mineralized nodules within cells was observed after 4 weeks.Cells were cultured with induced-adi-pogenic medium after 2 weeks,the majority of these cells were round,oil red O staining of lipid droplets generated within cells was observed.Conclusion Mesenchymal stem cells from human umbilical cord have the potential of multi-directional differentiation.These cells could be induced to differentiate into adipocytes and osteoblasts,which laid the foundation for clinical stem cell therapy research source of seed cells.
7.Effect of air humidity on traditional Chinese medicine extract of spray drying process and prediction of its powder stability.
Yan HE ; Yin XIE ; Long-jin ZHENG ; Wei LIU ; Xiao-yong RAO ; Xiao-jian LUO
China Journal of Chinese Materia Medica 2015;40(3):424-429
In order to solve the adhesion and the softening problems of traditional Chinese medicine extract during spray drying, a new method of adding dehumidified air into spray drying process was proposed, and the storage stability conditions of extract powder could be predicted. Kouyanqing extract was taken as model drug to investigate on the wet air (RH = 70%) and dry air conditions of spray drying. Under the dry air condition, the influence of the spray drying result with different air compression ratio and the spray-dried powder properties (extract powder recovery rate, adhesion percentage, water content, angle of repose, compression ratio, particle size and distribution) with 100, 110, 120, 130, 140 °C inlet temperature were studied. The hygroscopic investigation and Tg value with different moisture content of ideal powder were determined. The water activity-equilibrium moisture content (aw-EMC) and the equilibrium moisture content-Tg (EMC-Tg) relationships were fitted by GAB equation and Gordon-Taylor model respectively, and the state diagram of kouyanqing powder was obtained to guide the rational storage conditions. The study found that in the condition of dry air, the extract powder water content decreased with the increase of air compression ratio and the spray drying effect with air compression ratio of 100% was the best performance; in the condition of wet air, the extract powder with high water content and low yield, and the value were 4.26% and 16.73 °C, while, in the dry air condition the values were 2.43% and 24.86 °C with the same other instru- ment parameters. From the analysis of kouyanqing powder state diagram, in order to keep the stability, the critical water content of 3.42% and the critical water content of 0.188. As the water decreased Tg value of extract powder is the major problem of causing adhesion and softening during spray drying, it is meaningful to aid dehumidified air during the process.
Drug Stability
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Drugs, Chinese Herbal
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chemistry
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Humidity
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Medicine, Chinese Traditional
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Plant Extracts
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chemistry
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Powders
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Temperature
8.Genetic Transformation of Candida glycerinogenes by REMI and Electroporation
Yong-Guang ZHANG ; Wei SHEN ; Zhi-Ming RAO ; Hui-Ying FANG ; Jian ZHUGE ;
Microbiology 1992;0(05):-
In order to isolate genes related with the osmoadaptation and glycerol metabolism of Candida glycerinogenes, a transformation system based on the dominant selectable marker Zeocin and restriction enzyme-mediated integration (REMI) was established. Effects of seven restriction enzymes on transformation efficiency of C.glycerinogenes were tested. Transformation conditions were optimized in the presence of Hind III. Under the optimal conditions of OD_ 600 ≈1.3, voltage of 1.5 kV, 2.0?10~9 competent cells/mL, 100 units of Hind III added, the transformation efficiency was up to 129 trnaformants/?g DNA. 58% of transformants were stable on nonselective medium. These results suggest that REMI technique would be beneficial to the genetic transformation of C.glycerinogenes.
9.Association of genetic polymorphisms of STAT4, the cytotoxic T-lymphocyte antigen-4 and chromosome 9p21.3 with susceptibility to rheumatoid arthritis in Minnan population
Chenna ZHENG ; Huachun RAO ; Cailin LUO ; Qingyao WANG ; Huiyong YANG ; Yong DIAO
Chinese Journal of Rheumatology 2016;20(6):372-377
Objective A very high prevalence of rheumatoid arthritis (RA) is observed in Minnan population in China.We aimed to explore the genetic characteristics of RA in Minnan population and genetic mechanisms of RA by studying the associations of three single nucleotide polymorphisms (SNPs) of signal transducer and activator of transcription 4 (STAT-4) (rs7574865),the cytotoxic T-lymphocyte antigen-4 (CTLA)-4 (rs3087243) and chromosome 9p21.3(rs1333049) with RA in Minnan population.Methods A case-control study of 119 RA patients and 125 normal controls from Quanzhou were enrolled.SNPs (rs7574865,rs3087243,rs1333049) were genotyped by allele-specific polymerase chain reaction (PCR) and analyzed by SPSS 18.0.x2-test was applied to compare allele and genotype frequeneies betweeen cases and controlsLogistic regression models were used to analyze the SNPs.Results The results showed the genotype distributions of STAT4 genes were significantly different between case and control groups (P<0.01).Compared with the GT heterozygous genotype,TT and GG homozygosity carriers had a lower risk (OR=0498 and 0.300,P=0.018 and P=0.002 respectively).There was not statistical difference in genotypes and allele in CTLA-4 (rs3087243) between RA patients and healthy controls (x2=4.083,P=0.130),but compared with the AG genotyoe,GG homozygosity carriers had a lower risk on basis of statistics (OR=0.580,P=0.04).There was not statistical difference in genotypes and allele in the chromosome 9p21.3 (rs1333049) (P>0.05),but compared with the GG genotype carriers,CC and GC genotypes carriers had a lower risk on basis of statistics (OR=0.565,P=0.0495).Conclusion Chromosome 9p21.3 (rs1333049) and CTLA-4 rs3087243 G/A may not be associated with susceptibility to RA in Minnan popula-tions.This replication study confirmes that STAT4 rs7574865 G/T polymorphism is associated with susceptibility to RA in Minnan population.
10.EARLY EFFECT OF MEDIUM-DOSE IONIC IRRADIATION (20Gy)ONTHE EXPRESSION OF c-fos IN THE RAT BRAIN
Yong QIU ; Mei SHI ; Chaoyang SUN ; Lichun WEI ; Li DUAN ; Liangwei CHEN ; Zhire RAO
Acta Anatomica Sinica 1957;0(04):-
Objective To investigate the early effect of medium-dose ionic irradiation on the expression of Fos protein in the rat brain. Methods Fos protein was observed in rat brains at times ranging from 24 hours to 4 weeks after hemispheric irradiation (single-fraction maximal dose of 20Gy) with the immunohistochemical technique. Results Compared with that of the un-radiated rats,the expression of Fos protein in the irradiated brain decreased distinctly 24 hours and 1 week after irradiation.However,the quantity of Fos immunopositive cells increased gradually afterwards.At four weeks after radiation,expression of Fos protein recovered progressively in medulla oblongata and pons,in which Fos immunopositive cells were more than those in control group.In contrast,expression level of Fos protein in mesencephalon,diencephalons or telencephalon was still less compared with that of the un-irradiated rats.Conclusion The result suggested that the neuronal activity might be inhibited in certain nuclei of the rat brain in early stages after hemisphere irradiation,and this inhibitory phenomenon was more obviously in higher neural centers.