1.Research Advance in Recombinant Expression of Microbial Lipases
China Biotechnology 2006;0(05):-
Microbial lipases have been widely used in traditional industries,as well as in emerging biocatalysed areas owing to their ability to catalyze a variety of reactions in aqueous and non-aqueous media.Therefore,it is very important to enhance amount of lipase production by recombinant overexpression for meeting market demand.A critical review of main and novel strategies which have been employed for recombinant expression of microbial lipases are presented,including codon optimization,fusion and co-expression,dual expression system based on hybrid promoters,homologous overexpression,cell surface displaying and high-throughput screening based on gene library of expression.These new technologies are gradually coming to the forefront in the recombinant expression of lipase,especially for cell surface displaying and high-throughput screening based on gene library of expression.Meanwhile,several recombinant expressions for representative microbial lipases were also introduced and discussed,which are available for consultation when attempting to overexpress any lipases by scientists and industrialists.
2.A clinical analysis of rectal prolapse treated by presacralrectopexy.
Kwang Yun KIM ; Jun Ho SHIN ; Yong Shin KIM
Journal of the Korean Society of Coloproctology 1991;7(1):45-49
No abstract available.
Rectal Prolapse*
3.Optimization of Lipase Production Conditions by Geotrichum candidum Y162 Using Single Factor-response Surface Methodology
Jin-Yong YAN ; Jiang-Ke YANG ; Yun-Jun YAN ;
China Biotechnology 2006;0(08):-
The fermentation conditions of lipase production by Geotrichum candidum Y162 were optimized. Initially, the most suitable carbon olive oil, nitrogen source soybean flour and NH4Cl, salt BaCl2 and MgCl2 were selected according to single factorial experiments respectively. Based on the result, screening methodology Plackett-Burman design was used to evaluate the effects of twelve factors related to lipase production and three statistically significant factors olive oil, BaCl2 and NH4Cl were selected. The path of steepest ascent was used to approach the optimal region of lipase production subsequently. Then, the optimal combined concentration for maximum enzyme activity were further optimized by response surface methodology and determined as follows: olive oil 2.35%, BaCl2 0.36%,and NH4Cl 4.69%.The optimization of culture conditions of G.candidum Y162 led to a 2.25-fold increase in lipase production relative to initial result 14.16 U/ml, which indicate that single factor in combination with response surface methodology is an effective method for optimization of lipase production conditions by G.candidum Y162.
4.The effect of recombinant human growth hormone on the expression of tumor necrosis factor in the rat model of obstructive jaundice
Jun SONG ; Jingang LIU ; Yong WANG ; Yun YU
Journal of Chinese Physician 2000;0(12):-
Objective To study the effect of recombinant human growth hormone(rhgh) on the expressions of tumor necrosis factor a(TNFa) and endotoxemia in murine experimental obstructive jaundice(OJ) models.Methods Sixty adult male wistar rats.were randomly divided into three groups A: sham operation group(SO),20;B:OJ group,20;C: OJ+rhGH group,20.The rats in group OJ and OJ+rhGH suffered from bile duct ligation,while the SO group did not.Two weeks later,rats in OJ+rhGH group were injected with rhGH subcutaneously once a day.The dose was 0.75 Iu/kg.The other two groups were injected with the same amount of saline.The endotoxin(ETX),the liver function and TNFa were detected four weeks later.Result The level of ETX(0.036?0.010EU/m1) and TNFa(182.00?88.37lpg/ml) in OJ+rhGH were much lower than those in OJ group(0.065?0.011EU/m1 P
5.A case report of adenoid cystic carcinoma on maxillary antrum and infratemporal space.
Do Geun JANG ; Yong Gyoo LEE ; Sa Yub KIM ; Jun Yun KIM ; Jin Soo KIM
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1993;19(4):583-588
No abstract available.
Adenoids*
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Carcinoma, Adenoid Cystic*
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Maxillary Sinus*
6.A Case of Nevus Lipomatosus Cutaneous Superficialis.
