PURPOSE: The aquaporins (AQPs) are transmembrane water channel proteins. It is well known that AQP2, -3 and -4 contribute to the urinary concentration in collecting duct (CD) and also reported the presence of these three AQPs in the connecting tubule (CNT). Newborn rats are not capable of producing a concentrated urine. Rats develop the ability to concentrate urine after birth. The purpose of this study was to establish the time of the expression and the distribution of AQP2, -3 and -4 in the developing rat kidney. MATERIALS AND METHODS: Sprague-Dawley rats were used in all experiments. Kidneys were obtained from 16, 18 and 20-day-old fetuses and 1, 4, 7, 14 and 21-day-old pups and preserved and processed for immunohistochemical studies using a preembedding immunoperoxidase procedure. AQP2, -3 and -4 immunoreactivity was detected using rabbit polyclonal antibody and donkey anti-rabbit IgG. RESULTS: AQP2, -3 and -4 appeared first in 16-day-old fetuses in the CD and in 18-day-old fetuses in the CNT. Immunoreactivity for AQP2, -3 and -4 was markedly increased after birth and gradually increased during development. In CNT cells and principal cells, AQP2, -3 and -4 were not distinctly demonstrated on the apical, lateral and basal plasma membrane respectively until 21 days after birth. Distinct polarity of these AQPs both in CNTcells and principal cells were observed at 21 days after birth. CONCLUSIONS: AQP2 -3, and -4 were expressed not only in CD but also in CNT before developing of urine concentrating ability during development and it is concluded that their expression and distribution in CNT may play a role in the development of urine concentration abilities in rat kidney.
Animals ; Aquaporin 2* ; Aquaporins ; Attention ; Cell Membrane ; Equidae ; Fetus ; Humans ; Immunoglobulin G ; Infant, Newborn ; Kidney Concentrating Ability ; Kidney Tubules ; Kidney* ; Parturition ; Rats* ; Rats, Sprague-Dawley

PURPOSE: We retrospectively reviewed the cases of ureteroscopic stone removal at our hospital and defined the efficacy, safety and the skilled experience of the surgeon. MATERIALS AND METHODS: Ureteroscopic stone removal was done in 510 cases. Sixty eight stones were located in the upper ureter, 114 stones in the mid ureter, and 328 stones in the lower ureter. The stone size was less than 5mm in 210 cases, from 5 to 10mm in 241 cases and more than 10mm in 59 cases. The objective cases were categorized into three groups according to the experience of the surgeon, and then compared to each group. RESULTS: The overall success rate was 94.5%. The success rates of upper, mid and lower ureteral stones were 86.7, 90, and 96.9%. The success rate was 95.7% in stones less than 5mm, 94.1% in 5 to 10mm and 91.5% in more than 10mm. The operation results were improved and stabilized after 50 cases. There were 48 cases (9.4%) of complications which were treated successfully with conservative treatment except for 2 cases. CONCLUSIONS: Our study suggests that the ureteroscopic stone removal technique of the surgeon is improving by accumulating operation experience, and stabilized skillful technique will be achieved after experience of more than 50 cases.
Retrospective Studies ; Ureter ; Ureteroscopy

Background: Similar to smoking, exposure to secondhand smoke is a risk factor for developing hypertension and cardiovascular diseases; however, there is no standardized method for measuring smoke exposure. Measuring urine cotinine levels is one possible means for determining the degree of exposure to secondhand smoke. This study investigated the association between urinary cotinine levels and blood pressure in Korean adults exposed to secondhand smoke. Methods: Data from the Korea National Health and Nutrition Examination Survey conducted between 2016 and 2018 were used. A total of 9,273 participants aged ≥19 years self-reported as current non-smokers, which was cotinine- verified. A complex sample general linear model regression analysis was performed to analyze the association between urine cotinine and blood pressure. A P-value of <0.05 was considered statistically significant. Results: Corrected urine cotinine levels were positively associated with systolic and diastolic blood pressure in female participants (P<0.001 and P=0.040, respectively). Furthermore, a 10-fold increase in the corrected urine cotinine level of those in contact with secondhand smoke was independently associated with 2.085 mm Hg and 0.575 mm Hg increases in systolic and diastolic blood pressure, respectively. However, there was no association between systolic and diastolic blood pressure in male participants (P=0.226 and P=0.256, respectively). Conclusion Urinary cotinine levels were positively associated with increased blood pressure in females exposed to secondhand smoke. Therefore, urinary cotinine may be used as an indicator to quantify and monitor the effects of blood pressure elevation in females exposed to secondhand smoke.

