Objective:To explore the participation mode of clinical pharmacists in anti-infective therapy. Methods:Clinical phar-macists provided rational medication suggestions for anti-bacterial and antifungal therapy, and carried out pharmaceutical care for one patient with lung and intracranial infection after decompressive craniotomy for severe brain injury. Results: According to the sugges-tions of clinical pharmacists, fluconazole therapeutic regimen and amphotericin B therapeutic regimen was applied in turn respectively with the treatment course of 32d and 28d. During the treatment, the pharmacists also provided therapy advice on the infection induced by Staphylococcus epidermidis and Pseudomonas stutzeri, ADR warning and monitoring of fluconazole and amphotericin B, and adjust-ment of anti-infective therapy according to the results of antimicrobial susceptibility testing. The therapeutic effect was significant. Conclusion:The participation of clinical pharmacists in drug treatment can optimize medical therapy, which contributes to the coopera-tion between doctors and pharmacists.

In plant genetic engineering, selectable marker is needed to distinguish transformant. As the commercialization of transgenic plants, people are more and more paying close attention to their safety, among which mainly refers to the safety of selectable marker. In order to increase the safety of transgenic plants, biologists began to search for biosafe selectable marker.Current research progress of taking key enzyme in metabolic pathways were overviewed,which include glycometabolism, amino acid metabolism, hormone metabolism ,nucleotide metabolism and protein metabolism et cetera, as selectable marker in transgenic plants.

Humans ; Olfaction Disorders ; genetics ; Smell ; genetics

Glycoprotein N is encoded by glycoprotein N (gN) gene of herpesviruses. The amino acid composition and expression level of this protein vary among difference species of herpesviruses. According to present studies, gN protein is expressed in cytoplasm of host cells, mainly in endoplasmic reticulum. The gN forms a complex with glycoprotein M in host cells. The complex is involved in the processes of viral replication and inter-cellular infection. Moreover, this protein plays a role in immune evasion from host immune system. The study will provide a theoretical basis for further study of herpesvirus gN gene and its encoded protein.
Animals ; Herpesviridae ; genetics ; metabolism ; Herpesviridae Infections ; virology ; Humans ; Viral Envelope Proteins ; genetics ; metabolism

Adult ; Diagnostic Errors ; Female ; Humans ; Middle Aged ; Myasthenia Gravis ; diagnosis

Genetic Predisposition to Disease ; Humans ; Olfaction Disorders ; congenital ; genetics

Objective: To evaluate the reliability of the digital marker tracing method combined with center-of-mass algorithm in measuring human pelvic displacement. Methods: Six cadaveric specimens of the third lumbar vertebra to the proximal 1/3 part of femur were used in present study. The specimens had no structural abnormality and all the soft tissues were dissected, reserving the hip joint capsules and the ligaments of the pelvic ring and floor. Markers with black dots against white back-ground were used to mark the key points of the pelvis. Axial loadings from the proximal lumbar (simulating the two-legged stance) were applied by MTS in the gradient of 0 N to 500 N. Images of the front and lateral views were obtained using two CCD cameras. Using Image J software, we calculated the vertical displacement of S1, (the first sacral vertebrae) in the front view and the micro-angular rotation of sacroiliac joint in the lateral view by measuring the marker's movement. Results: There was good correlation between the marked points before and after deformation of the pelvis, with the average correlation coefficient being 0. 983. Based on the 768 X 576 pixels, pixel size (mm) 0. 681 5 X 0. 681 5 image,the accuracy of the displacement was about 0. 018 pixels and the comparative error could reach 1. 11‰. The load-displacement curves obtained in this study accorded well with the clinical results. The pelvic load-displacement curve exhibited approximately a linear behavior; the sacroiliac joint load-angular rotation curve in the sagittal plane exhibited a non-linear behavior. Under a vertical load of 500 N,the average vertical displacement of S1, of the pelvis was (0. 835 6 ± 0. 283 0) mm and the average micro-angular rotation of sacroiliac joint in lateral view was (0. 584 ±0. 221). Conclusion: Digital marker tracing method combined with the center-of-mass algorithm is a simple, accurate method for measurement of pelvic displacement, which can be widely used in biomechanical research.

AlM:To explore the suitable conditions in rapid model of corneal neovascularization ( CNV ) after thermal burn under different constant temperature in rabbit.
METHODS: Total 45 New Zealand white rabbits were divided randomly into five groups ( A, B, C, D, E ) . A groups:100℃ ( n = 10 ) , B groups: 200℃ ( n = 10 ) , C groups:300℃ ( n=10 ) , D groups: 400℃ ( n=10 ) , and E groups:control group ( n=5 ) . All left eyes of rabbits in A,B,C,D groups were induced corneal neovascularization by constant temperature burning device. The growth of CNV was observed by slit lamp microscope and the area of CNV were recorded on 4 th , 7 th , 14 th , 30 th days postoperatively. SPSS 19. 0 statistical package was used for data analysis, and the data was recorded by mean ± standard deviation. Comparison by analysis of variance was made by repeated measures in the area of neovascularization at each time point in groups. Statistical tests were considered significantly when P values were less than 0. 05.
RESULTS:On postoperative 4 th , 7 th , 14 th , 30 th days: no neovascularization was found after corneal thermal burn in A group, but only a few nebula left (n=2);the area of CNV were (9.16±1.45)mm2, (37.73±5.49)mm2, (62.44± 7. 54 ) mm2 , ( 40. 28 ± 7. 39 ) mm2 in B group respectively;and (11.45±1.04)mm2, (44.51±4.64)mm2, (66.13±4.13)mm2, (43.04±2.33)mm2 in C group respectively; and (13.23± 0.86)mm2,(47.26±4.59)mm2,(67.57±4.56)mm2,(45.59± 4. 44 ) mm2 in D group respectively, and part corneal carbide ( n = 4 ) was observed as well as corneal perforation ( n= 6 ) were found on 3d in D group. No neovascularization was found in normal control group. Comparison of the areas of CNV at each time point between groups was statistically different, P < 0. 05. Statistical differences were found among B, C, D groups, P<0. 05.
COCLUSlON:ln 4 to 7d, the higher the temperature is, the more the neovascularization area of CNV are. lt has no significant difference in 14 to 30d. But corneal carbide and corneal perforation are often found in 400℃ group, so its modeling failure rate is high. lt is between 200℃ and 300℃ that repeatability and uniformity of the corneal neovascularization model of rabbit are superior.

AlM: To observe the treatment efficacy of eyelid herpeszoster with external use ganciclovir ophthalmic gel.METHODS:A total of 77 patients were randomly divided into treatment group and control group. ln the treatment group, ganciclovir ophthalmic gel was topical coated on the basis of antiviral drugs, while the control group was coated with conventional acyclovir ointment and antiviral treatment.
RESULTS: The cure rate was 95% in the treatment group, and the control group was 83% after 10d treatment. The difference was significant.
CONCLUSlON: The efficacy of ganciclovir ophthalmic gel treated eyelid herpes zoster is obvious.

The rodipicolinate synthase gene was cloned from Nicotiana tabacum L.cv,which encodes the key enzyme in the synthesis of lysine,and then a vitro molecular reform reliefing feedback suppression was made. Some resistant seedlings were acquired using the mutated gene as selectable marker and analog of lysine as selectable reagent,and the detection results were positive by means of PCR and Real-Time PCR,however the phenotype is somewhat abnormal.