0.05). CAT expression of pCAT 1.28 and pCAT 0.65 are 2 and 1.6 times as high as the control group's. CAT expression of pCAT 1.28, pCAT 0.65 and pCAT 0.54 were 2.5, 2.2 and 1.3 times as high as the control group's. Aspirin inhibited the promoter activities of pCAT 1.28, pCAT 0.65. And the inhibition of Aspirin was reversed by PMA. CONGCLUSION:Aspirin can inhibit the expression of human MMP-9 gene in the transcriptional level and can inhibit the formation and development of prostatic carcinoma.

Objective To compare plasma levels of ANP in experimental and control groups and continuously explore the value of ANP in diagnosis and treatment of acute PTE. Methods Thirty guinea pigs were randomized to PTE group(n = 15) and control( n = 15) group. The plasma level of ANP was examined by radioimmunoassay.Moreover, pulmonary embolism(PE) was explored at dissection and on microscopic examination after PTE. Results (1)The plasma levels of ANP in the early phase of PTE group were significantly higher than those in control group(P＜0.05). (2)The plasma levels of ANP after PTE were significantly higher than those before PTE( P＜0.05). (3)In the late phase of PTE, the plasma levels of ANP in PTE group declined to normal levels, compared with control group, and there was no significant difference( P＞0.05). Conclusion (1) ANP, as a vasodilatory factor,was involved in mechanism of PTE,especially in the early phase of acute PTE. (2)The levels of ANP in plasma played as a sensitive marker used to observe conditions and seriousness of PTE.

Radiotherapy is an important method of tumor treatment .The incidence of complications caused by radiotherapy is high, which seriously effect patients′quality of life.The current terms of radiation enteritis , radiation proctitis and radiation cystitis can not reflect the changes of physiopathology after radiotherapy .Nowadays , a consensus has been gradually reached that 'pelvic radiation disease'is a more accurate description .This article mainly introduces the definition evolution , significance and current status of pelvic radiation disease to draw the attention of domestic related professionals .A new starting point is opening for the curing of radiotherapy-related complications .

Objective To investigate the role of induced sputum in monitoring airway inflammation of chronic obstructive pulmonary disease (COPD). Methods Induced sputum was taken from 26 patients with stable COPD. IL-8 level in the sputum was detected, and the lung function of the patients was determined. 15 healthy volunteers served as control group. Results The total cell count and the number of AM and PMN in sputum were significantly higher in the patients than those in the control group (P

Ocular surface diseases are common diseases in ophthalmology.It is difficult for clinicians to make early and accurate diagnosis based on symptoms, signs and serologic examination.Compared with the blood test, the tear test can reflect the condition of the local ocular surface and it has the characteristics of being non-invasive, having high diagnostic accuracy and fast diagnostic speed.Tear detection uses modern detection techniques such as polymerase chain reaction, enzyme-linked immunosorbent assay, gene chip and protein chip to detect the pathogen and immune components in tear, including microbial nucleic acid, tear antibody (IgM, IgG, IgE, IgA, antinuclear antibody, etc.) and cytokines (interleukin, tumor necrosis factor, interference factor, transforming growth factor, epidermal growth factor, etc.). Tear detection provides information about the pathogen infection and immune response on the ocular surface.At present, a large number of domestic and foreign tear detection studies show that there are certain regular changes in tear composition, not only in ocular surface diseases, uveitis, fundus diseases, thyroid related ophthalmopathy, but even in systemic diseases such as diabetes, hepatitis B, acquired immune deficiency syndrome etc.In addition, the research of tear pathogen and immune components is of great significance to study the cause, biochemical and immune process, treatment of diseases.In this paper, the latest research progress of tear collection, the clinical significance of tear pathogen and immune components and the characteristics of tear in various diseases was summarized and analyzed to provide reference for clinical practice.

Objective To investigate the association of esp, gelE, ebpA and QS-fsr system and biofilm formation in Enterococcus faecalis. Methods Totally 24 isolates of Enterococcus faecalis were collected from urine and catheter of clinical urine tract infection patients in Third Xiangya Hospital from Oct. 2007 to Jun. 2008, and were divided into biofilm group and non-biofilm group. The luminance ratios of esp, gelE, ebpA and fsrrB of Enterococcus faecalis in biofilm group and non-biofilm group were detected by RT-PCR. And the expression of esp, gelE, ebpA, fsrrB genes in different groups were detected by real-time PCR and were relatively quantitated through 2-△△Ct method. Moreover, the relevancies between that fourgenes and biofilm formation in Enterococcus faecalis were analyzed respectively. Results The expression of esp and ebpA in biofilm group were 298 times and 59 times more than the non-biofilm group. The expression level ofgelE and fsrB in biofilm group were 1/244 and 1/249 times less than the non-biofilm group, and the luminance ratios of esp, gelE, ebpA and fsrB were not significant between the two groups (rank sum was 92,79, 42 and 34 respectively,all P ＞ 0. 05 ). Conclusions The results showed that the biofilm formation in Enterococcus faecalis was promoted by esp and ebpA, and was inhabited by gelE and fsrB, which suggested that the expression of esp, ebpA and gelE genes was regulated by fsr system.

