1.Molecular genetic analysis of non-transferable antimicrobial resistance of shigella isolates.
Sung Yong SEOL ; Young Chul KWON ; Je Chul LEE ; Yoo Chul LEE ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1992;27(2):125-141
No abstract available.
Molecular Biology*
;
Shigella*
2.Molecular Genetic Characteristics of Trimethoprim Resistance in Clinical and Normal Fecal Isolates of Escherichia coli.
Sung Yong SEOL ; Dong Taek CHO ; Yoo Chul LEE ; Haeng Seop SHIN ; Neung Hee KIM
Journal of the Korean Society for Microbiology 1999;34(4):347-361
One hundred and thirty trimethoprim-resistant R plasmids derived from of Escherichia coli isolated from clinical specimens and feces of healthy collegians were examined for incompatibility, EcoRI endonuclease restriction fragment pattern, and Southern hybridization with DHFR I, II, III, V, and VII probe. 1. Most trimethoprim-resistant R plasmids were resistant to ampicillin, tetracycline, chloramphenicol, gentamicin, and kanamycin, and showed multiple drug resistance and various antimicrobial resistance patterns. 2. Trimethoprim-resistant R plasmids ranged from 90 to 50 kilobase and 42.3% of R plasmids tested were classified to incompatibilty group Inc FI, Inc FII or Inc FIV, 3. Among 48 random selected R plasmids from various origin, 14 R plasmids (including 9 of 14 Inc FII plasmids and 3 of 14 Inc FI plasmids) hybridized with DHFR VII oligonucleotide probe but others did not respond to any of DHFR probes used. 4. Most R plasmids showed various EcoRI endonuclease fragments and different reaction sites by Southern hybridization. Six plasmids showed identical or nearly identical molecular weight, EcoRI endonuclease fragment patterns and different sites of Southern hybridization. But 2 Inc FII plasmids derived from urine and feces showed identical pattern. These findings, if confirmed by further studies, suggest that normal flora E. coli can act as reservoir of resistant genes and, consequently, as a factor in the dissemination of these genes among enteric pathogens and need to be examined further.
Ampicillin
;
Chloramphenicol
;
Deoxyribonuclease EcoRI
;
Drug Resistance, Multiple
;
Escherichia coli*
;
Escherichia*
;
Feces
;
Gentamicins
;
Immunodeficiency Virus, Feline
;
Kanamycin
;
Molecular Biology*
;
Molecular Weight
;
Plasmids
;
R Factors
;
Tetracycline
;
Trimethoprim Resistance*
;
Trimethoprim*
3.In vivo characterization of virulence associated gene expression: II. plasmid-encoded aerobactin production and transferableantimicrobial resistance in E. coli.
Sang Hwa LEE ; Yoo Chul LEE ; Sung Yong SEOL ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1991;26(5):463-477
No abstract available.
Gene Expression*
;
Virulence*
4.Binding of fibronectin to staphylococcus aureus.
Jung Wan KIM ; Sang Hwa LEE ; Yoo Chul LEE ; Sung Yong SEOL ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1993;28(6):431-441
No abstract available.
Fibronectins*
;
Staphylococcus aureus*
;
Staphylococcus*
5.Phenotypic characterization of pseudomonas aeruginosa by pyocin typing of two different methods.
Chang Ho LEE ; Yoo Chul LEE ; Dong Taek CHO ; Sung Yong SEOL
Journal of the Korean Society for Microbiology 1991;26(2):135-146
No abstract available.
Pseudomonas aeruginosa*
;
Pseudomonas*
;
Pyocins*
6.Virulence factors and related plasmids of shigella.
Dong Taek CHO ; Sung Yong SEOL ; Yoo Chul LEE ; Sang Hwa LEE ; Je Chul LEE ; Jung Min KIM
Journal of the Korean Society for Microbiology 1992;27(6):501-515
No abstract available.
Plasmids*
;
Shigella*
;
Virulence Factors*
;
Virulence*
7.Expression and control of virulence factors associated with adherence of escherichia coli to HeLa cells.
Je Chul LEE ; Yoo Chul LEE ; Jung Min KIM ; Sang Hwa LEE ; Sung Yong SEOL ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1993;28(5):329-345
No abstract available.
Escherichia coli*
;
Escherichia*
;
HeLa Cells*
;
Humans
;
Virulence Factors*
;
Virulence*
8.Epidemiology of Serratia marcescens Isolates by Transferable Resistance Gene Analysis.
Sung Yong SEOL ; Dong Taek CHO ; Yoo Chul LEE ; Haeng Seop SHIN ; Hee Kyung CHANG ; Kyung Sook KIM
Journal of the Korean Society for Microbiology 1998;33(5):485-497
Conjugative R plasmids derived from 74 clinical isolates of Serratia marcescens were epidemiologically analyzed for antimicrobial resistance, EcoRI restriction endonuclease analysis and Southern hybridization with DHFR, TEM and SHV probe. 1. Resistance frequency of isolates against various B-lactam antibiotics was changed by year. 2. Twenty (27%) resistant strains transferred 32 R plasmids to E. coli or Klebsiella by mixed culture. Most strains isolated from 1994 to 1996 transferred only trimethoprim resistance but most strains isolated from 1997 did resistances against gentamicin (Gm) and B-lactams including ampicillin (Ap), carbenicillin (Cb), cefazolin (Cz), cefaloridine (Cl), cefamandole (Cn). 3. Ten plasmids of GmApCbCzC1Cn or GmApCbCzC1 pattern and 3 plasmids of TcSuGmTbApCbCzC1 pattern respectively showed identical EcoRI restriction endonuclease digestion patterns and hybridized fragment patterns with TEM-1 probe by Southern hybridization. These results indicate that the epidemic plasmids carrying blamM gene were present in this hospital in 1997 and molecular genetic analysis of R plasmids can be used to discriminate S. marcescens isolates for epidemiologic studies.
