Bacterial disease is still the important disease hazardous to health of human being and animals.The bacterial mutation technique is an important bacterial research technique,including traditional physical and chemical and biological method,and modern genetic mutation technique.The bacterial genetic mutation technique is one of the research focus in bacteriology nowadays,the mutation strategy varies with different kinds of genetic:mutation technique.The development and application of bacterial mutation technique offers new tools for research on new bacterial vaccine,bacterial genetic function and genetic therapy etc.

The concept and characteristics of omnimedia were outlined, the effects of omnimedia on traditional in-formation service model, storage types of information resources, and personal service in medical library were analyzed, and a number of new information service models in the omnimedia era were put forward.

An innovative educational model characterized as interesting-guiding is created in the acupuncture educational practice for foreign students. According to the innovative educational theories, teaching orders are rearranged, new teaching methods are used, and passive indoctrination is changed into creative studies and discussion, thus the educational quality is improved.

Hepatic cancer is currently the fifth most common malignant neoplasm in the world.Surgical resection is considered as radical treatment.Patients with hepatic cancer in middle or advanced stage according to the Barcelona clinic liver cancer staging system (BCLC) are usually with huge and (or) multinodular lesions and vascular invasion,which are not generally recommended for surgical resection because of high operative mortality,recurrence rate and dismal survival benefit.However,many centers have proved an opposite and encouraging result against the opinions above.With the development of surgical techniques and intensive medical care,the concern of high postoperative mortality for middle or advanced stage hepatic cancer patients is no longer unsolvable.Precise preoperative assessment is essential.The estimation of the liver functional reserve has developed from simple Child-Pugh score to an integrated system including computed tomography evaluation,indocyanine green clearance test,hepatic venous pressure gradient,etc.The estimation of the remnant liver volume after hepatectomy is especially important for surgical treatment for the middle or advanced stage hepatic cancer.Insufficient liver remnant was absolute contraindication for major hepatectomy because of high incidence of postoperative liver failure.In-situ liver transection with one branch of the portal vein ligation has been invented as a novel method to stimulate fast liver regeneration; by this way,a second-stepped radical resection can be performed with a plenty of liver remnant one week later.However,the reliability for hepatic cancer patients with liver cirrhosis is still unknown.Meticulous surgical procedure is another key factor for a safe major hepatectomy.Radical resection is most expected to provide better survival.The development of the technique of liver blood flow occlusion has a markedly influence on partial hepatectomy.Highly selective occlusion and even occlusion-free hepatectomy can reduce warm ischemia injury and improve postoperative survival.Anterior approach is a reasonable maneuver for huge hepatic cancer resection.Cancer thrombosis usually indicates poor prognosis,however,if en-bloc resection or separate thrombectomy can be achieved,surgical resection for the middle and advanced stage hepatic cancer still can provide a better survival benefit to this category of patient than palliative treatment as TACE.

Objective To carry out the performance evaluation to insure security of inner canister during the design phase, which is the important thin component of the portable sterilizater. Methods The software Mechanica, one of the module of Pro/Engineer was implemented to the flexure analysis of the inner canister. Results The stabilization and strength of inner canister satisfied requirements of the design. Conclusion Not only the security of design data can be proved but the cost and cycle of design can be reduced by using the method of expansion simulation analysis at the same time of the design phase of the equipment.

As the efficacies of chemotherapy in the treatment of advanced non-small cell lung cancer(NSCLC) have reached a plateau, the molecularly targeted therapy becomes a new method to improve curative effects.The molecular targeted drugs include epidermal growth factor receptor inhibitors, anti-angiogenesis drugs, etc.As one of the representative drugs of EGFR inhibitors, Gefitinib, has a low clinical benefit index.Erlotinib, the first EGFRTK inhibitor, can enhance the survival of patients, and may serve as a potential choice for the first-line treatment of advanced NSCLC.Bevacizumab combined with chemotherapy has shown a favorable clinical effect in the first-line treatment, and it is also the first anti-angiogenesis drug approved by the FDA that can be used in cancer treatment.These drugs, together with clinical and experimental progress in many other targeted drugs, have shown a favorable prospect for targeted drugs in the treatment of advanced non-small cell lung cancer.

