1.Pulmonary Fungal Infection and Resistance in Older Chronic Obstructive Pulmonary Disease Patients with Mechanical Ventilation
Chinese Journal of Nosocomiology 2009;0(20):-
OBJECTIVE To investigate pulmonary fungal infection spectrum and its resistance in older COPD patients with mechanical ventilation.METHODS Retrospective study were performed in 67 cases of COPD patients with mechanical ventilation treatment who were admitted to our ICU from Jan 2004 to Doc 2007.Bronchoalveolar lavage fluid was cultured,differentiation and antifungal susceptilibity testing were taken.RESULTS Seventy-eight fungi strains were cultured in 78 cases,among them,41 strains were Candida albicans(52.56%),14 of C.tropicalis,9 of C.krusei,5 of C.parapsilosis,7 of yeast-like fungi and 2 of Aspergillus.CONCLUSIONS There were increasing fungal infection rate in older COPD patients with mechanical ventilation and C.albicans is still the most frequent type.The resistance to triazole antifungal drug is also increasing.
2.Molecular genetics and prognosis of lung cancer in young patients: Research highlights
Medical Journal of Chinese People's Liberation Army 2017;42(3):181-185
Differed from the elderly patients with lung cancer,the younger patients with lung cancer,less than 50 years old,present unique clinical features.Recently,the incidence of lung cancer in young people has shown a rising trend,making the research on this field more valuable.At present,molecular targeted therapy is one of the most popular areas of non-small-cell lung cancer (NSCLC),and researches are focused on the epidermal growth factor receptor (EGFR) and echinoderm microtubule associated protein like4-anaplastic lymphoma kinase (EML4-ALK).In addition,the previous researches revealed the differences between the young and elderly patients with lung cancer on molecular genetics and prognosis,so the researches on prognostic factors for young patients with lung cancer are of great clinical significance.The present paper will focus on the aspects of pathogenesis,molecular genetics and prognosis in young patients with lung cancer.
3.Ouabain and asthenospermia.
National Journal of Andrology 2015;21(12):1129-1133
Asthenospermia accounts for about 30% of the causes of male infertility. Currently, most drugs for asthenospermia lack specificity and desirable therapeutic efficiency. An insight into the pathogenesis of asthenospermia is important for the development of specific therapies for this disease. The protein Na+/K(+)- ATPase α4 isoform (NKA4) presents in both mature testis tissue and the sperm tail, the absence or reduced activity of which may significantly decrease sperm motility. Ouabain is a natural inhibitor of NKA4, suppressing its activity by specifically binding the ouabain site in it. The hypothalamus and adrenal cortex excrete an ouabain-like steroid hormone called endogenous ouabain (EO), which may be associated with the pathogenesis of asthenospermia by inhibiting the activity of NKA4, affecting Na+/H+ exchange, Na+/Ca2+ exchange and sperm cell membrane potential, and eventually reducing sperm motility.
Asthenozoospermia
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physiopathology
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Humans
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Male
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Membrane Potentials
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Ouabain
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chemistry
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Protein Isoforms
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Sodium-Potassium-Exchanging ATPase
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antagonists & inhibitors
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Sperm Motility
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Sperm Tail
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Spermatozoa
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Testis
5.Analysis of the effect of rehabilitation on fluorspar miners with pneumoconiosis treated by non-invasive ventilator.
Ye PAN ; Yong-gang WU ; Guo-wu PAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(6):469-470
Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Mining
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Noninvasive Ventilation
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Pneumoconiosis
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rehabilitation
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Treatment Outcome
6.Research progress of nano delivery system in mRNA tumor vaccines
Pan-pan GU ; Tong GAO ; Yong-jun LIU ; Na ZHANG
Acta Pharmaceutica Sinica 2022;57(8):2327-2333
Tumor vaccine is one of the most promising therapeutic strategies in tumor immunotherapy. It promotes the antigen presentation process by delivering tumor antigen and then activates the anti-tumor immune response. As a new class of vaccines, messenger RNA (mRNA) vaccines can activate the immune system to achieve the purpose of immunotherapy by delivering the mRNA sequence of a specific antigen into the body and expressing the corresponding antigen protein. Compared with traditional vaccines, mRNA vaccines have the advantages of a short production cycle, high effectiveness, and strong immunogenicity. In recent years, the application of mRNA vaccines in tumor immunotherapy has attracted widespread attention, but the instability and low delivery efficiency of mRNA limit its application. Nano delivery system can effectively solve the problem of mRNA vaccine delivery, greatly promote the research process and clinical application of mRNA tumor vaccines, and has become a hot spot in the research of mRNA vaccines. In this review, we introduced the mRNA tumor vaccines, focusing on the application of nano delivery system in mRNA tumor vaccines, in order to provide new ideas and new methods for the efficient delivery of mRNA tumor vaccines and tumor immunotherapy.
