2.Histopathologicai findings of Cyclosporine A induced hepatotoxicity and nephrotoxicity in laryngeal allograft recipients
Yong QING ; Jibao WANG ; Jinzhi CHEN ;
Chinese Journal of Organ Transplantation 1996;0(04):-
By establishing experimental modal of laryngeal allograft,the short-term histopathological changes of liver and kidney in Cyclosporine A (CsA)-treated rats receiving laryn- geal allograft were observed.The animals were divided into 3 groups.Group 1 was given CsA 15 mg?kg~(-1)/d by daily intraperitoneal injection for 2 weeks,Group 2 received CsA 25 mg?kg~(-1)/d, and the third group without CsA treatment served as control.All of recipients were sacrificed 14 days after transplantation.Histological examination showed that CsA nephrotoxicity was charac- terized by abundant vacuolation of the proximal tubular epithelium cells,hyaline regeneration of arterioles with thickening of vascular wall,and striped interstitial fibrosis and its hepatotoxicity by fatty degeneration with mild hyperplasia of Kupffer's cells and focal necrosis of hepatocytes. Histopathological changes of CsA-induced hepato- and nephrotoxicity of the recipients were closely correlated to the dosage of CsA received.
5.Construction the eukaryotic expression vector of human decay accelerating factor and transfection NIH/3T3 cells
Qing QIAO ; Yong CHEN ; Kefeng DOU ; Jing ZHANG ; Jianpin LI
Journal of Medical Postgraduates 2003;0(04):-
Objective: To construct eukaryotic expression vector pSecTag2/HygroB-DAF of human decay accelerating factor (DAF) and transfect NIH/3T3 cells after encapsulated by chitosan. Methods:The human DAF fragments were obtained by PCR form DAF-pGEM-T Easy Vector, cloned into the eukaryotic expression vector pSecTag2/HygroB, and identified by restriction endonuclease’s digestion and DNA sequencing. After the particles of pSecTag2/HygroB-DAF were encapsulated by chitosan, the NIH/3T3 cells were transfected by chitosan-DAF nanoparticles and detected DAF expression by immunohistochemistry stain. Results:The DAF fragment was 1049 bp. Its sequence was as same as DAF cDNA in Genebank. After having been transfected by chitosan-DAF nanoparticles 24 hours, the NIH/3T3 cells showed diffusely positive in cytoplasms by anti-DAF immunohistochemistry. Conclusion:Eukaryotic expression vector of human DAF were constructed successfully and transfected it to NIH/3T3 cells after encapsulated by chitosan.
8.Anaphylactic shock in a boy after taking oral ofloxacin.
Yan-ming GU ; Xiao-yi CHEN ; Yong-qing WANG
Chinese Journal of Pediatrics 2005;43(8):607-607
Administration, Oral
;
Anaphylaxis
;
chemically induced
;
physiopathology
;
therapy
;
Anti-Bacterial Agents
;
administration & dosage
;
adverse effects
;
Blood Pressure
;
drug effects
;
Child
;
Diarrhea
;
drug therapy
;
Humans
;
Male
;
Ofloxacin
;
administration & dosage
;
adverse effects
;
Treatment Outcome
9.Significance of fiberoptic bronchoscopic brush cytology in lung carcinoma diagnosis
Yong CHEN ; Yanhua CHANG ; Qing GUO ; Ying CAI ; Rongchao SUN
Clinical Medicine of China 2015;31(8):737-739
Objective To evaluate the significance of fiberoptic bronchoscopic brush cytology in the diagnosis and histological classification of lung carcinoma.Methods Data of 309 patients with lung carcinoma were retrospectively analyzed.Both bronchoscopic cytology and histology diagnosis were available.The positive rate of bronchoscopic cytology and tissue biopsy were calculated respectively.The classification accuracy of cytological diagnosis for lung carcinoma was evaluated.In tissue biopsy standard,evaluated the significance of bronchoscopic cytology in diagnosis and histological diagnosis.Results The positive rate of bronchoscopic cytology and tissue biopsy were 86.1% (266/309) and 83.8% (259/309),respectively.Bronchoscopic cytology combined with bronchial biopsy could obviously improve the positive rate to 94.2% (291/309) in lung carcinoma diagnosis.Taking the tissue biopsy histological type as a standard,the cytotyping accuracy for brush method was 85.1%(74/87) in squamous carcinoma,82.4%(108/131) in adenocarcinoma and 100%(11/11) in small cell carcinoma for higher.However,the accuracy in diagnosing poorly differentiated carcinomas was only 12.2% (5/ 41).Conclusion Fiberoptic bronchoscopic brush cytology plays an stable and important role in diagnosing lung carcinomas and histological type determination.However,it has limited use in diagnosing poorly differentiated carcinomas.
10.Laparoscopic uterine artery blockage for the treatment of adenomyosis
Huicheng XU ; Zhiqing LIANG ; Yong CHEN ; Qing CHANG
Journal of Third Military Medical University 2003;0(22):-
Objective To assess the clinical efficacy of laparoscopic uterine artery blockage for the treatment of adenomyosis. Methods Laparoscopic uterine artery blockage was performed in 38 patients with adenomyosis. Enucleation and excision or vaporization of endometriosis were performed at the same time in patients with chocolate cyst of ovary and endometriosis. Lysis of adhesion and presacral neurectomy were performed in some patients. Symptoms and uterine volumes of all patients were investigated after the treatment. Results Relieved clinical symptoms were found in all 38 patients. Menorrhagia was reduced to (56?16)%. Complete disappearance of dysmenorrhea was found in 17 patients (44.7%), significantly alleviated in 14 patients (36.8%), partially alleviated in 6 patients (15.8%), but ineffectiveness in 1 patient (2.6%). Pain score and classification were decreased significantly (P