See Yong PARK ; Choong Rim HAW ; Soo Nam KIM ; Byung Jun KIM ; Tae Yun YOO
Korean Journal of Dermatology 1977;15(3):353-357
Nevus lipomatosus cutaneous superficialis is a very rare skin disease, which usually is present at birth. The lesion shows groups of asymptomatic soft papules or nodules, which have a smooth or folded surface and are skin colored or yellowish. The area of predilection is the pelvic girdle (especially the buttock and sacrococcygeal areas). Microscopically, nevus lipomatosus cutaneous superficialis reveals groups of etopic fat cell nests within the dermis. Only one case was reported in Korea(1969). The authors observed a case of typical nevus lipomatosus cutaneous superficialis. The patient was 19 year-old male, who ha,ve had asymptomatic soft papules and nodules on the buttock, lower back and scalp since 14 years of his age. Routine laboratory findings were within normal limit. Histopathological findings showed etopic fat cell nests within mid and lower dermis (Fig. 3) Diagnosis was confirmed by clinical and histopathological findings. Litercature was briefly reviewed for the discussion.
Adipocytes
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Buttocks
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Dermis
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Diagnosis
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Humans
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Male
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Nevus*
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Parturition
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Scalp
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Skin
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Skin Diseases
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Young Adult
8.Anti-dementia effect of Tongluo Xingnao effervescent tablet based on urinary metabonomics.
Jiang-ping WEI ; Yin-jie ZHANG ; Yun-tong MA ; Shi-jun XU ; Yong-yan WANG
China Journal of Chinese Materia Medica 2015;40(16):3287-3292
Tongluo Xingnao effervescent tablet (TLXNET) is a patented prescription, which comes from modified Xionggui decoction and can improve cognitive function. However, its effect on the urine metabolites and anti-dementia mechanism in the dementia model rats induced by hippocampal injection with Aβ25-35 remains unclear. The experiment focused on the changes in trajectory and inter-relationship among the urinary metabolite of rats in the blank group, Aβ25-35 hippocampal injection dementia model group and the TLXNET intervention group, in order to determine theirs characteristic metabolic markers and explain the anti-dementia effect of TLX-NET base on the change of metabolic trajectory of these bio-markers. According to the experimental results, 5, 6-indolequinone, 4-hydroxyphenyl pyruvic acid (4-HPPA), cortisol and 3-thiosulfate lactic were preliminarily identified as the characteristic metabolic markers. They mainly participate in dopamine system, glucocorticoids and energy metabolic pathways. TLXNET can apparently downregulate the disturbances of metabolic trajectory of the four bio-markers. The experiment indicates that the dementia model induced by injecting Aβ25-3 into hippocampus has its characteristic endogenous metabolic markers in urine, and ELXNET can ameliorate dementia by down-regulating the disturbances of metabolic trajectory.
Amyloid beta-Peptides
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metabolism
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toxicity
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Animals
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Biomarkers
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urine
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Dementia
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drug therapy
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urine
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Drugs, Chinese Herbal
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administration & dosage
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Female
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Hippocampus
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drug effects
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metabolism
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Humans
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Male
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Metabolomics
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Peptide Fragments
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metabolism
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toxicity
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Rats
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Rats, Sprague-Dawley
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Tablets
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administration & dosage
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Urine
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chemistry
10.Clinical Study on Wuteng Ercao Decoction in Treating Active Rheumatoid Arthritis
Yong LUO ; Yonghe HU ; Mingyang GUO ; Min YANG ; Jun ZHANG ; Mingdong YUN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(9):33-35
Objective To observe the efficacy of Wuteng Ercao decoction in treating active rheumatoid arthritis (RA) and its effects on the levels of cytokine interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).Methods Sixty-five patients with active RA were randomly divided into treatment group (33 cases) and control group (32 cases). Patients in the control group were administered with methotrexate tablets 10 mg, oral taking, once a week;hydroxychloroquine sulfate tablets 200 mg, oral taking, twice a day;diclofenac sodium sustained release tablets 75 mg, oral taking, twice a day. Patients in the treatment group were administered with Wuteng Ercao decoction additionally, oral taking, three times a day. The treatment lasted for 12 weeks. The changes and untoward effects of various clinical and laboratory indicators were observed before and after treatment.Results After treatment, the tender joint count, swollen joint count, patient’s global assessment, and physician’s global assessment were improved (P<0.05), with statistical significance between the two groups (P<0.05). The levels of ESR, CRP, IL-6 and TNF-α decreased significantly after treatment (P<0.05), but those were more significantly in treatment group (P<0.05). There were 2 cases with untoward effects in the treatment group and 1 case in the control group (P>0.05).Conclusion Wuteng Ercao decoction is effective in patients with active RA and it can reduce the levels of IL-6 and TNF-α.