Animals ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hypertension ; drug therapy ; pathology ; physiopathology ; Myocardial Infarction ; drug therapy ; pathology ; physiopathology ; Phytotherapy ; Ventricular Remodeling ; drug effects

Acupuncture Therapy ; Humans ; Male ; Middle Aged ; Osteonecrosis ; diagnosis ; therapy

Objective To observe the distribution and imaging of radiolabelled arginine-glycineaspartic(RGD)peptide(99Tcm-3P4-RGD2)in collagen-induced arthritis(CIA),and to investigate the possibility of new markers for monitoring the angiogenesis of rheumatoid arthritis(RA)before and after treatment by single photon emission computed tomography(SPECT)labelled with 99Tcm-3P4-RGD2.Methods Type Ⅱ chicken collagen was injected into the tails of Wistar rats to establish arthritis models.The CIA-Wistar rats were randomly divided into three group:the Avastin group,the recombinant human tumor necrosis factor-α receptor Ⅱ group,and the blank control group.The changes of the arthritis index,joint pad thickness and radiological of T/NT before and after treatment were observed.The level of vascular endothelial growth factor(VEGF)in the serum was measured by enzyme-linked immuno sorbent assay(ELISA).The back metapedes pathological section was analyzed by Hematoxylin and Eosin stain(HE)and Safranin O stain as well as arthritic pathology index was also scored.The synovial expression level of VEGF was measured by immuno-histochemistry.The stain level of VEGF was assayed by semiquantitative approach.T test was applied for the comparison of two intergroup samples.Results Abnormal radioactive concentration appeared in the arthritis of the CIA-Wistar rats(1.40±0.17,1.32±0.20,1.30±0.08,P＜0.05).Through the treatment of Avastin and recombinant human tumor necrosis factor-α receptor Ⅱ,the radiological of T/NT decreased significantly(0.43±0.14,0.40±0.12,t=17.710,16.812,P＜0.05).After the treatment with Avastin and recombinant human tumor necrosis factor-α receptor Ⅱ,the severity of synovial pannus,joint pad thickness,arthritis index and the expression of VEGF were all decreased.The radiological of T/NT was positively correlated with the indices mentioned above(r=0.753,0.800,0.892,all P＜0.01 respectively with the the rhTNFR:Fc group)(r=0.701,P=0.001;r=0.502,P=0.024;r=0.481,P=0.032 individually with the rhTNFR:Fc group).Conclusion When 99Tcm-3P4-RGD2 is injected through the tail vein of Wistar rats,it can show the pathological changes of arthritis clearly.99Tcm-3P4-RGD2may be the new eikonogen to monitor the angiogenesis of RA before and after treatment.

AIM:To evaluate the effect of all capsule polishing with polishing mode for decreasing the occurrence of posterior capsular opacification( PCO) .
METHODS: Totally 194 eyes ( 162 patients ) were performed phacoemulsification, posterior chamber intraocular lens were implanted. One hundred eyes were performed with all capsule polishing with polishing mode;94 eyes were performed with the polishing apparatus.
RESULTS:The patients were followed up for 12mo. Six eyes ( 6. 0%) of PCO were found in the 100 eyes with polishing mode group; 15 eyes ( 16. 0%) of PCO were found in the 94 eyes with the polishing apparatus group. The difference was significant (P<0. 05).
CONCLUSION: It can decrease the occurrence of PCO by performing all capsule polishing with polishing mode.

Objective:To observe the clinical efficacy of acupuncture with medication for post-stroke depression.Methods:A total of 96 patients with post-stroke depression were randomized into an observation group and a control group,with 48 cases in each group.The control group was treated with oral fluoxetine hydrochloride capsules,and the observation group was treated with acupuncture on the basis of the treatment of the control group.After 6 months of treatment,the traditional Chinese medicine (TCM) symptom scores,and Hamilton depression scale (HAMD) component scores and total scores of the two groups before and after treatment were observed,and the clinical efficacy and adverse reaction rate were compared between the two groups.Results:The total effective rate was 95.8％ in the observation group and 83.3％ in the control group,and the difference between the two groups was statistically significant (P＜0.05).After treatment,the intra-group differences in TCM symptom scores and HAMD score of both groups were statistically significant (all P＜0.01).In the observation group,the scores of indifferent expression,emotional restlessness,and sentimentality in the TCM symptoms were statistically different from those in the control group (all P＜0.01).The scores of desperate factor and sleep disorder factor and total score in HAMD in the observation group were significantly different from those in the control group (all P＜0.01).The incidence of adverse reactions was 6.3％ in the observation group and 4.2％ in the control group,and the difference was not significant between the two groups (P＞0.05).Conclusion:Acupuncture plus medication is an effective method for post-stroke depression,and it can further improve the patient's bad mood.

