No abstract available.
Constriction, Pathologic* ; Fetal Death*

As one of many cell-many cell-based cartilage repairing methods, transplantation of chondrocyte-embedded-collagen gels in cartilage defect was performed for more satisfactory regeneration of cartilage. The authors performed this study to investigate whether the TGF-beta1 treatment of chondrocytes can do some additional synergistic effect on the transplantation of chondrocyte-embedded-collagen gels for crtilage repair. Chondrocytes were isolated from the articular cartilage of newborn Sprague-Dawley rats. Chondrocytes cultured for 10 days in monolayer were embedded in the 0.45% type I collagen gel. Full-thickness cartilage defect was made in the patellar groove of adult Sprague-Dawley rats. Chondrocytes culdefect was made in the patellar groove of adult Sprague-Dawley rats. The cartilage defects were treated with the following methods in a total of 200 animals, which were assigned to 5 different groups of 40 rats. In the control group, the deffect was left without any treatment, in group I, the defect was filled with collagen gel only, in group II, with collagen gel coontaining 10 ng/ml concentration of TGF-beta1, in group III, with collagen gel containing chondrocytes, and in group IV, with collagen gel containing chondrocytes and TGF-beta1. At 1, 2, 4, 8, 12 weeks after the operation, eight rats of each group were sacrificed, and their distal femurs were harvested for the histologic and biomechanical tests. The section s were stained with hematoxilin and eosin. Alcian-blue, and Safranin-O. Regenerated cartilage was analyzed by the semiquantitative histological grading system. Point indentation test was performed as a biomechanical evaluation, and the stiffness was calculated. The results of the histological grading system revealed that the scores gradually increased with time in all groups, and the scores of group III and IV were higher than those of control, group I and II. The biomechanical study showed that the stiffness gradually increased to reach a plateau level in each group. In control, group I and II, the stiffness increased up to the eighth week and remained around the increased level at the twelfth week, and did not show any statistically significant difference between the groups. In group III and IV, the stiffness was higher than in control group, and increased markedly at the fourth week and the increased level was maintained onwards. The results of this study showed that the transplantation of chondrocyte-embedded-collagen gels enhanced the healing process, and the treatment of TGF-beta1 demonstrated at least partially significant improvement.
Adult ; Animals ; Cartilage ; Cartilage, Articular* ; Chondrocytes ; Collagen ; Collagen Type I ; Eosine Yellowish-(YS) ; Femur ; Gels ; Humans ; Infant, Newborn ; Rats* ; Rats, Sprague-Dawley ; Regeneration ; Transforming Growth Factor beta1

No abstract available.
Pregnancy* ; Ultrasonography*

The mechanical stiffness of Unilateral-Ring Hybrid External Fixation(URHEF) was analyzed and compared with conventional Ilizarov circular external fixation system and unilateral fixation with DynaExtor(R). Our URHEF is basically a unilateral external fixation system which affords easier incorporation and removal of the Ilizarov rings(200mm in diameter) coupled with connecting clamps at anytime, if needed. It allows controlled axial micromotion in due time. For mechanical test, URHEF system was constructed using a DynaExtor(R) fixed with two or three half pins(6 mm in diameter) attached with two proximal and distal Ilizarov rings fixed with an olive pin and a half pin on each ring. Unilateral fixation system was constructed with a DynaExtor(R) fixed with 2 or 3 half pins. Ring fixation system was constructed with four Ilizarov rings(140mm in diameter). Ilizarov system were assembled into 90degrees-90degrees and 45degrees-13 5degrees configurations with 2 olive pins, respectively. The distance between the center of pylon and the pin-clamp interface was equalized, being 70mm, in all the systems. Stiffness in axial compression, anteroposterior and lateral bending was measured. On axial compression and lateral bending tests, URHEF was stiffer than DynaExtor(R) and Ilizarov systems. On anteroposterior bending test, URHEF was less stiff than 90degrees-50degrees configuration of the Ilizarov system but stiffer than 45degrees-135degrees configuration. Considering the fact that instead of 90degrees-90degrees transfixing pin fixatin system, 45degrees-135degrees configuration system is generally used in clinical practice, URHEF appears to be significantly stiffer than unilateral(DynaExtor(R)) and circular (Ilizarov)fixation systems. This mechanical study implicates that URHEF can be beneficially applied in the clinical use with assurance of mechanical stability.
External Fixators ; Olea

Multiple large bowel polyps are the hallmark of familial adenomatous polyposis (FAP), and many progress to colorectal cancer. Desmoid tumors are more common in patients with FAP than in other people, occurring, particularly, in those who have previously undergone prophylatic total colectomy. In such patients, desmoid tumors are a common cause of death. In an FAP patient without extracolic manifestation, who has undergone prophylatic surgery, multifocal desmoid tumors occur periodically. We report the serial radiologic findings of progressive desmoid tumors in FAP, drawing attention to the related findings of previous research.
Adenomatous Polyposis Coli ; Cause of Death ; Colectomy ; Colorectal Neoplasms ; Fibromatosis, Aggressive* ; Humans ; Polyps

