Differed from the elderly patients with lung cancer,the younger patients with lung cancer,less than 50 years old,present unique clinical features.Recently,the incidence of lung cancer in young people has shown a rising trend,making the research on this field more valuable.At present,molecular targeted therapy is one of the most popular areas of non-small-cell lung cancer (NSCLC),and researches are focused on the epidermal growth factor receptor (EGFR) and echinoderm microtubule associated protein like4-anaplastic lymphoma kinase (EML4-ALK).In addition,the previous researches revealed the differences between the young and elderly patients with lung cancer on molecular genetics and prognosis,so the researches on prognostic factors for young patients with lung cancer are of great clinical significance.The present paper will focus on the aspects of pathogenesis,molecular genetics and prognosis in young patients with lung cancer.

OBJECTIVE To investigate pulmonary fungal infection spectrum and its resistance in older COPD patients with mechanical ventilation.METHODS Retrospective study were performed in 67 cases of COPD patients with mechanical ventilation treatment who were admitted to our ICU from Jan 2004 to Doc 2007.Bronchoalveolar lavage fluid was cultured,differentiation and antifungal susceptilibity testing were taken.RESULTS Seventy-eight fungi strains were cultured in 78 cases,among them,41 strains were Candida albicans(52.56%),14 of C.tropicalis,9 of C.krusei,5 of C.parapsilosis,7 of yeast-like fungi and 2 of Aspergillus.CONCLUSIONS There were increasing fungal infection rate in older COPD patients with mechanical ventilation and C.albicans is still the most frequent type.The resistance to triazole antifungal drug is also increasing.

Asthenospermia accounts for about 30% of the causes of male infertility. Currently, most drugs for asthenospermia lack specificity and desirable therapeutic efficiency. An insight into the pathogenesis of asthenospermia is important for the development of specific therapies for this disease. The protein Na+/K(+)- ATPase α4 isoform (NKA4) presents in both mature testis tissue and the sperm tail, the absence or reduced activity of which may significantly decrease sperm motility. Ouabain is a natural inhibitor of NKA4, suppressing its activity by specifically binding the ouabain site in it. The hypothalamus and adrenal cortex excrete an ouabain-like steroid hormone called endogenous ouabain (EO), which may be associated with the pathogenesis of asthenospermia by inhibiting the activity of NKA4, affecting Na+/H+ exchange, Na+/Ca2+ exchange and sperm cell membrane potential, and eventually reducing sperm motility.
Asthenozoospermia ; physiopathology ; Humans ; Male ; Membrane Potentials ; Ouabain ; chemistry ; Protein Isoforms ; Sodium-Potassium-Exchanging ATPase ; antagonists & inhibitors ; Sperm Motility ; Sperm Tail ; Spermatozoa ; Testis

History, 19th Century ; History, 20th Century ; Humans ; Neurosurgery ; history ; Philately ; United States

Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Mining ; Noninvasive Ventilation ; Pneumoconiosis ; rehabilitation ; Treatment Outcome

Tumor vaccine is one of the most promising therapeutic strategies in tumor immunotherapy. It promotes the antigen presentation process by delivering tumor antigen and then activates the anti-tumor immune response. As a new class of vaccines, messenger RNA (mRNA) vaccines can activate the immune system to achieve the purpose of immunotherapy by delivering the mRNA sequence of a specific antigen into the body and expressing the corresponding antigen protein. Compared with traditional vaccines, mRNA vaccines have the advantages of a short production cycle, high effectiveness, and strong immunogenicity. In recent years, the application of mRNA vaccines in tumor immunotherapy has attracted widespread attention, but the instability and low delivery efficiency of mRNA limit its application. Nano delivery system can effectively solve the problem of mRNA vaccine delivery, greatly promote the research process and clinical application of mRNA tumor vaccines, and has become a hot spot in the research of mRNA vaccines. In this review, we introduced the mRNA tumor vaccines, focusing on the application of nano delivery system in mRNA tumor vaccines, in order to provide new ideas and new methods for the efficient delivery of mRNA tumor vaccines and tumor immunotherapy.