Objective To compare the early efficacy of two different ways of rehabilitation exercises in total knee arthroplasty (TKA) patients. Method From January 2011 to January 2014,136 patients having undergone TKA were randomly divided into two groups. In group A , patients exercised under the guidance with doctor. In group B, patients exercised by using continuous passive movement (CPM) machine. Compared the data from the two groups including range of motion (ROM) of the knee joint, knee function score (KFS) and circumference of the knee joint, t test of independent sample was utilized in the research. Results All patientswere followed up in 4 weeks. The ROM and KFS of Group A was significantly better than Group B (P < 0.05). The knee circumference had no statistically significant difference between two groups (P> 0.05).Conclusion Group A and B are effective methods in TKA patients in early rehabilitation training. But training under the guidance of doctor has greater advantages than CPM.

Objective To evaluate the safety and feasibility of hysteroscopy in the treatment of endogenous type cesarean section scar pregnancy (CSP). Methods Clinical data of 58 cases of CSP from August 2012 to February 2014 in our hospital were analyzed retrospectively.Thirty-one cases were treated by hysteroscopy,while 27 cases were treated by laparoscopy.The intraoperative blood loss,operation time,postoperative hospital stay,postoperative drainage of uterine cavity,β-hCG level resolution time and time to menstruous restoration were compared between the two groups. Results The intraoperative blood loss,operation time,length of hospitalization after operation,and time to menstruous restoration were statistically lower in the hysteroscopy group than those in the laparoscopy group [(109 ±59)ml vs.(143 ±63)ml,t =-2.121 ,P =0.038;(43.7 ±17.5)min vs.(100.8 ±18.8)min,t =-1 1 .974,P =0.000;(3.8 ±0.7)d vs.(4.5 ±0.6)d,t =-4.057,P =0.000;(43.8 ±3.9)d vs.(45.9 ±3.8)d,t =-2.070,P =0.043].There were no significant differences in postoperative drainage of uterine cavity and β-hCG level resolution time between the two groups [(22.1 ±2.8)ml vs.(23.6 ±3.2)ml,t =-1 .904,P =0.062;(20.5 ±7.7)d vs.(22.9 ±9.3)d,t =-1 .075,P =0.287]. Conclusion Hysteroscopy has advantages of less intraoperative blood loss,shorter hospitalization time and quicker recovery in the treatment of endogenous type CSP.

Objective: To observe the effects of needling Baihui (GV 20), Shuigou (GV 26) and Shenmen (HT 7) on cerebral glucose metabolism in patients with vascular dementia (VD) by cerebral functional imaging technique. Methods: Twenty-five patients with VD were divided into 5 groups (Group A, B, C, D and E) randomly. Patients in the Group A were treated by needling routine acupoints for hemiplegia (Acupoints of the three yang meridians of hand and foot), and besides the routine acupoints, patients in the Group B were treated by needling Baihui(GV 20), the Group C by Shuigou(GV 26), the Group D by Shenmen(HT 7), and the Group E by Baihui(GV 20), Shuigou(GV 26), and Shenmen(HT 7). All the patients were examined by Positron Emission Tomography (PET) to detect the cerebral glucose metabolism in the bilateral frontal lobes(orbital gyri), parietal lobes, temporal lobes(hippocampus and hippocampal gyrus), occipital lobes, thalamus, lentiform nucleus, caudate nuclei, cingulate gyms and cerebellum before treatments and after treatments. Results: After needling the routine acupoints for hemiplegia, glucose metabolism increased in lentiform nucleus and temporal lobe;needling Baihui(GV 20), increased in frontal lobe, temporal lobe and lentiform nucleus;needling Shuigou(GV 26), increased in frontal lobe, thalamus and lentiform nucleus; needling Shenmen(HT 7), increased in parietal lobe and lentiform nucleus; and needling these three acupoints, increased in frontal lobe, temporal lobe, thalamus and lentiform nucleus. Conclusion:Needling Baihui(GV 20), Shuigou(GV 26) and Shenmen(HT 7) affect glucose metabolism in different functional regions of the brain, and Baihui(GV 20), Shuigou(GV26) and Shenmen(HT 7) relate to different functional regions of the brain.