PURPOSE: To evaluate the vascular pattern of lymph nodes, and the usefulness of color Doppler sonogaphy indifferentiating benign from malignant superficial lymphadenopathy. MATERIALS AND METHODS: Twenty-six patientswere pathologically and clinically confirmed to be suffering from benign reactive lymphadenitis and tuberculosis(n=16) or lymphoma and malignant lymphadenitis (n=10). Lymph node shape was assessed by the ratio of longitudinaldiameter to transverse diameter(L/T), and patients were thus assigned to one of two groups : L/T >or =2, or L/T<2.The hilar vascular pattern of lymph node was assessed by color Doppler sonography and classified as central,eccentric, or absent. On the basis of peripheral vascularity, patients were divided into three groups according tocircumferental linear vascularity. An absence of peripheral vascularity was classified as grade 0. If less thanhalf the periphery was covered by linear vascularity, a patient was assigned to as grade I, and if more than halfwas covered by a vessel, the classification was grade II. RESULTS: Statistically significant differences in L/Tratio were noted between malignant and benign node (p<.001). Of the 16 benign reactive nodes, 13 showed L/T >or =2,and 3 L/T<2. while in nine of the ten malignant nodes, L/T<2 was noted. Among 16 benign reactive nodes, hilarvascularity was central in 13, eccentric in one, and absent in two. Among the ten malignant nodes, thecorresponding totals were nil, four, and Six. The hilar vascular pattern showed statistically significantdifferentiation between malignant and benign node (p<.05). Among 16 benign reactive nodes, 13 were grade 0, twowere grade I, and one was grade II, while among ten malignant nodes, two were grade 0 and eight were grade I. Onthe basis of vascular pattern, the difference between benign and malignant nodes was statistically significant(p<.05). CONCLUSION: L/T ratio<2, absent or eccentric hilar vascularity, and the presence of peripheralvascularity are suggestive of malignant lymph node. The shape of LN and pattern revealed by an analysis of nodalvascularity using color Doppler sonography are useful in differential diagnosis of benign and malignantlymphadenopathy.
Classification ; Diagnosis, Differential ; Humans ; Lymph Nodes ; Lymphadenitis ; Lymphatic Diseases* ; Lymphoma

With the object of providing a temporary artificial periodonal ligament-like membrane around the dental implant, 10 Branemark type implants were coated with commercially available chitosan(Fluka Co., Buchs, Switzerland) which has a molecular weight of 70,000 and 80% deacetylation degree. Once this bioactive hydrophillic polymer(chitosan) contacts with blood or wound fluids, it becomes swollen and penetrates into the adjacent cancellous bone. Thus the interface between implant and surrounding bone is completely filled with chitosan. This tight junction in early healing phase enhances primary stability. The chitosan coated dental implants were implanted into the fresh patella bones from porcine knees, since the thickness of cortical bone is relatively even and their cancellous structure is homogenous. To test the shock absorbing effect, 1mm delta-rogette strain gage was installed behind the implant. The results showed 1. the principal strain peak value directed to the impact of coated implant was 0.064 0.018(p<0.05) and that of uncoated implant was 0.095(0.032 p<0.05). 2. the peak time delay of coated implant was 0.056sec(0.011 p<0.05) and that of uncoated implant was 0.024sec(0.009 p<0.05). It can be reasoned from this results that the chitosan coating has a shock absorbing effect comparable with a temporary artificial periodontal ligament.
Absorption* ; Chitosan* ; Dental Implants* ; Knee ; Membranes ; Molecular Weight ; Patella ; Periodontal Ligament ; Shock* ; Tight Junctions ; Wounds and Injuries

OBJECTIVE: It is still the important subject that 3D visualizing of medical images to help patient diagnose. There were many challenges for fast 3D visualization but it has some limitations that volume rendering without high price's hardware and software. Some techniques through the web were suggested to construct high quality's 3D visualization even though it was an only poor personal computer. This technique could share the volume rendering board and diagnose 3D images together. However there are some problems to construct web based 3D visualization. These are network delay, optimized visualization and security etc. The purpose of this paper was to visualize and control the 3D medical image having a high quality on the web. METHODS: To construct this system, we used mainly three tools which were VolumePro1000 board, WMV9(Windows Media Video 9 Codec) and socket functions based on TCP(Transmission Control Protocol). VolumePro1000 board could calculate quickly heavy matrixes of 3D images using phong's shading and shear-warp factorization. WMV9 was able to compress efficiently live images and to apply image streaming technique. Socket functions based on TCP transmitted messages to control the 3D images. RESULTS: We developed 3D visualization system and evaluated about image qualities and transmission conditions of different compression rates on unfixed network condition. CONCLUSION: It was big advantages that WMV9 encoder could decode automatically in many platforms(desk top, PDA, notebook, cellular phone, etc) without installing specific decoding programs if they only have Windows Media player. We expect 3D visualization system to be utilized various biomedical fields such as IIGS(Interactive Image Guided Surgery), CAD(Computer Aided Diagnosis) and Tele-medicine technologies.
Cellular Phone ; Humans ; Microcomputers ; Rivers*

No abstract available.
Female ; Humans ; Virilism*

No abstract available.
Cleft Lip* ; Prenatal Diagnosis* ; Ultrasonography*