1.Endocrine Change during Menopausal Transition.
Journal of the Korean Medical Association 2000;43(5):396-403
No abstract available.
2.Production and Characterization of Monoclonal Antibodies to a 75-kDa Major Outer Membrane Protein of Porphyromonas gingivalis.
Journal of the Korean Society for Microbiology 1997;32(6):633-644
Porphyromonas gingivalis has been implicated in adult periodontitis. A 75-kDa major outer membrane protein of P. gingivalis is an immunodominant antigen and is often associated with fimbrial proteins during its purification. The present study was performed to generate monoclonal antibodies (MAbs) to the 75-kDa protein and characterize the protein and the MAbs. Kight-week-old BALB/c mice were immunized with the partially purified 75-kDa protein from P. gingivalis 2561. Spleen cells of the mice were hybridized with SP2/0 Ag-14 mouse myeloma cells and then fused with polyethylene glyeol 3400. Hybrid cells surviving hypoxanthine/aminopterin/thymidine medium were tested for antibody secretion by ELISA. Positive clones were subcloned by limiting dilution and then three subclones were obtained. The subclones were injected peritoneally into Pristane-primed mice to produce MAbs. Immunoreactivity of the MAbs was confirmed by an immunoblot analysis. In the immunoblot using a partially denatured crude 75-kDa protein preparation as an antigen, the 75-kDa protein reacting with the MAbs revealed a ladder- like pattern which has been known as a characteristic of a native polymeric form of fimbriae. The intervals of the ladders, however, were different from those of fimbrial protein. The MAbs also recognized the completely denatured 75-kDa protein. Immunogold localization using IgG fraction of the MAbs demonstrated that the gold particles were deposited on globules on the surface of P. gingivalis 2561, but not on fimbriae. These results suggest that the 75-kDa protein is antigenically different from fimbrial protein and in nature, is not associated with, but may be physicochemically associated with the fimbrial protein during extraction and purification procedures for these proteins. Immunoreactivity of these 3 MAbs was tested by immunoblot using sonic extracts from various P. gingivalis strains. All the MAbs reacted only with the strains having proteins in their sonic extracts with a molecular mass of 75 kDa, but failed to recognize the strains having proteins with molecular masses of 61, 61.5, 76, and 78 kDa. These results indicate that strains of P. gingivalis have antigenically different major outer membrane proteins and the MAbs in the present study might have been raised against the same common, exposed immunodominant epitope in the 75-kDa proteins.
Animals
;
Antibodies, Monoclonal*
;
Chronic Periodontitis
;
Clone Cells
;
Enzyme-Linked Immunosorbent Assay
;
Hybrid Cells
;
Immunoglobulin G
;
Membrane Proteins*
;
Membranes*
;
Mice
;
Polyethylene
;
Polymers
;
Porphyromonas gingivalis*
;
Porphyromonas*
;
Spleen
5.Modified fontan procedure with extracardiac edicardial lateral tunnel: New surgical technique.
The Korean Journal of Thoracic and Cardiovascular Surgery 1993;26(5):422-425
No abstract available.
Fontan Procedure*
6.Clinical trial of myocardial protection using cold oxygenated diluted blood cardioplegia in child age.
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(3):211-219
No abstract available.
Child*
;
Heart Arrest, Induced*
;
Humans
;
Oxygen*
7.Effect of pituitary transplantation and haloperidol administration on hypothalamic LHRH release in female rats.
Byoung Moon KANG ; Jin Yong LEE
Korean Journal of Obstetrics and Gynecology 1991;34(1):42-52
No abstract available.
Animals
;
Female*
;
Gonadotropin-Releasing Hormone*
;
Haloperidol*
;
Humans
;
Rats*
10.Exposed level of workers in the factory next to a led recycling factory.
Jin Ha KIM ; Duk Hee LEE ; Yong Hwan LEE
Korean Journal of Preventive Medicine 1996;29(3):693-700
The purpose of this study was to determine whether workers at a factory next to a lead recycling factory in Pusan, were affected by lead contamination. The mean air lead concentration of lead recycling factory was 0.21mg/m3(TWA=0.05mg/m3). Thirty-nine male workers of Factory A, Cr. plating factory next to the lead recycling factory were exposed group and a comparison group, 62 male workers of Factory B were selected from another Cr. plating factory about 8.5km away from lead recycling factory. Air lead concentration of each workplace was checked for 4 times from August 5 to August 20 in 1995 by low volume air sampler. Each subject was interviewed about age, life-style, smoking, work history, and residence etc, and venous blood was drawn for lead measurement by graphite furnace atomic absorption spectrometry. We have observed that air lead concentration and blood lead concentration of Factory A was higher than Factory B(2.6 +/- 1.6 Vs. 1.2 +/- 0.2 microgram/m3, 14.9 +/- 1.6 Vs. 12.2 +/- 1.6 microgram/dl). We believe that other environmental lead sources such as transportation and residence did not affect air lead and blood lead concentration differences of both factory. We concluded that high air lead and blood lead concentration of Factory A were caused by lead contamination generated by the neighboring lead recycling factory.
Absorption
;
Busan
;
Graphite
;
Humans
;
Male
;
Recycling*
;
Smoke
;
Smoking
;
Spectrum Analysis
;
Transportation