1.Human Cloning.
Journal of the Korean Medical Association 1999;42(4):369-374
No abstract available.
Cloning, Organism*
;
Humans*
2.Human Cloning: Hope or Disaster.
Journal of the Korean Medical Association 2003;46(4):264-265
No abstract available.
Cloning, Organism*
;
Disasters*
;
Hope*
3.Delayed Type of Bilateral Tubal Pregnancy.
Journal of the Korean Medical Association 1998;41(9):973-977
No abstract available.
Female
;
Pregnancy
;
Pregnancy, Tubal*
4.Apoptosis and Expression of bcl-2, bax in the Cyclic Human Endometrium.
Chang Suk SUH ; Shin Yong MOON
Korean Journal of Obstetrics and Gynecology 1999;42(11):2446-2457
OBJECTIVE: To understand the regulatory mechanism of apoptosis by pro- and anti-apoptotic genes in the cyclic human endometrium. METHODS: Each case of endometrial status was classified by Noyes criteria, and grouped into early proliferative(n=13), late proliferative(n=14), early secretory(n=15), and late secretory phases(n=15). Expression of bcl-2, and bax were assessed by Northern blot and immunohistochemistry in relation to apoptotic index by TUNEL. Results: Apoptotic index showed increasing tendency as progressing to the late secretory phase, which phase showed significantly higher apoptotic index compared to the other phases(p<0.05). The intensity of bcl-2 8.5kb transcript by Northern blot was highest in the late proliferative phase significantly(p<0.05), decreasing to nadir in the late secretory phase. In contrast to bcl-2 expression, bax mRNA expression was highest in the late secretory phase significantly(p<0.05). Both the relative ratio of bcl-2 8.5kb transcript and bcl-2 5.5kb trascript to bax showed that the ratio was higher in the early, and late proliferative phase, but, reversed in the late secretory phase. Both the immunoreactivity of bcl-2 and bax proteins could be detected in the basal, functional, and stromal layers of endometrium. The immunoreactivity of bcl-2 protein was more prominent in the proliferative phase, however, bax protein was more prominent in the secretory phase. CONCLUSION: This study suggests that apoptosis could be regulated by the relative dominance of bcl-2 or bax expression in the human endometrium. Thus, bcl-2 and bax expressions might be one of the possible mechanism in the regulation of normal menstruation.
Apoptosis*
;
bcl-2-Associated X Protein
;
Blotting, Northern
;
Endometrium*
;
Female
;
Humans*
;
Immunohistochemistry
;
In Situ Nick-End Labeling
;
Menstruation
;
RNA, Messenger
5.Hypo-osmotic swelling test as a predictor for nuclear maturity of sperms assessed by aniline blue staining.
Byung Chul JEE ; Shin Yong MOON
Korean Journal of Obstetrics and Gynecology 1999;42(8):1782-1788
OBJECTIVE: Many cases of fertilization failure after ICSI is attributed to decondensation defect of sperm head, therefore, selection of sperm with well-condensed, fully-matured nucleus is critical for successful fertilization. During mammalian spermiogenesis, replacement of the somatic histones by protamines allows normal sperm nuclear condensation. The degree of condensation can be shown with the aid of acidic aniline blue staining, which is able to discriminate between lysine-rich histones and arginine- or cysteine-rich protamines. The objective of this study was to investigate the possible relation between nuclear maturity of human sperm and hypo-osmotic swelling patterns. Hypo-osmotic swelling test is very simple and convenient test to measure functional integrity of human sperm membrane, and each already tested sperms can be used for fertilization. METHODS: For assessment of sperm nuclear maturity, aniline blue staining was performed on the slides, already undergone hypo-osmotic swelling test in 5 subfertile patients with abnormal semen parameters and 8 fertile donors. After 3 hours swim-up, these procedures were repeated in each cases. RESULTS: Sperms with negative aniline blue were significantly increased after swim-up in each group. There was a significant increase in the proportion of negative aniline blue in sperms with swelling patterns(e+f+g) compared to sperms with non-swelling patterns(a), except swim-up group in subfertile patients. CONCLUSION: Choosing the swelling patterns, rather than non-swelling sperms after hypo-osmotic test may be beneficial for the selection of sperms with well-condensed, mature nucleus and intact membrane.
Fertilization
;
Histones
;
Humans
;
Membranes
;
Protamines
;
Semen
;
Sperm Head
;
Sperm Injections, Intracytoplasmic
;
Spermatogenesis
;
Spermatozoa*
;
Tissue Donors
6.The Effects of Isotypes and Regional Distribution of Antisperm Antibodies on Semen Parameters and Fertilizing Ability.
Myung Geol PANG ; Shin Yong MOON
Korean Journal of Fertility and Sterility 1998;25(1):1-8
SUMMARY: To investigate the influences on semen parameters and fertilizing capacity of immuno- globulin(Ig) isotypes and regional distribution of antisperm antibody (ASA) on the human sperm surface. Sixty-seven ASA-positive patients were compared with 96 ASA-negative donors. ASAs in semen showed significant negative effects on both semen parameters and fertilizing capacity; in those with ASAs in the sperm head and/or tail, the reductions were significant. In the head as well as the tail, there was close correlation between fertilizing capacity and both IgG and IgA. Both semen parameters and fertilizing capacity are significantly affected by the presence of ASA in semen. In particular, antibodies IgG to sperm head and/or tail, and antibodies IgA to sperm tail appeared to have a highly detrimental effect on fertilizing capacity.
Antibodies*
;
Head
;
Humans
;
Immunoglobulin A
;
Immunoglobulin G
;
Semen*
;
Sperm Head
;
Sperm Tail
;
Spermatozoa
;
Tissue Donors
7.Causes of Chronic Headache in Children.
Han Koo MOON ; Son Moon SHIN ; Yong Hoon PARK
Journal of the Korean Pediatric Society 1990;33(12):1680-1688
No abstract available.
Child*
;
Headache Disorders*
;
Humans
8.The effective of ultrashort-term gonadotropin-releasing hormone agonist administration in controlled ovarian hyperstimulation for in vitro fertilization.
Chi Seok AHN ; Shin Yong MOON ; Yoon Seok CHANG
Korean Journal of Obstetrics and Gynecology 1993;36(7):2633-2644
No abstract available.
Fertilization in Vitro*
;
Gonadotropin-Releasing Hormone*
9.A Clinical Study on Children with Acute Glomerulonephritis.
Jeong Ok KIM ; Son Moon SHIN ; Yong Hoon PARK
Journal of the Korean Pediatric Society 1989;32(6):779-788
No abstract available.
Child*
;
Glomerulonephritis*
;
Humans
10.Detection and clinical significances of the occurrence of endogenous LH surge with enzyme immunoassay and fluoroimmunoassay.
Jong Kwan JUN ; Shin Yong MOON ; Yoon Seok CHANG
Korean Journal of Obstetrics and Gynecology 1991;34(7):961-971
No abstract available.
Fluoroimmunoassay*
;
Immunoenzyme Techniques*