Humans ; Medicine, Chinese Traditional ; methods ; Thrombocytopenia ; etiology ; therapy

Drug-Eluting Stents ; adverse effects ; Humans ; Male ; Middle Aged ; Thrombosis ; etiology

Objective To explore the expression of PRR11(Proline-rich protein 11) in human osteosarcoma and investigate the effect of PRR11 on the proliferation of human osteosarcoma cells.Methods Immunohistochemical staining was applied to detect the PRR11 expression in 75 cases of osteosarcoma and corresponding normal tissues.Western blotting was used to examine PRR11 protein expression levels in osteosarcoma cell lines.We used siRNA to knock down the expression of PRR11 and tested the effects of PRR11 down-regulation on the proliferation in SaOS2 cells.Results PRR11 was overexpressed in osteosarcoma specimens compared to their paired normal tissues,the over expression rate of PRR11 in osteosarcoma and corresponding paracancerous tissues were 76％(57/75) and 9.33％(7/75) with statistical difference(P<0.05).The high expression of PRR11 was correlated with tumor pathological grade and lymphatic metastasis(P<0.05).PRR11 was expressed in 4 osteosarcoma cell lines which were SaOS2,143B,U2OS and MG63 respectively,the expression was highest in SaOS2 cells.Silencing PRR11 inhibited cell growth as compared with control cells(P<0.05).Conclusion PRR11 is overexpression in human osteosarcoma and promotes its progression by enhancing proliferation.The increased expression of PRR11 in osteosarcoma is a new target for treatment and early diagnosis of human osteosarcoma patients.

Objective To evaluate the interventional of bare-handed core muscle strength training and the sling exercise therapy (SET) on low back pain.Methods The soldiers of an Air Force Station who suffered from low back pain participated in the study.One hundred and forty-four patients were equally divided into three groups: the core muscle strength group (using core muscle strength training and the general physical therapy), the SET group (using SET and general physical therapy), and the control group (using the general physical therapy).All the groups were tested for 6 weeks.Questionnaires were distributed to the three groups before intervention, two weeks, four weeks and six weeks after intervention in order to find out about the degree of LBP, lumbar function and activity of these cases.Changes in the lumbar muscle strength were observed.Results The number of the patients who completed the test included 43 cases in the bare-handed core muscle strength group, 43 cases in the SET group and 44 cases in the control group, respectively, the VAS scores were 3.25, the Roland scores were 5.83,the FFD scores were 8.58 of the bare-handed core muscle strength group;the VAS scores were 3.30, the Roland scores were 5.93,the FFD scores were 8.10 of the SET group;the VAS scores were 2.41, the Roland scores were 3.03,the FFD scores were 7.40 of the control group.The LBP intervention efficiency of the three groups was 90.90%, 85.13% and 46.82%, respectively.There was no significant difference in indexes between the test groups(P>0.05).But compared with the control group,there was significant difference(P<0.05).Conclusion The bare-handed core muscle strength training and SET have obvious effects on LBP cases.According to their own infrastructure and facilities, military health care organizations in different environments can intervene in LBP cases with the bare-handed core muscle strength training or SET to alleviate the pain effectively.

Objective To study the risk factors of hematoma enlargement in acute hypertensive cerebral hemorrhage (AHCH) patients within 24 hours after onset. Methods A retrospective review of clinical data of consecutive patients with AHCH who met the inclusion and exclusion criteria and admitted from Mar. 2008 to Mar. 2013 was performed. The patients' data included patients' demography, previous medical history, clinical features, findings of CT, results of laboratory examinations, and the use of traditional medicines for promoting blood circulation. Patients were divided into hematoma enlargement group and non-hematoma-enlargement group. Univariate analysis was performed on the above factors first, and then with the statistically significant factors used as independent variables, hematoma growth as dependent variables, logistic regression analysis was performed to investigate the possible independent relevant factors for the early enlargement of hematoma in AHCH patients. The risk factors and enlargement of hematoma served as independent variables, the data of mRS scale obtained from 3-month follow up as dependent variables, logistic regression was then performed to investigate the influence of acute hematoma enlargement during 3-month follow up in AHCH patients. mRS 0-2 was assigned as good recovery, and mRS 3-6 as serious disability or death. The inspection level was ct=0.05. Results Among 256 patients, 43 (16.8%) were found to have hematoma enlargement. Univariate analysis showed the risk factors led to hematoma enlargement in AHCH patients were gender (male), Glasgow coma scale at admission, NIHSS (National Institute of Health Stroke Scale) at admission, course of disease, and liver function (AST). However, only two factors, namely gender (male) and course of disease, were the independent risk factors of the hematoma enlargement in AHCH patients according to the multivariate regression analysis. In addition, logistic regression revealed that the hematoma enlargement was the independent risk factor influencing the final outcome of AHCH patients, and the hematoma volume, NIHSS, and course of disease were the independent risk factors influencing the outcome of 3 month follow up. ConclusionsA male AHCH patient with shorter duration from onset to admission (within 2 hours) should alert attending physician there would be a risk of hematoma expansion. Larger amount of bleeding, enlarged hematoma, higher NIHSS and shorter duration from onset to admission herald a poor prognosis.