The authors report eight cases of thoracic stenosis due to ossification of ligamentum flavum. Motor and sensory deficits were found in all cases and urinary incontinence was found in one case. Diagnosis was made from simple x-rays, myelography, computerized tomography, myelography-CT, and magnetic resonance imaging. Treatment consisted of sufficient posterior decompressive laminectomy and medial facetectomy which resulted in satisfactory improvement in 6 cases, fair course in 1 case and poor course in 1 case.
Constriction, Pathologic* ; Diagnosis ; Laminectomy ; Ligamentum Flavum* ; Magnetic Resonance Imaging ; Myelography ; Urinary Incontinence

No abstract available.
Poroma*

Spinal stenosis has several types of etiology such as degenerative, spondylolisthetic and postoperative, etc. Operative management for spinal stenosis is adequate decompression and stabilization of the unstable lumbar spine created by the decompressive procedure. We analysed 52 operative cases of spinal stenosis delete from January 1994 to October 1996. The following results were obtained 1) The male and female ratio was 1:1.9 and mean age was 52.1 years old 2) Mean follow-up period was 20.3 months 3) The involved site was one level in(28)(53.9%), two level in(19)(36.5%), and three levels in(5)(9.6%) 4) For decompression method, total laminectomy combined with foraminotomy and facetectomy was employed procedure. 5) For stabilization, bilateral posterolateral fusion was performed in major damaged facet joint and transverse process. Instrumentation was combined in 61.5% delete of all cases 6) According to the criteria of Kirkaldy-willis, excellent and good results were shown in 90.6% of fused group with instrumentation and 85% of fused group without instrumentation.
Decompression ; Female ; Follow-Up Studies ; Foraminotomy ; Humans ; Laminectomy ; Male ; Spinal Stenosis* ; Spine ; Zygapophyseal Joint

BACKGROUND: Decision about life sustaining treatments ought to be based on the patient's informed preferences. This study was to see if there were any differences in acceptance by patients, their primary care givers and doctors for end-of-life care according to situations, and if any, to analyse the factors related with different attitudes. METHODS: A structured questionnaire survey of end-of-life care preferences was performed on 162 cancer patients and their primary care givers in four university hospitals and one general hospital from March 1, 1999 to February 29, 2000. A similar survey was done for doctors practicing at the above hospitals during the same period to investigate their attitudes toward providing end-of-life care to an assumed nearly bed-ridden patients. ANOVA, t-test and Wilcoxon rank sum test were used to compare acceptance of intervention among the groups or according to the various situations. Factors presumed to be related to the acceptance were sought and analysed by stepwise multiple regression. RESULTS: The difference in acceptance of intervention between the primary care giver group and the doctor group was not significant in almost every situation, showing significantly higher than the patient group (P<0.001). All three groups showed higher acceptance when a therapeutic intervention rather than a diagnostic test was proposed (P<0.001), when expected survival was 30 days rather than 7 (P<0.01), and when the therapeutic intervention was thought as non-invasive rather than invasive (P<0.001). The less anxious the patient was, the higher the acceptance from the patient. Patients with a religion had higher acceptance rate than non-religious patients. Primary care givers who expected cure of the disease accepted more of the postulated care than those who did not (P<0.05). Wives or mother-in-laws of patients showed lower acceptance than those in other relationship (P<0.05). The longer the patient had been diagnosed with cancer, the higher the acceptance of the primary care giver (P<0.1). Direct relatives showed higher acceptance than that of collaterals (P<0.1). Family doctors specializing in family medicine had lower acceptance than doctors of other specialties and interns (P<0.05). CONCLUSION: The acceptance of intervention by patients was lower than that of primary care givers and doctors and depended on the expected survival and the type of intervention.
Attitude to Death ; Diagnostic Tests, Routine ; Hospitals, General ; Hospitals, University ; Humans ; Palliative Care ; Primary Health Care* ; Spouses ; Surveys and Questionnaires