Objective:To investigate the clinical efficacy of recombinant human interferon α-2b gels combined with ulcer powders for external use in the treatment of cervicitis patients with human papillomavirus infection. Methods: Totally 76 cases of cervicitis patients with human papillomavirus infection were randomly divided into the observation group and the control group. The observation group was treated by recombinant human interferonα-2b gels combined with ulcer powders for external use through vaginal administration, once every 2d on bedtime. The control group was treated only by ulcer powders for external use. After the 6-month treatment, the HPV negative conversion rate, clinical efficacy and adverse drug reactions in the two groups were detected. Results:After the treatment, the HPV negative conversion rate of the observation group was 83. 33%, which was higher than that of the control group (60. 00%, P <0. 001). The total effective rate of the observation group was 88. 89%, which was higher than that of the control group (82. 50%, P < 0. 05). Conclusion: The clinical efficacy of recombinant human interferon α-2b gels combined with ulcer powders for external use in the treatment of cervicitis patients with human papillomavirus infection is significant, which is worthy of wider application.

Background and purpose:Regular physical exam became the main means to discover the population with early stage RCC. So far, there have been no reports with large sample that have been published about the biomarkers as predictor for the metastasis of the postoperative patients with stage T_(1-3)N_0M_0 (RCC) in China. Furthermore, the underlying mechanism of metastasis of RCC is not clear. This research was carried out in order to study the correlation between delayed metastasis of early stage renal cell carcinoma after surgical operation and expressions of KAI-1、Ki-67 and HER-2/neu on RCC. Methods:Two hundred and forty-one patients with RCC underwent surgical operations and were pathologically diagnosed as T_(1-3)N_0M_0 stage. Twenty-four patients were found to have metastases after long-term follow-up and their clinical data were reviewed (metastasis group). One hundred and ninety-four patients without postoperative metastases were taken as control group. Twenty three patients were excluded from this study because they were lost to follow-up. The expressions of KAI1、 Ki-67 and HER-2/neu in the samples of the two groups were tested with immunohistochemical staining by PowerVision or EnVision two-step procedure. Significant difference was calculated with Chi square test.Results:The positive expression rates of KAI1、HER-2/neu and Ki-67 in 218 RCC were 82.6%、27.5% and 83.5%, respectively. Both mild (20.8%) and strong positive rates (4.2%) of KAI1 in metastasis group were dramatically less than that in control group (90.2%, 73.7%, P

Objective To observe the characteristics of coronary hemodynamics in acute hibernating myocardium (AHM) model by intracoronary doppler guidewire. Methods 13 little domestic Chinese swine were established to the model of AHM by interventional method (closed chest),intracoronary Doppler guidewire were used to measure average peak velocity (APV),diastolic systolic velocity ratio (DSVR) and coronary flow velocity reserve (CFVR) distal to the stenosis in the period of pre model,post model and reperfusion.Then animals were executed,the histomography changes of AHM cells were observed.Results 10 animals (76 92%) were established to the model of AHM successfully,myocardium necrosis were not found under microscopical examination.APV and CFVR distal to the stenosis decreased significantly in AHM model ( P

The Polarographic and voltammetric behavior of Cd (Ⅱ)-meso-tetrakis(p-sulfophenyl)prophyrin (TPPS4） complex was defined and the cyclic voltammetric peak was obtained at 1.200 V (vs. SCE) in 0.3 mol/L NaoH solution. The characteristics of the peak have been examined in detail and the experimental results prove that the peak has adsorption properties. In addition to the number of electrons transferred in the reaction and the complex ratio, the stability const ant (β=1.19×107) was calculated. The peak current was proportional to the concentration of Cd (Ⅱ) over the rang 3×10－7～1×10－5 mol/L and the limit of detection was 6×10－8 mol/L. Furthermore a possible mechanism was proposed for the reduction of the complex.