Ampicillin
;
Anti-Bacterial Agents
;
Carbenicillin
;
Cefamandole
;
Cefazolin
;
Cephaloridine
;
Digestion
;
DNA Restriction Enzymes
;
Epidemiologic Studies
;
Epidemiology*
;
Gentamicins
;
Klebsiella
;
Molecular Biology
;
Plasmids
;
R Factors
;
Serratia marcescens*
;
Serratia*
;
Trimethoprim Resistance
9.Epidemiological Study of Serratia marcescens Isolates by Molecular Analysis.
Sung Yong SEOL ; Dong Taek CHO ; Yoo Chul LEE ; Haeng Seop SHIN ; Hee Kyung CHANG ; Sung Joe BAE
Journal of the Korean Society for Microbiology 1997;32(5):503-520
Ninety-two strains of Serratia marcescens isolated from 5 hospitals were analyzed for plasmid profile, antimicrobial drug resistance pattern, biotyping, and production of pigment. Ninety-three percents of strains were resistant to chloramphenicol (Cm), tetracycline (Tc), sulfisoxazole (Su), cefazolin (Cz), ampicillin (Ap), and rifampin (Rf). A majority of strains were susceptible to amikacin (Ak), ciprofloxacin (Ci), and cefotaxim (Ct). Fifty-four resistance patterns were found in 94 strains and the most prevalent resistance pattern was CmTcSuApCzRf. Seventeen (17.4%) isolates could transfer their partial resistance to E. coli or Klebsiella pneumoniae by conjugation. Twenty-seven plasmid profiles in 54 strains (58.7%) were detected, however no predominant patterns were seen in isolates from each hospital. Eleven biotypes were detected. The common types were A3b (29.4%) and A8b (27.1%), predominant types were found in each hospital. Twenty strains from 4 of 5 hospitals showed consistence of 3 types. These results indicate that plasmid profile analysis, Grimont biotyping, and resistance pattern type of strains in combination are useful as an epidemiological tool for S. marcescens isolates and some of isolates were confirmed as nosocomial strains.
Amikacin
;
Ampicillin
;
Cefazolin
;
Cefotaxime
;
Chloramphenicol
;
Ciprofloxacin
;
Drug Resistance, Microbial
;
Epidemiologic Studies*
;
Klebsiella pneumoniae
;
Plasmids
;
Rifampin
;
Serratia marcescens*
;
Serratia*
;
Sulfisoxazole
;
Tetracycline
10.Molecular Epidemiologic Analysis of Enterobacter Isolated from Clinical Specimen.
Sung Yong SEOL ; Dong Taek CHO ; Yoo Chul LEE ; Haeng Seop SHIN ; Hee Kyung CHANG ; Ki Shik SHIN
Journal of the Korean Society for Microbiology 1997;32(5):487-502
Eighty-nine isolates of Enterobacter spp. from two university hospitals were analyzed by phenotypic and genotypic characteristics for epidemiologic investigation. Most strains were isolated from sputum, urine, wound, pus and catheter tip. Most isolates of Enterobacter spp. were resistant to ampicillin, cefazolin and cefoxitin and 39% of E. cloacae isolates were also resistant to other cephalosporins and aminoglycoside antibiotics except amikacin but all strains were highly susceptible to imipenem and ciprofloxacin. Twenty-six antimicrobial resistance patterns were obtained from E. clacae, but E. aerogenes showed only 4 patterns. Fourty-two plasmid profiles were identified, but plasmid was not detected from 28.4% of E. cloacae and 58% of E. aerogenes. Six biotypes from E. cloacae and three biotypes from E. aerogenes were obtained by carbohydrate metabolism. Fourteen strains of E. cloacae carried conjugative R plasmids and these plasmids were further analyzed. Among them, ten plasmids showed identical antibiogram, molecular weight, and pI value by isoelectric focusing and nearly identical restriction endonuclease fragment pattern. Their parental strains had identical antibiogram, biotype, plasmid profile, and were isolated from 4 different specimens including 6 catheter tips of different patients. But most clinical isolates showed various types of combination and seemed to be different strains. These results indicate that the epidemic strain were present in this hospital and the combination of antibiogram and plasmid analysis can be used to discriminate the epidemic strains of multi-resistant E. cloacae.
Amikacin
;
Ampicillin
;
Anti-Bacterial Agents
;
Carbohydrate Metabolism
;
Catheters
;
Cefazolin
;
Cefoxitin
;
Cephalosporins
;
Ciprofloxacin
;
Cloaca
;
DNA Restriction Enzymes
;
Enterobacter*
;
Hospitals, University
;
Humans
;
Imipenem
;
Isoelectric Focusing
;
Microbial Sensitivity Tests
;
Molecular Weight
;
Parents
;
Plasmids
;
R Factors
;
Sputum
;
Suppuration
;
Wounds and Injuries