Objective:To improve orthotopic liver transplantation in the rat and conclude related operation skills.Methods:160 cases of orthotopic liver transplantation models in the rat were established by improved two-cuff technique.The anastomosis of portal vein between donor and recipient was performed by cuff technique.The same method was used to anastomose infrahepatic vena cava.The suprahepatic vena cava anastomosis was performed by suture and bile duct anastomosis was completed end to end by Teflon catheter.Results:The average time of donor operation,preparing graft and anhepatic period was 46min,24min and 21min respectively.The anastomosis time of suprahepatic vena cava,portal vein,infrahepatic vena cava, and bile duct was 13min,3min,4min,and 3min respectively.Main reasons for death during operation were pneumothorax,anesthesia and bleeding,and the successful rate of operation was 92.5%.Infection,obstruction of biliary tract and graft failure were attributed to the main causes for mortality after operation.Conclusion:Making orthotopic liver transplantation in the rat requires professional microsurgical techniques and careful operation.The improved two-cuff technique possesses advantages of short anhepatic phase,high operative successful rate,and is an ideal method for establishing this animal model.

Objective:To study the expression of Toll like receptor 4(TLR4) and MD-2 gene and protein in Kupffer cells and its role in ischemia-reperfusion injury (IRI) on rat liver graft.Methods:The Kupffer cells were isolated at 0(control group),2,12,24h (IR group)following IR.The mRNA and protein expressions of TLR4 and MD-2 were detected by RT-PCR and flow cytometry (FCM),respectively.The TNF-? level in supernatant was measured by ELISA.Then the isolated KCs were incubated with anti-TLR4 polyclonal antibody (anti-TLR4 group),and the TNF-? level were measured again.Results: The mRNA and protein expressions of TLR4/MD-2 and the level of TNF-? in IR group increased significantly following IR compared with those in control group( P

Curcumin is a principal polyphenolic curcuminoid extracted from turmeric rhizome, which has been used for treating inflammation of joints, ulcers, jaundice and other disorders in Asian traditional medicine. In recent years, many studies have indicated that curcumin plays important roles in treatment of liver diseases. Curcumin attenuates liver injury and non-alcoholic fatty liver disease by lowering the release of inflammation cytokines, minimizing oxidative stress, enhancing the sensitivity of insulin and altering lipid metabolism. Curcumin shows potent anti-fibrosis activity, contributing to inhibit the activation of hepatic stellate cells and reduce the deposition of extracellular matrix by its regulation of PPAR-γ, NF-ΚB and TGF-β signaling pathways. Moreover, curcumin exhibits anti-cancer effect by inducing G2/M phase cell cycle arrest and apoptosis in several hepatoma cell lines. However, poor water solubility and low bioavailability of curcumin limit its clinical applications. To overcome its limited systemic bioavailability, many new approaches have been explored to deliver curcumin effectively. This article focuses on advances in the effects of curcumin and its derivatives for treatment of liver injury, non-alcoholic fatty liver disease, liver fibrosis and hepatocarcinoma.

Objective To investigate the role of antigen-specific regulatory T cells in spontaneous diabetes of NOD mice. Methods Glutamic acid decarboxylase (GAD)-peptide specific T cells with fluorescein-labelled Class Ⅱ MHC tetramers were isolated by flow cytometer from NOD mice with spontaneous diabetes and diabetes resistant BALB/c mice. The cytokine profiles of these T cells were detected by antigen stimulated assay, ELISA and intracellular cytokine staining. Adoptively transferred diabetes was determined by intravenously injecting these T cells to NOD/scid mice. Results With different peptides working on the same strain, it showed that NOD mice-derived T cells secreted different amounts of interforn-? but comparable interlenkin (IL)-4 or IL-10, however, similar cytokine profiles were shown in BALB/c mice-derived T cells. With the same peptide working on different strains, NOD mice-derived T cells secreted less IL-2 but more IL-4 and IL-10 than BALB/c mice-derived T cells did. Interestingly, these NOD mice-derived T cells effectively inhibited diabetes development in adoptively transferred NOD/scid mice. Conclusion NOD mice-derived T cells inhibit development of diabetes, however, different cytokine profiles are expressed by these T cells induced by two adjacent GAD peptides. It suggests that these T cells are diabetes-inhibiting regulatory T cells displaying unique cytokine profiles.

Intestinal trefoil factor(IFF) is a low molecular weight polypeptide expressed in intestine.With the research on its structure and physiological function,ITF is beginning to attract attention.This review is mainly about the structure,localization,function,mechanism of action,expression regulation,signal transduction pathway,recombinant expression and so on.