Neuroglialchoristoma is a rare cerebral heterotopia typically involving extracranial midline structures of the head and neck, including the nose, nasopharynx and oral cavity. It rarely involves non-midline structures, such as the middle ear, mastoid and orbit. We report the case of a 63-year-old woman with right-sided hearing loss and aural fullness who was diagnosed with neuroglialchoristoma of the middle ear and mastoid. To our knowledge, this is the first report on neuroglialchoristomawith massive tympanosclerosis. The presence of combination supported the inhalation theory of neuroglialchoristoma, given that tympanosclerosis is typically caused by Eustachian tube dysfunction.
Choristoma* ; Ear, Middle* ; Encephalocele ; Eustachian Tube ; Female ; Head ; Hearing Loss ; Humans ; Inhalation ; Mastoid ; Middle Aged ; Mouth ; Myringosclerosis* ; Nasopharynx ; Neck ; Neuroglia ; Nose ; Orbit

BACKGROUND AND OBJECTIVES: Auditory temporal resolution, which refers to the time-related aspects of acoustic processing, can be evaluated by the Gaps-In-Noise (GIN) test. We investigated whether the presence of high frequency sensorineural hearing loss (HF-SNHL) affects the temporal resolution of GIN performance in older adults with normal hearing. SUBJECTS AND METHOD: Hearing tests including the GIN test were performed in 87 subjects with normal pure tone average. The GIN threshold and percentage of correct answers were compared among 4 groups of participants; older adults with normal hearing (n=18), older adults with HF-SNHL (n=24), young adults with normal hearing (n=24) and young adults with HF-SNHL (n=21). RESULTS: There was no significant difference in the mean GIN thresholds between the HF-SNHL group (5.8+/-0.8 msec) and the normal hearing group (6.0+/-0.8 msec) in older adults, whereas the mean GIN thresholds of HF-SNHL group was higher than that of the normal group in young adults (4.6+/-0.3 msec vs. 4.2+/-0.5 msec, p<0.05). The mean percentage of correct answers of HF-SNHL group (62.5+/-5.5%) was not significantly different from that of the normal hearing group (60.6+/-3.9%) in the old, unlike in the young (71.3+/-4.0% with HF-SNHL vs. 76.9+/-4.3% with normal hearing, p<0.05). Age only showed a significant correlation with the GIN performance. Neither the GIN threshold nor the GIN perception level had any relation with the presence of HF-SNHL in older adults. CONCLUSION: We found no evidence that supported the influence of HF-SNHL on auditory temporal resolution in older adults. These results imply that HF-SNHL may be of little importance in gap detection after age-related changes in central auditory system.
Acoustics ; Adult ; Hearing Loss, High-Frequency ; Hearing Loss, Sensorineural* ; Hearing Tests ; Hearing* ; Humans ; Young Adult*

BACKGROUND AND OBJECTIVES: This study aimed to analyze the prevalence of superior semicircular canal dehiscence (SSCD) in the coronal images of high-resolution temporal bone computed tomography (TBCT) and to evaluate the diagnostic accuracy of coronal images for SSCD syndrome. SUBJECTS AND METHOD: We retrospectively reviewed high-resolution TBCT scans of 217 patients (434 ears) with SSCD due to various causes. The dehiscence ratio (slices showing dehiscence/total slices showing the superior semicircular canal) in the coronal images of TBCT was calculated, and the optimal cutoff value for the diagnosis of SSCD syndrome was determined using the receiver operating characteristics (ROC) curve. RESULTS: Of the 434 ears, 64 (14.7%) presented SSCD in more than one slice of the coronal images of TBCT, but only three patients (0.7%) were confirmed with SSCD syndrome. Based on the ROC curve analysis for the dehiscence ratio of 64 ears, the optimal cutoff value for the diagnosis of SSCD syndrome was 0.67 with 100% sensitivity and 90.2% specificity. CONCLUSION: The majority of cases diagnosed with SSCD syndrome using the coronal images of TBCT were asymptomatic or false-positive. The dehiscence ratio in the coronal images of TBCT combined with a typical symptom can be a highly sensitive and specific diagnostic tool for SSCD syndrome.
Diagnosis ; Ear ; Humans ; Methods ; Prevalence* ; Retrospective Studies ; ROC Curve ; Semicircular Canals* ; Sensitivity and Specificity ; Temporal Bone*