The cytologic heterogeneity of the tumor cells in gastric signet ring cell carcinoma was studied with 13 surgically early gastric carcinoma specimens by means of histochemical stainings on mucin(periodic acid Schiff-alcian blue at pH 2.5, paradoxical concanavalin A, high iron diamine) and electron microscopy. Of the 13 cases of early gastric cell carcinomas, 6 were mucosal type and 7 were submucosal type. Eleven cases consisted of mixture of gastric and intestinal type signet ring cells and the remaining 2 of the mucosal type were entirely made of gastric type. The colonic goblet cell type was found in 4 of the submucosal type. Within the mucosa the tumor cells showed a layering phenomenon; type A signet ring cells were distributed at the central zone and type B and C at the superficial or deeper zone. Each type of signet ring cell showed variable mucin histochemical stainability of gastric and/or intestinal nature. Above finding strongly suggest that the variable phenotypes of signet ring cells result from a heterogeneity of cytoplasmic mucin as well as different stages of differentiation of signet ring carcinoma cells.

Two cases of duodenal bezoars are presented. Both were within pseudodiverticula of the duodenal bulbs whichwere created by chronic active ulcer disease. It is felt that deformed duodenal bulb interfered with transit ofthe gastrointestinal content causing the formation of bezoar within pseudodiverticula.
Bezoars ; Gastrointestinal Contents ; Ulcer

No abstract available.
Body Temperature*

We analysed 8 cases of gastrointestinal cytomegalovirus(CMV) infection including one autopsy, three surgically resected and four endoscopic biopsy cases. Involved sites were colon in four,stomach in three and small intestine in one. Three of them were associated with immu-nosuppressed condition but others had no clinical evidence of immune deficiency. Multiple aphthous mucosal ulceration was a common presentation, but one revealed a mucosal ulcer with segmental narrowing and thickening of wall. Microscopically, six showed cytomegalic inclusions in endothelial cells and fibroblasts, one in mucosal epithelial cells and the remaining one in both endothelial cells and mucosal epithelial cells. Immunohistochemical staining using monoclonal antibody against CMV confirmed postive result in seven cases. Serum IgM anti-CMV antibody was elevated in one case. We conclude that gastrointestinal CMV infection is currently not a rare condition and frequently associated with non-immunosuppressed condition, and thus a thorough histologic examination is required especially in the gastrointestinal ulcerative lesion. Once cytomegalic inclusion is suspected, immunohistochemical identification of CMV seems essential for specific diagnosis.
Biopsy

To assess the prognostic factors of the femoral neck fractures in patients with CVA, we compared the survival rates in 12 cases of CVA group with 12 cases of non-CVA group and evaluated the various clinical factors of CVA group affect the mortality. All of the patients both CVA group and non- CVA group were treated hemiarthroplasty for femoral neck fractures between June 1990 and June 1992. The mean age was 68.9 years old in the CVA group and 69.3 years old in the non-CVA group. The mean follow-up periods were 32.8 months in the CVA group and 33.6 months in the non-CVA group. The results were as follows; 1. The incidence of the femoral neck fracture with CVA was 15%(12 cases among SO femoral neck fractures). 2. The survival rates in the CVA group at l-year, 2-year, and 3-year were 83.3%, 66.7%, and 58.3% respectively. In the non-CVA group, the survival rates were 83.3%, 75.0%, and 62.5%. 3. In the CVA group, serum total protein, nutritional index, hypertension, lung disease, and cardiac disease may be valuable prognostic factors. In conclusion, the pretraumatic patient's general physical condition could affect the mortality of femoral neck fracture in the CVA patients rather than CVA itself or postoperative status.
Femoral Neck Fractures ; Femur Neck ; Follow-Up Studies ; Heart Diseases ; Hemiarthroplasty ; Humans ; Hypertension ; Incidence ; Lung Diseases ; Mortality ; Nutrition Assessment ; Stroke ; Survival Rate

We performed dual color fluorescence in situ hybridization (FISH) for the bcr/abl fusion in CML using the peripheral blood smears without destruction of cell morphology to determine the bcr/abl fusion. Two patients of CML, one patient in accelerated phase and one patient in chronic phase, were selected. The blood smears were fixed in absolute methanol. FISH was performed with the Mbcr/abl translocation DNA probe mixture and the slides were stained with Wright's stain after FISH. The blood smears of both cases revealed distinct signals without destruction of cellular morphology. The normoblasts and lymphocytes revealed beautiful fused bcr/abl signals as well as granulocytes in both cases. The results provide a novel finding that the normoblasts and lymphocytes in CML are also neoplastic clonal cells which has not been demonstrated with a single-cell approach before.
DNA ; Erythroblasts* ; Fluorescence* ; Granulocytes ; Humans ; In Situ Hybridization ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive* ; Lymphocytes* ; Methanol

Four children, with histopathologically confirmed posterior fossa primitive neuroectoderrnal tumors, were examined by plain radiography, computed tomography (CT) and cerebral angiography. The homogeneously well enhanced solid mass in the midline of the posterior fossa and hydrocephalus of various degree were seen on all CT scans. One case had calcifications and another case had low density areas in the tumor mass. Three cerebral angiograms showed vascular displacement without tumor vascularities. Unfortunately, these CT and angiography findings are and other tumors. when a well enhanced solid mass in the midline posterior fossa is seen on CT scan in children.
Angiography ; Cerebral Angiography ; Child ; Humans ; Hydrocephalus ; Medulloblastoma* ; Neuroectodermal Tumors, Primitive* ; Pathology* ; Radiography ; Tomography, X-Ray Computed