7.Comparative analysis of incomplete Kawasaki disease and typical Kawasaki disease
Lilin ZHENG ; Yong LYU ; Jiahua PAN
Chinese Journal of Primary Medicine and Pharmacy 2014;21(2):186-187
Objective To investigate the similarities and differences of incomplete Kawasaki disease and typical Kawasaki disease,in order to provide basis for early diagnosis and treatment.Methods The clinical and laboratory data of 60 children with Kawasaki disease were retrospectively analyzed.Results The incidence rate of symptoms in clinical diagnostic criteria was lower in incomplete Kawasaki disease than that in typical Kawasaki disease(x2 =16.46,10.10,11.71,34.43,all P < 0.01).No statistical differences of leukocytes,platelet,erythrocyte sedim-entation rate and C-reactive protein were found in the incomplete Kawasaki disease and typical Kawasaki disease(x2 =0.04,0.12,0.04,0.26,all P > 0.05).The incidence rate of coronary artery lesions had significant difference between incomplete Kawasaki disease and typical Kawasaki disease (x2 =31.43,P < 0.01).Conclusion The early diagnosis of Kawasaki disease could be confirmed by representative clinical characteristics,laboratory examination and ultrasound cardiogram.
8.Polarographic and Voltammetric Behavior of Cadmium(Ⅱ)-meso-tetrakis (p-sulfophenyl)porphyrin Complex
Xiaoping WANG ; Yong ZHANG ; Jinghao PAN
Chinese Journal of Analytical Chemistry 2001;29(3):290-292
The Polarographic and voltammetric behavior of Cd (Ⅱ)-meso-tetrakis(p-sulfophenyl)prophyrin (TPPS4) complex was defined and the cyclic voltammetric peak was obtained at 1.200 V (vs. SCE) in 0.3 mol/L NaoH solution. The characteristics of the peak have been examined in detail and the experimental results prove that the peak has adsorption properties. In addition to the number of electrons transferred in the reaction and the complex ratio, the stability const ant (β=1.19×107) was calculated. The peak current was proportional to the concentration of Cd (Ⅱ) over the rang 3×10-7~1×10-5 mol/L and the limit of detection was 6×10-8 mol/L. Furthermore a possible mechanism was proposed for the reduction of the complex.
9.Application of RAPD Molecular Marker in the Phylogenetic Analysis of Soy Sauce Strains
Bin WANG ; Li PAN ; Yong GUO ;
Microbiology 1992;0(03):-
RAPD analysis of eight soy sauce strains isolated from commercial soy sauce koji was done with random primers,using Aspergillus oryzae AS3.951 and Aspergillus sojae AS3.495 as controls.Six appropriate primers(Primer1,Primer5,Primer6,Primer7,Primer8,Primer9)for RAPD-PCR were screened from nine random primers,and repetitive experiments demonstrated that their RAPD-PCR polymorphic patterns were stable.There were usually 4~8 bands in their RADP-PCR patterns,where the number of the main bands was 1~4 and the secondary bands were abundant.The phylogenetic tree of these strains was reconstructed according to their RAPD-PCR patterns,and it basically corresponded to traditional morphological taxology,demonstrating that the application of RAPD molecular marker in the phylogenetic analysis of soy sauce strains was feasible.
10.The application of internal anastomotic appliance of pouch pliers in the 65 cases retention of anus operation of mesal,low rectal carcinoma
Xiang-Yong ZENG ; Fu-Quan PAN ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To investigate the application of a new type internal anastomotic appliance of pouch pliers in the retention of anus operation of mesal,low rectal carcinoma.Methods The data of 65 patients with mesal,low rectal carcinoma with the technique of the anal retention using KYGW type anastomotic appliance of pouch pliers were reviewed and analyzed retrospectively.Results 65 patients were coincided success only one time. Anastomotic fistula occurred after operation in 1 case,being cured and discharged at last,no anastomotic stricture. Conclusion The internal anastomotic appliance of pouch pliers in the anal retention of mesal-low rectal carcinoma is an efficient method with better cost-effectiveness and fewer complications,which is easy to manipulate and popular- ize.