Telemedicine is a combination of modern computer technology, communication technology and medical technology. It has a promising application potential. This paper mainly overviews the history of telemedicine, especially the development of wireless telemedicine. With the classification and comparison, the opinion on the future of wireless telemedicine is given according to the author's research work. Portable telemedicine system is the trend of telemedicine.

Curcumin is a principal polyphenolic curcuminoid extracted from turmeric rhizome, which has been used for treating inflammation of joints, ulcers, jaundice and other disorders in Asian traditional medicine. In recent years, many studies have indicated that curcumin plays important roles in treatment of liver diseases. Curcumin attenuates liver injury and non-alcoholic fatty liver disease by lowering the release of inflammation cytokines, minimizing oxidative stress, enhancing the sensitivity of insulin and altering lipid metabolism. Curcumin shows potent anti-fibrosis activity, contributing to inhibit the activation of hepatic stellate cells and reduce the deposition of extracellular matrix by its regulation of PPAR-γ, NF-ΚB and TGF-β signaling pathways. Moreover, curcumin exhibits anti-cancer effect by inducing G2/M phase cell cycle arrest and apoptosis in several hepatoma cell lines. However, poor water solubility and low bioavailability of curcumin limit its clinical applications. To overcome its limited systemic bioavailability, many new approaches have been explored to deliver curcumin effectively. This article focuses on advances in the effects of curcumin and its derivatives for treatment of liver injury, non-alcoholic fatty liver disease, liver fibrosis and hepatocarcinoma.
Apoptosis ; Cell Cycle Checkpoints ; drug effects ; Cell Line, Tumor ; Curcumin ; pharmacology ; Hepatic Stellate Cells ; drug effects ; Humans ; Inflammation ; drug therapy ; Liver Diseases ; drug therapy ; NF-kappa B ; metabolism ; Oxidative Stress ; drug effects ; PPAR gamma ; metabolism ; Signal Transduction ; Transforming Growth Factor beta ; metabolism

One case of Klinefelter syndrome with complicated 46,XY/47,XXY/48,XXXXY and metabolic syndrome was reported.

Purpose To explore the value of postoperative stimulated thyroglobulin (ps-Tg) level in predicting functional metastasis in patients with differentiated thyroid carcinoma (DTC) before 131I therapy,in order to guide 131I therapy.Materials and Methods 101 DTC patients who accepted total thyroidectomy and lymphadenectomy in the First People's Hospital of Lianyungang were retrospectively analyzed.The ps-Tg level after DTC surgery was detected 1 day before 131I therapy,and 131I-SPECT/CT scan was performed 5-7 days after 131I therapy.The presence of functional metastasis was confirmed on the basis of 131I-SPECT/CT imaging,and the patients were assigned into non-metastic (M0) group and metastatic (M1) group.The ROC curve was used to evaluate the value of ps-Tg in predicting functional metastasis.Results The ps-Tg level in M 1 group was higher than that in M0 group,and the difference was statistically significant (U=328.00,P＜0.001).The area of ps-Tg value under the ROC curve was 0.870,and the diagnostic cut-off point was 40.60 ng/ml.The sensitivity,specificity and accuracy was 72.34％,100.00％ and 87.13％,respectively.Concltsion The ps-Tg value can be used as an effective indicator to predict functional metastasis,and is able to guide 131I therapy.

Objective To investigate the protective effect and mechanisms of NF-?B decoy oli- godeoxynucleotides(ODNs)on rat liver graft following ischemia-reperfusion injury.Methods Ani- mals were randomly divided into 3 groups(n=8 in each group):control group,ischemia-reperfusion (IR)group,and decoy ODNs group,in which donor grafts were transfected with 120?g NF-?B decoy ODNs before graft procurement.Following 2 h of reperfusion,Kupffer cells(KCs)were isolated,and NF-?B biding activity was detected with electrophoretic mobility shift assay(EMSA),and TNF-?、IL- 6 mRNA expression was assayed by using RT-PCR method.Meanwhile,the liver and serum samples were collected,the histopathologic change of liver was examined by light microscope,and liver func- tion was analyzed.Results The NF-?B biding activity,TNF-?,IL-6 mRNA expression,and the ALT,TBIL levels in serum were significantly increased following 2 h of reperfusion in IR group as compared with those in control group(P＜0.01).A large amount of degeneration and necrosis in hep- atocytes accompanied with obvious congestion in hepatic sinusoid occurred.However,these tested in- dexes were significantly ameliorated in decoy ODNs group as compared with those in IR group(P＜0.01),and the architecture of hepatic lobules was remained.Conclusions KCs NF-?B activation fol- lowing reperfusion plays an important role in IRI in liver transplantation.Decoy strategy shows appar- ent effect on suppression of NF-?B activation,and thus inhibits the production of downstream cyto- kines,which protects the liver graft from IRI.