Objective To clarify the clinical distribution and antimicrobial resistance characteris-tic of bacterial biofilm during catheter-associated urinary tract infection,and to simulate biofilm "real state" in vivo. Methods Totally 120 patients with catheter-associated urinary tract infections (UTIs) were enrolled in the study. The urine specimens were collected for screening biofilm strains and the corresponding planktonic strains. The biofilm was detected with semi-quantitative detecting method. Antibiotics susceptibility test were performed on the biofilm bacteria to clarify the difference of drug resistance in common MH medium the between biofilm strains and the corresponding planktonic strains,as well as the difference of drug resistance of positive strains of the biofilm between Poloxamer medium and common MH medium. Results Totally 48 strains (48/120,40%) of biofilm bacteria were detected. The antibiotic susceptibility test of planktonic and biofilm bacteria in Mueller-Hinton agar showed no significant difference (P>0.05), while the antibiotic resistance of biofilm bacteria in Muel-ler-Hinton agar and Poloxamer hydrogel was statistically different (P<0.05),and the former was stronger. Conclusion The biofilm bacteria during the urinary tract infection were mainly Staphylococci and Enterococci. The antimicrobial resistance of planktonic and biofilm bacteria have no significant difference in vitro, h is speculated that Poloxamer media may simulate the real living environment of biofilm bacteria and display their "true" drug resistance.

It is confirmed that the adult cells can be re-programmed to embryonic stem cells(ESCs) by presenting some certain factors in oocytes in the clone process of animals. In recent years, some transcription factors that can induce pluripotent stem cells(iPS) have been identified and which made it possible to obtain induced pluripotent stem cells similar to embryonic stem cells. iPS provides a unique platform to study the pluripotent mechanism and to establish some specific disease models. This major scientific discovery can not only avoid the use of ES which involves ethics debate, but also lead the stem cell research to a new field.

[Objective]To evaluate the accuracy and influenceing factors of somatosensory evoked potential in spinal cord monitoring during cervical and thoracic spinal surgery and intraoperative nerve root monitoring in lumbar surgery.[Method]The somatosensory evoked potential(SEP) were used during arvical and thoracic spinal surgery and evaluated the accuracy of SEP according to the record of different stages and spinal cord function after surgery.The EMG were used to monitor the nerve root function in lumber operation to estimate whether nerve root being stimulated or tensioned.In addition,affected fators of SEP and EMG during operation were observed.[Result]Of 128 cases of cervical and thoracic surgery,116 cases did not reach the warning standards(amplitude decreasing 50% or diappearing) and showed no postoperative enhancement of symptom of nerve roots injury.12 cases reached the warning standards intraoperatively and the surgeon were warned to take some steps to finish the operations,only in one case incompletely transient paralysis occurred due to the time of amplitude decreasing of intraoperative SEP more than 10 minutes.Effect of other factors such as anaesthesia and low blood pressure did not reach the warning standards.There were 3 artifical negative cases.Only 1 was artifical positive case.of 40 cases of lumbar surgery,12 cases were found myoelectic responses,which warning the surgeon at any time to avoid nerve roots injury,no nerve roots injury were found after operation.[Conclusion]During cervical and thoracic spinal operation,the somatosensory evoked potential can reflect the physiological and pathological conditions of spinal cord after ruling out the interfering factors.Intraoperative spontaneous electromyography can reflect the nerve roots function promptly and accurately and assure the safety of lumbar surgery.

Objective To evaluate the clinical value of the quantitative detection of plasma 1,3-β-D-glucan in penicilliposis marneffei. Methods The 1,3-β-D-glucan concentrations were detected by using a G test. Results In 60 New Zealand rabbits infected by Penicillium marneffei,the levels of 1,3-β-D-glucan were positively correlated with infectious severity. The sensitivity, specificity, positive predictive rate and negative predictive rate were 88.3%、100%、 100% and 58.8% respectively,which were significantly higher than those of the control group(P <0.01 ).Conclusion The quantitative method for the detection of 1,3-β-D-glucan in plasma was simple,sensitive and reliable, and could be used as the assisting diagnosis of penicilliposis marneffei at early stage.